Justyna Polak

• Polak, Justyna; Wagnerberger, Johanna Henny; Torsetnes, Silje Bøen; Lindeman, Ida; Høglund, Rune Alexander Aamodt & Vartdal, Frode [Vis alle 8 forfattere av denne artikkelen] (2023). Single-cell transcriptomics combined with proteomics of intrathecal IgG reveal transcriptional heterogeneity of oligoclonal IgG-secreting cells in multiple sclerosis. Frontiers in Cellular Neuroscience. ISSN 1662-5102. 17. doi: 10.3389/fncel.2023.1189709. Fulltekst i vitenarkiv
• Lindeman, Ida; Polak, Justyna; Qiao, Shuo-Wang; Holmøy, Trygve; Høglund, Rune Alexander & Vartdal, Frode [Vis alle 9 forfattere av denne artikkelen] (2022). Stereotyped B-cell responses are linked to IgG constant region polymorphisms in multiple sclerosis. European Journal of Immunology. ISSN 0014-2980. 52(4), s. 550–565. doi: 10.1002/eji.202149576. Vis sammendrag

  Clonally related B cells infiltrate the brain, meninges, and cerebrospinal fluid of MS patients, but the mechanisms driving the B-cell response and shaping the immunoglobulin repertoires remain unclear. Here, we used single-cell full-length RNA-seq and BCR reconstruction to simultaneously assess the phenotypes, isotypes, constant region polymorphisms, and the paired heavy- and light-chain repertoires in intrathecal B cells. We detected extensive clonal connections between the memory B cell and antibody-secreting cell (ASC) compartments and observed clonally related cells of different isotypes including IgM/IgG1, IgG1/IgA1, IgG1/IgG2, and IgM/IgA1. There was a strong dominance of the G1m1 allotype constant region polymorphisms in ASCs, but not in memory B cells. Tightly linked to the G1m1 allotype, we found a preferential pairing of the immunoglobulin heavy-chain variable (IGHV)4 gene family with the κ variable (IGKV)1 gene family. The IGHV4-39 gene was most used and showed the highest frequency of pairing with IGKV1-5 and IGKV1(D)-33. These results link IgG constant region polymorphisms to stereotyped B-cell responses in MS and indicate that the intrathecal B-cell response in these patients could be directed against structurally similar epitopes

• Lindeman, Ida; Zhou, Chunyan; Eggesbø, Linn Margrethe; Miao, Zhichao; Polak, Justyna & Lundin, Knut Erik Aslaksen [Vis alle 10 forfattere av denne artikkelen] (2021). Longevity, clonal relationship, and transcriptional program of celiac disease-specific plasma cells. Journal of Experimental Medicine (JEM). ISSN 0022-1007. 218:e20200852(2), s. 1–16. doi: 10.1084/jem.20200852. Fulltekst i vitenarkiv
• Høglund, Rune Alexander; Polak, Justyna; Vartdal, Frode; Holmøy, Trygve & Lossius, Peter Andreas Vold (2018). B-cell composition in the blood and cerebrospinal fluid of multiple sclerosis patients treated with dimethyl fumarate. Multiple Sclerosis and Related Disorders. ISSN 2211-0348. 26, s. 90–95. doi: 10.1016/j.msard.2018.08.032. Fulltekst i vitenarkiv

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