Bacteria Lab

Waste management of

  • Bacteria
  • Contaminated plastic equipment
  • Contaminated agar plates
  • Contaminated media
  • Contaminated glass equipment
  • Kits
  • Isopropanol
  • Ethanol

Protective Equipment

  • Gloves
  • Laboratory coat
  • Safety glasses

Procedure for disposal

  • Contaminated disposable equipment and agar plates must be handled as toxic waste. Alternatively, they can be autoclaved in a double autoclave bag and then treated as ordinary waste. Pipette tips and other articles that can pierce holes in the bag must be placed in a separate container, e.g. an empty medium bottle.
  • Contaminated glass equipment must be autoclaved, rinsed and washed in the usual way.
  • Used media are autoclaved in bottles placed in a double autoclave bag and then poured down the sink. Rinse well afterwards to prevent bacterial growth in the sink and the water trap. Alternatively used media can be collected in bottles and handled as toxic waste.
  • Kits: waste must be handled in accordance with the HSE data sheet that comes with the kit.
  • Isopropanol/ethanol: for plasmid isolation isopropanol and ethanol that are used in the procedure are collected in bottles/special containers. Small volumes are disposed of in closed containers placed in yellow toxic waste containers. Large volumes such as those after maxiprep must be collected into containers and handled as hazardous waste.
  • Plasmids and other vectors are disposed of in yellow toxic waste containers.
Published Apr. 12, 2016 11:42 AM - Last modified Oct. 13, 2016 8:56 AM