Arne Klungland

• Grøsvik, Kristin; Tesfahun, Almaz Nigatu; Muruzábal-Lecumberri, Izaskun; Haugland, Gyri Teien; Leiros, Ingar; Ruoff, Peter; Kvaløy, Jan Terje; Knævelsrud, Ingeborg; Ånensen, Hilde; Alexeeva, Marina; Sato, Kousuke; Matsuda, Akira; Alseth, Ingrun; Klungland, Arne & Bjelland, Svein (2020). The Escherichia coli alkA Gene Is Activated to Alleviate Mutagenesis by an Oxidized Deoxynucleoside. Frontiers in Microbiology.  ISSN 1664-302X.  11 . doi: 10.3389/fmicb.2020.00263 Fulltekst i vitenarkiv.
• Kong, Xiang Yi; Vik, Erik Sebastian; Nawaz, Meh Sameen; Berges, Natalia; Dahl, Tuva Børresdatter; Vågbø, Cathrine; Suganthan, Rajikala; Segers, Filip; Holm, Sverre; Quiles-Jiménez, Ana M T; Gregersen, Ida; Fladeby, Cathrine; Aukrust, Pål; Bjørås, Magnar; Klungland, Arne; Halvorsen, Bente & Alseth, Ingrun (2020). Deletion of Endonuclease V suppresses chemically induced hepatocellular carcinoma. Nucleic Acids Research (NAR).  ISSN 0305-1048.  48(8), s 4463- 4479 . doi: 10.1093/nar/gkaa115 Fulltekst i vitenarkiv.
• Li, Miaomiao & Klungland, Arne (2020). Modifications and interactions at the R-loop. DNA Repair.  ISSN 1568-7864.  96 . doi: 10.1016/j.dnarep.2020.102958
• Sankar, Aditya; Lerdrup, Mads; Manaf, Adeel; Johansen, Jens Vilstrup; Gonzalez, Javier Martin; Borup, Rehannah; Blanshard, Robert; Klungland, Arne; Hansen, Klaus; Andersen, Claus Yding; Dahl, John Arne; Helin, Kristian & Hoffmann, Eva R. (2020). KDM4A regulates the maternal-to-zygotic transition by protecting broad H3K4me3 domains from H3K9me3 invasion in oocytes. Nature Cell Biology.  ISSN 1465-7392.  22(4), s 380- 388 . doi: 10.1038/s41556-020-0494-z
• Yang, Mingyi; Lin, Xiaolin; Segers, Filip; Suganthan, Rajikala; Hildrestrand, Gunn Annette; Rinholm, Johanne Egge; Aas, Per Arne; Sousa, Mirta; Holm, Sverre; Bolstad, Nils; Warren, David; Berge, Rolf Kristian; Johansen, Rune Forstrøm; Yndestad, Arne; Kristiansen, Elise; Klungland, Arne; Gomez, Luisa Fernanda Luna; Eide, Lars; Halvorsen, Bente; Aukrust, Pål & Bjørås, Magnar (2020). OXR1A, a Coactivator of PRMT5 Regulating Histone Arginine Methylation. Cell reports.  ISSN 2211-1247.  30(12), s 4165- 4178 . doi: 10.1016/j.celrep.2020.02.063 Fulltekst i vitenarkiv.
• Abakir, Abdulkadir; Giles, Tom C.; Cristini, Agnese; Foster, Jeremy M.; Dai, Nan; Starczak, Marta; Rubio-Roldan, Alejandro; Li, Miaomiao; Eleftheriou, Maria; Crutchley, James; Flatt, Luke; Young, Lorraine; Gaffney, Daniel J.; Denning, Chris; Dalhus, Bjørn; Emes, Richard D.; Gackowski, Daniel; Corrêa, Ivan R., Jr.; Garcia-Perez, Jose L.; Klungland, Arne; Gromak, Natalia & Ruzov, Alexey (2019). N6-methyladenosine regulates the stability of RNA:DNA hybrids in human cells.. Nature Genetics.  ISSN 1061-4036.  52(1), s 48- 55 . doi: 10.1038/s41588-019-0549-x
• Jarøy, Emilie Grimnes; Acosta Jiménez, Lourdes Patricia; Hotta, Ryo; Goldstein, Allan M.; Emblem, Ragnhild; Klungland, Arne & Ougland, Rune (2019). "Too much guts and not enough brains": (epi)genetic mechanisms and future therapies of Hirschsprung disease - a review. Clinical Epigenetics.  ISSN 1868-7075.  11 . doi: 10.1186/s13148-019-0718-x Fulltekst i vitenarkiv.
• Pilžys, Tomaš; Marcinkowski, Michał; Kukwa, Wojciech; Garbicz, Damian; Dylewska, Małgorzata; Ferenc, Karolina; Mieczkowski, Adam; Kukwa, Andrzej; Migacz, Ewa; Wołosz, Dominika; Mielecki, Damian; Klungland, Arne; Piwowarski, Jan; Poznański, Jarosław & Grzesiuk, Elżbieta (2019). ALKBH overexpression in head and neck cancer: potential target for novel anticancer therapy. Scientific Reports.  ISSN 2045-2322.  9(1) . doi: 10.1038/s41598-019-49550-x
• Prikrylova, Terezia; Robertson, Julia; Ferrucci, Francesca; Konorska, Dorota Joanna; Aanes, Håvard; Manaf, Adeel; Zhang, Beibei; Vågbø, Cathrine Broberg; Kusnierczyk, Anna; Gilljam, Karin Margaretha; Løvkvam-Køster, Maiken Caroline; Otterlei, Marit; Dahl, John Arne; Enserink, Jorrit; Klungland, Arne & Robertson, Adam Brian (2019). 5-hydroxymethylcytosine marks mammalian origins acting as a barrier to replication. Scientific Reports.  ISSN 2045-2322.  9 . doi: 10.1038/s41598-019-47528-3 Fulltekst i vitenarkiv.
• Zhang, Zhichao; Manaf, Adeel; Li, Yanjiao; Pérez, Sonia Peña; Suganthan, Rajikala; Dahl, John Arne; Bjørås, Magnar & Klungland, Arne (2019). Histone methylations define neural stem/progenitor cell subtypes in the mouse subventricular zone. Molecular Neurobiology.  ISSN 0893-7648. . doi: 10.1007/s12035-019-01777-5 Fulltekst i vitenarkiv.
• Alexeeva, Marina; Guragain, Prashanna; Tesfahun, Almaz Nigatu; Tomkuvienė, Miglė; Arshad, Aysha; Gerasimaitė, Rūta; Rukšėnaitė, Audronė; Urbanavičiūtė, Giedrė; Bjørås, Magnar; Lærdahl, Jon Kristen; Klungland, Arne; Klimašauskas, Saulius & Bjelland, Svein (2018). Excision of the doubly methylated base N4,5-dimethylcytosine from DNA by Escherichia coli Nei and Fpg proteins. Philosophical Transactions of the Royal Society of London. Biological Sciences.  ISSN 0962-8436.  373(1748) . doi: 10.1098/rstb.2017.0337 Fulltekst i vitenarkiv.
• Alexeeva, Marina; Moen, Marivi Nabong; Grøsvik, Kristin; Tesfahun, Almaz Nigatu; Xu, Xiang Ming; Muruzábal-Lecumberri, Izaskun; Olsen, Kristine Marie; Rasmussen, Anette; Ruoff, Peter; Kirpekar, Finn; Klungland, Arne & Bjelland, Svein (2018). Excision of uracil from DNA by hSMUG1 includes strand incision and processing. Nucleic Acids Research (NAR).  ISSN 0305-1048.  47(2), s 779- 793 . doi: 10.1093/nar/gky1184 Fulltekst i vitenarkiv.
• Askeland, Georgina; Dosoudilova, Zaneta; Rodinova, Marie; Klempir, Jiri; Liskova, Irena; Kusnierczyk, Anna; Bjørås, Magnar; Nesse, Gaute; Klungland, Arne; Hansikova, Hana & Eide, Lars (2018). Increased nuclear DNA damage precedes mitochondrial dysfunction in peripheral blood mononuclear cells from Huntington's disease patients. Scientific Reports.  ISSN 2045-2322.  8(1) . doi: 10.1038/s41598-018-27985-y Fulltekst i vitenarkiv.
• Askeland, Georgina; Rodinova, Marie; Štufková, Hana; Dosoudilova, Zaneta; Baxa, Monika; Smatlikova, Petra; Bohuslavova, Bozena; Klempir, Jiri; Nguyen, The Duong; Kuśnierczyk, Anna; Bjørås, Magnar; Klungland, Arne; Hansikova, Hana; Ellederova, Zdenka & Eide, Lars (2018). A transgenic minipig model of Huntington's disease shows early signs of behavioral and molecular pathologies. Disease Models and Mechanisms.  ISSN 1754-8403.  11(10) . doi: 10.1242/dmm.035949 Fulltekst i vitenarkiv.
• Li, Miaomiao; Zhao, Xu; Wang, Wei; Shi, Hailing; Pan, Qingfei; Lu, Zhike; Pérez, Sonia Peña; Suganthan, Rajikala; He, Chuan; Bjørås, Magnar & Klungland, Arne (2018). Ythdf2-mediated m6A mRNA clearance modulates neural development in mice. Genome Biology.  ISSN 1465-6906.  19(1) . doi: 10.1186/s13059-018-1436-y Fulltekst i vitenarkiv.
• Ma, Chunhui; Chang, Mengqi; Lv, Hongyi; Zhang, Zhi-Wei; Zhang, Weilong; He, Xue; Wu, Gaolang; Zhao, Shunli; Zhang, Yao; Wang, Di; Teng, Xufei; Liu, Chunying; Li, Qing; Klungland, Arne; Niu, Yamei; Song, Shuhui & Tong, Wei-Min (2018). RNA m6A methylation participates in regulation of postnatal development of the mouse cerebellum. Genome Biology.  ISSN 1465-6906.  19(1) . doi: 10.1186/s13059-018-1435-z Fulltekst i vitenarkiv.
• Wei, Jiangbo; Liu, Fange; Lu, Zhike; Fei, Qili; Ai, Yuxi; He, P. Cody; Shi, Hailing; Cui, Xiaolong; Su, Rui; Klungland, Arne; Jia, Guifang; Chen, Jianjung & He, Chuan (2018). Differential m6A, m6Am, and m1A Demethylation Mediated by FTO in the Cell Nucleus and Cytoplasm. Molecular Cell.  ISSN 1097-2765.  71(6), s 973- 985 . doi: 10.1016/j.molcel.2018.08.011
• Yaddehi Gamage, Thilini Hansamali Gamage; Gunnes, Gjermund; Lee, Robert Hugh; Louch, William Edward; Holmgren, Asbjørn; Bruton, Joseph D.; Lengle, Emma; Kolstad, Terje R Selnes; Revold, Tobias; Amundsen, Silja Svanstrøm; Dalen, Knut Tomas; Holme, Pål Andre; Tjønnfjord, Geir Erland; Christensen, Geir Arve; Westerblad, Håkan; Klungland, Arne; Bergmeier, Wolfgang; Misceo, Doriana & Frengen, Eirik (2018). STIM1 R304W causes muscle degeneration and impaired platelet activation in mice. Cell Calcium.  ISSN 0143-4160.  76, s 87- 100 . doi: 10.1016/j.ceca.2018.10.001 Fulltekst i vitenarkiv. Vis sammendrag

  STIM1 and ORAI1 regulate store-operated Ca2+ entry (SOCE) in most cell types, and mutations in these proteins have deleterious and diverse effects. We established a mouse line expressing the STIM1 R304 W gain-of-function mutation causing Stormorken syndrome to explore effects on organ and cell physiology. While STIM1 R304 W was lethal in the homozygous state, surviving mice presented with reduced growth, skeletal muscle degeneration, and reduced exercise endurance. Variable STIM1 expression levels between tissues directly impacted cellular SOCE capacity. In contrast to patients with Stormorken syndrome, STIM1 was downregulated in fibroblasts from Stim1R304W/R304W mice, which maintained SOCE despite constitutive protein activity. In studies using foetal liver chimeras, STIM1 protein was undetectable in homozygous megakaryocytes and platelets, resulting in impaired platelet activation and absent SOCE. These data indicate that downregulation of STIM1 R304 W effectively opposes the gain-of-function phenotype associated with this mutation, and highlight the importance of STIM1 in skeletal muscle development and integrity.

• Aas, Aleksander; Isakson, Pauline; Bindesbøll, Christian; Alemu, Endalkachew Ashenafi; Klungland, Arne & Simonsen, Anne (2017). Nucleocytoplasmic shuttling of FTO does not affect starvation-induced autophagy. PLOS ONE.  ISSN 1932-6203.  12(3) . doi: 10.1371/journal.pone.0168182 Fulltekst i vitenarkiv.
• Chymkowitch, Pierre; Nguéa P, Aurélie; Aanes, Håvard; Robertson, Joseph; Klungland, Arne & Enserink, Jorrit (2017). TORC1-dependent sumoylation of Rpc82 promotes RNA polymerase III assembly and activity. Proceedings of the National Academy of Sciences of the United States of America.  ISSN 0027-8424.  114(5), s 1039- 1044 . doi: 10.1073/pnas.1615093114
• Jordan, Jennifer J.; Chhim, Sophea; Margulies, Carrie M.; Allocca, Mariacarmela; Bronson, Roderick T.; Klungland, Arne; Samson, Leona D. & Fu, Dragony (2017). ALKBH7 drives a tissue and sex-specific necrotic cell death response following alkylation-induced damage. Cell Death & Disease.  ISSN 2041-4889.  8 . doi: 10.1038/cddis.2017.343
• Kalikstad, Betty; Kultima, Hanna Goransson; Andersstuen, Terese; Klungland, Arne & Isaksson, Anders (2017). Gene expression profiles in preterm infants on continuous long-term oxygen therapy suggest reduced oxidative stress-dependent signaling during hypoxia. Molecular Medicine Reports.  ISSN 1791-2997.  15(4), s 1513- 1526 . doi: 10.3892/mmr.2017.6185 Fulltekst i vitenarkiv.
• Landfors, Miriam; Johansen, Jostein; Aronsen, Jan Magnus; Vågbø, Cathrine Broberg; Doré, Louis C.; He, Chuan; Sjaastad, Ivar; Sætrom, Pål; Fedorcsák, Péter; Dahl, John Arne; Aanes, Håvard; Fusser, Markus & Klungland, Arne (2017). Genome-wide profiling of DNA 5-hydroxymethylcytosine during rat Sertoli cell maturation. Cell Discovery.  ISSN 2056-5968.  3 . doi: 10.1038/celldisc.2017.13 Fulltekst i vitenarkiv.
• Olsen, Maria Belland; Hildrestrand, Gunn Annette; Scheffler, Katja; Vinge, Leif Erik; Alfsnes, Katrine; Palibrk, Vuk; Wang, Junbai; Neurauter, Christine Gran; Gomez, Luisa Fernanda Luna; Johansen, Jostein; Øgaard, Jonas; Ohm, Ingrid Kristine; Slupphaug, Geir; Kusnierczyk, Anna; Fiane, Arnt E; Brorson, Sverre Henning; Zhang, Lili; Gullestad, Lars; Louch, William Edward; Iversen, Per Ole; Østlie, Ingunn; Klungland, Arne; Christensen, Geir Arve; Sjaastad, Ivar; Sætrom, Pål; Yndestad, Arne; Aukrust, Pål; Bjørås, Magnar & Finsen, Alexandra (2017). NEIL3-Dependent Regulation of Cardiac Fibroblast Proliferation Prevents Myocardial Rupture. Cell reports.  ISSN 2211-1247.  18, s 82- 92 . doi: 10.1016/j.celrep.2016.12.009 Fulltekst i vitenarkiv.
• Pawar, Tina; Bjørås, Magnar; Klungland, Arne & Eide, Lars (2017). Metabolism and DNA repair shape a specific modification pattern in mitochondrial DNA.. Mitochondrion (Amsterdam. Print).  ISSN 1567-7249.  40, s 16- 28 . doi: 10.1016/j.mito.2017.09.002 Fulltekst i vitenarkiv.
• Rolseth, Veslemøy; Gomez, Luisa Fernanda Luna; Olsen, Ann-Karin; Suganthan, Rajikala; Scheffler, Katja; Neurauter, Christine Gran; Esbensen, Qin Ying; Kusnierczyk, Anna; Hildrestrand, Gunn Annette; Graupner, Anne; Andersen, Jill Mari; Slupphaug, Geir; Klungland, Arne; Nilsen, Hilde & Bjørås, Magnar (2017). No cancer predisposition or increased spontaneous mutation frequencies in NEIL DNA glycosylases-deficient mice. Scientific Reports.  ISSN 2045-2322.  7 . doi: 10.1038/s41598-017-04472-4 Fulltekst i vitenarkiv.
• Seifermann, Marco; Ulges, Alexander; Bopp, Tobias; Melcea, Svetlana; Schäfer, Andrea; Oka, Sugako; Nakabeppu, Yusaku; Klungland, Arne; Niehrs, Christof & Epe, Bernd (2017). Role of the DNA repair glycosylase OGG1 in the activation of murine splenocytes. DNA Repair.  ISSN 1568-7864.  58, s 13- 20 . doi: 10.1016/j.dnarep.2017.08.005
• Tang, Chong; Klukovich, Rachel; Peng, Hongying; Wang, Zhuqing; Yu, Tian; Zhang, Ying; Zheng, Huili; Klungland, Arne & Yan, Wei (2017). ALKBH5-dependent m6A demethylation controls splicing and stability of long 3'-UTR mRNAs in male germ cells. Proceedings of the National Academy of Sciences of the United States of America.  ISSN 0027-8424.  115(2), s E325- E333 . doi: 10.1073/pnas.1717794115
• Klungland, Arne & Robertson, Adam Brian (2016). Oxidized C5-methyl cytosine bases in DNA: 5-Hydroxymethylcytosine; 5-formylcytosine; and 5-carboxycytosine. Free Radical Biology & Medicine.  ISSN 0891-5849.  107, s 62- 68 . doi: 10.1016/j.freeradbiomed.2016.11.038
• Alemu, Endalkachew Ashenafi; He, Chuan & Klungland, Arne (2016). ALKBHs-facilitated RNA modifications and de-modifications. DNA Repair.  ISSN 1568-7864.  44, s 87- 91 . doi: 10.1016/j.dnarep.2016.05.026
• Dahl, John Arne; Jung, Inkyung; Aanes, Håvard; Greggains, Gareth; Manaf, Adeel; Lerdrup, Mads; Li, Guoqiang; Kuan, Samantha; Li, Bin; Lee, Ah Young; Preissl, Sebastian; Jermstad, Ingunn; Haugen, Mads Haugland; Suganthan, Rajikala; Bjørås, Magnar; Hansen, Klaus; Dalen, Knut Tomas; Fedorcsák, Péter; Ren, Bing & Klungland, Arne (2016). Broad histone H3K4me3 domains in mouse oocytes modulate maternal-to-zygotic transition. Nature.  ISSN 0028-0836.  537(7621), s 548- 552 . doi: 10.1038/nature19360
• Klungland, Arne; Dahl, John Arne; Greggains, Gareth; Fedorcsák, Péter & Filipczyk, Adam (2016). Reversible RNA modifications in meiosis and pluripotency. Nature Methods.  ISSN 1548-7091.  14(1), s 18- 22 . doi: 10.1038/nmeth.4111
• Landfors, Miriam; Nakken, Sigve; Fusser, Markus; Dahl, John Arne; Klungland, Arne & Fedorcsák, Péter (2016). Sequencing of FTO and ALKBH5 in men undergoing infertility work-up identifies an infertility-associated variant and two missense mutations. Fertility and Sterility.  ISSN 0015-0282.  105(5), s 1170- 1179e5 . doi: 10.1016/j.fertnstert.2016.01.002
• Lauritzen, Knut Husø; Hasan-Olive, Md Mahdi; Regnell, Christine E; Kleppa, Liv; Scheibye Knudsen, Morten; Gjedde, Albert; Klungland, Arne; Bohr, Vilhelm A; Storm-Mathisen, Jon & Bergersen, Linda Hildegard (2016). A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain. Neurobiology of Aging.  ISSN 0197-4580.  48, s 34- 47 . doi: 10.1016/j.neurobiolaging.2016.08.005
• Liu, Fange; Clark, Wesley; Luo, Guanzheng; Wang, Xiaoyun; Fu, Ye; Wei, Jiangbo; Wang, Xiao; Hao, Ziyang; Dai, Qing; Zheng, Guanqun; Ma, Honghui; Han, Dali; Evans, Molly; Klungland, Arne; Pan, Tao & He, Chuan (2016). ALKBH1-Mediated tRNA Demethylation Regulates Translation. Cell.  ISSN 0092-8674.  167(3), s 816- 828 . doi: 10.1016/j.cell.2016.09.038
• Mi, Shuangli; Klungland, Arne & Yang, Yun-Gui (2016). Base-excision repair and beyond —A short summary attributed to scientific achievements of Tomas Lindahl, Nobel Prize Laureate in Chemistry 2015. Science China Life Sciences.  ISSN 1674-7305.  59(1), s 89- 92 . doi: 10.1007/s11427-015-4983-4
• Møllersen, Linda; Moldestad, Olve; Rowe, Alexander D.; Bjølgerud, Anja; Holm, Ingunn; Tveterås, Linda Augustin; Klungland, Arne & Retterstøl, Lars (2016). Effects of anthocyanins on CAG repeat instability and behaviour in Huntington's disease R6/1 mice. PLOS Currents.  ISSN 2157-3999.  8(HuntingtonDisease, 2016) . doi: 10.1371/currents.hd.58d04209ab6d5de0844db7ef5628ff67
• Ougland, Rune; Jonson, Ida; Moen, Marivi Nabong; Nesse, Gaute; Asker, Gry; Klungland, Arne & Larsen, Elisabeth (2016). Role of ALKBH1 in the Core Transcriptional Network of Embryonic Stem Cells. Cellular Physiology and Biochemistry.  ISSN 1015-8987.  38(1), s 173- 184 . doi: 10.1159/000438619
• Rasmussen, Erik; Vågbø, Cathrine Broberg; Münch, Daniel; Krokan, Hans Einar; Klungland, Arne; Amdam, Gro Vang & Dahl, John Arne (2016). DNA base modifications in honey bee and fruit fly genomes suggest an active demethylation machinery with species- and tissue-specific turnover rates. Biochemistry and Biophysics Reports.  ISSN 2405-5808.  6, s 9- 15 . doi: 10.1016/j.bbrep.2016.02.011 Fulltekst i vitenarkiv.
• Tekpli, Xavier; Urbanucci, Alfonso; Hashim, Adnan; Vågbø, Cathrine Broberg; Lyle, Robert; Kringen, Marianne K.; Staff, Anne Cathrine; Dybedal, Ingunn; Mills, Ian Geoffrey; Klungland, Arne & Staerk, Judith (2016). Changes of 5-hydroxymethylcytosine distribution during myeloid and lymphoid differentiation of CD34+ cells. Epigenetics & Chromatin.  ISSN 1756-8935.  9 . doi: 10.1186/s13072-016-0070-8 Fulltekst i vitenarkiv.
• Vindedal, Gry Fluge; Thoren, Anna; Jensen, Vidar; Klungland, Arne; Zhang, Yong; Holtzman, Michael; Ottersen, Ole Petter & Nagelhus, Erlend Arnulf (2016). Removal of aquaporin-4 from glial and ependymal membranes causes brain water accumulation. Molecular and Cellular Neuroscience.  ISSN 1044-7431.  77, s 47- 52 . doi: 10.1016/j.mcn.2016.10.004
• Weber, Alain R.; Krawczyk, Claudia; Robertson, Adam Brian; Kusnierczyk, Anna; Vågbø, Cathrine Broberg; Schürmann, David; Klungland, Arne & Schär, Primo (2016). Biochemical reconstitution of TET1-TDG-BER-dependent active DNA demethylation reveals a highly coordinated mechanism. Nature Communications.  ISSN 2041-1723.  7 . doi: 10.1038/ncomms10806 Fulltekst i vitenarkiv.
• Bjørge, Monica Dahl; Hildrestrand, Gunn Annette; Scheffler, Katja; Suganthan, Rajikala; Rolseth, Veslemøy; Kusnierczyk, Anna; Rowe, Alexander D.; Vågbø, Cathrine Broberg; Vetlesen, Susanne; Eide, Lars; Slupphaug, Geir; Yusaku, Nakabeppu; Timothy, Bredy; Klungland, Arne & Bjørås, Magnar (2015). Synergistic Actions of Ogg1 and Mutyh DNA Glycosylases Modulate Anxiety-like Behavior in Mice. Cell reports.  ISSN 2211-1247.  13(12), s 2671- 2678 . doi: 10.1016/j.celrep.2015.12.001 Fulltekst i vitenarkiv. Vis sammendrag

  Ogg1 and Mutyh DNA glycosylases cooperate to prevent mutations caused by 8-oxoG, a major premutagenic DNA lesion associated with cognitive decline. We have examined behavior and cognitive function in mice deficient of these glycosylases. Ogg1−/−Mutyh−/− mice were more active and less anxious, with impaired learning ability. In contrast, Mutyh−/− mice showed moderately improved memory. We observed no apparent change in genomic 8-oxoG levels, suggesting that Ogg1 and Mutyh play minor roles in global repair in adult brain. Notably, transcriptome analysis of hippocampus revealed that differentially expressed genes in the mutants belong to pathways known to be involved in anxiety and cognition. Esr1 targets were upregulated, suggesting a role of Ogg1 and Mutyh in repression of Esr1 signaling. Thus, beyond their involvement in DNA repair, Ogg1 and Mutyh regulate hippocampal gene expression related to cognition and behavior, suggesting a role for the glycosylases in regulating adaptive behavior.

• Chymkowitch, Pierre; Nguéa P, Aurélie; Aanes, Håvard; Koehler, Christian; Thiede, Bernd; Lorenz, Susanne; Meza, Leonardo Zepeda; Klungland, Arne & Enserink, Jorrit (2015). Sumoylation of Rap1 mediates the recruitment of TFIID to promote transcription of ribosomal protein genes. Genome Research.  ISSN 1088-9051.  25(6), s 897- 906 . doi: 10.1101/gr.185793.114
• Fusser, Markus; Kernstock, Stefan; Aileni, Vinay Kumar; Jacobsen, Wolfgang Mathias; Falnes, Pål & Klungland, Arne (2015). Lysine methylation of the valosin-containing protein (VCP) is dispensable for development and survival of mice. PLOS ONE.  ISSN 1932-6203.  10(11) . doi: 10.1371/journal.pone.0141472 Fulltekst i vitenarkiv.
• Ke, Shengdong; Alemu, Endalkachew Ashenafi; Mertens, Claudia; Gantman, Emily Conn; Fak, John J.; Mele, Aldo; Haripal, Bhagwattie; Zucker-Scharff, Ilana; Moore, Michael J.; Park, Christopher Y.; Vågbø, Cathrine Broberg; Kusnierczyk, Anna; Klungland, Arne; Darnell, James E. & Darnell, Robert B. (2015). A majority of m6A residues are in the last exons, allowing the potential for 3' UTR regulation. Genes & Development.  ISSN 0890-9369.  29(19), s 2037- 2053 . doi: 10.1101/gad.269415.115
• Lauritzen, Knut Husø; Kleppa, Liv; Aronsen, Jan Magnus; Eide, Lars; Carlsen, Harald; Haugen, Øyvind Pernell; Sjaastad, Ivar; Klungland, Arne; Rasmussen, Lene Juel; Attramadal, Håvard; Storm-Mathisen, Jon & Bergersen, Linda Hildegard (2015). Impaired dynamics and function of mitochondria caused by mtDNA toxicity leads to heart failure. American Journal of Physiology. Heart and Circulatory Physiology.  ISSN 0363-6135.  309(3), s H434- H449 . doi: 10.1152/ajpheart.00253.2014
• Ougland, Rune; Rognes, Torbjørn; Klungland, Arne & Larsen, Elisabeth (2015). Non-homologous functions of the AlkB homologs. Journal of Molecular Cell Biology.  ISSN 1674-2788.  7(6), s 494- 504 . doi: 10.1093/jmcb/mjv029
• Robertson, Adam Brian; Dahl, John Arne & Klungland, Arne (2014). DNA metabolism: bases of DNA repair and regulation.. Nature Chemical Biology.  ISSN 1552-4450.  10(7), s 487- 488 . doi: 10.1038/nchembio.1553
• Robertson, Adam Brian; Robertson, Julia; Fusser, Markus & Klungland, Arne (2014). Endonuclease G preferentially cleaves 5-hydroxymethylcytosine-modified DNA creating a substrate for recombination. Nucleic Acids Research (NAR).  ISSN 0305-1048.  42(21), s 13280- 13293 . doi: 10.1093/nar/gku1032
• Dahl, John Arne & Klungland, Arne (2014). Micro Chromatin Immunoprecipitation (μChIP) from Early Mammalian Embryos. Methods in molecular biology.  ISSN 1064-3745.  1222, s 227- 245 . doi: 10.1007/978-1-4939-1594-1_17
• Klungland, Arne & Dahl, John Arne (2014). Dynamic RNA modifications in disease. Current Opinion in Genetics and Development.  ISSN 0959-437X.  26, s 47- 52 . doi: 10.1016/j.gde.2014.05.006
• Nilsen, Anja; Fusser, Markus; Greggains, Gareth; Fedorcsak, Peter & Klungland, Arne (2014). ALKBH4 depletion in mice leads to spermatogenic defects. PLOS ONE.  ISSN 1932-6203.  9(8) . doi: 10.1371/journal.pone.0105113
• Zdzalik, Daria; Vågbø, Cathrine Broberg; Kirpekar, F; Davydova, Erna; Puścian, Alicja; Maciejewska, Agnieszka M.; Krokan, Hans Einar; Klungland, Arne; Tudek, Barbara; Van den Born, Erwin & Falnes, Pål (2014). Protozoan ALKBH8 Oxygenases Display both DNA Repair and tRNA Modification Activities. PLOS ONE.  ISSN 1932-6203.  9(6) . doi: 10.1371/journal.pone.0098729 Fulltekst i vitenarkiv.
• Solberg, Anja; Robertson, Adam Brian; Aronsen, Jan Magnus; Rognmo, Øivind; Sjaastad, Ivar; Wisløff, Ulrik & Klungland, Arne (2013). Deletion of mouse Alkbh7 leads to obesity. Journal of Molecular Cell Biology.  ISSN 1674-2788.  5(3), s 194- 203 . doi: 10.1093/jmcb/mjt012
• Furu, Kari & Klungland, Arne (2013). Tzfp Represses the Androgen Receptor in Mouse Testis. PLOS ONE.  ISSN 1932-6203.  8(4) . doi: 10.1371/journal.pone.0062314
• Jonson, Ida; Ougland, Rune; Klungland, Arne & Larsen, Elisabeth (2013). Oxidative stress causes DNA triplet expansion in Huntington's disease mouse embryonic stem cells. Stem Cell Research.  ISSN 1873-5061.  11(3), s 1264- 1271 . doi: 10.1016/j.scr.2013.08.010
• Li, Ming-Ming; Nilsen, Anja; Shi, Yue; Fusser, Markus; Ding, Yue-He; Fu, Ye; Liu, Bo; Niu, Yamei; Wu, Yong-Sheng; Huang, Chun-Min; Olofsson, Maria; Jin, Kang-Xuan; Lv, Ying; Xu, Xing-Zhi; He, Chuan; Dong, Meng-Qiu; Danielsen, Jannie M. Rendtlew; Klungland, Arne & Yang, Yun-Gui (2013). ALKBH4-dependent demethylation of actin regulates actomyosin dynamics. Nature Communications.  ISSN 2041-1723.  4 . doi: 10.1038/ncomms2863
• Zheng, Guanqun; Dahl, John Arne; Niu, Yamei; Fedorcsak, Peter; Huang, Chun-Min; Li, Charles J.; Vågbø, Cathrine Broberg; Shi, Yue; Wang, Wen-Ling; Song, Shu-Hui; Lu, Zhike; Bosmans, Ralph P. G.; Dai, Qing; Hao, Ya-Juan; Yang, Xin; Zhao, Wen-Ming; Tong, Wei-Min; Wang, Xiu-Jie; Bogdan, Florian; Furu, Kari; Fu, Ye; Jia, Guifang; Zhao, Xu; Liu, Jun; Krokan, Hans Einar; Klungland, Arne; Yang, Yun-Gui & He, Chuan (2013). ALKBH5 Is a Mammalian RNA Demethylase that Impacts RNA Metabolism and Mouse Fertility. Molecular Cell.  ISSN 1097-2765.  49(1), s 18- 29 . doi: 10.1016/j.molcel.2012.10.015
• Robertson, Adam Brian; Dahl, John Arne; Ougland, Rune & Klungland, Arne (2012). Pull-down of 5-hydroxymethylcytosine DNA using JBP1-coated magnetic beads. Nature Protocols.  ISSN 1754-2189.  7(2), s 340- 350 . doi: 10.1038/nprot.2011.443
• Calvo, Jennifer A; Meira, Lisiane B.; Lee, C.Y.; Moroski-Erkul, Catherine A; Abolhassani, Nona; Taghizadeh, Koli; Eichinger, Lindsey W; Muthupalani, Sureshkumar; Nordstrand, Line Mari; Klungland, Arne & Samson, Leona D. (2012). DNA repair is indispensable for survival after acute inflammation. Journal of Clinical Investigation.  ISSN 0021-9738.  122(7), s 2680- 2689 . doi: 10.1172/JCI63338
• Kleppa, Liv; Mari, Pierre-Olivier; Larsen, Elisabeth; Lien, Guro Flor; Godon, Camille; Theil, Arjan F; Nesse, Gaute; Wiksén, Hege; Vermeulen, Wim; Giglia-Mari, Giuseppina & Klungland, Arne (2012). Kinetics of endogenous mouse FEN1 in base excision repair. Nucleic Acids Research (NAR).  ISSN 0305-1048.  40(18), s 9044- 9059 . doi: 10.1093/nar/gks673
• Meza, Trine Johansen; Moen, Marivi; Vågbø, Cathrine Broberg; Krokan, Hans Einar; Klungland, Arne; Grini, Paul Eivind & Falnes, Pål (2012). The DNA dioxygenase ALKBH2 protects Arabidopsis thaliana against methylation damage. Nucleic Acids Research (NAR).  ISSN 0305-1048.  40(14), s 6620- 6631 . doi: 10.1093/nar/gks327 Fulltekst i vitenarkiv.
• Møllersen, Linda; Rowe, Alexander D.; Illuzzi, Jennifer L.; Hildrestrand, Gunn Annette; Gerhold, Katharina J.; Tveterås, Linda Augustin; Bjølgerud, Anja; Wilson, David M.; Bjørås, Magnar & Klungland, Arne (2012). Neil1 is a genetic modifier of somatic and germline CAG trinucleotide repeat instability in R6/1 mice. Human Molecular Genetics.  ISSN 0964-6906.  21(22), s 4939- 4947 . doi: 10.1093/hmg/dds337
• Nordstrand, Line Mari; Furu, Kari; Paulsen, Jonas; Rognes, Torbjørn & Klungland, Arne (2012). Alkbh1 and Tzfp repress a non-repeat piRNA cluster in pachytene spermatocytes. Nucleic Acids Research (NAR).  ISSN 0305-1048.  40(21), s 10950- 10963 . doi: 10.1093/nar/gks839 Fulltekst i vitenarkiv.
• Ougland, Rune; Lando, David; Jonson, Ida; Dahl, John Arne; Moen, Marivi; Nordstrand, Line Mari; Rognes, Torbjørn; Lee, Jeannie T; Klungland, Arne; Kouzarides, Tony & Larsen, Elisabeth (2012). ALKBH1 Is a Histone H2A Dioxygenase Involved in Neural Differentiation. Stem Cells.  ISSN 1066-5099.  30(12), s 2672- 2682 . doi: 10.1002/stem.1228 Fulltekst i vitenarkiv.
• Lauritzen, Knut Husø; Chang, Cheng; Wiksén, Hege; Bergersen, Linda Hildegard & Klungland, Arne (2011). Mitochondrial DNA toxicity compromises mitochondrial dynamics and induces hippocampal antioxidant defenses. DNA Repair.  ISSN 1568-7864.  10(6), s 639- 653 . doi: 10.1016/j.dnarep.2011.04.011
• Robertson, Adam Brian; Dahl, John Arne; Vågbø, Cathrine Broberg; Tripathi, P; Krokan, Hans Einar & Klungland, Arne (2011). A novel method for the efficient and selective identification of 5-hydroxymethylcytosine in genomic DNA. Nucleic Acids Research (NAR).  ISSN 0305-1048.  39(8) . doi: 10.1093/nar/gkr051 Fulltekst i vitenarkiv.
• Robertson, Julia; Robertson, Adam Brian & Klungland, Arne (2011). The presence of 5-hydroxymethylcytosine at the gene promoter and not in the gene body negatively regulates gene expression. Biochemical and Biophysical Research Communications - BBRC.  ISSN 0006-291X.  411(1), s 40- 43 . doi: 10.1016/j.bbrc.2011.06.077
• Fusser, Markus; Nesse, Gaute; Khobta, Andriy; Xia, Ning; Li, Huige; Klungland, Arne & Epe, Bernd (2011). Spontaneous mutagenesis in Csb(m/m) Ogg1(−/−) mice is attenuated by dietary resveratrol. Carcinogenesis.  ISSN 0143-3334.  32(1), s 80- 85 . doi: 10.1093/carcin/bgq196
• Haj Yasein, Nadia Nabil; Jensen, Vidar; Vindedal, Gry Fluge; Gundersen, Georg Andreas; Klungland, Arne; Ottersen, Ole Petter; Hvalby, Øyvind Christian & Nagelhus, Erlend Arnulf (2011). Evidence that Compromised K+ Spatial Buffering Contributes to the Epileptogenic Effect of Mutations in the Human Kir4.1 Gene (KCNJ10). Glia.  ISSN 0894-1491.  59(11), s 1635- 1642 . doi: 10.1002/glia.21205
• Haj Yasein, Nadia Nabil; Vindedal, Gry Fluge; Eilert-Olsen, Martine; Gundersen, Georg Andreas; Skare, Øivind; Laake, Petter; Klungland, Arne; Thoren, Anna; Burkhardt, John Michael; Ottersen, Ole Petter & Nagelhus, Erlend Arnulf (2011). Glial-conditional deletion of aquaporin-4 (Aqp4) reduces blood-brain water uptake and confers barrier function on perivascular astrocyte endfeet. Proceedings of the National Academy of Sciences of the United States of America.  ISSN 0027-8424.  108(43), s 17815- 17820 . doi: 10.1073/pnas.1110655108
• Lauritzen, Knut Husø; Dalhus, Bjørn; Storm, Johan Frederik; Bjørås, Magnar & Klungland, Arne (2011). Modeling the impact of mitochondrial DNA damage in forebrain neurons and beyond. Mechanisms of Ageing and Development.  ISSN 0047-6374.  132(8-9), s 424- 428 . doi: 10.1016/j.mad.2011.02.006
• Moen, Marivi; Knævelsrud, Ingeborg; Haugland, Gyri Teien; Grøsvik, Kristin; Birkeland, Nils-Kåre; Klungland, Arne & Bjelland, Svein (2011). Uracil-DNA Glycosylase of Thermoplasma acidophilum Directs Long-Patch Base Excision Repair, Which Is Promoted by Deoxynucleoside Triphosphates and ATP/ADP, into Short-Patch Repair. Journal of Bacteriology.  ISSN 0021-9193.  193(17), s 4495- 4508 . doi: 10.1128/JB.00233-11
• Strømme, Petter; Dobrenis, Kostantin; Sillitoe, Roy V.; Gulinello, Maria; Ali, Nafeeza F.; Davidson, Christin; Micsenyi, Matthew C.; Stephney, Gloria; Ellevog, Linda; Klungland, Arne & Walkley, Steven U. (2011). X-linked Angelman-like syndrome caused by Slc9a6 knockout in mice exhibits evidence of endosomal-lysosomal dysfunction. Brain.  ISSN 0006-8950.  134, s 3369- 3383 . doi: 10.1093/brain/awr250
• Thrane, Alexander Stanley; Rappold, Philip M; Fujita, Takumi; Torres, Arnulfo; Bekar, Lane K; Takano, Takahiro; Peng, Weiguo; Wang, Fushun; Thrane Rangroo, Vinita; Enger, Rune; Haj Yasein, Nadia Nabil; Skare, Øivind; Holen, Torgeir; Klungland, Arne; Ottersen, Ole Petter; Nedergaard, Maiken & Nagelhus, Erlend Arnulf (2011). Critical role of aquaporin-4 (AQP4) in astrocytic Ca2+ signaling events elicited by cerebral edema. Proceedings of the National Academy of Sciences of the United States of America.  ISSN 0027-8424.  108(2), s 846- 851 . doi: 10.1073/pnas.1015217108
• Van den Born, Erwin; Vågbø, Cathrine Broberg; Songe-Møller, Lene; Leihne, VIbeke; Lien, Guro Flor; Leszczynska, G; Malkiewicz, A; Krokan, Hans Einar; Kirpekar, F; Klungland, Arne & Falnes, Pål (2011). ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA. Nature Communications.  ISSN 2041-1723.  2 . doi: 10.1038/ncomms1173 Fulltekst i vitenarkiv.
• Dahl, John Arne; Reiner, Andrew Henry; Klungland, Arne; Wakayama, Teruhiko & Collas, Philippe (2010). Histone H3 Lysine 27 Methylation Asymmetry on Developmentally-Regulated Promoters Distinguish the First Two Lineages in Mouse Preimplantation Embryos. PLOS ONE.  ISSN 1932-6203.  5(2) . doi: 10.1371/journal.pone.0009150
• Knævelsrud, Ingeborg; Moen, Marivi; Grøsvik, Kristin; Haugland, Gyri Teien; Birkeland, Nils-Kåre; Klungland, Arne; Leiros, Ingar & Bjelland, Svein (2010). The Hyperthermophilic Euryarchaeon Archaeoglobus fulgidus Repairs Uracil by Single-Nucleotide Replacement. Journal of Bacteriology.  ISSN 0021-9193.  192(21), s 5755- 5766 . doi: 10.1128/JB.00135-10 Vis sammendrag

  Hydrolytic deamination of cytosine to uracil in cellular DNA is a major source of C-to-T transition mutations if uracil is not repaired by the DNA base excision repair (BER) pathway. Since deamination increases rapidly with temperature, hyperthermophiles, in particular, are expected to succumb to such damage. There has been only one report of crenarchaeotic BER showing strong similarities to that in most eukaryotes and bacteria for hyperthermophilic Archaea. Here we report a different type of BER performed by extract prepared from cells of the euryarchaeon Archaeoglobus fulgidus. Although immunodepletion showed that the monofunctional family 4 type of uracil-DNA glycosylase (UDG) is the principal and probably only UDG in this organism, a beta-elimination mechanism rather than a hydrolytic mechanism is employed for incision of the abasic site following uracil removal. The resulting 3' remnant is removed by efficient 3'-phosphodiesterase activity followed by single-nucleotide insertion and ligation. The finding that repair product formation is stimulated similarly by ATP and ADP in vitro raises the question of whether ADP is more important in vivo because of its higher heat stability.

• Lauritzen, Knut Husø; Moldestad, Olve; Eide, Lars; Carlsen, Harald; Nesse, Gaute; Storm, Johan Frederik; Mansuy, Isabelle M.; Bergersen, Linda Hildegard & Klungland, Arne (2010). Mitochondrial DNA Toxicity in Forebrain Neurons Causes Apoptosis, Neurodegeneration, and Impaired Behavior. Molecular and Cellular Biology.  ISSN 0270-7306.  30(6), s 1357- 1367 . doi: 10.1128/MCB.01149-09 Vis sammendrag

  Mitochondrial dysfunction underlying changes in neurodegenerative diseases is often associated with apoptosis and a progressive loss of neurons, and damage to the mitochondrial genome is proposed to be involved in such pathologies. In the present study we designed a mouse model that allows us to specifically induce mitochondrial DNA toxicity in the forebrain neurons of adult mice. This is achieved by CaMKII-regulated inducible expression of a mutated version of the mitochondrial UNG DNA repair enzyme (mutUNG1). This enzyme is capable of removing thymine from the mitochondrial genome. We demonstrate that a continual generation of apyrimidinic sites causes apoptosis and neuronal death. These defects are associated with behavioral alterations characterized by increased locomotor activity, impaired cognitive abilities, and lack of anxietylike responses. In summary, whereas mitochondrial base substitution and deletions previously have been shown to correlate with premature and natural aging, respectively, we show that a high level of apyrimidinic sites lead to mitochondrial DNA cytotoxicity, which causes apoptosis, followed by neurodegeneration.

• Møllersen, Linda; Rowe, Alexander D.; Larsen, Elisabeth; Rognes, Torbjørn & Klungland, Arne (2010). Continuous and Periodic Expansion of CAG Repeats in Huntington's Disease R6/1 Mice. PLoS Genetics.  ISSN 1553-7390.  6(12) . doi: 10.1371/journal.pgen.1001242 Fulltekst i vitenarkiv.
• Nordstrand, Line Mari; Svärd, Jessica; Larsen, Elisabeth; Nilsen, Anja; Ougland, Rune; Furu, Kari; Lien, Guro Flor; Rognes, Torbjørn; Namekawa, Satoshi H; Lee, Jeannie T & Klungland, Arne (2010). Mice Lacking Alkbh1 Display Sex-Ratio Distortion and Unilateral Eye Defects. PLOS ONE.  ISSN 1932-6203.  5(11) . doi: 10.1371/journal.pone.0013827 Fulltekst i vitenarkiv.
• Songe-Møller, Lene; Van den Born, Erwin; Leihne, V; Vågbø, Cathrine Broberg; Kristoffersen, T; Krokan, Hans Einar; Kirpekar, F; Falnes, PO & Klungland, A (2010). Mammalian ALKBH8 Possesses tRNA Methyltransferase Activity Required for the Biogenesis of Multiple Wobble Uridine Modifications Implicated in Translational Decoding. Molecular and Cellular Biology.  ISSN 0270-7306.  30(7), s 1814- 1827 . doi: 10.1128/MCB.01602-09
• Robertson, AB; Klungland, A; Rognes, T & Leiros, Ingar (2009). DNA Repair in Mammalian Cells. Cellular and Molecular Life Sciences (CMLS).  ISSN 1420-682X.  66(6), s 981- 993 . doi: 10.1007/s00018-009-8736-z
• Van den Born, Erwin; Bekkelund, Anders; Moen, Marivi; Omelchenko, M.V.; Klungland, Arne & Falnes, Pål (2009). Bioinformatics and functional analysis define four distinct groups of AlkB DNA-dioxygenases in bacteria. Nucleic Acids Research (NAR).  ISSN 0305-1048.  37(21) . doi: 10.1093/nar/gkp774
• Larsen, Elisabeth; Kleppa, Liv; Meza, Trine Johansen; Meza, Zepeda Leonardo; Rada, C; Castellanos, Cesilie Granum; Lien, Guro Flor; Nesse, Gaute; Neuberger, MS; Lærdahl, Jon Kristen; Doughty, RW & Klungland, Arne (2008). Early-onset lymphoma and extensive embryonic apoptosis in two domain-specific Fen1 mice mutants. Cancer Research.  ISSN 0008-5472.  68, s 4571- 4579 . doi: 10.1158/0008-5472.CAN-08-0168
• Ringvoll, Jeanette; Moen, Marivi N.; Nordstrand, Line M.; Meira, Lisiane B.; Pang, Bo; Bekkelund, Anders; Dedon, Peter C.; Bjelland, Svein; Samson, Leona D.; Falnes, Pål Ø. & Klungland, Arne (2008). AlkB homologue 2-mediated repair of ethenoadenine lesions in mammalian DNA. Cancer Research.  ISSN 0008-5472.  68(11), s 4142- 4149 Vis sammendrag

  Endogenous formation of the mutagenic DNA adduct 1,N-6-ethenoadenine (epsilon A) originates from lipid peroxidation. Elevated levels of epsilon A in cancer-prone tissues suggest a role for this adduct in the development of some cancers. The base excision repair pathway has been considered the principal repair system for epsilon A lesions until recently, when it was shown that the Escherichia coli AlkB dioxygenase could directly reverse the damage. We report here kinetic analysis of the recombinant human AlkB homologue 2 (hABH2), which is able to repair epsilon A lesions in DNA. Furthermore, cation exchange chromatography of nuclear extracts from wild-type and mABH2(-/-) mice indicates that mABH2 is the principal dioxygenase for EA repair in vivo. This is further substantiated by experiments showing that hABH2, but not hABH3, is able to complement the E. coli alkB mutant with respect to its defective repair of etheno adducts. We conclude that ABH2 is active in the direct reversal of epsilon A lesions, and that ABH2, together with the alkyl-N-adenine-DNA glycosylase, which is the most effective enzyme for the repair of epsilon A, comprise the cellular defense against epsilon A lesions.

• Saxowsky, TT; Meadows, KL; Klungland, Arne & Doetsch, PW (2008). 8-Oxoguanine-mediated transcriptional mutagenesis causes Ras activation in mammalian cells. Proceedings of the National Academy of Sciences of the United States of America.  ISSN 0027-8424.  105(48), s 18877- 18882 . doi: 10.1073/pnas.0806464105
• Wærsted, Jeanette Ringvoll; Nabong, Marivi; Nordstrand, Line Mari; Meira, LB; Pang, B; Bekkelund, Anders; Dedon, PC; Bjelland, S; Samson, LD; Falnes, Pål & Klungland, Arne (2008). AlkB homologue 2-mediated repair of ethenoadenine lesions in mammalian DNA. Cancer Research.  ISSN 0008-5472.  68(11), s 4142- 4149 . doi: 10.1158/0008-5472.CAN-08-0796 Vis sammendrag

  Endogenous formation of the mutagenic DNA adduct 1,N6-ethenoadenine ({varepsilon}A) originates from lipid peroxidation. Elevated levels of {varepsilon}A in cancer-prone tissues suggest a role for this adduct in the development of some cancers. The base excision repair pathway has been considered the principal repair system for {varepsilon}A lesions until recently, when it was shown that the Escherichia coli AlkB dioxygenase could directly reverse the damage. We report here kinetic analysis of the recombinant human AlkB homologue 2 (hABH2), which is able to repair {varepsilon}A lesions in DNA. Furthermore, cation exchange chromatography of nuclear extracts from wild-type and mABH2–/– mice indicates that mABH2 is the principal dioxygenase for {varepsilon}A repair in vivo. This is further substantiated by experiments showing that hABH2, but not hABH3, is able to complement the E. coli alkB mutant with respect to its defective repair of etheno adducts. We conclude that ABH2 is active in the direct reversal of {varepsilon}A lesions, and that ABH2, together with the alkyl-N-adenine-DNA glycosylase, which is the most effective enzyme for the repair of {varepsilon}A, comprise the cellular defense against {varepsilon}A lesions. [Cancer Res 2008;68(11):4142–9]

• Falnes, Pål; Klungland, Arne & Alseth, Ingrun (2007). Repair of methyl lesions in DNA and RNA by oxidative demethylation. Neuroscience.  ISSN 0306-4522.  145 . doi: 10.1016/j.neuroscience.2006.11.018
• Kleppa, Liv; Kanavin, Øivind Juris; Klungland, Arne & Strømme, Petter (2007). A novel splice site mutation in the Cockayne syndrome group A gene in two siblings with Cockayne syndrome. Neuroscience.  ISSN 0306-4522.  4, s 1397- 1406 . doi: 10.1016/j.neuroscience.2006.09.025
• Klungland, Arne & Bjelland, Svein (2007). Oxidative damage to purines in DNA: Role of mammalian Ogg1. DNA Repair.  ISSN 1568-7864.  6 Vis sammendrag

  DNA damage caused by reactive oxygen species is ubiquitous to all living organisms. More than 60 different base lesions have been identified, and the majority of these are removed via the base excision repair pathway. This pathway appears to represent a highly conserved and ancient mechanism of defence counteracting spontaneous DNA decay. In this review, we describe in more detail the Ogg1 enzyme and its conserved action of removing the oxidised base, 7,8-dihydro-8-oxoguanine (oxo(8)G). Recent updates include the cancer-prone ogg1/myh double knockout mouse and an elegant study which looks at the ability of hOgg1 to distinguish between the mutagenic lesion, oxo(8)G, and the vast majority of normal bases. (c) 2006 Elsevier B.V. All rights reserved.

• Klungland, Arne; Lærdahl, Jon Kristen & Rognes, Torbjørn (2007). OGG1: From Structural Analysis to the Knockout Mouse, In Mark D. Evans & Marcus S. Cooke (ed.),  Oxidative Damage to Nucleic Acids.  Springer Science+Business Media B.V..  ISBN 9780387729732.  Chapter 5.  s 67 - 80
• Kovtun, IV; Liu, Y; Bjørås, Magnar; Klungland, Arne; Wilson, SH & McMurray, CT (2007). OGG1 initiates age-dependent CAG trinucleotide expansion in somatic cells. Nature.  ISSN 0028-0836.  447 . doi: 10.1038/nature05778
• Larsen, E; Meza, TJ; Kleppa, L & Klungland, Arne (2007). Organ and cell specificity of base excision repair mutants in mice. Mutation research.  ISSN 0027-5107.  614, s 56- 68 . doi: 10.1016/j.mrfmmm.2006.01.023
• Leiros, Ingar; Nabong, Marivi P.; Grøsvik, Kristin; Ringvoll, Jeanette; Haugland, GT; Uldal, Lene; Reite, Karen; Olsbu, Inger K.; Knævelsrud, I.; Moe, Elin; Andersen, Ole A.; Birkeland, Nils-Kåre; Ruoff, Peter; Klungland, Arne & Bjelland, Svein (2007). Structural basis for enzymatic excision of N-1-methyladenine and N-3-methylcytosine from DNA. EMBO Journal.  ISSN 0261-4189.  26 Vis sammendrag

  N-1-methyladenine (m(1)A) and N-3-methylcytosine (m(3)C) are major toxic and mutagenic lesions induced by alkylation in single-stranded DNA. In bacteria and mammals, m 1 A and m 3 C were recently shown to be repaired by AlkB-mediated oxidative demethylation, a direct DNA damage reversal mechanism. No AlkB gene homologues have been identified in Archaea. We report that m(1)A and m(3)C are repaired by the AfAlkA base excision repair glycosylase of Archaeoglobus fulgidus, suggesting a different repair mechanism for these lesions in the third domain of life. In addition, AfAlkA was found to effect a robust excision of 1, N-6-ethenoadenine. We present a high-resolution crystal structure of AfAlkA, which, together with the characterization of several site-directed mutants, forms a molecular rationalization for the newly discovered base excision activity.

• Leiros, Ingar; Nabong, Marivi; Nabong, MP; Grosvik, K; Grøsvik, Kristin; Ringvoll, J; Ringvoll, Jeanette; Haugland, GT; Uldal, Lene; Uldal, L; Reite, K; Olsbu, IK; Knævelsrud, Ingeborg; Knaevelsrud, I; Moe, Elin; Andersen, Ole Andreas; Birkeland, NK; Ruoff, P; Klungland, A & Bjelland, S (2007). Structural basis for enzymatic excision of N-1-methyladenine and N-3-methylcytosine from DNA. EMBO Journal.  ISSN 0261-4189.  26 . doi: 10.1038/sj.emboj.7601662
• Nordstrand, Line Mari; Ringvoll, Jeanette; Larsen, Elisabeth & Klungland, Arne (2007). Genome instability and DNA damage accumulation in gene-targeted mice. Neuroscience.  ISSN 0306-4522.  145 . doi: 10.1016/j.neuroscience.2006.10.059
• Ringvoll, Jeanette; Uldal, Lene; Røed, Marie Aslaksen; Stenersen, Karen Oppland; Baynton, K; Klungland, Arne & Eide, Lars (2007). Mutations in the RAD27 and SGS1 genes differentially affect the chronological and replicative lifespan of yeast cells growing on glucose and glycerol. FEMS Yeast Research.  ISSN 1567-1356.  7, s 848- 859 . doi: 10.1111/j.1567-1364.2007.00248.x
• Ringvoll, Jeanette; Ringvoll, Jeanette; Nordstrand, Line M.; Vågbø, Cathrine Broberg; Vagbo, CB; Talstad, Vivi; Reite, Karen; Aas, Per Arne; Lauritzen, Knut H.; Liabakk, Nina-Beate; Bjørk, Alexandra; Doughty, Richard William; Falnes, Pål Ø.; Krokan, Hans Einar & Klungland, Arne (2006). Repair deficient mice reveal mABH2 as the primary oxidative demethylase for repairing 1meA and 3meC lesions in DNA. EMBO Journal.  ISSN 0261-4189.  25, s 2189- 2198
• Kleppa, Liv; Kanavin, Ø.J; Klungland, Arne & Strømme, Petter (2006). A novel splice site mutation in the Cockayne syndrome group A gene in two siblings with Cockayne syndrome. Neuroscience.  ISSN 0306-4522. . doi: 10.1016/j.neuroscience
• Larsen, Elisabeth; Stenersen, Karen Oppland; Nesse, Gaute; Neurauter, Christine Gran; Seeberg, Erling Christen & Klungland, Arne (2006). Repair and mutagenesis at oxidized DNA lesions in the developing brain of wild-type and Ogg1(-/-) mice. Oncogene.  ISSN 0950-9232.  25, s 2425- 2432
• Touati, E; Michel, V; Thiberge, JM; Ave, P; Huerre, M; Bourgade, F; Klungland, Arne & Labigne, A (2006). Deficiency in OGG1 protects against inflammation and mutagenic effects associated with H-Pylori infection in mouse. Helicobacter.  ISSN 1083-4389.  11, s 494- 505
• Holen, Torgeir; Moe, Svein Erik E; Sørbø, Jan Gunnar; Meza, Trine Johansen; Ottersen, Ole Petter & Klungland, Arne (2005). Tolerated wobble mutations in siRNAs decrease specificity, but can enhance activity in vivo. Nucleic Acids Research (NAR).  ISSN 0305-1048.  33(15), s 4704- 4710 . doi: 10.1093/nar/gki785
• Rozalski, R; Siomek, A; Gackowski, D; Foksinski, M; Gran, C; Klungland, Arne & Olinski, R (2005). Substantial decrease of urinary 8-oxo-7,8-dihydroguanine, a product of the base excision repair pathway, in DNA glycosylase defective mice. International Journal of Biochemistry and Cell Biology.  ISSN 1357-2725.  37
• Rozalski, R; Siomek, A; Gackowski, D; Gran, Christine; Foksinski, M; Gran, C; Klungland, Arne & Olinski, R (2004). Diet is not responsible for the presence of several oxidatively damaged DNA lesions in mouse urine. Free radical research.  ISSN 1071-5762.  38(11), s 1201- 1205
• Foksinski, M; Rozalski, R; Guz, J; Ruszkowska, B; Sztukowska, P; Piwowarski, M; Klungland, Arne & Olinski, R (2004). Urinary excretion of DNA repair products correlates with metabolic rates as well as with maximum life spans of different mammalian species. Free Radical Biology & Medicine.  ISSN 0891-5849.  37(9), s 1449- 1454
• Larsen, E; Kwon, K; Coin, F; Egly, JM & Klungland, Arne (2004). Transcription activities at 8-oxoG lesions in DNA. DNA Repair.  ISSN 1568-7864.  3(11), s 1457- 1468
• Birkeland, Nils-Kåre; Ånensen, Hilde; Knævelsrud, Ingeborg; Kristoffersen, Wenche; Bjørås, M.; Robb, F.T.; Klungland, Arne & Bjelland, Svein (2002). Methylpurine DNA glycosylase of the hyperthermophilic archaeon Archaeoglobus fulgidus. Biochemistry.  ISSN 0006-2960.  41, s 12697- 12705
• Birkeland, Nils-Kåre; Ånensen, Hilde; Knævelsrud, Ingeborg; Kristoffersen, Wenche; Bjørås, M.; Robb, F.T.; Klungland, Arne & Bjelland, Svein (2002). Methylpurine DNA glycosylase of the hyperthermophilic archaeon Archaeoglobus fulgidus. Biochemistry.  ISSN 0006-2960.  41, s 12697- 12705
• Bjelland, Svein; Klungland, Arne; Paulsen, R.; Rolseth, V.; Yamada, Y.; Ueno, Y.; Wiik, P.; Matsuda, A. & Seeberg, E. (2001). 5-Formyluracil and its nucleoside derivatives confer toxicity and mutagenicity to mammalian cells by interfering with normal RNA and DNA metabolism. Toxicology Letters.  ISSN 0378-4274.  119, s 71- 78
• Klungland, Helge & Klungland, Arne (2000). År 2000-2100: Genetikkens århundre?. Kirke og kultur.  ISSN 0023-186X.  105(05.jun), s 461- 476

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• Tesfahun, Almaz Nigatu; Alexeeva, Marina; Tomkuviene, Migle; Laerdahl, Jon Kristen; Klungland, Arne; Klimašauskas, Saulius & Bjelland, Svein (2018). Formamidopyrimidine-DNA glycosylase is involved in C:C mismatch repair.
• Alexeeva, Marina; Moen, Marivi Nabong; Xu, Xiang Ming; Rasmussen, Anette; Kirpekar, Finn; Klungland, Arne & Bjelland, Svein (2017). hSMUG1 is a bi-functional DNA glycosylase.
• Tesfahun, Almaz Nigatu; Alexeeva, Marina; Guragain, Prashanna; Tomkuviene, Migle; Arshad, Aysha; Klungland, Arne; Klimašauskas, Saulius & Bjelland, Svein (2017). Repair of double methylated bases in DNA.
• Tesfahun, Almaz Nigatu; Guragain, Prashanna; Alexeeva, Marina; Arshad, Aysha; Tomkuviene, Migle; Laerdahl, Jon K.; Klungland, Arne; Klimašauskas, Saulius & Bjelland, Svein (2017). Repair of dimethylated bases in DNA.
• Tesfahun, Almaz Nigatu; Klungland, Arne; Klimasauskas, Saulius & Bjelland, Svein (2017). Repair of double methylated bases in DNA.
• Alexeeva, Marina; Moen, Marivi Nabong; Xu, Xiang Ming; Rasmussen, Anette; Kirpekar, Finn; Klungland, Arne & Bjelland, Svein (2016). hSMUG1-catalysed uracil-DNA strand incision.
• Alexeeva, Marina; Moen, Marivi Nabong; Xu, Xiang Ming; Rasmussen, Anette; Kirpekar, Finn; Klungland, Arne & Bjelland, Svein (2016). hSMUG1-catalysed uracil-DNA strand incision.
• Tesfahun, Almaz Nigatu; Klungland, Arne; Klimašauskas, Saulius & Bjelland, Svein (2016). Repair of double methylated bases in DNA.
• Tesfahun, Almaz Nigatu; Klungland, Arne; Klimasauskas, Saulius & Bjelland, Svein (2016). Repair of double methylated bases in DNA.
• Catterall, Tara Cherie; Johnson, Inna; Khan, Shaista; Jermstad, Ingunn; Nesse, Gaute; Klungland, Arne & Dalen, Knut Tomas (2015). The Norwegian Transgenic Centre.
• Khan, Shaista; Jermstad, Ingunn; Catterall, Tara Cherie; Nesse, Gaute; Klungland, Arne & Dalen, Knut Tomas (2014). The Norwegian Transgenic Centre.
• Rasmussen, Erik; Vågbø, Cathrine Broberg; Dahl, John Arne; Münch, Daniel; Krokan, Hans Einar; Klungland, Arne & Amdam, Gro Vang (2014). Epigenetically modified cytosines in the honey bee genome.
• Rasmussen, Erik; Vågbø, Cathrine Broberg; Dahl, John Arne; Münch, Daniel; Krokan, Hans Einar; Klungland, Arne & Amdam, Gro Vang (2013). Presence of modified cytosines in the honey bee genome.
• Zheng, Guanqun; Dahl, John Arne; Niu, Yamei; Fu, Ye & Klungland, Arne (2013). Sprouts of RNA epigenetics The discovery of mammalian RNA demethylases. RNA Biology.  ISSN 1547-6286.  10(6), s 915- 918 . doi: 10.4161/rna.24711
• Warken, D.; Khobta, Andriy; Klungland, Arne & Epe, B. (2010). Flap endonuclease 1 (FEN-1) takes part in repair of DNA single-strand breaks. Naunyn-Schmiedeberg's Archives of Pharmacology.  ISSN 0028-1298.  381, s 73- 73
• Falnes, Pål; Van den Born, Erwin; Leihne, VIbeke; Meza, Trine Johansen; Songe-Møller, Lene & Klungland, Arne (2009). AlkB-like dioxygenases-roles in nucleic acid repair and modification.
• Klungland, Arne & Andersson, Kristin Brevik (2007). Nobelpris for genmodifisering. Tidsskrift for Den norske legeforening.  ISSN 0029-2001.  23, s 127- 127
• Leiros, Ingar; Nabong, Marivi P.; Haugland, GT; Ringvoll, Jeanette; Grøsvik, K; Grøsvik, K; Moe, Elin; Knævelsrud, I.; Knævelsrud, I.; Reite, Karen; Andersen, Ole A.; Birkeland, Nils-Kåre; Klungland, Arne; Bjelland, Svein & Bjelland, Svein (2006). Excision of 1-methyladenine and 3-methylcytosine from DNA by AfAlkA of Archaeoglobus fulgidus.
• Leiros, Ingar; Nabong, Marivi P.; Haugland, Gyri Teien; Ringvoll, Jeanette; Grøsvik, Kristin; Moe, Elin; Knævelsrud, Ingeborg; Reite, Karen; Andersen, Ole Andreas; Birkeland, Nils-Kåre; Klungland, Arne & Bjelland, Svein (2006). Excision of 1-methyladenine and 3-methylcytosine from DNA by AfAlkA of Archaeoglobus fulgidus..
• Trapp, C; Klungland, Arne & Epe, B (2006). The peroxisome proliferator WY-14,643 does not induce oxidative DNA damage in repair-deficient Csb(-/-)Ogg1(-/-) knockout mice. Naunyn-Schmiedeberg's Archives of Pharmacology.  ISSN 0028-1298.  372, s 112- 112
• Bjelland, Svein; Knævelsrud, Ingeborg; Haugland, Gyri Teien; Klungland, Arne & Birkeland, Nils-Kåre (2005). Excision of dUMP from DNA by the hyperthermophilic archaeon Archaeoglobus fulgidus..
• Meza, Trine Johansen; Elisabeth, Larsen; Nesse, Gaute & Klungland, Arne (2005). Knock-down studies of FEN1 by gene targeting and RNAi.
• Meza, Trine Johansen; Elisabeth, Larsen; Halsne, Ruth & Klungland, Arne (2004). Knock-down studies of FEN1 by gene targeting and RNAi.
• Birkeland, Nils-Kåre; Ånensen, Hilde; Kristoffersen, Wenche; Knævelsrud, Ingeborg; Bjørås, M.; Klungland, Arne; Robb, F.T. & Bjelland, Svein (2002). Cold-active methylpurine DNA glycosylase of the hyperthermophilic archaeon Archaeoglobus fulgidus.
• Birkeland, Nils-Kåre; Ånensen, Hilde; Kristoffersen, Wenche; Knævelsrud, Ingeborg; Bjørås, M.; Klungland, Arne; Robb, F.T. & Bjelland, Svein (2002). Cold-active methylpurine DNA glycosylase of the hyperthermophilic archaeon Archaeoglobus fulgidus.
• Klungland, Arne; Klungland, Helge & Kjær, Jan Erik (1998). Derfor er dyrene forskjellige. Buskap.  ISSN 0007-7194.  50(4), s 10- 11
• Klungland, Helge; Klungland, Arne & Kjær, Jan Erik (1998). Gener i avlsarbeidet. Buskap.  ISSN 0007-7194.  50(5), s 20- 22
• Klungland, Helge; Klungland, Arne & Kjær, Jan Erik (1998). Gener og kromosomer. Buskap.  ISSN 0007-7194.  50(3), s 36- 37

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