May-Kristin Torp

• Torp, May-Kristin; Ranheim, Trine; Schjalm, Camilla; Hjorth, Marit; Heiestad, Christina Mathisen & Dalen, Knut Tomas [Vis alle 13 forfattere av denne artikkelen] (2022). Intracellular Complement Component 3 Attenuated Ischemia-Reperfusion Injury in the Isolated Buffer-Perfused Mouse Heart and Is Associated With Improved Metabolic Homeostasis. Frontiers in Immunology. ISSN 1664-3224. 13. doi: 10.3389/fimmu.2022.870811.
• Li, Yuchuan; Torp, May-Kristin; Norheim, Frode; Khanal, Prabhat; Kimmel, Alan R. & Stensløkken, Kåre-Olav [Vis alle 8 forfattere av denne artikkelen] (2020). Isolated Plin5-deficient cardiomyocytes store less lipid droplets than normal, but without increased sensitivity to hypoxia . Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. ISSN 1388-1981. 1866(4). doi: 10.1016/j.bbalip.2020.158873. Fulltekst i vitenarkiv Vis sammendrag

  Plin5 is abundantly expressed in the heart where it binds to lipid droplets (LDs) and facilitates physical interaction between LDs and mitochondria. We isolated cardiomyocytes from adult Plin5+/+ and Plin5-/- mice to study the role of Plin5 for fatty acid uptake, LD accumulation, fatty acid oxidation, and tolerance to hypoxia. Cardiomyocytes isolated from Plin5-/- mice cultured with oleic acid stored less LDs than Plin5+/+, but comparable levels to Plin5+/+ cardiomyocytes when adipose triglyceride lipase activity was inhibited. The ability to oxidize fatty acids into CO2 was similar between Plin5+/+ and Plin5-/- cardiomyocytes, but Plin5-/- cardiomyocytes had a transient increase in intracellular fatty acid oxidation intermediates. After pre-incubation with oleic acids, Plin5-/- cardiomyocytes retained a higher content of glycogen and showed improved tolerance to hypoxia compared to Plin5+/+. In isolated, perfused hearts, deletion of Plin5 had no important effect on ventricular pressures or infarct size after ischemia. Old Plin5-/- mice had reduced levels of cardiac triacylglycerides, increased heart weight, and apart from modest elevated expression of mRNAs for beta myosin heavy chain Myh7 and the fatty acid transporter Cd36, other genes involved in fatty acid oxidation, glycogen metabolism and glucose utilization were essentially unchanged by removal of Plin5. Plin5 seems to facilitate cardiac LD storage primarily by repressing adipose triglyceride lipase activity without altering cardiac fatty acid oxidation capacity. Expression of Plin5 and cardiac LD content of isolated cardiomyocytes has little importance for tolerance to acute hypoxia and ischemia, which contrasts the protective role for Plin5 in mouse models during myocardial ischemia.

• Mariero, Lars Henrik; Torp, May-Kristin; Heiestad, Christina Mathisen; Baysa, Anton; Li, Yuchuan & Valen, Guro [Vis alle 8 forfattere av denne artikkelen] (2019). Inhibiting nucleolin reduces inflammation induced by mitochondrial DNA in cardiomyocytes exposed to hypoxia and reoxygenation. British Journal of Pharmacology. ISSN 0007-1188. 176(22), s. 4360–4372. doi: 10.1111/bph.14830. Fulltekst i vitenarkiv
• Torp, May-Kristin; Yang, Kuan; Ranheim, Trine; Lauritzen, Knut Husø; Alfsnes, Katrine & Vinge, Leif Erik [Vis alle 10 forfattere av denne artikkelen] (2019). Mammalian target of rapamycin (mTOR) and the proteasome attenuates IL-1β expression in primary mouse cardiac fibroblasts. Frontiers in Immunology. ISSN 1664-3224. 10. doi: 10.3389/fimmu.2019.01285. Fulltekst i vitenarkiv
• Lefevre, Sjannie; Stecyk, Jonathan Anthony William; Torp, May-Kristin; Løvold, Lisa Yuen; Sørensen, Christina & Johansen, Ida Beitnes [Vis alle 10 forfattere av denne artikkelen] (2017). Re-oxygenation after anoxia induces brain cell death and memory loss in the anoxia-tolerant crucian carp. Journal of Experimental Biology. ISSN 0022-0949. 220(21), s. 3883–3895. doi: 10.1242/jeb.165118. Fulltekst i vitenarkiv
• Sokolova, Marina; Vinge, Leif Erik; Alfsnes, Katrine; Olsen, Maria Belland; Eide, Lars & Kaasbøll, Ole Jørgen [Vis alle 16 forfattere av denne artikkelen] (2017). Palmitate promotes inflammatory responses and cellular senescence in cardiac fibroblasts. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. ISSN 1388-1981. 1862(2), s. 234–245. doi: 10.1016/j.bbalip.2016.11.003.
• Bliksøen, Marte; Mariero, Lars Henrik; Torp, May-Kristin; Baysa, Anton; Ytrehus, Kirsti & Haugen, Fred [Vis alle 10 forfattere av denne artikkelen] (2016). Extracellular mtDNA activates NF-κB via toll-like receptor 9 and induces cell death in cardiomyocytes. Basic Research in Cardiology. ISSN 0300-8428. 111(42). doi: 10.1007/s00395-016-0553-6.

Se alle arbeider i Cristin

• Lefevre, Sjannie; Stecyk, Jonathan A. W.; Torp, May-Kristin & Nilsson, Göran Erik (2014). HOW TOLERANT IS AN ANOXIA-TOLERANT VERTEBRATE? Vis sammendrag

  The crucian carp Carassius carassius is capable of surviving without oxygen for several months, by carefully matching ATP supply and demand. It does so by exploiting its unique ability to produce ethanol as an anaerobic end-product and undergo partial metabolic rate suppression, shutting down non-vital functions such as vision. Even so, it is unknown if crucian carp are fully able to protect themselves from the damage (i.e. cell death), normally caused by absence and particularly re-entrance of oxygen. Here, we investigated the occurrence of apoptosis in brain of crucian carp exposed to normoxia, anoxia and re-oxygenation in the laboratory, using Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). We also measured mRNA and protein levels of the apoptosis executer protein Caspase 3 using quantitative real-time PCR and Western blotting. Additionally, Caspase 3 expression levels were measured in brains from crucian carp captured in their natural habitat at different months of the year. Overall, crucian carp appear to maintain apoptotic activity in anoxia, but there is a transient increase in the early re-oxygenation phase. In its natural habitat transcription of Caspase 3 is generally much down-regulated during the winter, but protein levels are maintained, with only a slight increase in the spring.

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