Arne Klungeland, Centre for Embryology and Healthy Development, UiO
Methylating and demethylating DNA and RNA: analogy of repair and regulation
The discovery of methylated DNA, as well as the pletora of modifications on RNA, were described many years ago. Their reversible potential, and the discovery of enzymes required for this, are much more recent discoveries. This has led to a tremendeous research interest in epigenetic and epitranscriptomic regulation and their key role in meiosis and the beginning of life In the preimplantation embryo. The presentation will also discuss the similarity and overlap between families of proteins and enzymes for DNA repair and regulation.
Vipin Kumar, Mathelier group, NCMM, UiO
Dissecting RNA-chromatin interactions from RADICL-data
Beyond RNA's role as messenger between DNA information and protein production, there is a vast literature reporting its diversity and its involvement in a variety of nuclear processes. Comprehensively charting the complex landscape of RNAs' activity in terms of their footprint along the chromatin would constitute an important step in our understanding of their multi-faceted function. In this presentation I will introduce our work on RADICL-seq, a high-throughput sequencing technology that probes genome-wide the chromatin interaction from the transcriptome. I will present preliminary results illustrating how the RNA seems to aggregate nucleus-wide in a cell-type specific manner, in line with previously reported RNA condensates. I will further describe how we characterised the possible functional implications of these RNA aggregation events.