Guest lecture: De novo generation of diverse organ buds from stem cells
The Sullivan group at the Institute of Basic Medical Sciences welcomes all interested to a guest lecture given by Takanori Takebe, leading expert on organ regeneration.
In vitro organogenesis is now becoming a realistic goal of stem cell biology; however, one practical challenge is to develop a four-dimensional (4-D) stem cell culture system whereby multiple progenitors communicate in a spatiotemporal manner, akin to organogenesis (Clin Pharmacol Ther, 2014). During early hepatogenesis, multicellular communications occur between mesenchymal stem cells, undifferentiated vascular endothelial cells and anterior visceral endodermal cells to initiate the budding of the rudimentary liver in the foregut. To recapitulate early organogenesis, we recently showed that specified hepatic cells self-organized into 3-D iPSC-derived liver buds when co-cultivated with multiple stromal cell populations. By transplanting in vitro grown organ buds, we have demonstrated the vascularized and functional liver tissues in an immunodeficient animal with therapeutic potential (Nature, 2013 & Nature Protocols, 2014). Furthermore, this approach is applicable to other systems by delineating the mechanisms guiding organ bud formation (J Clin Invest, 2014 & Cell Stem Cell, 2015). In this talk, I will summarize the state-of-art of these organ bud based approaches, and discuss their future potential applications.
Takanori Takebe received his M.D. in 2011 from Yokohama City University School of Medicine, Japan after a few years training as a research associate at the Scripps Research Institute and as an external medical student at Columbia University Medical Center in the US.
After receiving his M.D., he worked as a research associate at the same university. He became a project leader in 2012 at Yokohama City University Advanced Medical Research and later in 2013 appointed an associate professor position at Yokohama City University. He now has a joint position as an assistant professor at Cincinnati Children’s Hospital Medical Center and a visiting associate professor at Stanford University in the US.
He has published a number of pioneering works on organ regeneration from iPS cells.