Viola Lobert

• Hurem, Selma; Gomes, Tania; Brede, Dag Anders; Mayer, Ian; Lobert, Viola; Mutoloki, Stephen; Gutzkow, Kristine Bjerve; Teien, Hans-Christian; Oughton, Deborah Helen; Alestrøm, Peter & Lyche, Jan Ludvig (2018). Gamma irradiation during gametogenesis in young adult zebrafish causes persistent genotoxicity and adverse reproductive effects. Ecotoxicology and Environmental Safety.  ISSN 0147-6513.  154, s 19- 26 . doi: 10.1016/j.ecoenv.2018.02.031 Full text in Research Archive. Show summary

  The biological effects of gamma radiation may exert damage beyond that of the individual through its deleterious effects on reproductive function. Impaired reproductive performance can result in reduced population size over consecutive generations. In a continued effort to investigate reproductive and heritable effects of ionizing radiation, we recently demonstrated adverse effects and genomic instability in progeny of parents exposed to gamma radiation. In the present study, genotoxicity and effects on the reproduction following subchronic exposure during a gametogenesis cycle to 60Co gamma radiation (27 days, 8.7 and 53 mGy/h, total doses 5.2 and 31 Gy) were investigated in the adult wild-type zebrafish (Danio rerio). A significant reduction in embryo production was observed one month after exposure in the 53 mGy/h exposure group compared to control and 8.7 mGy/h. One year later, embryo production was significantly lower in the 53 mGy/h group compared only to control, with observed sterility, accompanied by a regression of reproductive organs in 100% of the fish 1.5 years after exposure. Histopathological examinations revealed no significant changes in the testis in the 8.7 mGy/h group, while in 62.5% of females exposed to this dose rate the oogenesis was found to be only at the early previtellogenic stage. The DNA damage determined in whole blood, 1.5 years after irradiation, using a high throughput Comet assay, was significantly higher in the exposed groups (1.2 and 3-fold increase in 8.7 and 53 mGy/h females respectively; 3-fold and 2-fold increase in 8.7 and 53 mGy/h males respectively) compared to controls. A significantly higher number of micronuclei (4–5%) was found in erythrocytes of both the 8.7 and 53 mGy/h fish compared to controls. This study shows that gamma radiation at a dose rate of ≥ 8.7 mGy/h during gametogenesis causes adverse reproductive effects and persistent genotoxicity (DNA damage and increased micronuclei) in adult zebrafish.

• O'Farrell, Fergal; Lobert, Viola; Sneeggen, Marte; Jain, Ashish; Katheder, Nadja Sandra; Wenzel, Eva; Schultz, Sebastian; Tan, Kia Wee; Brech, Andreas; Stenmark, Harald Alfred & Rusten, Tor Erik (2017). Class III phosphatidylinositol-3-OH kinase controls epithelial integrity through endosomal LKB1 regulation. Nature Cell Biology.  ISSN 1465-7392.  19, s 1412- 1423 . doi: 10.1038/ncb3631
• Halsne, Ruth; Tandberg, Julia Isabel; Lobert, Viola; Østby, Gunn Charlotte; Thoen, Even; Ropstad, Erik & Verhaegen, Steven (2016). Effects of perfluorinated alkyl acids on cellular responses of MCF-10A mammary epithelial cells in monolayers and on acini formation in vitro. Toxicology Letters.  ISSN 0378-4274.  259(30), s 95- 107 . doi: 10.1016/j.toxlet.2016.08.004
• Holland, Petter; Knævelsrud, Helene; Søreng, Kristiane; Mathai, Benan John; Lystad, Alf Håkon; Pankiv, Serhiy; Bjørndal, Gunnveig Toft; Schultz, Sebastian; Lobert, Viola; Chan, Robin B; Zhou, Bowen; Liestøl, Knut; Carlsson, Sven R; Melia, Thomas J; Di Paolo, Gilbert & Simonsen, Anne (2016). HS1BP3 negatively regulates autophagy by modulation of phosphatidic acid levels. Nature Communications.  ISSN 2041-1723.  7 . doi: 10.1038/ncomms13889 Full text in Research Archive.
• Lobert, Viola; Mouradov, Dmitri & Heath, Joan K. (2016). Focusing the Spotlight on the Zebrafi sh Intestine to Illuminate Mechanisms of Colorectal Cancer. Advances in Experimental Medicine and Biology.  ISSN 0065-2598.  916, s 411- 437
• Oppelt, Angela; Haugsten, Ellen Margrethe; Zech, Tobias; Danielsen, Håvard Emil; Sveen, Anita; Lobert, Viola Helene; Skotheim, Rolf Inge & Wesche, Jørgen (2014). PIKfyve, MTMR3 and their product PtdIns5P regulate cancer cell migration and invasion through activation of Rac1. Biochemical Journal.  ISSN 0264-6021.  461(3), s 383- 390 . doi: 10.1042/BJ20140132
• Oppelt, Angela; Lobert, Viola Helene; Haglund, Kaisa; Mackey, Ashley M.; Rameh, Lucia E.; Liestøl, Knut; Schink, Kay Oliver; Pedersen, Nina Marie; Wenzel, Eva; Haugsten, Ellen Margrethe; Brech, Andreas; Rusten, Tor Erik; Stenmark, Harald Alfred & Wesche, Jørgen (2013). Production of phosphatidylinositol 5-phosphate via PIKfyve and MTMR3 regulates cell migration. EMBO Reports.  ISSN 1469-221X.  14(1), s 57- 64 . doi: 10.1038/embor.2012.183
• Lobert, Viola Helene & Stenmark, Harald Alfred (2012). The ESCRT machinery mediates polarization of fibroblasts through regulation of myosin light chain. Journal of Cell Science.  ISSN 0021-9533.  125(1), s 29- 36 . doi: 10.1242/jcs.088310
• Lobert, Viola Helene & Stenmark, Harald (2011). Cell Polarity and Migration: Emerging Role for the Endosomal Sorting Machinery. Physiology.  ISSN 1548-9213.  26(3), s 171- 180 . doi: 10.1152/physiol.00054.2010
• Malerød, Lene; Pedersen, Nina Marie; Wegner, Catherine E Sem; Lobert, Viola Helene; Leithe, Edward; Brech, Andreas; Rivedal, Edgar; Liestøl, Knut & Stenmark, Harald (2011). Cargo-Dependent Degradation of ESCRT-I as a Feedback Mechanism to Modulate Endosomal Sorting. Traffic : the International Journal of Intracellular Transport.  ISSN 1398-9219.  12(9), s 1211- 1226 . doi: 10.1111/j.1600-0854.2011.01220.x
• Lobert, Viola Helene; Brech, Andreas; Pedersen, Nina Marie; Wesche, Jørgen; Oppelt, Angela; Malerød, Lene & Stenmark, Harald (2010). Ubiquitination of alpha 5 beta 1 lntegrin Controls Fibroblast Migration through Lysosomal Degradation of Fibronectin-lntegrin Complexes. Developmental Cell.  ISSN 1534-5807.  19(1), s 148- 159 . doi: 10.1016/j.devcel.2010.06.010
• Rodahl, Lina; Stuffers, Susanne; Lobert, Viola Helene & Stenmark, Harald Alfred (2009). The role of ESCRT proteins in attenuation of cell signalling. Biochemical Society Transactions.  ISSN 0300-5127.  37, s 137- 142 . doi: 10.1042/BST0370137

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• Griffiths, Gareth Wyn; Müller, Ference; Ledin, Johan; Patton, E. Elizabeth; Gjøen, Tor; Lobert, Viola; Winther-Larsen, Hanne Cecilie; Mullins, Mary; Joly, Jean-Stephane; Weltzien, Finn-Arne; Press, Charles McLean & Alestrøm, Peter (2016). Fish from Head to Tail: The 9th European Zebrafish Meeting in Oslo. Zebrafish.  ISSN 1545-8547.  13(2), s 132- 137 . doi: 10.1089/zeb.2015.1212
• Hurem, Selma; Gomes, Tania; Brede, Dag Anders; Hansen, Elisabeth Lindbo; Lobert, Viola; Martin, Leonardo M; Mayer, Ian; Mutoloki, Stephen; Salbu, Brit; Teien, Hans-Christian; Oughton, Deborah Helen; Aleström, Peter & Lyche, Jan Ludvig (2016). Gamma radiation causes adverse reproductive and heritable effects in zebrafish.
• Lobert, Viola Helene; Bruun, Jarle; Abrahamsen, Hilde; Lothe, Ragnhild A; Stenmark, Harald Alfred; Kolberg, Matthias & Campsteijn, Coen (2013). Antibody crossreactivity between the tumour suppressor PHLPP1 and the proto-oncogene beta-catenin. EMBO Reports.  ISSN 1469-221X.  14(1), s 10- 11 . doi: 10.1038/embor.2012.188

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