Yuchuan Li

• Dalen, Knut Tomas & Li, Yuchuan (2024). Regulation of lipid droplets and cholesterol metabolism in adrenal cortical cells. Vitamines and Hormones. ISSN 0083-6729. 124, p. 79–136. doi: 10.1016/bs.vh.2023.06.007.
• Doncheva, Atanaska Ivanova; Li, Yuchuan; Khanal, Prabhat; Hjorth, Marit; Kolset, Svein Olav & Norheim, Frode Amador [Show all 8 contributors for this article] (2023). Altered hepatic lipid droplet morphology and lipid metabolism in fasted Plin2-null mice. Journal of Lipid Research. ISSN 0022-2275. 64(12). doi: 10.1016/j.jlr.2023.100461. Full text in Research Archive
• Li, Yuchuan; Amrutkar, Manoj; Finstadsveen, Anette V.; Dalen, Knut Tomas; Verbeke, Caroline Sophie & Gladhaug, Ivar Prydz (2023). Fatty acids abrogate the growth-suppressive effects induced by inhibition of cholesterol flux in pancreatic cancer cells. Cancer Cell International. ISSN 1475-2867. 23. doi: 10.1186/s12935-023-03138-8. Full text in Research Archive
• Li, Yuchuan; Khanal, Prabhat; Norheim, Frode; Hjorth, Marit; Bjellaas, Thomas & Drevon, Christian A [Show all 9 contributors for this article] (2021). Plin2 deletion increases cholesteryl ester lipid droplet content and disturbs cholesterol balance in adrenal cortex. Journal of Lipid Research. ISSN 0022-2275. 62. doi: 10.1016/J.JLR.2021.100048. Full text in Research Archive
• Li, Yuchuan; Torp, May-Kristin; Norheim, Frode; Khanal, Prabhat; Kimmel, Alan R. & Stensløkken, Kåre-Olav [Show all 8 contributors for this article] (2020). Isolated Plin5-deficient cardiomyocytes store less lipid droplets than normal, but without increased sensitivity to hypoxia . Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. ISSN 1388-1981. 1866(4). doi: 10.1016/j.bbalip.2020.158873. Full text in Research Archive Show summary

  Plin5 is abundantly expressed in the heart where it binds to lipid droplets (LDs) and facilitates physical interaction between LDs and mitochondria. We isolated cardiomyocytes from adult Plin5+/+ and Plin5-/- mice to study the role of Plin5 for fatty acid uptake, LD accumulation, fatty acid oxidation, and tolerance to hypoxia. Cardiomyocytes isolated from Plin5-/- mice cultured with oleic acid stored less LDs than Plin5+/+, but comparable levels to Plin5+/+ cardiomyocytes when adipose triglyceride lipase activity was inhibited. The ability to oxidize fatty acids into CO2 was similar between Plin5+/+ and Plin5-/- cardiomyocytes, but Plin5-/- cardiomyocytes had a transient increase in intracellular fatty acid oxidation intermediates. After pre-incubation with oleic acids, Plin5-/- cardiomyocytes retained a higher content of glycogen and showed improved tolerance to hypoxia compared to Plin5+/+. In isolated, perfused hearts, deletion of Plin5 had no important effect on ventricular pressures or infarct size after ischemia. Old Plin5-/- mice had reduced levels of cardiac triacylglycerides, increased heart weight, and apart from modest elevated expression of mRNAs for beta myosin heavy chain Myh7 and the fatty acid transporter Cd36, other genes involved in fatty acid oxidation, glycogen metabolism and glucose utilization were essentially unchanged by removal of Plin5. Plin5 seems to facilitate cardiac LD storage primarily by repressing adipose triglyceride lipase activity without altering cardiac fatty acid oxidation capacity. Expression of Plin5 and cardiac LD content of isolated cardiomyocytes has little importance for tolerance to acute hypoxia and ischemia, which contrasts the protective role for Plin5 in mouse models during myocardial ischemia.

• Mariero, Lars Henrik; Torp, May-Kristin; Heiestad, Christina Mathisen; Baysa, Anton; Li, Yuchuan & Valen, Guro [Show all 8 contributors for this article] (2019). Inhibiting nucleolin reduces inflammation induced by mitochondrial DNA in cardiomyocytes exposed to hypoxia and reoxygenation. British Journal of Pharmacology. ISSN 0007-1188. 176(22), p. 4360–4372. doi: 10.1111/bph.14830. Full text in Research Archive
• Lund, Jenny; Helle, Siw A; Li, Yuchuan; Løvsletten, Nils Gunnar; Stadheim, Hans Kristian & Jensen, Jørgen [Show all 9 contributors for this article] (2018). Higher lipid turnover and oxidation in cultured human myotubes from athletic versus sedentary young male subjects. Scientific Reports. ISSN 2045-2322. 8(1). doi: 10.1038/s41598-018-35715-7. Full text in Research Archive Show summary

  In this study we compared fatty acid (FA) metabolism in myotubes established from athletic and sedentary young subjects. Six healthy sedentary (maximal oxygen uptake (VO2max)≤ 46 ml/kg/min) and six healthy athletic (VO2max> 60 ml/kg/min) young men were included. Myoblasts were cultured and diferentiated to myotubes from satellite cells isolated from biopsy of musculus vastus lateralis. FA metabolism was studied in myotubes using [14C]oleic acid. Lipid distribution was assessed by thin layer chromatography, and FA accumulation, lipolysis and re-esterifcation were measured by scintillation proximity assay.Gene and protein expressions were studied. Myotubes from athletic subjects showed lower FA accumulation, lower incorporation of FA into total lipids, triacylglycerol (TAG), diacylglycerol and cholesteryl ester, higherTAG-related lipolysis and re-esterifcation, and higher complete oxidation and incomplete β-oxidation of FA compared to myotubes from sedentary subjects. mRNA expression of the mitochondrial electron transport chain complex III gene UQCRB was higher in cells from athletic compared to sedentary. Myotubes established from athletic subjects have higher lipid turnover and oxidation compared to myotubes from sedentary subjects.Our fndings suggestthat cultured myotubes retain some of the phenotypic traits of their donors.

• Feng, Yuan Zeng; Lund, Jenny; Li, Yuchuan; Knabenes, Irlin; Bakke, Siril Skaret & Kase, Eili Tranheim [Show all 11 contributors for this article] (2017). Loss of perilipin 2 in cultured myotubes enhances lipolysis and redirects the metabolic energy balance from glucose oxidation towards fatty acid oxidation. Journal of Lipid Research. ISSN 0022-2275. 58(11), p. 2147–2161. doi: 10.1194/jlr.M079764. Full text in Research Archive
• Li, Yuchuan; Hessvik, Nina Pettersen; Danbolt, Niels Christian & Holen, Torgeir (2016). A large-scale quantitative EM study on activation of olfactory glands shows no effect of cholinergic agents. Journal of Electron Microscopy. ISSN 0022-0744. 65(5), p. 438–443. doi: 10.1093/jmicro/dfw028. Show summary

  Little is known about olfactory glands' regulation despite their presumed importance for normal functioning of the cilia of olfactory neurons. The aim of this study was to establish an assay for olfactory gland activation by using large-scale quantitative electron microscopy (EM). In addition we wanted to test the hypothesis that cholinergic drugs activate the olfactory glands, by using our newly established EM assay. In total, over 70 000 secretory gland vesicles were quantified in over 3000 cells. Olfactory gland cell size (40.8 µm2 ± 2.0 SD), vesicle diameter (812 nm ± 57 SD) and vesicles per cell (21.6 ± 4.2 SD) were also quantified. The vesicle percentage of the cell area varied between 24% and 30%. In a blinded study we found no significant effects of cholinergic agents on parameters of vesicle number or vesicle diameter. Unexpectedly, pilocarpine treatment increased olfactory gland size, probably by inducing cell swelling. In conclusion, we have established a quantitative EM assay for olfactory gland activation and provided new data on basic olfactory gland cell characteristics. By using the EM assay, olfactory glands are shown not to be activated by cholinergic agents, which indicates an alternative regulation pathway or constitutive secretion from olfactory glands.

• Zhou, Yun; Waanders, LF; Holmseth, Silvia; Guo, C; Berger, Urs V & Li, Yuchuan [Show all 9 contributors for this article] (2014). Proteome analysis and conditional deletion of the EAAT2 glutamate transporter provide evidence against a role of EAAT2 in pancreatic insulin secretion in mice. Journal of Biological Chemistry. ISSN 0021-9258. 289(3), p. 1329–1344. doi: 10.1074/jbc.M113.529065.
• Li, Yuchuan; Lee, Sindre; Langleite, Torgrim Mikal; Norheim, Frode; Pourteymour, Shirin & Jensen, Jørgen [Show all 13 contributors for this article] (2014). Subsarcolemmal lipid droplet responses to a combined endurance and strength exercise intervention. Physiological Reports. ISSN 2051-817X. 2(11). doi: 10.14814/phy2.12187.
• Zhou, Yun; Waanders, Leonie; Holmseth, Silvia; Guo, Caiying; Berger, Urs V & Li, Yuchuan [Show all 9 contributors for this article] (2013). Proteome analysis and conditional deletion of the EAAT2 glutamate transporter provide evidence against a role of EAAT2 in pancreatic insulin secretion in mice. Journal of Biological Chemistry. ISSN 0021-9258. doi: 10.1074/jbc.M113.529065.
• Li, Yuchuan; Zhou, Yun & Danbolt, Niels Christian (2012). The Rates of Postmortem Proteolysis of Glutamate Transporters Differ Dramatically between Cells and between Transporter Subtypes. Journal of Histochemistry and Cytochemistry. ISSN 0022-1554. 60(11), p. 811–821. doi: 10.1369/0022155412458589.

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• Doncheva, Atanaska Ivanova; Li, Yuchuan; Khanal, Prabhat & Dalen, Knut Tomas (2022). Perilipin 2 regulates hepatic lipid content and lipid droplet size during fasting .

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