Cell laboratory

Waste management of

• Cells
• Media
• Antibiotics and other media addition agents that entail a risk to health and environment
• Contaminated plastic equipment

Protective Equipment

• Gloves
• Laboratory coat
• Safety glasses

Procedure for disposal

• 2M NaOH(2ml/100ml) is added to used media, mixed in well and allowed to stand for at least two minutes. The medium can be disposed of down the sink and the bottle rinsed with water before it is thrown into the ordinary waste. Alternatively, used media can be collected in bottles and handled as toxic waste.
• Cell lines and media that contain additives of infectious or toxic/carcinogenic substances must be handled as toxic waste and disposed of in yellow toxic waste containers. If cytostatics are used, the declaration label must contain the name of the substance.
• Antibiotics that have no medium added, and that it is not possible to inactivate by boiling or by using NaOH, must be handled as hazardous waste.
• Pipette tips, tubes, cell plates, culture bottles and other disposable equipment that has been in contact with biological material are disposed of in yellow toxic waste containers.
• Soiled bench paper is disposed of in yellow toxic waste containers.