Artur Cieslar-Pobuda

• Caglayan, Safak; Hashim, Adnan; Cieslar-Pobuda, Artur; Jensen, Vidar; Behringer, Sidney; Talug, Burcu; Chu, Dinh Toi; Pecquet, Christian; Rogne, Marie; Brech, Andreas; Brorson, Sverre Henning; Nagelhus, Erlend Arnulf; Hannibal, Luciana; Boschi, Antonella; Tasken, Kjetil & Staerk, Judith (2020). Optic Atrophy 1 Controls Human Neuronal Development by Preventing Aberrant Nuclear DNA Methylation. iScience.  ISSN 2589-0042.  23(6) . doi: 10.1016/j.isci.2020.101154 Full text in Research Archive. Show summary

  Optic atrophy 1 (OPA1), a GTPase at the inner mitochondrial membrane involved in regulating mitochondrial fusion, stability, and energy output, is known to be crucial for neural development: Opa1 heterozygous mice show abnormal brain development, and inactivating mutations in OPA1 are linked to human neurological disorders. Here, we used genetically modified human embryonic and patient-derived induced pluripotent stem cells and reveal that OPA1 haploinsufficiency leads to aberrant nuclear DNA methylation and significantly alters the transcriptional circuitry in neural progenitor cells (NPCs). For instance, expression of the forkhead box G1 transcription factor, which is needed for GABAergic neuronal development, is repressed in OPA1+/− NPCs. Supporting this finding, OPA1+/− NPCs cannot give rise to GABAergic interneurons, whereas formation of glutamatergic neurons is not affected. Taken together, our data reveal that OPA1 controls nuclear DNA methylation and expression of key transcription factors needed for proper neural cell specification.

• Cieslar-Pobuda, Artur; Ahrens, Theresa Dorothee; Caglayan, Safak; Behringer, Sidney; Hannibal, Luciana & Staerk, Judith (2020). DNMT3B deficiency alters mitochondrial biogenesis and α‐ketoglutarate levels in human embryonic stem cells. Stem Cells.  ISSN 1066-5099.  38(11), s 1409- 1422 . doi: 10.1002/stem.3256 Full text in Research Archive. Show summary

  Embryonic stem cell renewal and differentiation is regulated by metabolites that serve as cofactors for epigenetic enzymes. An increase of α‐ketoglutarate (α‐KG), a cofactor for histone and DNA demethylases, triggers multilineage differentiation in human embryonic stem cells (hESCs). To gain further insight into how the metabolic fluxes in pluripotent stem cells can be influenced by inactivating mutations in epigenetic enzymes, we generated hESCs deficient for de novo DNA methyltransferases (DNMTs) 3A and 3B. Our data reveal a bidirectional dependence between DNMT3B and α‐KG levels: a‐KG is significantly upregulated in cells deficient for DNMT3B, while DNMT3B expression is downregulated in hESCs treated with α‐KG. In addition, DNMT3B null hESCs exhibit a disturbed mitochondrial fission and fusion balance and a switch from glycolysis to oxidative phosphorylation. Taken together, our data reveal a novel link between DNMT3B and the metabolic flux of hESCs.

• Banerjee, Debarshi; Cieslar-Pobuda, Artur; Zhu, Geyunjian Harry; Wiechec, Emilia & Patra, Hirak K. (2019). Adding Nanotechnology to the Metastasis Treatment Arsenal. TIPS - Trends in Pharmacological Sciences.  ISSN 0165-6147.  40(6), s 403- 418 . doi: 10.1016/j.tips.2019.04.002
• Behringer, Sidney; Wingert, Victoria; Oria, Victor; Schumann, Anke; Grünert, Sarah; Cieslar-Pobuda, Artur; Kölker, Stefan; Lederer, Ann-Kathrin; Jacobsen, Donald W.; Stärk, Judith; Schilling, Oliver; Spiekerkoetter, Ute & Hannibal, Luciana (2019). Targeted metabolic profiling of methionine cycle metabolites and redox thiol pools in mammalian plasma, cells and urine. Metabolites.  ISSN 2218-1989.  9:235(10), s 1- 27 . doi: 10.3390/metabo9100235 Full text in Research Archive.
• Dung, Truong Manh; Ngoc, Vo Truong Nhu; Hiep, Nguyen Hung; Khoi, Truong Dinh; Xiem, Vu Van; Chu-Dinh, Thien; Cieslar-Pobuda, Artur; Stoufi, Eleana; Show, Pau Loke; Tao, Yang; Bac, Nguyen Duy; Van Ba, Nguyen; Le, Quynh-Anh; Pham, Van-Huy & Chu, Dinh Toi (2019). Evaluation of dental arch dimensions in 12 year-old Vietnamese children - A cross-sectional study of 4565 subjects. Scientific Reports.  ISSN 2045-2322.  9(1) . doi: 10.1038/s41598-019-39710-4 Full text in Research Archive.
• Skånland, Sigrid Strand & Cieslar-Pobuda, Artur (2019). Off-label uses of drugs for depression. European Journal of Pharmacology.  ISSN 0014-2999.  865 . doi: 10.1016/j.ejphar.2019.172732 Full text in Research Archive.
• Cieslar-Pobuda, Artur; Knoflach, Viktoria; Ringh, Mikael V.; Stark, Joachim; Likus, Wirginia; Siemianowicz, Krzysztof; Ghavami, Saeid; Hudecki, Andrzej; Green, Jason L. & Łos, Marek J. (2017). Transdifferentiation and reprogramming: Overview of the processes, their similarities and differences. BBA - Molecular Cell Research.  ISSN 0167-4889.  1864(7), s 1359- 1369 . doi: 10.1016/j.bbamcr.2017.04.017
• Skonieczna, Magdalena; Cieslar-Pobuda, Artur; Saenko, Yuriy; Foksinski, Marek; Olinski, Ryszard; Rzeszowska-Wolny, Joanna & Wiechec, Emilia (2017). The impact of dids-induced inhibition of Voltage-Dependent Anion Channels (VDAC) on cellular response of lymphoblastoid cells to ionizing radiation. Medicinal chemistry.  ISSN 1573-4064.  13(5), s 477- 483 . doi: 10.2174/1573406413666170421102353
• Skonieczna, Magdalena; Hejmo, Tomasz; Poterala-Hejmo, Aleksandra; Cieslar-Pobuda, Artur & Buldak, Rafal J. (2017). NADPH Oxidases (NOX): Insights into Selected Functions and Mechanisms of Action in Cancer and Stem Cells. Oxidative Medicine and Cellular Longevity.  ISSN 1942-0900.  2017:9420539, s 1- 15 . doi: 10.1155/2017/9420539
• Cieslar-Pobuda, Artur; Rafat, Mehrdad; Knoflach, Viktoria; Skonieczna, Magdalena; Hudecki, Andrzej; Malecki, Andrzej; Urasinska, Elzbieta; Ghavami, Seaid & Los, Marek J. (2016). Human induced pluripotent stem cell differentiation and direct transdifferentiation into corneal epithelial-like cells. OncoTarget.  ISSN 1949-2553.  7(27), s 42314- 42329 . doi: 10.18632/oncotarget.9791

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• Ahrens, Theresa Dorothee; Caglayan, Safak; Staerk, Judith & Cieslar-Pobuda, Artur (2018). Transdifferentiation – changing cell identity. Academic Press.  ISBN 9780128122785.  249 s.
• Caglayan, Safak; Ahrens, Theresa Dorothee; Cieslar-Pobuda, Artur & Staerk, Judith (2018). Modern ways of obtaining stem cells. Academic Press.  ISBN 9780128122785.  249 s.

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• Cieslar-Pobuda, Artur; Yue, Jianbo; Lee, Hsin-Chen; Skonieczna, Magdalena & Wei, Yau-Huei (2017). ROS and oxidative stress in stem cells. Oxidative Medicine and Cellular Longevity.  ISSN 1942-0900.  2017:5047168, s 1- 2 . doi: 10.1155/2017/5047168

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