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Metoder

Vårt laboratorium, noen ganger i samarbeid med andre, har introdusert følgende metoder til Norge og Skandinavia:

  • Single-electrode voltage clamp recording from brain neurons (1986),
  • Patch clamp recording from brain neurons in vitro (1988) and in vivo (2012).
  • Two-photon (‘multi-photon’) laser microscopy (collaboration with P.J. Helm and others, 2000-2015)
  • Compartmental computational and mathematical models of brain neurons (1997).
  • Patch clamp recording from dendrites (2004)
  • Dynamic clamp, i.e. coupling a computer model to a live neuron in real time, to mimic, add or cancel intrinsic conductances in neurons (2005).  
  • Predictive computational modelling, i.e. extracting specific, unexpected predictions from computer simulations followed by experimental testing (2006).  
  • Dual and trippel simultaneous patch clamp recordings from dendrites, soma and axon with 2-3 electrodes in whole-cell configuration on the same neuron (2009)
  • Real-time laser scanning microscopy with Nipkow disk (2010)
  • Patch clamp recording from axons: axon terminals (2011), and axon blebs (2012).
  • nTMS-hdEEG (navigated Transcranial Magnetic Stimulation, combined with high-density ElectroEncephaloGrapy) in humans, for measuring the Perturbational Complexity Index (PCI) that is used for objective assessment of conscuousness (2015).

 

Andre metoder som brukes i vårt laboratorium:

  • Optical photolysis (“uncageing”) of “caged” compounds (glutamate, cyclic AMP, calcium chelators, etc.)
  • Genetically engineered viral vectors for targeted genetic manipulation of neurons (AAV, ; produced in our lab).
  • Transgenic mice (produced elsewhere)
  • Optogenetic manipulation of neurons (ChR2)
  • Sharp-electrode intracellular recordings
  • Local Field Potential (LFP) extracellular recordings
  • Behavioral analysis (open field, water maze, cross maze, rotarod etc.)
Publisert 17. des. 2018 16:06 - Sist endret 17. des. 2018 16:07