Genetic modification may both decrease and increase containment level. Work with eg. virus must be risk-assessed for additional containment measures before use. All laboratories at NCMM are locked with restricted access. General safety precautions and containment measures for laboratory levels 1 and 2 are valid.
Disposal
Types of waste
Vector cultures with resistant bacteria, yeast, cell lines in culture and lentiviral vectors. All volumes below 10 liters.
Storage
The waste is stored in yellow plastic boxes in the locked waste-room. The boxes, in polypropylene, are labeled biological waste and sealed with 'click on all around " and glue in the lid, when closed.
Transportation
Biological waste is picked up once a week for direct incineration.
Procedures for handling
All waste is inactivated by the following procedures:
- Autoclaving for 1 hour at 121 degrees before it is poured out. NCMM has access for autoclaving.
- Disinfection using Virkon 1 -2% over-night before it is poured out.
- Directly sent for incineration.
- All waste at level 2 shall be inactivated before it is sent for incineration.
Referances
Laws and regulation (in Norwegian)
- Forskrift om innesluttet bruk av genmodifiserte mikroorganismer
- Forskrift om innesluttet bruk av genmodifiserte dyr (dyreforskriften)
- Forskrift om bestemte former for undervisningsvirksomhet som innebærer innesluttet bruk av genmodifiserte mikroorganismer
- Forskrift om innesluttet bruk av genmodifiserte planter (planteforskriften)