Our research focuses on neuron-glial interactions in the intact brain
Our vision is to understand the cross-talk between neurons and glia in the intact brain under physiological conditions, and advance our knowledge on neuronal-glial interactions in neurological diseases. To monitor cell function in the intact brain we will use two-photon microscopy and genetically encoded fluorescent sensors. Optogenetic approaches will be used to manipulate the function of individual cells and networks. To avoid the confounding effects of anesthesia, experiments will be carried out in awake behaving animals. Our goal is thus to establish protocols for simultaneous all-optical manipulation and recording of neuon-glia circuit activity in awake behaving animals. This strategy is expected to give fundamentally new insight on neuron-glia circuit function in health and disease, and may pave the way for development of new diagnostic tools and treatments.
Our combined areas of expertise spans in vivo and in vitro electrophysiology, animal behavior analysis, two-photon microscopy, molecular neurobiology, neuroanatomy/electron microscopy, neurology and translational medicine. We have a wide national and international network of scientists with expertise in protein structure and engineering, optogenetics, optical imaging, and animal disease models