Paul Johannes Helm

Senioringeniør - Gliaceller
Bilde av Paul Johannes Helm
English version of this page
Telefon +47 22851159
Mobiltelefon 412 42 733
Rom 0347
Brukernavn
Besøksadresse Sognsvannsveien 9 Domus Medica 0372 OSLO
Postadresse Postboks 1103 Blindern 0317 OSLO

Bakgrunn

1988: Dipl. Phys. i Kjernefysikk fra Johannes-Gutenberg Universitetet i Mainz, Tyskland

1994: Tekn. lic. i Teknisk Fysikk fra KTH i Stockholm, Sverige

1997: Tekn. dr. i Teknisk Fysikk fra KTH i Stockholm, Sverige

Arbeidsoppgaver

  • Design og installasjon av Optisk Mikroskopi, spesielt konfokalt og ikke-lineært lasermikroskopi
  • Utdannelse av brukere

Publikasjoner

U. Dinger, S. Dutta, P.J. Helm, G. Huber, T. Kühl (1988), Laser Sättigungsspektroskopie am Ag-I-Atomstrahl, Verhandlungen der deutschen physikalischen Gesellschaft, Reihe VI, Bd.23, p.14

Paul Johannes Helm,Sättigungsspektroskopische Untersuchungen an den Hüllenspektren der stabilen Silberisotope 107Ag und 109Ag, Diploma Thesis, Johannes Gutenberg-University, Mainz, FRG, 1988

B. Ulfhake, K. Carlsson, K. Mossberg, U. Arvidsson and P. J. Helm (1991), Imaging of fluorescent neurons labelled with fluoro-gold and fluorescent axon terminals labelled with AMCA (7-amino-4-methylcoumarine-3-acetic acid) conjugated antiserum using a UV-laser confocal scanning microscope, Journal of Neuroscience Methods, Vol. 40, pp. 39-48

N. Burnashev, A. Khodorova, P. Jonas, P.J. Helm, W. Wisden, H. Monyer, P.H. Seeburg, and B. Sakmann (1992), Calcium Permeable AMPA-Kainate Receptors in Fusiform Cerebellar GlialCells, Science, Vol. 256, pp. 1566-1570

Carlsson, Kjell; Mossberg, Karin; Helm, Paul J.; Philip, Johan (1992); Use of UV-excitation for confocal fluorescence microscopy in a conventional beam-scanning instrument, Proc. SPIE Vol. 1660, p. 466-481, Biomedical Image Processing and Three-Dimensional Microscopy, Raj S. Acharya; Carol J. Cogswell; Dmitry B. Goldgof; Eds.

K. Carlsson, K. Mossberg, P.J. Helm, and J.Philip (1992), Use of UV Excitation in Confocal Laser Scanning Fluorescence Microscopy, Micron and Microscopica Acta, Vol. 23, No. 4, pp. 413-428

N. Melamed, P.J. Helm, and R. Rahamimoff (1993), Confocal Microscopy Reveals Coordinated Calcium Fluctuations and Oscillations in Synaptic Boutons, The Journal of Neuroscience, Vol.13, No.2, pp. 632-649

P.J. Helm,A confocal scanning laser microscope for quantitative measurements of the intracellular dynamics of Ca2+ ions in living biological cells,Thesis accepted by the Royal Institute of Technology, Stockholm, Sweden,as Licentiatsavhandling for the academic degree of a "TeknologieLicentiat (KTH)".TRITA-FYS-4038, Stockholm, August 1994

P.J. Helm, O. Franksson, and K. Carlsson (1995), A confocal scanning laser microscope for quantitative ratiometric 3D measurements of [Ca2+] and Ca2+ diffusions in living cells stained with Fura-2, Pflügers Archiv - European Journal of Physiology, Vol. 429, pp. 672-681

H. Markram, P.J. Helm, and B. Sakmann (1995), Dendritic calcium transients evoked by single back-propagating action potentials in rat neocortical pyramidal neurons, Journal of Physiology, Vol. 485, pp. 1-20

P.J. Helm (1996), A microscopic setup for combined, and time coordinated electrophysiological and confocal fluorescence microscopic experiments on neurons in living brain slices, Review of Scientific Instruments, Vol. 67, No.2, Feb. 1996, pp. 530-534

Ardan Patwardhan and Paul Johannes Helm (1996), A 3-D point measurement CSLM system for the study of time dynamic phenomena, TRITA-FYS 4041, ISRN/KTH/FYS/R--4041--SE

PJ Helm (1997), Dishes for confocal scanning laser microscopy on calibration solutions and living, cultured cells, TRITA-FYS 4043, ISRN/KTH/FYS/R--4043--SE

PJ Helm (1997), A confocal scanning laser microscope for cell physiological research, Thesis accepted by the Royal Institute of Technology, Stockholm, Sweden, as Doktorsavhandling for the academic degree of a "Teknologie Doktor (KTH)", i.e. PhD in Engineering Sciences. TRITA-FYS 4044, ISRN/KTH/FYS/R--4044--SE

PJ Helm, A Patwardhan, and EMM Manders (1997), A study of the precision of confocal, ratiometric, Fura-2 based [Ca2+] measurements, Cell Calcium 22(4):287-298

Linda Bergersen, Ola Wærhaug, Johannes Helm, Marion Thomas, PetterLaake, Andrew J. Davies, Mariangela C. Wilson, Andrew P. Halestrap, Ole P. Ottersen (2001), A novel postsynaptic density protein: the monocarboxylate transporterMCT2 is co-localized with δ-glutamate receptors in postsynaptic densities of parallel fiber-Purkiň cell synapses, Exp Brain Res 136:523-534

P. Johannes Helm, Finn-Mogens Haug, Johan F. Storm, Ole-Petter Ottersen (2001), Design and installation of a multi-mode microscopy system, Proc. SPIE, Vol. 4262:396-406

Gabriela Bezakova, Johannes P. Helm, Maura Francolini, and Terje Lømo (2001), Effects of Purified Recombinant Neural and Muscle Agrin on Skeletal Muscle Fibers In Vivo, The Journal of Cell Biology 153(7):1441-1452

Thomas M.J., Sjaastad I., Andersen K., Helm P.J., Wasserstrom J.A., Sejersted O.M. Ottersen O.P. (2003), Localization and function of the Na+/Ca2+-exchanger in normal and detubulated rat cardiomyocytes, Journal of Molecular & Cellular Cardiology. 35(11):1325-37

G. Nase, P.J. Helm, O.P.Ottersen (2005), Visualization of Structural Changes at the cellular and subcellular Level in the Cerebral Cortex of Live Mice, Program No. 189.9. 2005 Abstract Viewer/Itinerary Planner. Washington, DC: Society for Neuroscience, 2005.

G. Nase, P.J. Helm, O.P.Ottersen (2005), A multiphoton laser scanning microscope setup for transcranial in vivo brain imaging on mice, Review of Scientific Instruments 76:123702-1-123702-5.

Gabriele Nase, P.Johannes Helm, Ole Petter Ottersen (2006), Structural changes at the cellular and subcellular level in the cerebral cortex of mice visualized by means of trans-cranial multi photon in vivo microscopy, Proc. SPIE, 6089:608920

G. Nase, P.J. Helm, O.P. Ottersen (2006),In vivo measurements of cell volume respons to hypo-osmotic stress, Program No. 433.1/D71 2006 Abstract Viewer/Itinerary Planner. Atlanta, GA, Society for Neuroscience, 2006.

P.J. Helm, Ole P. Ottersen , G. Nase (2007), Optical effects of the cranium in trans-cranial in vivo two photon laser scanning microscopy in mice, Proc. SPIE, 6442:644221

P.J. Helm, Ole P. Ottersen , G. Nase (2007), An improved method for the analysis of some optical properties of the mouse cranium: implications for in vivo optical imaging, Program No. 533.3/HHH27 2007, Annual Meeting of the International Society for Neuroscience, San Diego, CA, USA, Oct. 31 - Nov. 7, 2007

P.J. Helm, N.F. Proske, F. Baur (2007), Teaching and Discussing Neuroscience in Pious Christian Circles, Program No. 27.17/OOO19 2007, Annual Meeting of the International Society for Neuroscience, San Diego, CA, USA, Oct. 31 - Nov. 7, 2007

G. Nase, P.J.Helm, T. Oguchi, L.N.G. Nilsson, L. Lannfelt, O.P. Ottersen, R. Torp (2008), Local impact of perivascular plaques on cerebral blood flow dynamics in a transgenic mouse model of Alzheimer's disease, Proc. SPIE, 6860:686022

G. Nase, P.J. Helm, R. Enger & O. P. Ottersen (2008), Water Entry into Astrocytes during Brain Edema Formation, Glia 56:895-902

G. Nase, P. J. Helm, L. Nilsson, L. Lannfelt, O.P. Ottersen, R. Torp (2008), Astrocyte morphological dynamics in a mouse model of Alzheimer's disease with the Arctic and Swedish mutation, Program No. 637.25/J8, Annual Meeting of the International Society for Neuroscience, Washington, DC, USA, Nov. 15-19, 2008

P. Johannes Helm, Ole P. Ottersen, Gabriele Nase (2009), Analysis of optical properties of the mouse cranium - Implications for in vivo multi photon laser scanning microscopy, Journal of Neuroscience Methods 178:316-322

P. J. Helm, T. Reppen, P. Heggelund (2009), A setup for combined multi photon laser scanning microscopic and multi electrode patch clamp experiments on brain slices, Proc. SPIE 7183:71832Q-1 - 71832Q-8

P. Johannes Helm, Gabriele Nase, Paul Heggelund and Trond Reppen (2010), Complementary equipment for controlling multiple laser beams on single scanner MPLSM systems, Proc. SPIE 7569, 75692Y

P. Johannes Helm and Ole P. Ottersen (2011), Proposal of a new method to measure FRET quantitatively in living or fixed biomedical specimens on a laser microscope, Proc. SPIE 7903, 790331

P. Johannes Helm, Sigita Augustinaite, Trond Reppen and Paul Heggelund (2011), Simultaneous Chemical Activation and Image Scanning on Single Scanner Laser Scanning Microscope Systems, Program no. 519.25, Annual Meeting of The International Society for Neuroscience, Washington, DC, USA, Nov. 12-16, 2011

P. Johannes Helm, N. Frank Proske and Friedbert Baur (2011), Faith beyond the Neuron, Program no. 28.20SA, Annual Meeting of the International Society for Neuroscience, Washington, DC, USA, Nov. 12-16, 2011

P. Johannes Helm (2012), Recommendations for the design and the installation of large laser scanning microscopy systems, Proc. SPIE, 8226, 82262M-82262M-7

Helm P.J. (2012), Proposal of a New Method for Measuring Foerster Resonance Energy Transfer (FRET) Rapidly, Quantitatively and Non-Destructively, International Journal of Molecular Sciences 13(10):12367-12382

Xi Wu, Ji-Yao Chen, Andreas Brech, Caihong Fang, Jianfang Wang, P. Johannes Helm, Qian Peng (2013), The use of femto-second lasers to trigger powerful explosions of gold nanorods to destroy cancer cells, Biomaterials 34:6157-6162

Sigita Augustinaite,Bernd Kuhn, Paul Johannes Helm, and Paul Heggelund (2014), NMDA Spike/Plateau Potentials in Dendrites of Thalamocortical Neurons, The Journal of Neuroscience 34(33):10892-10905

Wannan Tang, Karolina Szokol, Vidar Jensen, Rune Enger, Chintan A. Trivedi, Øivind Hvalby, P. Johannes Helm, Loren L. Looger, Rolf Sprengel, and Erlend A. Nagelhus (2015), Stimulation-Evoked Ca2+ Signals in Astrocytic Processes at Hippocampal CA3-CA1 Synapses of Adult Mice Are Modulated by Glutamate and ATP, The Journal of Neuroscience 35(7):3016-3021

Rune Enger, Wannan Tang, Gry Fluge Vindedal, Vidar Jensen, P. Johannes Helm, Rolf Sprengel ,Loren L. Looger , and Erlend A. Nagelhus (2015), Dynamics of Ionic Shifts in Cortical Spreading Depression, Cerebral Cortex 25(11):4469-76

Rune Enger, Didrik B. Dukefoss, Wannan Tang, Klas H. Pettersen, Daniel M. Bjoernstad, P. Johannes Helm, Vidar Jensen, Rolf Sprengel , KoenVervaeke Ole P. Ottersen, and Erlend A. Nagelhus (2017), Deletion of Aquaporin-4 Curtails Extracellular Glutamate Elevation in Cortical Spreading Depression in Awake Mice, Cerebral Cortex 27(1):24-33

Publikasjoner

  • Enger, Rune; Dukefoss, Didrik Bakke; Tang, Wannan; Pettersen, Klas; Bjørnstad, Daniel Marelius; Helm, Paul Johannes; Jensen, Vidar; Sprengel, Rolf; Vervaeke, Koen Gerard Alois; Ottersen, Ole Petter & Nagelhus, Erlend Arnulf (2016). Deletion of aquaporin-4 curtails extracellular glutamate elevation in cortical spreading depression in awake mice. Cerebral Cortex.  ISSN 1047-3211. . doi: 10.1093/cercor/bhw359
  • Enger, Rune; Tang, Wannan; Vindedal, Gry Fluge; Jensen, Vidar; Helm, Paul Johannes; Sprengel, Rolf; Looger, Loren L. & Nagelhus, Erlend Arnulf (2015). Dynamics of ionic shifts in cortical spreading depression. Cerebral Cortex.  ISSN 1047-3211.  25(11), s 4469- 4476 . doi: 10.1093/cercor/bhv054 Vis sammendrag
  • Tang, Wannan; Szokol, Karolina; Jensen, Vidar; Enger, Rune; Trivedi, Chintan A.; Hvalby, Øyvind Christian dødsbo; Helm, Paul Johannes; Looger, Loren L.; Sprengel, Rolf & Nagelhus, Erlend Arnulf (2015). Stimulation-evoked Ca2+ signals in astrocytic processes at hippocampal CA3-CA1 synapses of adult mice are modulated by glutamate and ATP. Journal of Neuroscience.  ISSN 0270-6474.  35(7), s 3016- 3021 . doi: 10.1523/JNEUROSCI.3319-14.2015 Vis sammendrag
  • Augustinaite, Sigita; Kuhn, Bernd; Helm, Paul Johannes & Heggelund, Paul (2014). NMDA spike/plateau potentials in dendrites of thalamocortical neurons. Journal of Neuroscience.  ISSN 0270-6474.  34(33), s 10892- 10905 . doi: 10.1523/JNEUROSCI.1205-13.2014
  • Wu, Xi; Chen, Ji-Yao; Brech, Andreas; Fang, Caihong; Wang, Jianfang; Helm, Paul Johannes & Qian, Peng (2013). The use of femto-second lasers to trigger powerful explosions of gold nanorods to destroy cancer cells. Biomaterials.  ISSN 0142-9612.  34(26), s 6157- 6162 . doi: 10.1016/j.biomaterials.2013.04.048
  • Helm, Paul Johannes (2012). Proposal of a New Method for Measuring Forster Resonance Energy Transfer (FRET) Rapidly, Quantitatively and Non-Destructively. International Journal of Molecular Sciences.  ISSN 1422-0067.  13(10), s 12367- 12382 . doi: 10.3390/ijms131012367 Fulltekst i vitenarkiv. Vis sammendrag
  • Helm, Paul Johannes (2012). Recommendations for the design and the installation of large laser scanning microscopy systems. Proceedings of SPIE, the International Society for Optical Engineering.  ISSN 0277-786X.  8226, s 82262M- 82262M-7 . doi: 10.1117/12.906040 Vis sammendrag
  • Helm, Paul Johannes & Ottersen, Ole Petter (2011). Proposal of a new method to measure FRET quantitatively in living or fixed biomedical specimens on a laser microscope. Proceedings of SPIE, the International Society for Optical Engineering.  ISSN 0277-786X.  7903, s 790331-1- 790331-13 . doi: 10.1117/12.874073 Fulltekst i vitenarkiv. Vis sammendrag
  • Helm, Paul Johannes; Ottersen, Ole Petter & Nase, Gabriele (2009). Analysis of optical properties of the mouse cranium-Implications for in vivo multi photon laser scanning microscopy. Journal of Neuroscience Methods.  ISSN 0165-0270.  178(2), s 316- 322 . doi: 10.1016/j.jneumeth.2008.12.032
  • Nase, Gabriele; Helm, Paul Johannes; Oguchi, Tomohiro; Nilsson, L. N. G.; Lannfelt, L.; Ottersen, Ole Petter & Torp, Reidun (2008). Local impact of perivascular plaques on cerebral blood flow dynamics in a transgenic mouse model of Alzheimer's disease. Proceedings of SPIE, the International Society for Optical Engineering.  ISSN 0277-786X.  6860 . doi: 10.1117/12.762739 Fulltekst i vitenarkiv.
  • Nase, Gabriele; Helm, Paul Johannes; Enger, Rune & Ottersen, Ole Petter (2008). Water entry into astrocytes during brain edema formation. Glia.  ISSN 0894-1491.  56, s 895- 902 . doi: 10.1002/glia.20664
  • Helm, Paul Johannes; Ottersen, Ole Petter & Nase, Gabriele (2007). optical effects of the cranium in trans-cranial in vivo two photon laser scanning microscopy in mice. Proceedings of SPIE, the International Society for Optical Engineering.  ISSN 0277-786X.
  • Nase, Gabriele; Helm, Paul Johannes; Reppen, Trond & Ottersen, Ole Petter (2005). A multi photon laser scanning microscope setup for trans-cranial in vivo brain imaging on mice. Review of Scientific Instruments.  ISSN 0034-6748.  76, s 123702-1- 123702-5
  • Thomas, Marion J.; Sjaastad, Ivar; Andersen, K; Helm, Paul Johannes; Wasserstrom, J. A.; Sejersted, Ole M & Ottersen, Ole Petter (2003). Localization and function of the Na(+)/Ca(2+)-exchanger in normal and detubulated rat cardiomycocytes. Journal of Molecular and Cellular Cardiology.  ISSN 0022-2828.  35(11), s 1325- 1337
  • Ottersen, Ole Petter & Helm, Paul Johannes (2002). Neurobiology: How hardwired is the brain?. Nature.  ISSN 0028-0836.  420(6719), s 751- 752
  • Bezakova, Gabriela; Helm, Paul Johannes; Francolini, M. & Lømo, Terje (2001). Effects of purified recombinant neural and muscle agrin on skeletal muscle fibers in vivo. Journal of Cell Biology.  ISSN 0021-9525.  153, s 1441- 1452 Vis sammendrag
  • Bezakova, Gabriela; Helm, Paul Johannes; Francolini, Maura & Lømo, Terje (2001). Effects of purified recombinant neural and muscle agrin on skeletal muscle fibers in vivo. Journal of Cell Biology.  ISSN 0021-9525.  153(7), s 1441- 1452
  • Helm, Paul Johannes; Haug, Finn-Mogens Smejda; Storm, Johan Fredrik & Ottersen, Ole Petter (2001). Design and installation of a multi-mode microscopy system. International Society for Optical Engineering Proceedings Series.  ISSN 1017-2653.  4262
  • Helm, Paul Johannes; Haug, Finn-Mogens Smejda; Storm, Johan Fredrik & Ottersen, Ole Petter (2001). Design and installation of a multi-mode microscopy system. International Society for Optical Engineering Proceedings Series.  ISSN 1017-2653.  4262

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  • Augustinaite, Sigita; Kuhn, B; Helm, Paul Johannes & Heggelund, Paul (2013). NMDA spikes in dendrites of thalamocortical neurons. Abstracts - Society for Neuroscience.  ISSN 0190-5295.  43
  • Helm, Paul Johannes; Augustinaite, Sigita & Heggelund, Paul (2011). Simultaneous chemical activation and image scanning on single scanner laser scanning microscope systems. Abstracts - Society for Neuroscience.  ISSN 0190-5295.  41
  • Helm, Paul Johannes; Nase, Gabriele; Heggelund, Paul & Reppen, Trond (2010). Complementary equipment for controlling multiple laser beams on single scanner MPLSM systems. Proceedings of SPIE, the International Society for Optical Engineering.  ISSN 0277-786X.  7569
  • Helm, Paul Johannes; Reppen, Trond & Heggelund, Paul (2009). A setup for combined multiphoton laser scanning miscroscopic and multi-electrode patch clamp experiments on brain slices. Proceedings of SPIE, the International Society for Optical Engineering.  ISSN 0277-786X.  7183, s 7183Q-1- 7183X-8

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Publisert 13. apr. 2011 11:03 - Sist endret 18. des. 2019 07:52

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