Paul Johannes Helm

Senior Engineer - Glia
Image of Paul Johannes Helm
Norwegian version of this page
Phone +47 22851159
Mobile phone 412 42 733
Room 0347
Visiting address Domus Medica Sognsvannsveien 9 0372 Oslo
Postal address Postboks 1105 Blindern 0317 Oslo


1988: MSc in Nuclear Physics by Johannes-Gutenberg University, Mainz, FRG, Germany

1997: PhD in Physical Engineering by the Royal Institute of Technology, Stockholm, Sweden

Work tasks

  • Design and Installation of Systems for Optical Microsocpy, specifically confocal and non-linear laser scanning microscopy
  • Education of users


U. Dinger, S. Dutta, P.J. Helm, G. Huber, T. Kühl (1988), Laser Sättigungsspektroskopie am Ag-I-Atomstrahl, Verhandlungen der deutschen physikalischen Gesellschaft, Reihe VI, Bd.23, p.14

Paul Johannes Helm,Sättigungsspektroskopische Untersuchungen an den Hüllenspektren der stabilen Silberisotope 107Ag und 109Ag, Diploma Thesis, Johannes Gutenberg-University, Mainz, FRG, 1988

B. Ulfhake, K. Carlsson, K. Mossberg, U. Arvidsson and P. J. Helm (1991), Imaging of fluorescent neurons labelled with fluoro-gold and fluorescent axon terminals labelled with AMCA (7-amino-4-methylcoumarine-3-acetic acid) conjugated antiserum using a UV-laser confocal scanning microscope, Journal of Neuroscience Methods, Vol. 40, pp. 39-48

N. Burnashev, A. Khodorova, P. Jonas, P.J. Helm, W. Wisden, H. Monyer, P.H. Seeburg, and B. Sakmann (1992), Calcium Permeable AMPA-Kainate Receptors in Fusiform Cerebellar GlialCells, Science, Vol. 256, pp. 1566-1570

Carlsson, Kjell; Mossberg, Karin; Helm, Paul J.; Philip, Johan (1992); Use of UV-excitation for confocal fluorescence microscopy in a conventional beam-scanning instrument, Proc. SPIE Vol. 1660, p. 466-481, Biomedical Image Processing and Three-Dimensional Microscopy, Raj S. Acharya; Carol J. Cogswell; Dmitry B. Goldgof; Eds.

K. Carlsson, K. Mossberg, P.J. Helm, and J.Philip (1992), Use of UV Excitation in Confocal Laser Scanning Fluorescence Microscopy, Micron and Microscopica Acta, Vol. 23, No. 4, pp. 413-428

N. Melamed, P.J. Helm, and R. Rahamimoff (1993), Confocal Microscopy Reveals Coordinated Calcium Fluctuations and Oscillations in Synaptic Boutons, The Journal of Neuroscience, Vol.13, No.2, pp. 632-649

P.J. Helm,A confocal scanning laser microscope for quantitative measurements of the intracellular dynamics of Ca2+ ions in living biological cells,Thesis accepted by the Royal Institute of Technology, Stockholm, Sweden,as Licentiatsavhandling for the academic degree of a "TeknologieLicentiat (KTH)".TRITA-FYS-4038, Stockholm, August 1994

P.J. Helm, O. Franksson, and K. Carlsson (1995), A confocal scanning laser microscope for quantitative ratiometric 3D measurements of [Ca2+] and Ca2+ diffusions in living cells stained with Fura-2, Pflügers Archiv - European Journal of Physiology, Vol. 429, pp. 672-681

H. Markram, P.J. Helm, and B. Sakmann (1995), Dendritic calcium transients evoked by single back-propagating action potentials in rat neocortical pyramidal neurons, Journal of Physiology, Vol. 485, pp. 1-20

P.J. Helm (1996), A microscopic setup for combined, and time coordinated electrophysiological and confocal fluorescence microscopic experiments on neurons in living brain slices, Review of Scientific Instruments, Vol. 67, No.2, Feb. 1996, pp. 530-534

Ardan Patwardhan and Paul Johannes Helm (1996), A 3-D point measurement CSLM system for the study of time dynamic phenomena, TRITA-FYS 4041, ISRN/KTH/FYS/R--4041--SE

PJ Helm (1997), Dishes for confocal scanning laser microscopy on calibration solutions and living, cultured cells, TRITA-FYS 4043, ISRN/KTH/FYS/R--4043--SE

PJ Helm (1997), A confocal scanning laser microscope for cell physiological research, Thesis accepted by the Royal Institute of Technology, Stockholm, Sweden, as Doktorsavhandling for the academic degree of a "Teknologie Doktor (KTH)", i.e. PhD in Engineering Sciences. TRITA-FYS 4044, ISRN/KTH/FYS/R--4044--SE

PJ Helm, A Patwardhan, and EMM Manders (1997), A study of the precision of confocal, ratiometric, Fura-2 based [Ca2+] measurements, Cell Calcium 22(4):287-298

Linda Bergersen, Ola Wærhaug, Johannes Helm, Marion Thomas, PetterLaake, Andrew J. Davies, Mariangela C. Wilson, Andrew P. Halestrap, Ole P. Ottersen (2001), A novel postsynaptic density protein: the monocarboxylate transporterMCT2 is co-localized with δ-glutamate receptors in postsynaptic densities of parallel fiber-Purkiň cell synapses, Exp Brain Res 136:523-534

P. Johannes Helm, Finn-Mogens Haug, Johan F. Storm, Ole-Petter Ottersen (2001), Design and installation of a multi-mode microscopy system, Proc. SPIE, Vol. 4262:396-406

Gabriela Bezakova, Johannes P. Helm, Maura Francolini, and Terje Lømo (2001), Effects of Purified Recombinant Neural and Muscle Agrin on Skeletal Muscle Fibers In Vivo, The Journal of Cell Biology 153(7):1441-1452

Thomas M.J., Sjaastad I., Andersen K., Helm P.J., Wasserstrom J.A., Sejersted O.M. Ottersen O.P. (2003), Localization and function of the Na+/Ca2+-exchanger in normal and detubulated rat cardiomyocytes, Journal of Molecular & Cellular Cardiology. 35(11):1325-37

G. Nase, P.J. Helm, O.P.Ottersen (2005), Visualization of Structural Changes at the cellular and subcellular Level in the Cerebral Cortex of Live Mice, Program No. 189.9. 2005 Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience, 2005.

G. Nase, P.J. Helm, O.P.Ottersen (2005), A multiphoton laser scanning microscope setup for transcranial in vivo brain imaging on mice, Review of Scientific Instruments 76:123702-1-123702-5.

Gabriele Nase, P.Johannes Helm, Ole Petter Ottersen (2006), Structural changes at the cellular and subcellular level in the cerebral cortex of mice visualized by means of trans-cranial multi photon in vivo microscopy, Proc. SPIE, 6089:608920

G. Nase, P.J. Helm, O.P. Ottersen (2006),In vivo measurements of cell volume respons to hypo-osmotic stress, Program No. 433.1/D71 2006 Abstract Viewer/Itinerary Planner. Atlanta, GA, Society for Neuroscience, 2006.

P.J. Helm, Ole P. Ottersen , G. Nase (2007), Optical effects of the cranium in trans-cranial in vivo two photon laser scanning microscopy in mice, Proc. SPIE, 6442:644221

P.J. Helm, Ole P. Ottersen , G. Nase (2007), An improved method for the analysis of some optical properties of the mouse cranium: implications for in vivo optical imaging, Program No. 533.3/HHH27 2007, Annual Meeting of the International Society for Neuroscience, San Diego, CA, USA, Oct. 31 - Nov. 7, 2007

P.J. Helm, N.F. Proske, F. Baur (2007), Teaching and Discussing Neuroscience in Pious Christian Circles, Program No. 27.17/OOO19 2007, Annual Meeting of the International Society for Neuroscience, San Diego, CA, USA, Oct. 31 - Nov. 7, 2007

G. Nase, P.J.Helm, T. Oguchi, L.N.G. Nilsson, L. Lannfelt, O.P. Ottersen, R. Torp (2008), Local impact of perivascular plaques on cerebral blood flow dynamics in a transgenic mouse model of Alzheimer's disease, Proc. SPIE, 6860:686022

G. Nase, P.J. Helm, R. Enger & O. P. Ottersen (2008), Water Entry into Astrocytes during Brain Edema Formation, Glia 56:895-902

G. Nase, P. J. Helm, L. Nilsson, L. Lannfelt, O.P. Ottersen, R. Torp (2008), Astrocyte morphological dynamics in a mouse model of Alzheimer's disease with the Arctic and Swedish mutation, Program No. 637.25/J8, Annual Meeting of the International Society for Neuroscience, Washington, DC, USA, Nov. 15-19, 2008

P. Johannes Helm, Ole P. Ottersen, Gabriele Nase (2009), Analysis of optical properties of the mouse cranium - Implications for in vivo multi photon laser scanning microscopy, Journal of Neuroscience Methods 178:316-322

P. J. Helm, T. Reppen, P. Heggelund (2009), A setup for combined multi photon laser scanning microscopic and multi electrode patch clamp experiments on brain slices, Proc. SPIE 7183:71832Q-1 - 71832Q-8

P. Johannes Helm, Gabriele Nase, Paul Heggelund and Trond Reppen (2010), Complementary equipment for controlling multiple laser beams on single scanner MPLSM systems, Proc. SPIE 7569, 75692Y

P. Johannes Helm and Ole P. Ottersen (2011), Proposal of a new method to measure FRET quantitatively in living or fixed biomedical specimens on a laser microscope, Proc. SPIE 7903, 790331

P. Johannes Helm, Sigita Augustinaite, Trond Reppen and Paul Heggelund (2011), Simultaneous Chemical Activation and Image Scanning on Single Scanner Laser Scanning Microscope Systems, Program no. 519.25, Annual Meeting of The International Society for Neuroscience, Washington, DC, USA, Nov. 12-16, 2011

P. Johannes Helm, N. Frank Proske and Friedbert Baur (2011), Faith beyond the Neuron, Program no. 28.20SA, Annual Meeting of the International Society for Neuroscience, Washington, DC, USA, Nov. 12-16, 2011

P. Johannes Helm (2012), Recommendations for the design and the installation of large laser scanning microscopy systems, Proc. SPIE, 8226, 82262M-82262M-7

Helm P.J. (2012), Proposal of a New Method for Measuring Foerster Resonance Energy Transfer (FRET) Rapidly, Quantitatively and Non-Destructively, International Journal of Molecular Sciences 13(10):12367-12382

Xi Wu, Ji-Yao Chen, Andreas Brech, Caihong Fang, Jianfang Wang, P. Johannes Helm, Qian Peng (2013), The use of femto-second lasers to trigger powerful explosions of gold nanorods to destroy cancer cells, Biomaterials 34:6157-6162

Sigita Augustinaite,Bernd Kuhn, Paul Johannes Helm, and Paul Heggelund (2014), NMDA Spike/Plateau Potentials in Dendrites of Thalamocortical Neurons, The Journal of Neuroscience 34(33):10892-10905

Wannan Tang, Karolina Szokol, Vidar Jensen, Rune Enger, Chintan A. Trivedi, Øivind Hvalby, P. Johannes Helm, Loren L. Looger, Rolf Sprengel, and Erlend A. Nagelhus (2015), Stimulation-Evoked Ca2+ Signals in Astrocytic Processes at Hippocampal CA3-CA1 Synapses of Adult Mice Are Modulated by Glutamate and ATP, The Journal of Neuroscience 35(7):3016-3021

Rune Enger, Wannan Tang, Gry Fluge Vindedal, Vidar Jensen, P. Johannes Helm, Rolf Sprengel ,Loren L. Looger , and Erlend A. Nagelhus (2015), Dynamics of Ionic Shifts in Cortical Spreading Depression, Cerebral Cortex 25(11):4469-76

Rune Enger, Didrik B. Dukefoss, Wannan Tang, Klas H. Pettersen, Daniel M. Bjoernstad, P. Johannes Helm, Vidar Jensen, Rolf Sprengel , KoenVervaeke Ole P. Ottersen, and Erlend A. Nagelhus (2017), Deletion of Aquaporin-4 Curtails Extracellular Glutamate Elevation in Cortical Spreading Depression in Awake Mice, Cerebral Cortex 27(1):24-33

Published Apr. 13, 2011 10:59 AM - Last modified Dec. 18, 2019 2:29 PM

Research groups