IMB Distinguished Seminar: Upscaling human pluripotent stem cell culture and cardiac differentiation in suspension
We are happy to announce the third IMB Distiguished Seminar, where Dr Robert Zweigerdt of Hannover Medical School will give the lecture "Upscaling human pluripotent stem cell culture and cardiac differentiation in suspension".
About the lecturer
Dr Robert Zweigerdt
- Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO)
- REBIRTH Custer of Excellence (From REgenerative BIology to Recontructive THerapy)
- Hannover Medical School, Germany
Robert Zweigerdt´s research has resulted in recent milestone papers on the defined, single cell inoculated cultivation of human pluripotent stem cells (hPSC, including hESCs and hiPSCs) as “cells only” aggregates in suspension culture, independent of micro-carriers or other extracellular matrices. This technique enables straightforward up-scaling of hPSC culture in fully controllable stirred-tank bioreactors providing the required “raw material” for the mass production of any differentiated human PSC-derived progeny. Through this approach, his laboratory is generating pure human hPSC-derived cardiomyocytes (CMs) in chemically defined, clinically compliant culture media in suspension culture. He is currently working on combining the hPSC suspension culture process with differentiation protocols in controlled bioreactors to develop clinically relevant conditions for cardiomyocyte production, with the aim of using these in cardiac tissue engineering and in vitro modelling of cardiomyopathies.