Santosh Phuyal

• Phuyal, Santosh; Kasem, Mayes; Knittelfelder, Oskar; Sharma, Animesh; Fonseca, Davi de Miranda; Vebraite, Vaineta; Shaposhnikov, Sergey; Slupphaug, Geir; Skaug, Vidar & Zienolddiny, Shanbeh (2018). Characterization of the proteome and lipidome profiles of human lung cells after low dose and chronic exposure to multiwalled carbon nanotubes. Nanotoxicology.  ISSN 1743-5390.  12(2), s 138- 152 . doi: 10.1080/17435390.2018.1425500 Show summary

  The effects of long-term chronic exposure of human lung cells to multi-walled carbon nanotubes (MWCNT) and their impact upon cellular proteins and lipids were investigated. Since the lung is the major target organ, an in vitro normal bronchial epithelial cell line model was used. Additionally, to better mimic exposure to manufactured nanomaterials at occupational settings, cells were continuously exposed to two non-toxic and low doses of a MWCNT for 13-weeks. MWCNT-treatment increased ROS levels in cells without increasing oxidative DNA damage and resulted in differential expression of multiple anti- and pro-apoptotic proteins. The proteomic analysis of the MWCNT-exposed cells showed that among more than 5000 identified proteins; more than 200 were differentially expressed in the treated cells. Functional analyses revealed association of these differentially regulated proteins to cellular processes such as cell death and survival, cellular assembly, and organization. Similarly, shotgun lipidomic profiling revealed accumulation of multiple lipid classes. Our results indicate that long-term MWCNT-exposure of human normal lung cells at occupationally relevant low-doses may alter both the proteome and the lipidome profiles of the target epithelial cells in the lung.

• Moen, Aurora Lie; Jacobsen, Daniel; Phuyal, Santosh; Legfeldt, Anna; Haugen, Fred; Røe, Cecilie & Gjerstad, Johannes (2017). MicroRNA-223 demonstrated experimentally in exosome-like vesicles is associated with decreased risk of persistent pain after lumbar disc herniation. Journal of Translational Medicine.  ISSN 1479-5876.  15(89), s 1- 11 . doi: 10.1186/s12967-017-1194-8 Full text in Research Archive.
• Phuyal, Santosh; Kasem, Mayes; Rubio, Laura; Karlsson, Hanna; Marcos, Ricard; Skaug, Vidar & Zienolddiny, Shanbeh (2017). Effects on human bronchial epithelial cells following low-dose chronic exposure to nanomaterials: A 6-month transformation study. Toxicology in Vitro.  ISSN 0887-2333.  44, s 230- 240 . doi: 10.1016/j.tiv.2017.07.016
• Vestad, Beate; Llorente, Alicia; Neurauter, Axl; Phuyal, Santosh; Kierulf, Bente; Kierulf, Peter; Skotland, Tore; Sandvig, Kirsten; Foss, Kari Bente & Øvstebø, Reidun (2017). Size and concentration analyses of extracellular vesicles by nanoparticle tracking analysis: a variation study. Journal of Extracellular Vesicles.  ISSN 2001-3078.  6(1), s 1- 11 . doi: 10.1080/20013078.2017.1344087 Full text in Research Archive.
• Phuyal, Santosh; Skotland, Tore; Hessvik, Nina Pettersen; Simolin, Helena; Øverbye, Anders; Brech, Andreas; Parton, Robert G.; Ekroos, Kim; Sandvig, Kirsten & Llorente, Alicia (2015). The ether lipid precursor hexadecylglycerol stimulates the release and changes the composition of exosomes derived from PC-3 cells. Journal of Biological Chemistry.  ISSN 0021-9258.  290, s 4225- 4237 . doi: 10.1074/jbc.M114.593962
• Phuyal, Santosh; Hessvik, Nina Pettersen; Skotland, Tore; Sandvig, Kirsten & Llorente, Alicia (2014). Regulation of exosome release by glycosphingolipids and flotillins. The FEBS Journal.  ISSN 1742-464X.  281(9), s 2214- 2227 . doi: 10.1111/febs.12775
• Hessvik, Nina Pettersen; Phuyal, Santosh; Brech, Andreas; Sandvig, Kirsten & Llorente, Alicia (2012). Profiling of microRNAs in exosomes released from PC-3 prostate cancer cells. Biochimica et Biophysica Acta. Gene Regulatory Mechanisms.  ISSN 1874-9399.  1819(11-12), s 1154- 1163 . doi: 10.1016/j.bbagrm.2012.08.016

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• Zienolddiny, Shanbeh; Phuyal, Santosh; Kasem, Mayes; Knittelfelder, Oskar; Sharma, Animesh; Fonseca, Davi de Miranda; Vebraite, Vaineta & Shaposhnikov, Sergey (2017). Toxicoproteomic effects of carbon nanotubes on chronically exposed human lung cells. Toxicology Letters.  ISSN 0378-4274.  280, s S141 . doi: 10.1016/j.toxlet.2017.07.395
• Phuyal, Santosh; Llorente, Alicia & Sandvig, Kirsten (2014). Composition and cellular release of exosomes from cancer cells.

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