IMB Distinguished Seminar: 3D Genome Organization and Gene Transcription Regulation
The iCAM research group at IMB is happy to announce a guest lecture given by professor Yijun Ruan of The Jackson Laboratory for Genomic Medicine.
The linear form of DNA sequences in a human genome is about 2 meters long, which has to be folded in micrometer-sized nuclear space for genome functions. Although most of our current understandings of the human genome functions are based on linear explanations, it has been speculated that the 3-dimension (3D) chromatin and high-order genome organization mediated by protein factors must play important roles in shaping the mechanisms of nuclear process such as transcription regulation and DNA replication. Advanced DNA sequencing and chromatin immunoprecipitation (ChIP) have allowed the development of high-throughput technologies for mapping genome-wide chromatin interactions such as ChIA-PET. Our recent results suggest that CTCF-mediated chromatin interaction serve as structural foci for spatial organization of constitutive genes concordant with CTCF-motif orientation, whereas RNAPII interacts within these structures by selectively drawing cell-type-specific genes towards CTCF-foci for coordinated transcription. We also show that haplotype-variants and allelic-interactions have differential effects on chromosome configuration influencing gene expression and may provide mechanistic insights into functions associated with disease susceptibility. Further 3D-genome strategy will provide novel insights in the topological mechanism of human variations and diseases
About the lecturer
Dr. Yijun Ruan is the Florine Roux Family Endowed Chair Professor and Director for Genomic Sciences at The Jackson Laboratory for Genomic Medicine. Prior to joining JAX, he was one of the founding members of the Genome Institute of Singapore, and served as a senior group leader and associate director for genome technology from 2002 to 2012. He played pivotal roles in establishing Singapore’s genomics capability and the award winning genomics programs. His primary research interest is to elucidate 3D genome organization and underlining regulatory functions in human and model organisms, with a focus to uncover the complex mechanisms of gene transcription regulation. His strategies include the development of innovative genome interrogation technologies to address questions pertinent to human diseases.