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NORBRAIN Two-photon Laser Scanning Microscopy

Two-photon laser scanning microscopy is a fluorescence imaging technique that allows fast and minimally invasive imaging of living tissue.

Two-photon micrograph

Photo: Letten Centre, UiO.

NORBRAIN is a large-scale national infrastructure project with funding for the next-generation equipment needed to address the basic principles of brain operation. The two-photon laser scanning microscopy has status as core facility.

Compared to confocal microscopy, two-photon laser scanning microscopy offers the advantages of deeper tissue penetration, less photo damage outside the focal region, inherent optical slicing and less chromatic aberration.

Two-photon laser scanning microscopy can be used to image the structure and function of brain cells in living research animals (most commonly mice and rats). For example, the technology enables imaging of calcium signaling at the synaptic level, even on the millisecond time scale, cerebral blood flow (after intravascular injection of fluorescent dye), brain fluid dynamics (after injection of fluorescent tracer in the cerebrospinal fluid), and brain metabolism (NADH fluorescence).


We are located at Domus Medica, Sognsvannsveien 9, 0372 Oslo

More information

More information can be found on the NORBRAIN web pages.

Published June 24, 2016 11:59 AM - Last modified Oct. 4, 2021 2:23 PM