Kåre-Olav Stensløkken

• Bogdanova, Mariia; Kostina, Aleksandra; Zabirnyk, Arsenii; Malashicheva, Anna; Enayati, a; Kaljusto, Mari-Liis; Kvitting, John Peder Escobar; Dissen, Erik; Sullivan, Gareth; Kostareva, Anna; Stensløkken, Kåre-Olav; Rutkovskiy, Arkady & Vaage, Jarle (2019). Interstitial cells in calcified aortic valves have reduced differentiation potential and stem cell-like properties.. Scientific Reports.  ISSN 2045-2322.  10(9) . doi: 10.1038/s41598-019-49016-0 Full text in Research Archive. Show summary

  Valve interstitial cells (VICs) are crucial in the development of calcific aortic valve disease. The purpose of the present investigation was to compare the phenotype, differentiation potential and stem cell-like properties of cells from calcified and healthy aortic valves. VICs were isolated from human healthy and calcified aortic valves. Calcification was induced with osteogenic medium. Unlike VICs from healthy valves, VICs from calcified valves cultured without osteogenic medium stained positively for calcium deposits with Alizarin Red confirming their calcific phenotype. Stimulation of VICs from calcified valves with osteogenic medium increased calcification (p = 0.02), but not significantly different from healthy VICs. When stimulated with myofibroblastic medium, VICs from calcified valves had lower expression of myofibroblastic markers, measured by flow cytometry and RT-qPCR, compared to healthy VICs. Contraction of collagen gel (a measure of myofibroblastic activity) was attenuated in cells from calcified valves (p = 0.04). Moreover, VICs from calcified valves, unlike cells from healthy valves had lower potential to differentiate into adipogenic pathway and lower expression of stem cell-associated markers CD106 (p = 0.04) and aldehyde dehydrogenase (p = 0.04). In conclusion, VICs from calcified aortic have reduced multipotency compared to cells from healthy valves, which should be considered when investigating possible medical treatments of aortic valve calcification.

• Rutkovskiy, Arkady; Lund, M; Siamansour, Tanja; Reine, Trine M.; Kolset, Svein Olav; Sand, Kristin Larsen; Ignatieva, E; Gordeev, ML; Stensløkken, Kåre-Olav; Valen, Guro; Vaage, Ingvar Jarle & Malashicheva, A (2019). Mechanical stress alters the expression of calcification-related genes in vascular interstitial and endothelial cells.. Interactive Cardiovascular and Thoracic Surgery.  ISSN 1569-9293.  28(5), s 803- 811 . doi: 10.1093/icvts/ivy339
• Torp, May-Kristin; Yang, Kuan; Ranheim, Trine; Lauritzen, Knut Husø; Alfsnes, Katrine; Vinge, Leif Erik; Aukrust, Pål; Stensløkken, Kåre-Olav; Yndestad, Arne & Sandanger, Øystein (2019). Mammalian target of rapamycin (mTOR) and the proteasome attenuates IL-1β expression in primary mouse cardiac fibroblasts. Frontiers in Immunology.  ISSN 1664-3224.  10 . doi: 10.3389/fimmu.2019.01285 Full text in Research Archive.
• Baysa, Anton; Fedorov, Anton; Kondratov, Kirill; Ruusalepp, Arno; Minasian, Sarkis; Galagudza, Michael; Popov, Maxim; Kurapeev, Dmitry; Yakovlev, Anatoly; Valen, Guro; Kostareva, Anna; Vaage, Ingvar Jarle & Stensløkken, Kåre-Olav (2018). Release of mitochondrial and nuclear DNA during on-pump heart surgery: Kinetics and relation to extracellular vesicles. Journal of Cardiovascular Translational Research.  ISSN 1937-5387.  s 1- 9 . doi: 10.1007/s12265-018-9848-3
• Bogdanova, Mariia; Kostina, Aleksandra; Zihlavnikova, Katarina; Zabirnyk, Arsenii; Malashicheva, Anna; Stensløkken, Kåre-Olav; Sullivan, Gareth; Kaljusto, Mari-Liis; Kvitting, John Peder Escobar; Kostareva, Anna; Vaage, Ingvar Jarle & Rutkovskiy, Arkady (2018). Inflammation and Mechanical Stress Stimulate Osteogenic Differentiation of Human Aortic Valve Interstitial Cells. Frontiers in Physiology.  ISSN 1664-042X.  9 . doi: 10.3389/fphys.2018.01635
• Bliksøen, Marte; Rutkovskiy, Arkady; Vaage, Ingvar Jarle & Stensløkken, Kåre-Olav (2017). Mode of perfusion influences infarct size, coronary flow and stress kinases in the isolated mouse heart. Acta Physiologica.  ISSN 1748-1708.  220(1), s 36- 46 . doi: 10.1111/apha.12773 Full text in Research Archive. Show summary

  Aim: The isolated, retrogradely perfused heart (modified Langendorff model) is a widely used method in experimental heart research. The presence of an intraventricular balloon is necessary to get functional measurements. We have previously shown that the balloon induces phosphorylation of some suggested cardioprotective mitogen-activated protein kinases (MAPK): P38-MAPK, ERK 1/2 and JNK. We hypothesized that the balloon could influence cardioprotection, protect against ischaemia reperfusion injury and interfere with coronary flow. Methods and Results: Isolated mouse hearts were perfused for 5, 10, 20, 40 and 60 min with a balloon in the left ventricle. We found a wavelike phosphorylation of all MAPK while AKT displayed a gradual dephosphorylation when compared to non-perfused hearts. Hearts were subjected to 20 min of stabilization with or without the balloon, followed by 35 min of ischaemia and 120 min of reperfusion. Although the MAPK were phosphorylated, the infarcts were larger in the balloon group. When the balloon was present during the entire protocol, compared to removal at the end of ischaemia, the infarct size was also larger, especially in the endocardial layer. The balloon reduced post-ischaemic endocardial coronary flow, despite a higher average flow, indicating a hyperperfused epicard. Blocking the balloon-induced ERK 1/2 phosphorylation during stabilization did not affect infarct size. The effect of post-conditioning was influenced by the balloon, showing reduced infarct size when the balloon was present. Conclusion: The balloon used for pressure measurements may contributes to cell death possibly by reducing endocardial coronary flow. Keywords coronary flow, kinases, Langendorff, perfusion, post-conditioning, pre-conditioning.

• Fagernes, Cathrine Elisabeth; Stensløkken, Kåre-Olav; Kjendseth, Åsmund Røhr; Berenbrink, Michael; Ellefsen, Stian & Nilsson, Göran Erik (2017). Extreme anoxia tolerance in crucian carp and goldfish through neofunctionalization of duplicated genes creating a new ethanol- producing pyruvate decarboxylase pathway. Scientific Reports.  ISSN 2045-2322.  (7) . doi: 10.1038/s41598-017-07385-4 Full text in Research Archive.
• Lefevre, Sjannie; Stecyk, Jonathan Anthony William; Torp, May-Kristin; Løvold, Lisa Yuen; Sørensen, Christina; Johansen, Ida Beitnes; Stensløkken, Kåre-Olav; Couturier, Christine Stephanie; Sloman, Katherine A. & Nilsson, Göran Erik (2017). Re-oxygenation after anoxia induces brain cell death and memory loss in the anoxia-tolerant crucian carp. Journal of Experimental Biology.  ISSN 0022-0949.  220(21), s 3883- 3895 . doi: 10.1242/jeb.165118 Full text in Research Archive.
• Rutkovskiy, Arkady; Malashicheva, Anna; Sullivan, Gareth; Bogdanova, Mariia; Kostareva, Anna; Stensløkken, Kåre-Olav; Fiane, Arnt E & Vaage, Ingvar Jarle (2017). Valve interstitial cells: The key to understanding the pathophysiology of heart valve calcification. Journal of the American Heart Association.  ISSN 2047-9980.  6(9), s 1- 24 . doi: 10.1161/JAHA.117.006339
• Bliksøen, Marte; Mariero, Lars Henrik; Torp, May-Kristin; Baysa, Anton; Ytrehus, Kirsti; Haugen, Fred; Seljeflot, Ingebjørg; Vaage, Ingvar Jarle; Valen, Guro & Stensløkken, Kåre-Olav (2016). Extracellular mtDNA activates NF-κB via toll-like receptor 9 and induces cell death in cardiomyocytes. Basic Research in Cardiology.  ISSN 0300-8428.  111(42) . doi: 10.1007/s00395-016-0553-6
• Bliksøen, Marte; Baysa, Anton; Eide, Lars; Bjørås, Magnar; Suganthan, Rajikala; Vaage, Ingvar Jarle; Stensløkken, Kåre-Olav & Valen, Guro (2015). Mitochondrial DNA damage and repair during ischemia-reperfusion injury of the heart. Journal of Molecular and Cellular Cardiology.  ISSN 0022-2828.  78, s 9- 22 . doi: 10.1016/j.yjmcc.2014.11.010
• Nilsson, Göran Erik; Vaage, Ingvar Jarle & Stensløkken, Kåre-Olav (2015). Oxygen- and temperature-dependent expression of survival protein kinases in crucian carp (carassius carassius) heart and brain. American Journal of Physiology. Regulatory Integrative and Comparative Physiology.  ISSN 0363-6119.  308(1), s R50- R61
• Stensløkken, Kåre-Olav; Ellefsen, Stian; Vasieva, Olga; Fang, Youhan; Farrell, Anthony P.; Olohan, Lisa; Vaage, Ingvar Jarle; Nilsson, Göran Erik & Cossins, Andrew R. (2014). Life without Oxygen: Gene Regulatory Responses of the Crucian Carp (Carassius carassius) Heart Subjected to Chronic Anoxia. PLOS ONE.  ISSN 1932-6203.  9(11) . doi: 10.1371/journal.pone.0109978 Full text in Research Archive.
• Rutkovskiy, Arkady; Bliksøen, Marte; Hillestad, Vigdis; Amin, Mubashar Mohammad; Czibik, Gabor Tamas; Valen, Guro; Vaage, Ingvar Jarle; Amiry-Moghaddam, Mahmood & Stensløkken, Kåre-Olav (2013). Aquaporin-1 in cardiac endothelial cells is downregulated in ischemia, hypoxia, and cardioplegia. Journal of Molecular and Cellular Cardiology.  ISSN 0022-2828.  56, s 22- 33 . doi: 10.1016/j.yjmcc.2012.12.002
• Ellefsen, Stian; Bliksøen, Marte; Rutkovskiy, Arkady; Johansen, Ida Beitnes; Kaljusto, Mari-Liis; Nilsson, Göran Erik; Vaage, Ingvar Jarle & Stensløkken, Kåre-Olav (2012). Per-unit-living tissue normalization of real-time RT-PCR data in ischemic rat hearts. Physiological Genomics.  ISSN 1094-8341.  44(12), s 651- 656 . doi: 10.1152/physiolgenomics.00004.2012
• Mariero, Lars Henrik; Rutkovskiy, Arkady; Stensløkken, Kåre-Olav & Vaage, Ingvar Jarle (2012). Hyperoxia during early reperfusion does not increase ischemia-reperfusion injury. European Journal of Cardio-Thoracic Surgery.  ISSN 1010-7940.  41(1), s 149- 153 . doi: 10.1016/j.ejcts.2011.04.022
• Rutkovskiy, Arkady; Mariero, Lars Henrik; Nygård, Ståle; Stensløkken, Kåre-Olav; Valen, Guro & Vaage, Ingvar Jarle (2012). Transient hyperosmolality modulates expression of cardiac aquaporins. Biochemical and Biophysical Research Communications - BBRC.  ISSN 0006-291X.  425(1), s 70- 75 . doi: 10.1016/j.bbrc.2012.07.052
• Rutkovskiy, Arkady; Stensløkken, Kåre-Olav; Mariero, Lars Henrik; Skrbic, Biljana; Amiry-Moghaddam, Mahmood; Hillestad, Vigdis; Valen, Guro; Perreault, Marie-Claude; Ottersen, Ole Petter; Gullestad, Lars; Dahl, Christen Peder & Vaage, Ingvar Jarle (2012). Aquaporin-4 in the heart: expression, regulation and functional role in ischemia. Basic Research in Cardiology.  ISSN 0300-8428.  107, s 280 . doi: 10.1007/s00395-012-0280-6
• Røsjø, Helge; Stridsberg, Mats; Florholmen, Geir; Stensløkken, Kåre-Olav; Ottesen, Anett Hellebø; Sjaastad, Ivar; Husberg, Cathrine; Dahl, Mai Britt; Øie, Erik; Louch, William Edward; Omland, Torbjørn & Christensen, Geir Arve (2012). Secretogranin II; a Protein Increased in the Myocardium and Circulation in Heart Failure with Cardioprotective Properties. PLOS ONE.  ISSN 1932-6203.  7(5) . doi: 10.1371/journal.pone.0037401
• Ellefsen, Stian & Stensløkken, Kåre-Olav (2010). Gene-family profiling: a normalization-free real-time RT-PCR approach with increased physiological resolution. Physiological Genomics.  ISSN 1094-8341.  42(1), s 1- 4 . doi: 10.1152/physiolgenomics.00196.2009
• Kaljusto, Mari-Liis; Rutkovskiy, Arkady; Stensløkken, Kåre-Olav & Vaage, Ingvar Jarle (2010). Postconditioning in mouse hearts is inhibited by blocking the reverse mode of the sodium - calcium exchanger. Interactive Cardiovascular and Thoracic Surgery.  ISSN 1569-9293.  10, s 743- 748 . doi: 10.1510/icvts.2009.217083
• Stensløkken, Kåre-Olav; Ellefsen, Stian; Larsen, Helene Kile; Vaage, Ingvar Jarle & Nilsson, Göran Erik (2010). Expression of heat shock proteins in anoxic crucian carp (Carassius carassius): support for cold as a preparatory cue for anoxia. American Journal of Physiology. Regulatory Integrative and Comparative Physiology.  ISSN 0363-6119.  298(6), s R1499- R1508 . doi: 10.1152/ajpregu.00675.2009
• Ellefsen, Stian; Stensløkken, Kåre-Olav; Fagernes, Cathrine Elisabeth; Kristensen, Tom & Nilsson, Gøran Erik (2009). Expression of genes involved in GABAergic neurotransmission in anoxic crucian carp brain (Carassius carassius). Physiological Genomics.  ISSN 1094-8341.  36(2), s 61- 68 . doi: 10.1152/physiolgenomics.90301.2008
• Ellefsen, Stian; Stensløkken, Kåre-Olav; Fagernes, Cathrine; Kristensen, Tom A. & Nilsson, Göran E. (2009). Expression of genes involved in GABAergic neurotransmission in anoxic crucian carp (Carassius carassius) brain. Physiological Genomics.  ISSN 1094-8341.  (36), s 61- 68
• Stensløkken, Kåre-Olav; Rutkovskiy, Arkady; Kaljusto, Mari-Liis; Hafstad, Anne Dragøy; Larsen, Terje Steinar & Vaage, Ingvar Jarle (2009). Inadvertent phosphorylation of survival kinases in isolated perfused hearts: a word of caution. Basic Research in Cardiology.  ISSN 0300-8428.  104, s 412- 423
• Bliksøen, Marte; Kaljusto, Mari-Liis; Vaage, Ingvar Jarle & Stensløkken, Kåre-Olav (2008). Effects of hydrogen sulphide on ischaemia-reperfusion injury and ischaemic preconditioning in the isolated, perfused rat heart. European Journal of Cardio-Thoracic Surgery.  ISSN 1010-7940.  34, s 344- 349 . doi: 10.1016/j.ejcts.2008.03.017
• Ellefsen, Stian; Sandvik, Guro K.; Larsen, Helene K.; Stensløkken, Kåre-Olav; Hov, Dag Are S.; Kristensen, Tom A. & Nilsson, Göran E. (2008). Expression of genes involved in excitatory neurotransmission in anoxic crucian carp (Carassius carassius) brain. Physiological Genomics.  ISSN 1094-8341.  (35), s 5- 17
• Ellefsen, Stian; Sandvik, Guro Katrine; Larsen, Helene Kile; Stensløkken, Kåre-Olav; Hov, Dag Are Steenhoff; Kristensen, Tom & Nilsson, Gøran Erik (2008). Expression of genes involved in excitatory neurotransmission in anoxic crucian carp (Carassius carassius) brain. Physiological Genomics.  ISSN 1094-8341.  35, s 5- 17
• Kaljusto, Mari-Liis; Stensløkken, Kåre-Olav; Mori, Tomohisa; Panchenko, A; Frantzen, Marthe-Lise; Valen, Guro & Vaage, Jarle (2008). Preconditioning effects of steroids and hyperoxia on cardiac ischemia-reperfusion injury and vascular reactivity. European Journal of Cardio-Thoracic Surgery.  ISSN 1010-7940.  33 . doi: 10.1016/j.ejcts.2007.12.017
• Stensløkken, Kåre-Olav; Ellefsen, Stian; Stecyk, Jonathan A. W.; Dahl, Mai Brit; Nilsson, Göran E. & Vaage, Jarle (2008). Differential regulation of AMP-activated kinase and AKT kinase in response to oxygen availability in crucian carp (Carassius carassius). American Journal of Physiology. Regulatory Integrative and Comparative Physiology.  ISSN 0363-6119.  s 1803- 1814
• Stensløkken, Kåre-Olav; Ellefsen, Stian; Stecyk, Jonathan Anthony William; Dahl, Mai Britt; Nilsson, Gøran Erik & Vaage, Jarle (2008). Differential regulation of AMP-activated kinase and AKT kinase in response to oxygen availability in crucian carp (Carassius carassius). American Journal of Physiology. Regulatory Integrative and Comparative Physiology.  ISSN 0363-6119.  295(6), s R1803- R1814 . doi: 10.1152/ajpregu.90590.2008
• Stensløkken, Kåre-Olav; Milton, SL; Lutz, PL; Sundin, L; Renshaw, GMC; Stecyk, Jonathan Anthony William & Nilsson, Gøran Erik (2008). Effect of anoxia on the electroretinogram of three anoxia-tolerant vertebrates. Comparative Biochemistry and Physiology A.  ISSN 1095-6433.  150, s 395- 403 . doi: 10.1016/j.cbpa.2008.03.022
• Stecyk, Jonathan AW; Stensløkken, Kåre-Olav; Nilsson, Gøran Erik & Farell, Anthony P (2007). Adenosine does not save the heart of anoxia-tolerant vertebrates during prolonged oxygen deprivation. Comparative Biochemistry and Physiology A.  ISSN 1095-6433.  147, s 961- 973 . doi: 10.1016/j.cbpa.2007.03.002
• Stensløkken, Kåre-Olav; Sundin, Lena & Nilsson, Gøran Erik (2006). Endothelin receptors in teleost fishes: cardiovascular effects and branchial distribution. American Journal of Physiology. Regulatory Integrative and Comparative Physiology.  ISSN 0363-6119.  290
• Stensløkken, Kåre-Olav; Sundin, Lena; Renshaw, Gillian M. C. & Nilsson, Gøran Erik (2004). Adenosinergic and cholinergic control mechanisms during hypoxia in the epaulette shark (Hemiscyllium ocellatum), with emphasis on branchial circulation. Journal of Experimental Biology.  ISSN 0022-0949.  207, s 4451- 4461
• Stecyk, Jonathan A. W.; Stensløkken, Kåre-Olav; Farrell, Anthony P. & Nilsson, Gøran Erik (2004). Maintained cardiac pumping in anoxic crucian carp. Science.  ISSN 0036-8075.  306, s 77- 77
• Stensløkken, Kåre-Olav; Sundin, Lena & Nilsson, Gøran Erik (2002). Cardiovascular effects of prostaglandin F2a and prostaglandin E2 in Atlantic cod (Gadus morhua). Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology.  ISSN 0174-1578.  172, s 363- 369
• Stensløkken, Kåre-Olav; Sundin, Lena & Nilsson, Gøran Erik (1999). Cardiovascular and gill microcirculatory effects of endothelin-1 in Atlantic cod: evidence for pillar cell contraction. Journal of Experimental Biology.  ISSN 0022-0949.  202, s 1151- 1157 Show summary

  Endothelin-1 (ET-1) has been shown to cause a considerable increase in the vascular resistance of fish gills. In trout, recent evidence suggest that this is the result of pillar cell contraction in the gill lamellae. Using epi-illumination microscopy to observe the gill lamellae of anaesthetised Atlantic cod (Gadus morhua), we here show that ET-1 (100 ng kg-1, injected into the ventral aorta) causes an enlargement of the pillar cell diameter, consistent with pillar cell contraction, and a shift of intralamellar blood flow from the lamellar sheet to the outer marginal channels. Simultaneously, there was an increase in ventral aortic blood pressure, a fall in cardiac output, an increase in gill vascular resistance, and a fall in the oxygen tension of venous blood. All these effects were blocked by the ETA/ETB receptor antagonist bosentan (5 mg kg-1). Pillar cell contraction is likely to be a mechanism for matching the functional respiratory surface area with the momentary respiratory needs

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• Ytrehus, Kirsti; Larsen, Terje; Christensen, Geir Arve; Stensløkken, Kåre-Olav & Levy, Finn Olav (2015). In memory of Guro Valen (1960-2014). Journal of Molecular and Cellular Cardiology.  ISSN 0022-2828.  79, s 254- 255 . doi: 10.1016/j.yjmcc.2014.11.026
• Bliksoen, M; Rutkovskiy, Arkady; Mariero, Lars Henrik; Vaage, Ingvar Jarle & Stensløkken, Kåre-Olav (2010). Phosphorylation of survival kinases is not a guarantee for protection of ischemia-reperfusion injury in the isolated, perfused mouse heart. Cardiovascular Research.  ISSN 0008-6363.  87, s S85- S85
• Ellefsen, Stian & Stensløkken, Kåre-Olav (2010). Gene-family profiling : a normalization-free real-time RT-PCR approach with increased physiological resolution.
• Mariero, Lars Henrik; Rutkovskiy, Arkady; Stensløkken, Kåre-Olav & Vaage, Ingvar Jarle (2010). May hyperoxic ventilation during early reperfusion increase infarct size?. Cardiovascular Research.  ISSN 0008-6363.  87, s S134- S134
• Rutkovskiy, Arkady; Mariero, Lars Henrik; Stensløkken, Kåre-Olav; Valen, Guro & Vaage, Ingvar Jarle (2010). Osmotic pressure regulates expression levels of cardiac aquaporins 1 and 4. Cardiovascular Research.  ISSN 0008-6363.  87, s S104- S104
• Røsjø, Helge; Stridsberg, M.; Florholmen, Geir; Stensløkken, Kåre-Olav; Sjaastad, Ivar; Husberg, Cathrine; Dahl, Mai Britt; Øie, Erik; Omland, Torbjørn & Christensen, Geir Arve (2010). Secretoneurin is a novel peptide increased in the myocardium and circulation in heart failure with cardioprotective properties. European Heart Journal.  ISSN 0195-668X.  31, s 314- 314
• Røsjø, Helge; Stridsberg, M; Florholmen, Geir; Stensløkken, Kåre-Olav; Sjaastad, Ivar; Husberg, Cathrine; Dahl, Christen Peder; Øie, Erik; Omland, Torbjørn & Christensen, Geir Arve (2010). Secretoneurin: a novel protective agent increased in the myocardium and circulation in heart failure. Hjerteforum.  ISSN 0802-1465.  23(1), s 101- 102
• Røsjø, Helge; Stridsberg, Mats; Florholmen, Geir; Stensløkken, Kåre-Olav; Sjaastad, Ivar; Husberg, Cathrine; Dahl, Mai Britt; Øie, Erik; Omland, Torbjørn & Christensen, Geir Arve (2010). Secretoneurin is a novel peptide increased in the myocardium and circulation in heart failure with cardioprotective properties.
• Couturier, Christine; Nilsson, Gøran Erik; Prentice, HM; Milton, SL; Fagernes, Cathrine Elisabeth; Stecyk, Jonathan Anthony William; Stensløkken, Kåre-Olav; Sandvik, Guro Katrine & Ellefsen, Stian (2009). Gene expression in anoxic turtle brain. Comparative Biochemistry and Physiology A.  ISSN 1095-6433.  153A(2), s S101- S101 . doi: 10.1016/j.cbpa.2009.04.127
• Stecyk, Jonathan A. W.; Stensløkken, Kåre-Olav; Farrell, Anthony & Nilsson, Gøran Erik (2005). The beat goes on: The heart of anoxic crucian carp. The FASEB Journal.  ISSN 0892-6638.  19(4), s A213- A214
• Stensløkken, Kåre-Olav; Sundin, Lena; Renshaw, Gillian M. C. & Nilsson, Gøran Erik (2003). Effects of hypoxia and acetylcholine on branchial circulation in epaulette shark (Hemiscyllium ocellatum). Comparative Biochemistry and Physiology A.  ISSN 1095-6433.  134, s S21- S21

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