Tore Jahnsen

• Søberg, Kristoffer; Jahnsen, Tore; Rognes, Torbjørn; Skålhegg, Bjørn Steen & Lærdahl, Jon Kristen (2013). Evolutionary Paths of the cAMP-Dependent Protein Kinase (PKA) Catalytic Subunits. PLOS ONE. 8(4). doi: 10.1371/journal.pone.0060935. Full text in Research Archive Show summary

  3',5'-cyclic adenosine monophosphate (cAMP) dependent protein kinase or protein kinase A (PKA) has served as a prototype for the large family of protein kinases that are crucially important for signal transduction in eukaryotic cells. The PKA catalytic subunits Ca and Cß, encoded by the two genes PRKACA and PRKACB, respectively, are among the best understood and characterized human kinases. Here we have studied the evolution of this gene family in chordates, arthropods, mollusks and other animals employing probabilistic methods and show that Ca and Cß arose by duplication of an ancestral PKA catalytic subunit in a common ancestor of vertebrates. The two genes have subsequently been duplicated in teleost fishes. The evolution of the PRKACG retroposon in simians was also investigated. Although the degree of sequence conservation in the PKA Ca/Cß kinase family is exceptionally high, a small set of signature residues defining Ca and Cß subfamilies were identified. These conserved residues might be important for functions that are unique to the Ca or Cß clades. This study also provides a good example of a seemingly simple phylogenetic problem which, due to a very high degree of sequence conservation and corresponding weak phylogenetic signals, combined with problematic nonphylogenetic signals, is nontrivial for state-of-the-art probabilistic phylogenetic methods. Copyright: 2013 Søberg et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

• Søberg, Kristoffer; Larsen, Anja C V; Diskar, Mandy; Backe, Paul Hoff; Bjørås, Magnar & Jahnsen, Tore [Show all 10 contributors for this article] (2012). Identification and Characterization of Novel Mutations in the Human Gene Encoding the Catalytic Subunit Calpha of Protein Kinase A (PKA). PLOS ONE. 7(4). doi: 10.1371/journal.pone.0034838. Full text in Research Archive Show summary

  The genes PRKACA and PRKACB encode the principal catalytic (C) subunits of protein kinase A (PKA) Cα and Cβ, respectively. Cα is expressed in all eukaryotic tissues examined and studies of Cα knockout mice demonstrate a crucial role for Cα in normal physiology. We have sequenced exon 2 through 10 of PRKACA from the genome of 498 Norwegian donors and extracted information about PRKACA mutations from public databases. We identified four interesting nonsynonymous point mutations, Arg45Gln, Ser109Pro, Gly186Val, and Ser263Cys, in the Cα1 splice variant of the kinase. Cα variants harboring the different amino acid mutations were analyzed for kinase activity and regulatory (R) subunit binding. Whereas mutation of residues 45 and 263 did not alter catalytic activity or R subunit binding, mutation of Ser109 significantly reduced kinase activity while R subunit binding was unaltered. Mutation of Cα Gly186 completely abrogated kinase activity and PKA type I but not type II holoenzyme formation. Gly186 is located in the highly conserved DFG motif of Cα and mutation of this residue to Val was predicted to result in loss of binding of ATP and Mg2+, which may explain the kinetic inactivity. We hypothesize that individuals born with mutations of Ser109 or Gly186 may be faced with abnormal development and possibly severe disease. © 2012 Søberg et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

• Hereng, Tuva Holt; Elgstøen, Katja B. Prestø; Cederkvist, Fritjof Henning; Eide, Lars; Jahnsen, Tore & Skålhegg, Bjørn Steen [Show all 7 contributors for this article] (2011). Exogenous pyruvate accelerates glycolysis and promotes capacitation in human spermatozoa. Human Reproduction. ISSN 0268-1161. 26(12), p. 3249–3263. doi: 10.1093/humrep/der317. Full text in Research Archive
• Kvissel, Anne Katrine; Ørstavik, Sigurd; Eikvar, Sissel; Brede, Gaute; Jahnsen, Tore & Collas, Philippe [Show all 8 contributors for this article] (2007). Involvement of the catalytic subunit of protein kinase A and of HA95 in pre-mRNA splicing. PMID: 17594903 [PubMed - in process]. Experimental Cell Research. ISSN 0014-4827. 313, p. 2795–2809. doi: 10.1016/j.yexcr.2007.05.014. Full text in Research Archive
• Orstavik, Sigurd; Funderud, Ane; Hafte, Tolesa Tilahun; Eikvar, Sissel; Jahnsen, Tore & Skålhegg, Bjørn Steen (2005). Identification and characterization of novel PKA holoenzymes in human T lymphocytes. The FEBS Journal. ISSN 1742-464X. 272, p. 1559–1567. Full text in Research Archive
• Kvissel, Anne Katrine; Ørstavik, Sigurd; Øistad, P; Rootwelt, Terje; Jahnsen, Tore & Skålhegg, Bjørn Steen (2004). Induction of Cbeta splice variants and formation of novel forms of protein kinase A type II holoenzymes during retinoic acid-induced differentiation of human NT2 cells. Cellular Signalling. ISSN 0898-6568. 16(5), p. 577–587. Full text in Research Archive Show summary

  Cyclic AMP (cAMP) and cAMP-dependent protein kinase (PKA) are critical regulators of neuronal differentiation. The expression, levels and activities of PKA subunits were studied prior to and during differentiation of the human neuronal precursor cell line NTera 2 (NT2). Undifferentiated NT2 cells expressed mainly cytoplasmic PKA type I, consisting of the regulatory subunit RIalpha and the catalytic subunit Calpha. Low levels of PKA type II consisting of RIIalpha or RIIbeta associated with Calpha were also detected, mainly in the cytoplasm and in the Golgi-centrosomal area. During retinoic acid-induced differentiation, the RIalpha and RIIalpha expressions remained in the cytoplasm, while we observed a strong upregulation of RIIbeta, located to the whole cytoplasm including neurite extensions. This upregulation coincided with increased PKA-specific activity accompanied by a strong induction of a number of neuronal-specific Cbeta splice variants that together with RIIbeta form novel PKAII holoenzymes. Formation of novel PKAII holoenzymes may imply specific PKA features which may have consequences for the process of neuronal differentiation and nerve cell function

• Eide, Turid; Tasken, Kristin Austlid; Carlson, C.; Williams, G.; Jahnsen, Tore & Tasken, Kjetil [Show all 7 contributors for this article] (2003). Protein kinase A-anchoring protein AKAP95 interacts with MCM2, a regulator of DNA replication. Journal of Biological Chemistry. ISSN 0021-9258. 278(29), p. 26750–26756. Full text in Research Archive
• Ørstavik, Sigurd; Reinton, Nils Torgny Kjelkevik; Frengen, Eirik; Langeland, Bjørn Tore; Jahnsen, Tore & Skålhegg, Bjørn Steen (2001). Identification of novel splice variants of the human catalytic subunit cbeta of cAMP-dependent protein kinase. European Journal of Biochemistry (EJB). ISSN 0014-2956. 268(19), p. 5066–5073. Full text in Research Archive Show summary

  Four different isoforms of the catalytic subunit of cAMP-dependent protein kinase, termed Calpha, Cbeta,Cgamma and PrKX have been identified. Here we demonstrate that the human Cbeta gene encodes six splice variants, designated Cbeta1, Cbeta2, Cbeta3, Cbeta4, Cbeta4ab and Cbeta4abc. The Cbeta splice variants differ in their N-terminal ends due to differential splicing of four different forms of exon 1 designated exon 1-1,1-2, 1-3, 1-4 and three exons designated a, b and c. All these exons are located upstream of exon 2 in the Cbeta gene. The previously identified human Cbeta variant has been termed Cbeta1, and is similar to the Cbeta isoform identified in the mouse, ox, pig and several other mammals. Human Cbeta2, which is the homologue of bovine Cbeta2, has no homologue in the mouse. Human Cbeta3 and Cbeta4 are homologous to the murine Cbeta3 and Cbeta2 splice variants, whereas human Cbeta4ab and Cbeta4abc represent novel isofoms previously not identified in any other species. At the mRNA level, the Cbeta splice variants reveal tissue specific expression. Cbeta1 was most abundantly expressed in the brain, with low-level expression in several other tissues. The Cbeta3 and Cbeta4 splice variants were uniquely expressed in human brain in contrast to Cbeta2, which was most abundantly expressed in tissues of the immune system, with no detectable expression in brain. We suggest that the various Cbeta splice variants when complexed with regulatory subunits may give rise to novel holoenzymes of protein kinase A that may be important for mediating specific effects of cAMP.

• Martins, Sandra B.; Eide, Turid; Steen, Rikke L.; Jahnsen, Tore; Skålhegg, Bjørn Steen & Collas, Philippe (2000). HA95 is a protein of the chromatin and nuclear matrix regulating nuclear envelope dynamics. Journal of Cell Science. ISSN 0021-9533. 113, p. 3703–3713. Full text in Research Archive Show summary

  We report a role for HA95, a nuclear protein with high homology to the nuclear A-kinase anchoring protein AKAP95, in the regulation of nuclear envelope-chromatin interactions. Biochemical and photobleaching data indicate that HA95 is tightly associated with chromatin and the nuclear matrix/lamina network in interphase, and bound to chromatin at mitosis. HA95 resides in a complex together with lamin B receptor (LBR), lamina-associated polypeptide (LAP)2 and emerin, integral proteins of the inner nuclear membrane. Cross-linking experiments, however, illustrate a tight association of HA95 with LBR and LAP2 only. Intra-nuclear blocking of HA95 with anti-HA95 antibodies abolishes nuclear breakdown in a mitotic HeLa cell extract. The antibodies inhibit nuclear membrane breakdown and chromatin condensation - the latter independently of nuclear membranes. However, lamina disassembly is not affected, as judged by immunological analyses of A/C- and B-type lamins. In contrast, immunoblocking of HA95 bound to condensed chromosomes does not impair chromatin decondensation, nuclear membrane reassembly or lamina reformation. Our results argue for a role for HA95 in anchoring nuclear membranes and lamins to chromatin in interphase, and in releasing membranes from chromatin at mitosis. The data also suggest that HA95 is not involved in initial binding of membranes to chromatin upon nuclear reassembly. We propose that HA95 is a central platform at the chromatin/nuclear matrix interface implicated in regulating nuclear envelope-chromatin interactions during the cell cycle.

• Reinton, Nils Torgny Kjelkevik; Ørstavik, Sigurd; Haugen, T. B.; Tasken, Kjetil; Jahnsen, Tore & Skålhegg, Bjørn Steen (2000). A novel isoform of human cAMP-dependent protein kinase, Ca-s, localized to sperm midpiece. Biology of Reproduction. ISSN 0006-3363. 63, p. 607–611. Full text in Research Archive
• Ørstavik, Sigurd; Eide, Turid; Collas, Philippe; Han, Innoc O.; Tasken, Kjetil & Kieff, Elliot [Show all 8 contributors for this article] (2000). Identification, cloning and characterization of a novel nuclear protein, HA95, homologous to the A-kinase anchoring protein, AKAP95. Biology of the Cell. ISSN 0248-4900. 92, p. 27–37. Full text in Research Archive
• Reinton, Nils Torgny Kjelkevik; Collas, Philippe; Haugen, T. B.; Skålhegg, Bjørn Steen; Hansson, Vidar & Jahnsen, Tore [Show all 7 contributors for this article] (2000). Localization of a Novel Human A-Kinase-Anchoring Protein, hAKAP220, during Spermatogenesis. Developmental Biology. ISSN 0012-1606. 223, p. 194–204. Full text in Research Archive
• Skålhegg, Bjørn Steen; Witczak, Oliwia; Reinton, Nils Torgny Kjelkevik; Eide, Turid; Eikvar, Sissel & Collas, Philippe [Show all 9 contributors for this article] (1999). Specific effects of cAMP are mediated by multiple isozymes of cyclic AMP-dependent protein kinase and A-kinase anchoring proteins. Journal of Molecular Microbiology and Biotechnology. ISSN 1464-1801. 1, p. 49–63. Full text in Research Archive Show summary

  A large number of hormones, neurotransmitters, and other signaling substances that bind to G-protein coupled cell-surface receptors, converge their signals through one sole second messenger, 3', 5'-cyclic adenosine monophosphate (cAMP). Cyclic AMP has been shown to regulate cellular processes such as cell growth and differentiation, ion channel conductivity, synaptic release of neurotransmitters, and gene transcription. The principle intracellular target for cAMP in mammalian cells is the cAMP-dependent protein kinase (PKA). The fact that this broad specificity protein kinase mediates a number of discrete physiological responses following cAMP engagement has raised the question of how specificity is maintained in the cAMP/PKA system. It has been speculated to what extent specificity in cAMP signaling may be obtained by differential stimulation of multiple PKA holoenzymes that are targeted to various sites within a single cell through specific anchoring proteins.

• Krall, Judith; Tasken, Kjetil; Staheli, John; Jahnsen, Tore & Movsesian, Matthew A. (1999). Identification and quantitation of cAMP-dependent protein kinase R subunit isoforms in subcellular fractions of failing human myocardium. Journal of Molecular and Cellular Cardiology. ISSN 0022-2828. 31(5), p. 971–980. Full text in Research Archive Show summary

  Isoforms of regulatory (R) subunits of cAMP-dependent protein kinase were identified immunochemically and quantified in soluble and washed particulate fractions of failing human left ventricular myocardium. The predominant isoforms in both fractions were RI alpha and RII alpha. Both isoforms were present in comparable amounts in these fractions, although RII alpha subunits were somewhat more prevalent than RI alpha subunits in washed particulate fractions. The ratio of R subunits to catalytic (C) subunits was three-fold higher in soluble than in particulate fractions. Identical observations were made in preparations from non-failing human left ventricular myocardium. Since RI and RII have different affinities for cAMP and may direct catalytic activity to different substrates, the presence of both subunits in both soluble and particulate fractions provides a mechanism whereby the compartment-selective changes in cAMP content that have been described in failing human myocardium may affect not only the level but also the profile of protein phosphorylation in these compartments. The high R:C subunit ratio in soluble fractions suggests that cytosolic kinase activity in human myocardium may be less sensitive to changes in cAMP content than membrane-bound kinase activity, and this may contribute to the different effects of increases in soluble and particulate cAMP content on intracellular Ca2+transients and contraction and relaxation.

• Witczak, Oliwia; Skålhegg, Bjørn Steen; Keryer, Guy; Bornens, Michel; Tasken, Kjetil & Jahnsen, Tore [Show all 7 contributors for this article] (1999). Cloning and characterization of a cDNA encoding an A-kinase anchoring protein located in the centrosome, AKAP450. EMBO Journal. ISSN 0261-4189. 18(7), p. 1858–1868. Full text in Research Archive Show summary

  A combination of protein kinase A type II (RII) overlay screening, database searches and PCR was used to identify a centrosomal A-kinase anchoring protein. A cDNA with an 11.7 kb open reading frame was characterized and found to correspond to 50 exons of genomic sequence on human chromosome 7q21-22. This cDNA clone encoded a 3908 amino acid protein of 453 kDa, that was designated AKAP450 (DDBJ/EMBL/GenBank accession No. AJ131693). Sequence comparison demonstrated that the open reading frame contained a previously characterized cDNA encoding Yotiao, as well as the human homologue of AKAP120. Numerous coiled-coil structures were predicted from AKAP450, and weak homology to pericentrin, giantin and other structural proteins was observed. A putative RII-binding site was identified involving amino acid 2556 of AKAP450 by mutation analysis combined with RII overlay and an amphipatic helix was predicted in this region. Immunoprecipitation of RII from RIPA-buffer extracts of HeLa cells demonstrated co-precipitation of AKAP450. By immunofluorecent labeling with specific antibodies it was demonstrated that AKAP450 localized to centrosomes. Furthermore, AKAP450 was shown to co-purify in centrosomal preparations. The observation of two mRNAs and several splice products suggests additional functions for the AKAP450 gene.

• Keryer, Guy; Skålhegg, Bjørn Steen; Landmark, Brynjar Fowels; Hansson, Vidar; Jahnsen, Tore & Tasken, Kjetil (1999). Differential localization of protein kinase A type II isozymes in the Golgi-centrosomal area. Experimental Cell Research. ISSN 0014-4827. 249(1), p. 131–146. Full text in Research Archive Show summary

  Selectivity in the action of cAMP may be mediated by compartmentalized pools of cyclic AMP-dependent protein kinase (PKA). PKA type II is directed to different subcellular loci by interaction of the type II regulatory subunits (RIIalpha, RIIbeta) with A-kinase anchoring proteins. In order to separately investigate the subcellular localization of PKA type II isozymes, monospecific antibodies to human RIIalpha and RIIbeta subunits of PKA were developed. We demonstrate that centrosomes bind both RIIalpha and RIIbeta. Centrosomes were the preferred intracellular anchoring site for RIIbeta. However, centrosomal localization of RIIbeta was observed only in some highly differentiated cells such as keratinocytes, granulosa cells, and macrophages and in all neoplastic cell lines examined. Centrosomal localization of RIIbeta was not observed in normal undifferentiated cells such as fibroblasts, myoblasts, and T and B cells. In contrast, RIIalpha was abundant in the Golgi area and in the trans-Golgi network (TGN). Furthermore, although RIIalpha appeared to colocalize with microtubules in the Golgi/TGN, extractions with nonionic detergent demonstrated that RIIalpha was mainly membrane-associated. In addition, alterations of microtubule dynamics with Nocodazole or Taxol affected the distribution of the detergent-extractable pool of RIIalpha, indicating that RIIalpha may localize with microtubule-associated vesicles. Thus, RIIalpha and RIIbeta clearly localize differently in the Golgi-centrosomal region. This indicates specific roles for PKA isozymes containing either RIIalpha or RIIbeta.

• Reinton, Nils Torgny Kjelkevik; Haugen, T. B.; Ørstavik, Sigurd; Skålhegg, Bjørn Steen; Hansson, Vidar & Jahnsen, Tore [Show all 7 contributors for this article] (1998). The gene encoding the C(gamma) catalytic subunit of cAMP-dependent protein kinase is a transcribed retroposon. Genomics. ISSN 0888-7543. 49, p. 290–297. Full text in Research Archive
• Eide, Turid; Coglan, V.; Ørstavik, Sigurd; Holsve, Christian; Solberg, Rigmor & Skålhegg, Bjørn Steen [Show all 12 contributors for this article] (1998). Molecular Cloning, Chromosomal Localization and Cell Cycle-Dependent Subcellular Distribution of the A-kinase anchoring protein, AKAP95. Experimental Cell Research. ISSN 0014-4827. p. 305–316. Full text in Research Archive
• Skarpen, Ellen; Thoresen, G. Hege; Tasken, Kjetil; Samuelsen, Jon Tore; Jahnsen, Tore & Schwarze, P. E. [Show all 7 contributors for this article] (1998). Localization of cAMP-dependent signal transducers in early rat liver carcinogenesis. Histochemistry and Cell Biology. ISSN 0948-6143. 109(3), p. 203–209. Full text in Research Archive Show summary

  Cyclic AMP (cAMP) is an important regulator of liver growth and differentiation. The main intracellular cAMP receptor, cAMP-dependent protein kinase (PKA), consists of two regulatory (R) and two catalytic (C) subunits. There are two classes, RI and RII, of the regulatory subunit, giving rise to type I (RI2C2) and type II (RII2C2) PKA. The RI/RII ratio generally decreases during organ development, and increases during carcinogenesis. Alterations in this ratio have been implicated as an important factor in experimental and clinical carcinogenesis. We have studied the expression of RIalpha, RIIalpha, Calpha, and an important substrate of PKA, the cAMP-response element binding protein, during rat liver carcinogenesis. Two-color immunofluorescence and confocal laser scan microscopy were used to characterize localization of the cAMP-dependent signal transducers in hepatocytes, bile ducts, oval cells, and preneoplastic lesions. We found that bile ducts and oval cells (putative liver stem cells) contained a higher RI/RII ratio than hepatocytes and preneoplastic lesions. Thus, an altered RI/RII ratio was not detected during early rat liver carcinogenesis, but may contribute to differentiation of putative liver stem cells to hepatocytes.

• Tasken, Kjetil; Naylor, Susan L.; Solberg, Rigmor & Jahnsen, Tore (1998). Mapping of the gene encoding the regulatory subunit RIIalpha of cAMP-dependent protein kinase (locus PRKAR2A) to human chromosome region 3p21.3-p21.2. Genomics. ISSN 0888-7543. p. 378–381. Full text in Research Archive
• Witczak, Oliwia; Orstavik, Sigurd; Nataranjan, V.; Frengen, Eirik; Jahnsen, Tore & Sandberg, Mårten (1998). Characterization of the gene encoding the human type II cGMP-dependent protein kinase. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 245(1), p. 113–119. Full text in Research Archive Show summary

  The type II cGMP-dependent protein kinase (cGK) plays a pivotal role in the regulation of intestinal fluid balance in man. Furthermore, mice carrying a null mutation for the gene encoding the type II cGK develop as dwarfs indicating that this enzyme has other less characterized roles. The present report describes the isolation and characterization of bacterial artificial chromosome (BAC)- and P1-derived artificial chromosome (PAC)-clones containing the gene encoding the human type II cGK. The gene was estimated to cover at least 125 kb and consisted of 19 exons separated by introns of various lengths. The splice junctions of the type II cGK gene corresponded well with the structure of the gene encoding human type I cGK and with the splice junctions observed in the Drosophila melanogaster DG2 gene. 5'-rapid amplification of cDNA-ends established the presence of a non-translated exon.

• Foss, Kari Bente; Solberg, Rigmor; Simard, J.; Myklebust, Frode; Hansson, Vidar & Jahnsen, Tore [Show all 7 contributors for this article] (1997). Molecular cloning, upstream sequence and promoter studies of the human gene for the regulatory subunit RII alpha of cAMP-dependent protein kinase. Biochimica et Biophysica Acta. ISSN 0006-3002. 1350(1), p. 98–108. Full text in Research Archive
• Knutsen, Helle Katrine; Tasken, Kjetil; Eskild, Winnie; Richards, J.S.; Kurten, R.C. & Torjesen, Peter A. [Show all 10 contributors for this article] (1997). Characterization of the 5'-flanking region of the gene for the cAMP-inducible protein kinase A subunit, RIIbeta, in Sertoli cells. Molecular and Cellular Endocrinology. ISSN 0303-7207. 129(1), p. 101–114. Full text in Research Archive
• Tasken, Kjetil; Skålhegg, Bjørn Steen; Taskén, Kristin Austlid; Solberg, Rigmor; Knutsen, Helle Katrine & Levy, Finn Olav [Show all 16 contributors for this article] (1997). Structure, function, and regulation of human cAMP-dependent protein kinases, Advances in Second Messenger and Phosphoprotein Research. p. 191–204. Full text in Research Archive
• Reed, R.B.; Sandberg, Mårten; Jahnsen, Tore; Lohmann, Suzanne M.; Francis, S.H. & Corbin, J.D. (1997). Structural order of the slow and fast intrasubunit cGMP-binding sites of type I alpha cGMP-dependent protein kinase, Advances in Second Messenger and Phosphoprotein Research. p. 205–217. Full text in Research Archive
• Solberg, Rigmor; Sandberg, Mårten; Nataranjan, V.; Torjesen, Peter A.; Hansson, Vidar & Jahnsen, Tore [Show all 7 contributors for this article] (1997). The human gene for the regulatory subunit RIalpha of cAMP-dependent protein kinase - Two distinct promoters provide differential regulation of alternately spliced mRNAs. Endocrinology. ISSN 0013-7227. 138, p. 169–181. Full text in Research Archive Show summary

  The present study reports the exon-intron organization of the human RIalpha gene of cAMP-dependent protein kinase and approximately 2 kilobases (kb) of the 5' flanking region obtained by isolation and sequencing of several phage clones from human genomic libraries. The RIalpha gene is composed of 9 coding exons of varying lengths, separated by introns, giving the gene a total length of at least 21 kb. Our recent cloning of a processed RIalpha pseudogene (Solberg R, Sandberg M, Spurkland A, Jahnsen T (1993) Genomics 15: 591-597) with a 5'-noncoding region different from the previously reported RIalpha cDNA, indicated that the RIalpha gene may have multiple leader exons giving rise to alternately spliced mRNAs. Reverse transcription of human testis RNA followed by polymerase chain reaction identified two different RIalpha mRNA species (RIalpha1a, RIalpha1b) containing distinct 5' sequences due to alternately splicing of the gene. The previously known RIalpha1b mRNA revealed low constitutive expression in a human B lymphoid cell line (Reh) and was stimulated only 4 to 6-fold by treatment with cAMP. In contrast, very low levels of the novel RIalpha1a mRNA were present in untreated Reh cells, but were stimulated 40 to 50-fold by cAMP. The 5' flanking sequence of the RIalpha gene was G/C-rich and did not contain any TATA box. Several putative transcription initiation sites were identified in front of each leader exon (exons 1a and 1b) by the 5' rapid amplification of cDNA ends (RACE) technique. In order to determine whether the sequences 5' of both leader exons had promoter activities, the 5'-flanking sequences of exons 1a and 1b were inserted in front of a chloramphenicol acetyl transferase (CAT) reporter gene and their ability to direct transcription were examined. Transfection of these constructs into rat GH4C1 cells demonstrated that both constructs had promoter activities as evidenced by high levels of CAT activity.

• Orstavik, Sigurd; Nataranjan, V.; Tasken, Kjetil; Jahnsen, Tore & Sandberg, Mårten (1997). Characterization of the human gene encoding the type I alpha and type I beta cGMP-dependent protein kinase (PRKG1). Genomics. ISSN 0888-7543. 42, p. 311–318. Full text in Research Archive
• Orstavik, Sigurd; Solberg, Rigmor; Tasken, Kjetil; Nordahl, Marianne; Altherr, Michael R & Hansson, Vidar [Show all 8 contributors for this article] (1996). Molecular cloning, cDNA structure, and chromosomal localization of the human type II cGMP-dependent protein kinase. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 220(3), p. 759–767. Full text in Research Archive Show summary

  96183022 Institute of Medical Biochemistry, Faculty of Medicine, University of Oslo, Norway The type II cGMP-dependent protein kinase is an enzyme originally isolated from the small intestine, and is thought to be involved in the regulation of intestinal ion transport and fluid secretion. A complementary DNA clone encoding a part of the human type II cGMP-dependent protein kinase was isolated from a cerebellum library. Based on sequence information from this complementary DNA, the 5'-end of the type II cGMP-dependent protein kinase was amplified from human brain messenger RNA using polymerase chain reaction. The composite complementary DNA encoded a 762 amino acid protein with a calculated molecular mass of 87.4 kDa. Messenger RNAs encoding the type II cGMP-dependent protein kinase were detected in small intestine, colon and prostate. By using polymerase chain reaction and Southern blotting on somatic cell hybrids, the gene encoding, the type II cGMP-dependent protein kinase was mapped to human chromosome 4q13.1-q21.1

• Tasken, Kjetil; Solberg, Rigmor; Zhao, Ying; Hansson, Vidar; Jahnsen, Tore & Siciliano, Michael J. (1996). The gene for the Calpha subunit of cAMP-dependent protein kinase (locus PRKACA) localize to human chromosome region 19p13.1. Genomics. ISSN 0888-7543. 36, p. 535–538. Full text in Research Archive Show summary

  We have determined the chromosomal localization of the gene for the catalytic subunit Calpha of cAMP-dependent protein kinase (locus PRKACA) to human chromosome 19 using polymerase chain reaction (PCR) and Southern blot analysis of two different somatic cell hybrid mapping panels. In addition, PCR analysis of a chromosome 19 mapping panel revealed the presence of a human Calpha-specific amplification product only in cell lines containing the region 19p13.1 to 19q12. Finally, two color fluorescence in situ hybridization (FISH) to metaphase chromosomes using the human Calpha cDNA and human chromosome 19 inter-Alu-PCR product as probes, localized the human Calpha gene to chromosome region 19p13.1.

• Reed, R.B.; Sandberg, Mårten; Jahnsen, Tore; Lohmann, Suzanne M.; Francis, S.H. & Corbin, J.D. (1996). Fast and slow cyclic nucleotide-dissociation sites in cAMP-dependent protein kinase are transposed in type Ibeta cGMP-dependent protein kinase. Journal of Biological Chemistry. ISSN 0021-9258. 271(29), p. 17570–17575. Full text in Research Archive
• Tasken, Kjetil; Solberg, Rigmor; Foss, Kari Bente; Skålhegg, Bjørn Steen; Hansson, Vidar & Jahnsen, Tore (1995). Cyclic AMP-dependent protein kinase (vertebrates). In Hardie, G. & Hanks, S. (Ed.), The Protein Kinase FactsBook. Elsevier. ISSN 9780123247193. p. 58–63. Full text in Research Archive
• Skålhegg, Bjørn Steen; Tasken, Kjetil; Hansson, Vidar; Huitfeldt, Henrik; Jahnsen, Tore & Lea, Tor Erling (1994). Location of cAMP-dependent protein kinase type I with the TCR/CD3 complex. Science. ISSN 0036-8075. 263(5143), p. 84–87. Full text in Research Archive
• Skålhegg, Bjørn Steen; Rasmussen, Anne Marie; Tasken, Kjetil; Hansson, Vidar; Jahnsen, Tore & Lea, Tor Erling (1994). Cyclic AMP sensitive signalling by the CD28 marker requires concomitant stimulation by the T-cell antigen receptor (TCR/CD3) complex. Scandinavian Journal of Immunology. ISSN 0300-9475. 40(2), p. 201–208. Full text in Research Archive
• Meinecke, M.; Geiger, J.; Butt, E.; Sandberg, Mårten; Jahnsen, Tore & Chakraborty, T. [Show all 9 contributors for this article] (1994). Human cyclic GMP-dependent protein kinase I beta overexpression increases phosphorylation of an endogenous focal contact-associated vasodilator-stimulated phosphoprotein without altering the thrombin-evoked calcium response. Molecular Pharmacology. ISSN 0026-895X. 46(2), p. 283–290. Full text in Research Archive
• Tasken, Kjetil; Skålhegg, Bjørn Steen; Tasken, Kristin Austlid; Solberg, Rigmor; Levy, Finn Olav & Lea, Tor Erling [Show all 8 contributors for this article] (1994). Diversity and regulation of cAMP-dependent protein kinases. In Verhoeven, G. & Habenicht, U.-F. (Ed.), Molecular and Cellular Endocrinology of the Testis. Springer. ISSN 9783662221891. p. 185–217. Full text in Research Archive
• Solberg, Rigmor; Tasken, Kjetil; Wen, W.; Coglan, V.; Meinkoth, J. L. & Scott, John D. [Show all 8 contributors for this article] (1994). Human regulatory subunit RI beta of cAMP-dependent protein kinases: expression, holoenzyme formation and microinjection into living cells. Experimental Cell Research. ISSN 0014-4827. 214(2), p. 595–605. Full text in Research Archive
• Tasken, Kjetil; Andersson, Kristin Brevik; Erikstein, B. K.; Hansson, Vidar; Jahnsen, Tore & Blomhoff, Heidi Kiil (1994). Regulation of growth in a neoplastic B cell line by transfected subunits of 3',5'-cyclic adenosine monophosphate-dependent protein kinase. Endocrinology. ISSN 0013-7227. 135(5), p. 2109–2119. Full text in Research Archive
• Foss, Kari Bente; Landmark, Brynjar Fowels; Skålhegg, Bjørn Steen; Tasken, Kjetil; Jellum, Egil & Hansson, Vidar [Show all 7 contributors for this article] (1994). Characterization of in vitro translated human regulatory and catalytic subunits of cAMP dependent protein kinases. European Journal of Biochemistry (EJB). ISSN 0014-2956. 220(1), p. 217–223. Full text in Research Archive
• Solberg, Rigmor; Sandberg, Mårten; Spurkland, Anne & Jahnsen, Tore (1993). Isolation and characterization of a human pseudogene for the regulatory subunit RI alpha of cAMP-dependent protein kinases and its sublocalization on chromosome 1. Genomics. ISSN 0888-7543. 15(3), p. 591–597. Full text in Research Archive
• Tasken, Kjetil; Andersson, Kristin Brevik; Skålhegg, Bjørn Steen; Tasken, Kristin Austlid; Hansson, Vidar & Jahnsen, Tore [Show all 7 contributors for this article] (1993). Reciprocal regulation of mRNA and protein for subunits of cAMP-dependent protein kinase (RI alpha and C alpha) by cAMP in a neoplastic B cell line (Reh). Journal of Biological Chemistry. ISSN 0021-9258. 268(31), p. 23483–23489. Full text in Research Archive
• Jahnsen, Tore; Tasken, Kristin Austlid; Landmark, Brynjar Fowels; Knutsen, HK; Solberg, R. & Foss, Kari Bente [Show all 10 contributors for this article] (1993). Multiple genes for regulatrory and catalytic subunits of cyclic-AMP dependent protein kinases are differentially expressed and regulated in testicular cells. In Mornex, René; Jaffiol, C. & Leclère, J. (Ed.), Progress in endocrinology: the proceedings of the Ninth International Congress of Endocrinology, Nice 1992. Parthenon Pub. Group. ISSN 9781850704690. p. 29–33. Full text in Research Archive
• Landmark, Brynjar Fowels; Øyen, Ole; Skålhegg, Bjørn Steen; Fauske, Beate; Jahnsen, Tore & Hansson, Vidar (1993). Cellular location and age-dependent changes of the regulatory subunits of cAMP-dependent protein kinase in rat testis. Journal of Reproduction and Fertility. ISSN 0022-4251. p. 323–334. doi: 10.1530/jrf.0.0990323. Full text in Research Archive
• Tasken, Kjetil; Skålhegg, Bjørn Steen; Solberg, Rigmor; Andersson, Kristin Brevik; Taylor, S. S. & Lea, Tor [Show all 9 contributors for this article] (1993). Novel isozymes of cAMP-dependent protein kinase exist in human cells due to formation of RI alpha-RI beta heterodimeric complexes. Journal of Biological Chemistry. ISSN 0021-9258. 268(28), p. 21276–21283. Full text in Research Archive
• Skålhegg, Bjørn Steen; Landmark, Brynjar Fowels; Foss, Kari Bente; Lohmann, Suzanne M.; Hansson, Vidar & Lea, Tor Erling [Show all 7 contributors for this article] (1992). Identification, purification, and characterization of subunits of cAMP-dependent protein kinase in human testis. Reverse mobilities of human RII alpha and RII beta on sodium dodecyl sulfate-polyacrylamide gel electrophoresis compared with rat and bovine RIIs. Journal of Biological Chemistry. ISSN 0021-9258. 267(8), p. 5374–5379. Full text in Research Archive
• Skalhegg, B. S.; Landmark, Brynjar Fowels; Doskeland, Stein Ove; Hansson, Vidar; Lea, T & Jahnsen, Tore (1992). Cyclic AMP-dependent protein kinase type I mediates the inhibitory effects of 3',5'-cyclic adenosine monophosphate on cell replication in human T lymphocytes. Journal of Biological Chemistry. ISSN 0021-9258. 267(22), p. 15707–15714. Full text in Research Archive
• Simard, J.; Berube, B.; Sandberg, Mårten; Grzeschik, K. H.; Gagné, R. & Hansson, Vidar [Show all 7 contributors for this article] (1992). Assignment of the gene encoding the catalytic subunit C beta of cAMP-dependent protein kinase to the p36 band on chromosome 1. Human Genetics. ISSN 0340-6717. 88(6), p. 653–657. Full text in Research Archive
• Solberg, Rigmor; Sistonen, P.; Träskelin, A. L.; Berube, D.; Simard, J. & Krajci, Peter [Show all 8 contributors for this article] (1992). Mapping of the regulatory subunits RI beta and RII beta of cAMP-dependent protein kinase genes on human chromosome 7. Genomics. ISSN 0888-7543. 14(1), p. 63–69. Full text in Research Archive
• Ørstavik, Sigurd; Sandberg, Mårten; Berube, B.; Natarajan, V.; Simard, J. & Walter, Ulrich [Show all 9 contributors for this article] (1992). Localization of the human gene for the type I cyclic GMP-dependent protein kinase to chromosome 10. Cytogenetics and Cell Genetics. ISSN 0301-0171. 59(4), p. 270–273. Full text in Research Archive
• Lönneberg, P.; Parvinen, M.; Jahnsen, Tore; Hansson, Vidar & Persson, H. (1992). Stage Specific and Cell Specific Expression of Cyclic Adenosine 3',5' Monophosphate Dependent Protein Kinases in Rat Seminiferous Epithelium. Biology of Reproduction. ISSN 0006-3363. 46(6), p. 1057–1068. Full text in Research Archive
• Beebe, Stephen; Salomonsky, P.; Jahnsen, Tore & Li, Y. (1992). The C gamma subunit is a unique isozyme of the cAMP-dependent protein kinase. Journal of Biological Chemistry. ISSN 0021-9258. 267(35), p. 25505–25512. Full text in Research Archive
• Knutsen, Helle Katrine; Tasken, Kristin Austlid; Eskild, Winnie; Jahnsen, Tore & Hansson, Vidar (1992). Half-lives of different sized mRNAs for the PKA subunit RI alpha are regulated differently in response to inhibition of transcription and translation. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 184(1), p. 454–460. Full text in Research Archive
• Tasken, Kristin Austlid; Knutsen, Helle Katrine; Eikvar, Lars Kristian; Tasken, Kjetil; Eskild, Winnie & Jahnsen, Tore [Show all 7 contributors for this article] (1992). Protein kinase C activation by 12-O-tetradecanoylphorbol 13-acetate modulates messenger ribonucleic acid levels for two of the regulatory subunits of 3',5'-cyclic adenosine monophosphate-dependent protein kinases (RII beta and RI alpha) via multiple and distinct mechanisms. Endocrinology. ISSN 0013-7227. 130(3), p. 1271–1280. Full text in Research Archive
• Foss, Kari Bente; Simard, J.; Berube, B.; Beebe, Stephen; Sandberg, Mårten & Grzeschik, K. B. [Show all 8 contributors for this article] (1992). Localization of the Catalytic Subunit Cgamma of the cAMP-Dependent Protein Kinase Gene (PRKACG) to Human Chromosome Region 9q13. Cytogenetics and Cell Genetics. ISSN 0301-0171. 60(1), p. 22–25. Full text in Research Archive
• Landmark, Brynjar Fowels; Fauske, Beathe; Eskild, Winnie; Skålhegg, Bjørn Steen; Lohmann, Suzanne M. & Hansson, Vidar [Show all 8 contributors for this article] (1991). Identification, Characterization, and Hormonal Regulation of 3′,5′-Cyclic Adenosine Monophosphate Dependent Protein Kinases in Rat Sertoli Cells. Endocrinology. ISSN 0013-7227. 129(5), p. 2345–2354. Full text in Research Archive
• Eskild, Winnie; Ree, Anne Hansen; Levy, Finn Olav; Jahnsen, Tore & Hansson, Vidar (1991). Cellular localization of mRNAs for the retinoic acid receptor-alpha, cellular retinol-binding protein, and cellular retinoic acid-binding protein in rat testis: evidence for germ cell-specific mRNAs. Biology of Reproduction. ISSN 0006-3363. 44, p. 53–61. Full text in Research Archive
• Tasken, Kristin Austlid; Knutsen, Helle Katrine; Attramadal, Håvard; Tasken, Kjetil; Jahnsen, Tore & Hansson, Vidar [Show all 7 contributors for this article] (1991). Different Mechanisms are Involved in cAMP Mediated Induction of mRNAs for Subunits of cAMP-Dependent Protein Kinases. Molecular Endocrinology. ISSN 0888-8809. 5(1), p. 21–28. Full text in Research Archive
• Sandberg, Mårten; Natarajan, V.; Ørstavik, Sigurd; Lohmann, Suzanne M. & Jahnsen, Tore (1991). The Human Type I cGMP-Dependent Protein Kinase Gene. In Ross, E. M. & Wirtz, K. W. A. (Ed.), Biological Signal Transduction. Springer. ISSN 9783642751363. p. 301–308. Full text in Research Archive
• Knutsen, Helle Katrine; Tasken, Kristin Austlid; Eskild, Winnie; Jahnsen, Tore & Hansson, Vidar (1991). Adenosine 3',5'-monophosphate-dependent stabilization of messenger ribonucleic acids (mRNAs) for protein kinase-A (PKA) subunits in rat Sertoli cells: rapid degradation of mRNAs for PKA subunits is dependent on ongoing RNA and protein synthesis. Endocrinology. ISSN 0013-7227. 129(5), p. 2496–2502. Full text in Research Archive
• Sandberg, Mårten; Butt, E.; Nolte, C.; Fischer, L.; Halbrügge, M. & Beltman, J. [Show all 10 contributors for this article] (1991). Characterization of Sp-5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole- 3',5'-monophosphorothioate (Sp-5,6-DCl-cBiMPS) as a potent and specific activator of cyclic-AMP-dependent protein kinase in cell extracts and intact cells. Biochemical Journal. ISSN 0264-6021. 279(2), p. 521–527. Full text in Research Archive

View all works in NVA

• Kvissel, Anne Katrine; Orstavik, S; Eikvar, Sissel; Øistad, Pia Madeleine; Punga, T & Jahnsen, Tore [Show all 9 contributors for this article] (2004). Co-localization and in vitro phosphorylation of splicing factors by cAMP-dependent protein kinase. The FASEB Journal. ISSN 0892-6638. 18, p. C259–C259. Full text in Research Archive
• Kvissel, Anne Katrine; Øistad, Pia; Ørstavik, Sigurd; Jahnsen, Tore; Collas, Philippe & Skålhegg, Bjørn Steen (2002). Location of the cAMP-dependent kinase and components of the splicesosome. Full text in Research Archive
• Eskild, M.; Witczak, Oliwia; Tasken, Kjetil; Jahnsen, Tore & Skålhegg, Bjørn Steen (2001). Mapping the Golgi targeting domain of A-kinase anchoring protein, AKAP450. Full text in Research Archive
• Witczak, Oliwia; Delouvée, A.; Kemmner, W.A.; Skålhegg, Bjørn Steen; Tasken, Kjetil & Bornens, Michel [Show all 8 contributors for this article] (2001). Mapping the centrosmal targeting domain of AKAP450. Full text in Research Archive
• Eskild, M.; Witczak, Oliwia; Tasken, Kjetil; Jahnsen, Tore; Collas, Philippe & Skålhegg, Bjørn Steen (2001). Mapping the Golgi targeting domain of A-kinase anchoring protein, AKAP450. Full text in Research Archive
• Eide, Turid; Taskén, Kristin Austlid; Carlson, C. R.; Collas, Philippe; Grallert, Beata & Jahnsen, Tore [Show all 7 contributors for this article] (2001). The A-kinase anchoring protein AKAP95 interacts with MCM2, a component of the pre-replication complex. Full text in Research Archive
• Eide, Turid; Taskén, Kristin Austlid; Carlson, C. R.; Collas, Philippe; Grallert, Beata & Jahnsen, Tore [Show all 7 contributors for this article] (2000). The A-kinase anchoring protein AKAP95 interacts with MCM2, a component of the pre-replication complex. Full text in Research Archive
• Martins, Sandra B.; Eide, T.; Jahnsen, Tore; Skålhegg, Bjørn Steen & Collas, Philippe (2000). HA95 is a novel protein of the chromatin-nuclear matrix interface. Biol. Cell 92, 168. Full text in Research Archive
• Reinton, Nils Torgny Kjelkevik; Ørstavik, Sigurd; Skålhegg, Bjørn Steen; Collas, Philippe; Haugen, Trine Berit & Hansson, Vidar [Show all 8 contributors for this article] (1999). Multiplicity and targeting in protein kinase A signalling. Full text in Research Archive
• Tasken, Kjetil; Skålhegg, Bjørn Steen; Taskén, Kristin Austlid; Solberg, Rigmor; Knutsen, Helle Katrine & Levy, Finn Olav [Show all 12 contributors for this article] (1996). Specificity in cAMP-mediated signalling. Full text in Research Archive
• Reinton, Nils Torgny Kjelkevik; Tasken, Kjetil; Skålhegg, Bjørn Steen; Hansson, Vidar & Jahnsen, Tore (1996). Identification of the protein product from the Cgamma gene of cAMP-dependent protein kinase in the human testis. Full text in Research Archive
• Larsen, Turid; Holsve, Christian; Scott, John D.; Tasken, Kjetil; Solberg, Rigmor & Hansson, Vidar [Show all 7 contributors for this article] (1996). Molecular cloning of the human AKAP95 for the regulatory subunit type II of cyclic AMP-dependent protein kinase. Full text in Research Archive
• Tasken, Kjetil; Skålhegg, Bjørn Steen; Taskén, Kristin Austlid; Solberg, Rigmor; Knutsen, Helle Katrine & Levy, Finn Olav [Show all 12 contributors for this article] (1996). Specificity in cAMP-mediated signalling. Full text in Research Archive
• Reinton, Nils Torgny Kjelkevik; Tasken, Kjetil; Solberg, Rigmor; Skålhegg, Bjørn Steen; Levy, Finn Olav & Jahnsen, Tore [Show all 7 contributors for this article] (1996). The testis specific catalytic subunit Cgamma of cAMP-dependent protein kinase is a functional retroposon. Full text in Research Archive
• Solberg, Rigmor; Tasken, Kjetil; Sandberg, Mårten; Taskén, Kristin Austlid; Hansson, Vidar & Jahnsen, Tore (1996). Alternate initiation and differential regulation of two distinct promoters in the human RIalpha gene of cAMP-dependent protein kinase. Full text in Research Archive
• Keryer, Guy; Levy, Finn Olav; Dupont, Carine; Evain-Brion, Daniéle; Blomhoff, Heidi Kiil & Jahnsen, Tore [Show all 7 contributors for this article] (1996). Overexpression of RIIalpha in human B lymphocyte cells interferes with the endocytotic pathway involved in cell growth regulation. Full text in Research Archive
• Larsen, Turid; Tasken, Kjetil; Solberg, Rigmor; Skålhegg, Bjørn Steen; Hansson, Vidar & Jahnsen, Tore (1996). Cyclic AMP-regulated A-kinase anchoring proteins (AKAPs) in rat Sertoli cells. Full text in Research Archive
• Solberg, Rigmor; Tasken, Kjetil; Taskén, Kristin Austlid; Hansson, Vidar & Jahnsen, Tore (1996). Two promoters in the RIalpha gene of cyclic AMP-dependent protein kinase provide means of differential regulation. Full text in Research Archive
• Solberg, Rigmor; Tasken, Kjetil; Hansson, Vidar & Jahnsen, Tore (1996). Exon-intron organization, 5' upstream sequence and alternate splicing of mRNAs for the regulatory subunit RIalpha of cAMP-dependent protein kinases. Full text in Research Archive
• Movsesian, Matthew A.; Krall, Judy; Staheli, John; Tasken, Kjetil & Jahnsen, Tore (1996). Identification and quantitation of cAMP-dependent protein kinase regulatory subunits in normal and failing human myocardium. Full text in Research Archive
• Levy, Finn Olav; Skålhegg, Bjørn Steen; Torgersen, Knut Martin; Rasmussen, Anne Marie; Rolstad, Bent & Jahnsen, Tore [Show all 8 contributors for this article] (1996). Function and localization of cAMP-dependent protein kinase type I and II in lymphoid cells. Full text in Research Archive
• Reinton, Nils Torgny Kjelkevik; Tasken, Kjetil; Solberg, Rigmor; Levy, Finn Olav; Jahnsen, Tore & Hansson, Vidar (1996). The catalytic subunit, Cgamma, of cAMP-dependent protein kinase is a functional retroposon. Full text in Research Archive
• Larsen, Turid; Solberg, Rigmor; Scott, John D.; Tasken, Kjetil; Hansson, Vidar & Jahnsen, Tore (1996). Molecular cloning of the human AKAP95 for the regulatory subunit type II of cAMP-dependent protein kinase. Full text in Research Archive
• Solberg, Rigmor; Tasken, Kjetil; Hansson, Vidar & Jahnsen, Tore (1995). Exon-intron organization, 5' upstream sequence and alternate splicing of mRNAs for the regulatory subunit RIalpha of cAMP-dependent protein kinases. Full text in Research Archive
• Reinton, Nils; Solberg, Rigmor; Levy, Finn Olav; Tasken, Kjetil; Jahnsen, Tore & Hansson, Vidar (1995). Cloning of the gene encoding the catalytic subunit, Cgamma, of cAMP-dependent protein kinase. Full text in Research Archive
• Levy, Finn Olav; Skålhegg, Bjørn Steen; Torgersen, Knut Martin; Rasmussen, Anne Marie; Rolstad, Bent & Jahnsen, Tore [Show all 8 contributors for this article] (1995). Function and localization of cAMP-dependent protein kinase type I and II in lymphoid cells. Full text in Research Archive
• Reed, R.B.; Sandberg, Mårten; Jahnsen, Tore; Lohmann, Suzanne M.; Walter, Ulrich & Francis, S.H. [Show all 7 contributors for this article] (1995). The fast and slow cGMP-binding sites of human type Ibeta cGK are reversed compared to the cAMP-binding sites of cAK. Full text in Research Archive
• Ørstavik, Sigurd; Sandberg, Mårten; Nordahl, Marianne; Solberg, Rigmor; Tasken, Kjetil & Hansson, Vidar [Show all 7 contributors for this article] (1995). Molecular cloning and cDNA sequence of the human type II cGMP-dependent protein kinase. Full text in Research Archive
• Eide, Turid; Solberg, Rigmor; Scott, John D.; Tasken, Kjetil; Hansson, Vidar & Jahnsen, Tore (1995). Molecular cloning of the human AKAP95 for the regulatory subunit type II of cyclic AMP-dependent protein kinase (cAK). Full text in Research Archive
• Foss, Kari Bente; Landmark, Brynjar Fowels; Skålhegg, Bjørn Steen & Jahnsen, Tore (1991). Characterization of the human regulatory subunits RIIa and RIIb of cAMP-dependent protein kinase (PKA). Full text in Research Archive
• Landmark, Brynjar Fowels; Ree, Anne Hansen; Jahnsen, Tore & Hansson, Vidar (1991). Hormonal regulation of regulatory subunits of cAMP-dependent protein kinase in MCF-7 cells. Full text in Research Archive
• Skarpen, Ellen; Landmark, Brynjar Fowels; Hansson, Vidar; Rui, Hallgeir; Purvis, Ken & Jahnsen, Tore (1991). Identification, characterization and regulation of cAMP-dependent protein kinases in rat prostatic lobes. Full text in Research Archive
• Søberg, Kristoffer; Skålhegg, Bjørn Steen & Jahnsen, Tore (2017). On the evolution and function of the catalytic subunits of protein kinase A (PKA). Universitetet i Oslo. Full text in Research Archive
• Witczak, Oliwia; Jahnsen, Tore & Skålhegg, Bjørn Steen (2002). AKAP450 - a PKA anchoring protein involved in the structural and functional organization of the Golgi apparatus. Universitetet i Oslo. Full text in Research Archive
• Eide, Turid; Jahnsen, Tore & Tasken, Kjetil (2002). Diverse functions of the A-Kinase Anchoring Protein AKAP95 and the AKAP95 Homologous Protein HA95 in Nuclear dynamics. Universitetet i Oslo. Full text in Research Archive
• Ørstavik, Sigurd & Jahnsen, Tore (2000). Cyclic nucleotide-dependent protein kinase: Molecular characterization of human genes in cyclic nucleotide-dependent signal transduction. Universitetet i Oslo. Full text in Research Archive
• Foss, Kari Bente & Jahnsen, Tore (1995). Human cAMP-dependent protein kinases. Protein characterization, cloning, and chromosomal localization. Universitetet i Oslo. Full text in Research Archive
• Solberg, Rigmor & Jahnsen, Tore (1994). Human type I regulatory subunits of cyclic AMP-dependent protein kinases. Structure, function, and chromosomal localization. Universitetet i Oslo. Full text in Research Archive
• Tasken, Kjetil & Jahnsen, Tore (1994). Structure, function, and regulation of cyclic AMP-dependent protein kinases. Universitetet i Oslo. Full text in Research Archive
• Skålhegg, Bjørn Steen & Jahnsen, Tore (1993). Isozymes of cyclic AMP-dependent protein kinase in human cells. Expression, regulation and isozyme-specific effects in human T lymphocytes. Universitetet i Oslo. Full text in Research Archive
• Sandberg, Mårten & Jahnsen, Tore (1991). Molecular cloning and primary structure of mammalian cyclic nucleotide-dependent protein kinases. Universitetet i Oslo. Full text in Research Archive

View all works in NVA