ebrinch

• Frerker, Nadine; Karlsen, Tommy Aleksander; Lilledahl, Magnus Borstad; Brorson, Sverre Henning; Tibballs, John Earl & Brinchmann, Jan E. (2021). Scaffold-Free Engineering of Human Cartilage Implants. Cartilage. ISSN 1947-6035. 13(1), s. 1237S–1249S. doi: 10.1177/19476035211007923. Fulltekst i vitenarkiv Vis sammendrag

  Abstract Objective. Despite new strategies in tissue engineering, cartilage repair remains a major challenge. Our aim is to treat patients with focal lesions of articular cartilage with autologous hyaline cartilage implants using a scaffold-free approach. In this article, we describe experiments to optimize production of scaffold-free cartilage discs. Design. Articular chondrocytes were expanded in vitro, seeded in transwell inserts and redifferentiated using established chondrogenic components. Experimental variables included testing 2 different expansion media, adding bone morphogenetic protein 2 (BMP2), insulin-like growth factor 1 (IGF1), growth/differentiation factor 5 (GDF5), or fibroblast growth factor 18 (FGF18) to the differentiation medium and allowing the disc to float freely in large wells. Cartilage discs were analyzed by weight and thickness, real-time RT-qPCR (reverse transcriptase qualitative polymerase chain reaction), fluorescence immunostaining, transmission electron microscopy, second harmonic generation imaging, and measurement of Young’s modulus. Results. Addition of BMP2 to the chondrogenic differentiation medium (CDM) was essential for stable disc formation, while IGF1, GDF5, and FGF18 were redundant. Allowing discs to float freely in CDM on a moving platform increased disc thickness compared with discs kept continuously in transwell inserts. Discs cultured for 6 weeks reached a thickness of almost 2 mm and Young’s modulus of >200 kPa. There was abundant type II collagen. Collagen fibrils were 25 nm thick, with a tendency to be organized perpendicular to the disc surface. Conclusion. Scaffold-free engineering using BMP2 and providing free movement in CDM produced firm, elastic cartilage discs with abundant type II collagen. This approach may potentially be used in clinical trials

• Idaszek, Joanna; Costantini, Marco; Karlsen, Tommy Aleksander; Jaroszewicz, Jakub; Colosi, Cristina & Testa, Stefano [Vis alle 16 forfattere av denne artikkelen] (2019). 3D bioprinting of hydrogel constructs with cell and material gradients for the regeneration of full-thickness chondral defect using a microfluidic printing head. Biofabrication. ISSN 1758-5082. 11(4), s. 1–15. doi: 10.1088/1758-5090/ab2622.
• Karlsen, Tommy Aleksander; Aae, Tommy Frøseth & Brinchmann, Jan E. (2019). Robust profiling of microRNAs and isomiRs in human plasma exosomes across 46 individuals. Scientific Reports. ISSN 2045-2322. 9:19999, s. 1–9. doi: 10.1038/s41598-019-56593-7. Fulltekst i vitenarkiv
• Suliman, Salwa; Ali, Hassan Mohamed Abdel-Raouf; Karlsen, Tommy Aleksander; Amiaud, Jérôme; Mohamed Ahmed, Samih & Layrolle, Pierre [Vis alle 9 forfattere av denne artikkelen] (2019). Impact of humanised isolation and culture conditions on stemness and osteogenic potential of bone marrow derived mesenchymal stromal cells. Scientific Reports. ISSN 2045-2322. 9, s. 1–18. doi: 10.1038/s41598-019-52442-9. Fulltekst i vitenarkiv
• Al-Modawi, Rua Nader; Brinchmann, Jan E. & Karlsen, Tommy Aleksander (2019). Multi-pathway protective effects of microRNAs on human chondrocytes in an in vitro model of osteoarthritis. Molecular Therapy - Nucleic Acids. ISSN 2162-2531. 17, s. 776–790. doi: 10.1016/j.omtn.2019.07.011. Fulltekst i vitenarkiv
• Kosik-Koziol, Alicja; Costantini, Marco; Mroz, Anna; Idaszek, Joanna; Heljak, Marcin & Jaroszewicz, Jakub [Vis alle 12 forfattere av denne artikkelen] (2019). 3D bioprinted hydrogel model incorporating β-tricalcium phosphate for calcified cartilage tissue engineering. Biofabrication. ISSN 1758-5082. 11(3). doi: 10.1088/1758-5090/ab15cb.
• Karlsen, Tommy Aleksander; Meenakshi Sundaram, Arvind Yegambaram & Brinchmann, Jan E. (2019). Single-Cell RNA Sequencing of In Vitro Expanded Chondrocytes: MSC-Like Cells With No Evidence of Distinct Subsets. Cartilage. ISSN 1947-6035. doi: 10.1177/1947603519847746.
• Karlsen, Tommy Aleksander & Brinchmann, Jan E. (2019). Expression of inflammatory cytokines in mesenchymal stromal cells is sensitive to culture conditions and simple cell manipulations . Experimental Cell Research. ISSN 0014-4827. 374(1), s. 122–127. doi: 10.1016/j.yexcr.2018.11.014. Fulltekst i vitenarkiv
• Rambøl, Mia Marie-Elisabeth; Hisdal, Jonny; Sundhagen, Jon Otto; Brinchmann, Jan E. & Rosales, Antonio (2018). Recellularization of decellularized venous grafts using peripheral blood: a critical evaluation. EBioMedicine. ISSN 2352-3964. 32, s. 215–222. doi: 10.1016/j.ebiom.2018.05.012. Fulltekst i vitenarkiv
• Brinchmann, Bendik Christian; Skuland, Tonje Schwach; Rambøl, Mia Marie-Elisabeth; Szöke, Krisztina; Brinchmann, Jan E. & Gutleb, Arno C. [Vis alle 16 forfattere av denne artikkelen] (2018). Lipophilic components of diesel exhaust particles induce pro-inflammatory responses in human endothelial cells through AhR dependent pathway(s). Particle and Fibre Toxicology. ISSN 1743-8977. 15. doi: 10.1186/s12989-018-0257-1. Fulltekst i vitenarkiv
• Kosik-Kozioł, Alicja; Costantini, Marco; Bolek, Tomasz; Szöke, Krisztina; Barbetta, Andrea & Brinchmann, Jan E. [Vis alle 7 forfattere av denne artikkelen] (2017). PLA short sub-micron fiber reinforcement of 3D bioprinted alginate constructs for cartilage regeneration. Biofabrication. ISSN 1758-5082. 9:044105(4), s. 1–14. doi: 10.1088/1758-5090/aa90d7.
• Wollert, Kai C.; Meyer, Gerd P.; Müller-Ehmsen, Jochen; Tschöpe, Carsten; Bonarjee, Vernon V S & Larsen, Alf Inge [Vis alle 30 forfattere av denne artikkelen] (2017). Intracoronary autologous bone marrow cell transfer after myocardial infarction: The BOOST-2 randomised placebo-controlled clinical trial. European Heart Journal. ISSN 0195-668X. 38(39), s. 2936–2943. doi: 10.1093/eurheartj/ehx188.
• Szöke, Krisztina; Daňková, Jana; Buzgo, Matej; Amler, Evžen; Brinchmann, Jan E. & Østrup, Esben (2017). The effect of medium composition on deposition of collagen type 1 and expression of osteogenic genes in mesenchymal stem cells derived from human adipose tissue and bone marrow. Process Biochemistry. ISSN 1359-5113. 59, s. 321–328. doi: 10.1016/j.procbio.2016.10.011.
• Behnan, Jinan; Stangeland, Biljana; Hosainey, Sayied Abdol Mohieb; Joel, Mrinal; Olsen, Thale Kristin & Micci, Francesca [Vis alle 9 forfattere av denne artikkelen] (2017). Differential propagation of stroma and cancer stem cells dictates tumorigenesis and multipotency. Oncogene. ISSN 0950-9232. 36(4), s. 570–584. doi: 10.1038/onc.2016.230. Vis sammendrag

  Glioblastoma Multiforme (GBM) is characterized by high cancer cell heterogeneity and the presence of a complex tumor microenvironment. Those factors are a key obstacle for the treatment of this tumor type. To model the disease in mice, the current strategy is to grow GBM cells in serum-free non-adherent condition, which maintains their tumor-initiating potential. However, the so-generated tumors are histologically different from the one of origin. In this work, we performed high-throughput marker expression analysis and investigated the tumorigenicity of GBM cells enriched under different culture conditions. We identified a marker panel that distinguished tumorigenic sphere cultures from non-tumorigenic serum cultures (high CD56, SOX2, SOX9, and low CD105, CD248, αSMA). Contrary to previous work, we found that 'mixed cell cultures' grown in serum conditions are tumorigenic and express cancer stem cell (CSC) markers. As well, 1% serum plus bFGF and TGF-α preserved the tumorigenicity of sphere cultures and induced epithelial-to-mesenchymal transition gene expression. Furthermore, we identified 12 genes that could replace the 840 genes of The Cancer Genome Atlas (TCGA) used for GBM-subtyping. Our data suggest that the tumorigenicity of GBM cultures depend on cell culture strategies that retain CSCs in culture rather than the presence of serum in the cell culture medium.

• Foldager, Casper B.; Bendtsen, Michael; Berg, Lise C.; Brinchmann, Jan E.; Brittberg, Mats & Bunger, Cody [Vis alle 38 forfattere av denne artikkelen] (2016). Aarhus Regenerative Orthopaedics Symposium (AROS): Regeneration in the ageing population. Acta Orthopaedica. ISSN 1745-3674. 87, s. 1–5. doi: 10.1080/17453674.2017.1297918.
• Karlsen, Tommy Aleksander; De Souza, Gustavo Antonio; Ødegård, Bjørn; Engebretsen, Lars & Brinchmann, Jan E. (2016). microRNA-140 Inhibits Inflammation and Stimulates Chondrogenesis in a Model of Interleukin 1beta-induced Osteoarthritis. Molecular Therapy - Nucleic Acids. ISSN 2162-2531. 5. doi: 10.1038/mtna.2016.64.
• Costantini, Marco; Idaszek, Joanna; Szoke, Krisztina; Jaroszewicz, Jakub; Dentini, Mariella & Barbetta, Andrea [Vis alle 8 forfattere av denne artikkelen] (2016). 3D bioprinting of BM-MSCs-loaded ECM biomimetic hydrogels for in vitro neocartilage formation. Biofabrication. ISSN 1758-5082. 8(3). doi: 10.1088/1758-5090/8/3/035002.
• Behnan, Jinan; Stangeland, Biljana; Langella, Tiziana; Finocchiaro, Gaetano; Murrell, Wayne & Brinchmann, Jan E. (2016). Ultrasonic Surgical Aspirate is a Reliable Source for Culturing Glioblastoma Stem Cells. Scientific Reports. ISSN 2045-2322. 6. doi: 10.1038/srep32788. Fulltekst i vitenarkiv
• Aae, Tommy Frøseth; Randsborg, Per-Henrik; Breen, Anne Berg; Visnes, Håvard; Vindfeld, Søren & Sivertsen, Einar [Vis alle 10 forfattere av denne artikkelen] (2016). Norwegian Cartilage Project - a study protocol for a double-blinded randomized controlled trail comparing arthroscopic microfracture with debridement in focal cartilage defects in the knee. BMC Musculoskeletal Disorders. ISSN 1471-2474. 17:292. doi: 10.1186/s12891-016-1156-y.
• Szoke, Krisztina; Reinisch, Andreas; Østrup, Esben; Reinholt, Finn P. & Brinchmann, Jan E. (2016). Autologous cell sources in therapeutic vasculogenesis: In vitro and in vivo comparison of endothelial colony-forming cells from peripheral blood and endothelial cells isolated from adipose tissue. Cytotherapy. ISSN 1465-3249. 18(2), s. 242–252. doi: 10.1016/j.jcyt.2015.10.009.
• Randsborg, Per-Henrik; Brinchmann, Jan E.; Løken, Sverre Bertrand; Hanvold, Heidi Andreassen; Aae, Tommy Frøseth & Årøen, Asbjørn (2016). Focal cartilage defects in the knee -a randomized controlled trial comparing autologous chondrocyte implantation with arthroscopic debridement. BMC Musculoskeletal Disorders. ISSN 1471-2474. 17(1). doi: 10.1186/s12891-016-0969-z. Vis sammendrag

  Background: Focal cartilage injuries in the knee might have devastating effect due to the predisposition of early onset osteoarthritis. Various surgical treatment options are available, however no statistically significant differences have been found between the different surgical treatments. This supports the suggestion that the improvement might be a result of the post-operative rehabilitation rather than the surgery itself. Autologous chondrocyte implantation (ACI) has become a recognized treatment option for larger cartilage lesions in the knee. Although ACI has been compared to other surgical treatment such as microfracture and mosaicplasty, it has never been directly compared to simple arthroscopic debridement and rehabilitation alone. In this study we want to increase clinical and economic knowledge about autologous chondrocyte implantation compared to arthroscopic debridement and physical rehabilitation in the short and long run. Methods/Design: We will conduct a randomized controlled trial to compare ACI with simple arthroscopic debridement (AD) and physiotherapy for the treatment of cartilage lesions in the knee. The study will include a total of 82 patients, both men and non-pregnant women, with a full thickness cartilage defect in the weight bearing area of the femoral condyles or trochlea larger than 2 cm2. The lesion must be symptomatic, with a Lysholm score less than 75. The two treatment groups will receive identical rehabilitation protocol according to a modification of Wondrasch et al., which is an active rehabilitation and education program divided into 3 phases: accommodation, rehabilitation and return to activity. The patients will be followed for 24 months, with additional late follow-ups at 5 and 10 years to monitor the potential onset of osteoarthtitis. The primary outcome measure will be the difference in the KOOS knee-related quality of life (QoL) subscore in the ACI group compared to the AD group at 2 years. A combination of self-explanatory questionnaires, clinical parameters, clinical hop tests and radiographs and Magnetic Resonance Imaging (MRI) will be used as secondary endpoints. Discussion: This is the first study with a high level of evidence to compare ACI with simple debridement and physiotherapy for the treatment of isolated symptomatic full thickness lesions of the knee.

• Hart, Melanie L.; Izeta, Ander; Herrera-Imbroda, Bernardo; Amend, Bastian & Brinchmann, Jan E. (2015). Cell therapy for stress urinary incontinence. Tissue Engineering Part B: Reviews. ISSN 1937-3368. 21(4), s. 365–376. doi: 10.1089/ten.teb.2014.0627.
• Lilledahl, Magnus Borstad; Magnus Østgård, Olderøy; Finnøy, Andreas; Olstad, Kristin & Brinchmann, Jan E. (2015). Second harmonic generation imaging in tissue engineering and cartilage pathologies. Progress in Biomedical Optics and Imaging. ISSN 1605-7422. 9329. doi: 10.1117/12.2081583.
• Klein, Gerd; Hart, Melanie L.; Brinchmann, Jan E.; Rolauffs, Bernd; Stenzl, Arnulf & Sievert, Karl-Dietrich [Vis alle 7 forfattere av denne artikkelen] (2015). Mesenchymal stromal cells for sphincter regeneration. Advanced Drug Delivery Reviews. ISSN 0169-409X. 82, s. 123–136. doi: 10.1016/j.addr.2014.10.026.
• Pullisaar, Helen; Verket, Anders; Szoke, Krisztina; Tiainen, Hanna; Haugen, Håvard Jostein & Brinchmann, Jan E. [Vis alle 8 forfattere av denne artikkelen] (2015). Alginate hydrogel enriched with enamel matrix derivative to target osteogenic cell differentiation in TiO2 scaffolds. Journal of Tissue Engineering. ISSN 2041-7314. 6. doi: 10.1177/2041731415575870. Fulltekst i vitenarkiv
• Herlofsen, Sarah; Høiby, Torill; Cacchiarelli, Davide; Zhang, Xiaolan; Mikkelsen, Tarjei S. & Brinchmann, Jan E. (2014). Brief report: Importance of SOX8 for in vitro chondrogenic differentiation of human mesenchymal stromal cells. Stem Cells. ISSN 1066-5099. 32(6), s. 1629–1635. doi: 10.1002/stem.1642.
• Magnus Østgård, Olderøy; Lilledahl, Magnus Borstad; Beckwith, Marianne; Melvik, Jan Egil; Reinholt, Finn P. & Sikorski, Pawel [Vis alle 7 forfattere av denne artikkelen] (2014). Biochemical and Structural Characterization of Neocartilage Formed by Mesenchymal Stem Cells in Alginate Hydrogels. PLOS ONE. ISSN 1932-6203. 9(3). doi: 10.1371/journal.pone.0091662. Fulltekst i vitenarkiv
• Jakobsen, Rune Bruhn; Østrup, Esben; Xiaolan, Zhang; Mikkelsen, Tarjei S. & Brinchmann, Jan E. (2014). Analysis of the effects of five factors relevant to in vitro chondrogenesis of human mesenchymal stem cells using factorial design and high throughput mRNA-profiling. PLOS ONE. ISSN 1932-6203. 9(5). doi: 10.1371/journal.pone.0096615.
• Pullisaar, Helen; Reseland, Janne Elin; Haugen, Håvard Jostein; Brinchmann, Jan E. & Østrup, Esben (2014). Simvastatin coating of TiO2 scaffold induces osteogenic differentiation of human adipose tissue-derived mesenchymal stem cells. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 447(1), s. 139–144. doi: 10.1016/j.bbrc.2014.03.133.
• Karlsen, Tommy Aleksander; Jakobsen, Rune Bruhn; Mikkelsen, Tarjei S. & Brinchmann, Jan E. (2014). microRNA-140 Targets RALA and Regulates Chondrogenic Differentiation of Human Mesenchymal Stem Cells by Translational Enhancement of SOX9 and ACAN. Stem Cells and Development. ISSN 1547-3287. 23(3), s. 290–304. doi: 10.1089/scd.2013.0209.
• Vik-Mo, Einar O.; Nyakas, Marta Sølvi; Mikkelsen, Birthe Viftrup; Moe, Morten Carsten; Due-Tønnessen, Paulina & Suso, Else Marit Inderberg [Vis alle 16 forfattere av denne artikkelen] (2013). Therapeutic vaccination against autologous cancer stem cells with mRNA-transfected dendritic cells in patients with glioblastoma. Cancer Immunology and Immunotherapy. ISSN 0340-7004. 62(9), s. 1499–1509. doi: 10.1007/s00262-013-1453-3.
• Rohban, Rokhsareh; Reinisch, Andreas; Etchart, Nathalie; Schallmoser, Katharina; Hofmann, Nicole A. & Szoke, Krisztina [Vis alle 10 forfattere av denne artikkelen] (2013). Identification of an Effective Early Signaling Signature during Neo-Vasculogenesis In Vivo by Ex Vivo Proteomic Profiling. PLOS ONE. ISSN 1932-6203. 8(6). doi: 10.1371/journal.pone.0066909.
• Fernandes, Amilton Moreira; Herlofsen, Sarah; Karlsen, Tommy Aleksander; Kuchler, Axel Matthias; Fløisand, Yngvar & Brinchmann, Jan E. (2013). Similar Properties of Chondrocytes from Osteoarthritis Joints and Mesenchymal Stem Cells from Healthy Donors for Tissue Engineering of Articular Cartilage. PLOS ONE. ISSN 1932-6203. 8(5). doi: 10.1371/journal.pone.0062994.
• Karlsen, Tommy Aleksander & Brinchmann, Jan E. (2013). Liposome Delivery of MicroRNA-145 to Mesenchymal Stem Cells Leads to Immunological Off-target Effects Mediated by RIG-I. Molecular Therapy. ISSN 1525-0016. 21(6), s. 1169–1181. doi: 10.1038/mt.2013.55.
• Herlofsen, Sarah; Bryne, Jan Christian; Høiby, Torill; Wang, Li; Issner, Robbyn & Zhang, XL [Vis alle 13 forfattere av denne artikkelen] (2013). Genome-wide map of quantified epigenetic changes during in vitro chondrogenic differentiation of primary human mesenchymal stem cells. BMC Genomics. ISSN 1471-2164. 14. doi: 10.1186/1471-2164-14-105.
• Beitnes, Jan Otto; Øie, Erik; Shahdafar, Aboulghassem; Karlsen, Tommy Aleksander; Müller, Regine & Aakhus, Svend [Vis alle 8 forfattere av denne artikkelen] (2012). Intramyocardial Injections of Human Mesenchymal Stem Cells Following Acute Myocardial Infarction Modulate Scar Formation and Improve Left Ventricular Function. Cell Transplantation. ISSN 0963-6897. 21(8), s. 1697–1709. doi: 10.3727/096368911X627462.
• Szoke, Krisztina & Brinchmann, Jan E. (2012). Concise review: therapeutic potential of adipose tissue-derived angiogenic cells. Stem Cells Translational Medicine. ISSN 2157-6564. 1(9), s. 658–667. doi: 10.5966/sctm.2012-0069.
• Szoke, Krisztina; Beckstrøm, Karen Johanne & Brinchmann, Jan E. (2012). Human adipose tissue as a source of cells with angiogenic potential. Cell Transplantation. ISSN 0963-6897. 21(1), s. 235–250. doi: 10.3727/096368911X580518.
• Szoke, Krisztina; Beckstrøm, Karen Johanne & Brinchmann, Jan E. (2012). Ectopic expression of CDX4 in human mesenchymal stem cells does not affect HOX gene expression or induce hematopoietic reprogramming. Stem Cell Research. ISSN 1873-5061. 9(2), s. 135–142. doi: 10.1016/j.scr.2012.05.006.
• Beitnes, Jan Otto; Lunde, Ketil; Brinchmann, Jan E. & Aakhus, Svend (2011). Stem cells for cardiac repair in acute myocardial infarction. Expert Review of Cardiovascular Therapy. ISSN 1477-9072. 9(8), s. 1015–1025. doi: 10.1586/erc.11.108.
• Duggal, Shivali & Brinchmann, Jan E. (2011). Importance of Serum Source for the In Vitro Replicative Senescence of Human Bone Marrow Derived Mesenchymal Stem Cells. Journal of Cellular Physiology. ISSN 0021-9541. 226(11), s. 2908–2915. doi: 10.1002/jcp.22637.
• Karlsen, Tommy Aleksander; Shahdafar, Aboulghassem & Brinchmann, Jan E. (2011). Human Primary Articular Chondrocytes, Chondroblasts-Like Cells, and Dedifferentiated Chondrocytes: Differences in Gene, MicroRNA, and Protein Expression and Phenotype. Tissue Engineering, Parts A, B, & C. ISSN 2152-4947. 17(2), s. 219–227. doi: 10.1089/ten.tec.2010.0200.
• Gordeladze, Jan Oxholm; Reseland, Janne Elin; Karlsen, Tommy Aleksander; Jakobsen, Rune Bruhn; Engebretsen, Lars & Lyngstadaas, Ståle Petter [Vis alle 9 forfattere av denne artikkelen] (2011). Bone and Cartilage from Stem Cells: Growth Optimalization and Stabilization of Cell Phenotypes. I Eberli, Daniel (Red.), Bone and Cartilage from Stem Cells: Growth Optimalization and Stabilization of Cell Phenotypes. IntechOpen. ISSN 978-953-307-663-8. s. 23–56. doi: 10.5772/19826.
• Herlofsen, Sarah; Kuechler, Axel Matthias; Melvik, Jan Egil & Brinchmann, Jan E. (2011). Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells in Self-Gelling Alginate Discs Reveals Novel Chondrogenic Signature Gene Clusters. Tissue Engineering. ISSN 1076-3279. 17(7-8), s. 1003–1013. doi: 10.1089/ten.tea.2010.0499.
• Karlsen, Tommy Aleksander; Mirtaheri, Peyman; Shahdafar, Aboulghassem; Fløisand, Yngvar & Brinchmann, Jan E. (2011). Effect of Three-Dimensional Culture and Incubator Gas Concentration on Phenotype and Differentiation Capability of Human Mesenchymal Stem Cells. Journal of Cellular Biochemistry. ISSN 0730-2312. 112(2), s. 684–693. doi: 10.1002/jcb.22978.
• Jakobsen, Rune Bruhn; Shahdafar, Aboulghassem; Reinholt, Finn P. & Brinchmann, Jan E. (2010). Chondrogenesis in a hyaluronic acid scaffold: comparison between chondrocytes and MSC from bone marrow and adipose tissue. Knee Surgery, Sports Traumatology, Arthroscopy. ISSN 0942-2056. 18(10), s. 1407–1416. doi: 10.1007/s00167-009-1017-4.
• Duggal, Shivali; Frønsdal, Katrine; Szoke, Krisztina; Shahdafar, Aboulghassem; Melvik, Jan Egil & Brinchmann, Jan E. (2009). Phenotype and Gene Expression of Human Mesenchymal Stem Cells in Alginate Scaffolds. Tissue Engineering. ISSN 1076-3279. 15(7), s. 1763–1773. doi: 10.1089/ten.tea.2008.0306.
• Beitnes, Jan Otto; Halvorsen, Bente ; Hopp, Einar; Lunde, Ketil; Solheim, Svein & Arnesen, Harald [Vis alle 9 forfattere av denne artikkelen] (2009). Long-term results after intracoronary injection of autologous mononuclear bone marrow cells in acute myocardial infarction: the ASTAMI randomised, controlled study. Heart. ISSN 1355-6037. 95(24), s. 1983–1989. doi: 10.1136/hrt.2009.178913.
• Hildrestrand, Gunn Annette; Duggal, Shivali; Bjørås, Magnar; Luna, Luisa & Brinchmann, Jan E. (2009). Modulation of DNA glycosylase activities in mesenchymal stem cells. Experimental Cell Research. ISSN 0014-4827. 315(15), s. 2558–2567. doi: 10.1016/j.yexcr.2009.05.017.
• Wester, T; Jørgensen, Jørgen J.; Stranden, Einar; Tønnfjord, Geir E.; Bay, Dag & Sandbæk, Gunnar [Vis alle 9 forfattere av denne artikkelen] (2008). Treatment with autologous mononuclear cells in patients with critical lower limb ischemia. A pilot study. Scandinavian Journal of Surgery. ISSN 1457-4969. 97, s. 56–62.
• Wester, T; Jorgensen, JJ; Stranden, E; Sandbaek, G; Tjonnfjord, G & Bay, D [Vis alle 9 forfattere av denne artikkelen] (2008). Treatment with autologous bone marrow mononuclear cells in patients with critical lower limb ischaemia. A pilot study. Scandinavian Journal of Surgery. ISSN 1457-4969. 97.
• Løken, Sverre Bertrand; Jakobsen, Rune Bruhn; Årøen, Asbjørn; Heir, Stig; Shahdafar, Aboulghassem & Brinchmann, Jan E. [Vis alle 8 forfattere av denne artikkelen] (2008). Bone marrow mesenchymal stem cells in a hyaluronan scaffold for treatment of an osteochondral defect in a rabbit model. Knee Surgery, Sports Traumatology, Arthroscopy. ISSN 0942-2056. 16, s. 896–903. doi: 10.1007/s00167-008-0566-2.
• Dahl, John Arne; Duggal, Shivali; Coulston, N; Millar, D; Melki, J & Shahdafar, Aboulghassem [Vis alle 8 forfattere av denne artikkelen] (2008). Genetic and epigenetic instability of human bone marrow mesenchymal stem cells expanded in autologous serum or fetal bovine serum. International Journal of Developmental Biology. ISSN 0214-6282. 52(8), s. 1033–1042. doi: 10.1387/ijdb.082663jd.
• Wester, Torjus; Jørgensen, Jørgen J.; Stranden, Einar; Sandbæk, Gunnar; Tjønnfjord, G & Bay, Dag [Vis alle 9 forfattere av denne artikkelen] (2008). Treatment with autologous mononuclear cells in patients with critical lower limb ischaemia. A pilot study. Scandinavian Journal of Surgery. ISSN 1457-4969. 97, s. 56–62. Vis sammendrag

  Background and Aims: Treatment with autologous, bone marrow mononuclear stem cells has shown effects in patients with chronic limb ischaemia in one randomized clinical study. The aim of the study was to test the potential effect of stem cell treatment in a strict defined group of patients with stable critical limb ischaemia (cli). design: a prospective, combined-centre pilot study. Material: eight patients with cli of the lower extremities, and without any other treatment options. Methods: bone marrow cells were harvested from the patient’s iliac crest and, after separation, injected into the calf muscles of the affected leg. outcome was evaluated by digital subtraction angiography (dsa), visual analogue scale (Vas) and several non-invasive circulatory physiological tests. Results: There were no complications from the procedures. Two patients were amputated two months after cell injection. Five patients reported pain relief after four months. Five patients could be evaluated at eight months. according to Vas and physiological tests, they were all either stable or showed improvement. Conclusion: This method seems to be a safe option for treating patients with cli. inclusion of patients took a long time, mainly because many patients with cli are offered endovascular treatment in our institution. while symptomatic improvement was found in individual patients, larger trials are required to investigate efficacy. This will probably require multi-centre participation.

• Shahdafar, Aboulghassem; Løken, Sverre Bertrand; Dahl, John Arne; Tunheim, Siv Haugen; Collas, Philippe & Reinholt, Finn P. [Vis alle 8 forfattere av denne artikkelen] (2008). Persistence of Collagen Type II Synthesis and Secretion in Rapidly Proliferating Human Articular Chondrocytes In Vitro. Tissue Engineering. ISSN 1076-3279. 14(12), s. 1999–2007. doi: 10.1089/ten.tea.2007.0344.
• Brinchmann, Jan E. (2008). Expanding autologous multipotent mesenchymal bone marrow stromal cells. Journal of the Neurological Sciences. ISSN 0022-510X. 265. doi: 10.1016/j.jns.2007.05.006.
• Lunde, Ketil; Solheim, S; Aakhus, Svend; Arnesen, Harald; Abdelnoor, M & Egeland, T [Vis alle 20 forfattere av denne artikkelen] (2006). Intracoronary injection of mononuclear bone marrow cells in acute myocardial infarction. New England Journal of Medicine. ISSN 0028-4793. 355(12), s. 1199–1209.
• Boquest, Andrew Craig; Shahdafar, Aboulghassem; Brinchmann, Jan E. & Collas, Philippe (2006). Isolation of stromal stem cells from human adipose tissue. Methods in molecular biology. ISSN 1064-3745. s. 35–46.
• Kvale, Espen Østhagen; Dalgaard, Jakob; Lund-Johansen, Fridtjof; Rollag, Halvor; Farkas, Lorant & Midtvedt, Karsten [Vis alle 9 forfattere av denne artikkelen] (2006). CD11c+ dendritic cells and plasmacytoid DCs are activated by human cytomegalovirus and retain efficient T cell–stimulatory capability upon infection. Blood. ISSN 0006-4971. 107(5), s. 2022–2029. doi: 10.1182/blood-2005-05-2016.
• Dalgaard, Jakob; Beckstrøm, Karen Johanne; Jahnsen, Frode Lars & Brinchmann, Jan E. (2005). Differential capability for phagocytosis of apoptotic and necrotic leukaemia cells by human peripheral blood dendritic cell subsets. Journal of Leukocyte Biology. ISSN 0741-5400. 77, s. 689–698.
• Boquest, Andrew Craig; Shahdafar, A; Frønsdal, Katrine; Sigurjonsson, Olaur E.; Tunheim, S.H. & Collas, Philippe [Vis alle 7 forfattere av denne artikkelen] (2005). Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture. Molecular Biology of the Cell. ISSN 1059-1524. 16, s. 1131–1141.
• Shahdafar, Aboulghassem; Frønsdal, Katrine; Haug, Terje; Reinholt, Finn P. & Brinchmann, Jan E. (2005). In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression and trascriptome stability. Stem Cells. ISSN 1066-5099. 23, s. 1357–1366.
• Hedfors, Ida Aagård; Beckstrøm, Karen Johanne; Benati, Claudia; Bonini, Chiara & Brinchmann, Jan E. (2005). Retrovirus mediated gene transduction of human T-cell subsets. Cancer Immunology and Immunotherapy. ISSN 0340-7004. 54, s. 759–768. doi: 10.1007/s00262-004-0647-0.
• Hedfors, Ida Aagård & Brinchmann, Jan E. (2004). Polyclonal T-cell activation protocol stimulates preferential expansion of EBV-specific T-cell clones in vitro. Cancer Immunology and Immunotherapy. ISSN 0340-7004. 58(5), s. 522–532.
• Spurkland, Anne; Brinchmann, Jan E.; Markussen, Gunnar; Pedeutour, Florence; Munthe, Else & Lea, Tor [Vis alle 9 forfattere av denne artikkelen] (1998). Molecular cloning of a T cell-specific adapter protein (TSAd) containing an Src homology (SH) 2 domain and putative SH3 and phosphotyrosine binding sites. Journal of Biological Chemistry. ISSN 0021-9258. doi: 10.1074/jbc.273.8.4539.

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• Aae, Tommy Frøseth; Karlsen, Tommy Aleksander; Bos-Haugen, Ida Kristin; Risberg, May Arna; Lian, Øystein Bjerkestrand & Brinchmann, Jan E. (2020). Evaluating plasma extracellular vesicle microRNAs as possible biomarkers for osteoarthritis. Osteoarthritis and Cartilage Open. ISSN 2665-9131. 1. doi: 10.1016/j.ocarto.2019.100018.
• Årøen, Asbjørn; Kjennvold, Stian; Brinchmann, Jan E.; Randsborg, Per-Henrik; Reinholt, Finn P. & Engebretsen, Lars (2019). Bone marrow derived mesenchymal stem cells in a human clinical trial to repair articular cartilage defects of the knee .
• Årøen, Asbjørn; Kjennvold, Stian; Brinchmann, Jan E.; Randsborg, Per-Henrik; Reinholt, Finn P. & Engebretsen, Lars (2018). Bone marrow derived mesenchymal stem cells in a human clinical trial to repair articular cartilage defects of the knee .
• Karlsen, Tommy Aleksander; Jakobsen, Rune Bruhn & Brinchmann, Jan E. (2017). Early molecular events during in vitro chondrogenesisi. I Atkinson, Kerry (Red.), The biology and therapeutic Application of mesenchymal cells. Wiley-Blackwell. ISSN 978-1-118-90751-1. s. 167–190.
• Jakobsen, Rune Bruhn; Shahdafar, Aboulghassem; Reinholt, Finn P. & Brinchmann, Jan E. (2014). Erratum to Chondrogenesis in a hyaluronic acid scaffold: Comparison between chondrocytes and MSC from bone marrow and adipose tissue (Knee Surg Sports Traumatol Arthrosc, (2010), 18, (1407-1416), 10.1007/s00167-009-1017-4). Knee Surgery, Sports Traumatology, Arthroscopy. ISSN 0942-2056. 22(7), s. 1711–1714. doi: 10.1007/s00167-014-2938-0.
• Pullisaar, Helen; Verket, Anders; Szoke, Krisztina; Tiainen, Hanna; Haugen, Håvard Jostein & Brinchmann, Jan E. [Vis alle 8 forfattere av denne artikkelen] (2014). Extracellular matrix coated ceramic scaffold enhance osteoblast differentiation.
• Olderøy, Magnus Østgård; Beckwith, Marianne; Rrinholt, F P; Lilledahl, Magnus Borstad; Sikorski, Pawel & Brinchmann, Jan E. (2013). Identification of functional structures in tissue engineered cartilage: from nm to macro scale. European Cells and Materials. ISSN 1473-2262. 26, s. 23–23.
• Østrup, Esben; Tiainen, Hanna; Reseland, Janne Elin; Melvik, Jan Egil; Lyngstadaas, Ståle Petter & Brinchmann, Jan E. (2012). Improving conditions for MSC growth on porous TiO2 scaffolds. Journal of Tissue Engineering and Regenerative Medicine. ISSN 1932-6254. 6, s. 234–234.
• Beitnes, Jan Otto; Øie, Erik; Shahdafar, Aboulghassem; Karlsen, Tommy Aleksander; Aakhus, Svend & Brinchmann, Jan E. (2010). Mesenchymal stem cells from skeletal muscle and adipose tissue improve LV function after myocardial infarction, but do not differentiate into cardiomyocytes. European Heart Journal. ISSN 0195-668X. 31, s. 397–397.
• Beitnes, JO; Hopp, Einar; Lunde, Ketil; Smith, Hans-Jørgen; Solheim, Svein & Arnesen, Harald [Vis alle 9 forfattere av denne artikkelen] (2008). Long-term follow-up of left ventricular function after acute myocardial infarction treated with intracoronary injection of autologous bone marrow cells. The ASTAMI Study.
• Wester, Torjus; Stranden, Einar; Jørgensen, Jørgen J.; Kroese, Andries Jan; Kazmi, SS & Sandbæk, Gunnar [Vis alle 10 forfattere av denne artikkelen] (2005). Autologe stamceller i behandlingen av pasienter med kritisk iskemi – en pilotstudie.
• Fernandes, Amilton; Brinchmann, Jan E. & Engebretsen, Lars (2013). Tissue engineering of hyaline cartilage. A comparison between articular chondrocytes and mesenchymal stem cells. Universitetet i Oslo.
• Duggal, Shivali & Brinchmann, Jan E. (2010). Human mesenchymal stem cells: Culture conditions, genomic and epigenetic stability and senescence. Unipub forlag. ISSN 978-82-8072-555-4.
• Hedfors, Ida Aagård & Brinchmann, Jan E. (2007). Activation and gene transduction of human T cells. Unipub forlag. ISSN 978-82-8072-677-3.
• Shahdafar, Aboulghassem & Brinchmann, Jan E. (2007). Human somatic cells in regenerative medicine : in vitro characterization of mesenchymal stem cells and chondrocytes. Unipub forlag. ISSN 978-82-8072-740-4.

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