Xian Hu

• Reint, Ganna; Li, Zhuokun; Labun, Kornel; Keskitalo, Salla; Soppa, Inkeri & Mamia, Katariina Aino Inkeri [Vis alle 19 forfattere av denne artikkelen] (2021). Rapid genome editing by CRISPR-Cas9-POLD3 fusion. eLIFE. ISSN 2050-084X. doi: 10.7554/eLife.75415.
• Skjeldal, Frode Miltzow; Haugen, Linda Hofstad; Nunes De C Mateus, Duarte; Frei, Dominik Michael; Rødseth, Anna Vik & Hu, Xian [Vis alle 7 forfattere av denne artikkelen] (2021). De novo formation of early endosomes during Rab5-to-Rab7a transition. Journal of Cell Science. ISSN 0021-9533. 134(8). doi: 10.1242/jcs.254185. Fulltekst i vitenarkiv Vis sammendrag

  Rab5 and Rab7a are the main determinants of early and late endosomes and are important regulators of endosomal progression. The transport from early endosomes to late endosome seems to be regulated through an endosomal maturation switch, where Rab5 is gradually exchanged by Rab7a on the same endosome. Here, we provide new insight into the mechanism of endosomal maturation, for which we have discovered a stepwise Rab5 detachment, sequentially regulated by Rab7a. The initial detachment of Rab5 is Rab7a independent and demonstrates a diffusion-like first-phase exchange between the cytosol and the endosomal membrane, and a second phase, in which Rab5 converges into specific domains that detach as a Rab5 indigenous endosome. Consequently, we show that early endosomal maturation regulated through the Rab5-to-Rab7a switch induces the formation of new fully functional Rab5-positive early endosomes. Progression through stepwise early endosomal maturation regulates the direction of transport and, concomitantly, the homeostasis of early endosomes.

• Wu, Hua; Chen, Yuyuan; Li, Bin; Li, Chun; Guo, Jun & You, Jia [Vis alle 12 forfattere av denne artikkelen] (2020). Targeting ROCK1/2 blocks cell division and induces mitotic catastrophe in hepatocellular carcinoma. Biochemical Pharmacology. ISSN 0006-2952. 184, s. 1–11. doi: 10.1016/j.bcp.2020.114353. Fulltekst i vitenarkiv
• Guadagno, Noemi Antonella; Margiotta, Azzurra; Bjørnestad, Synne Arstad; Haugen, Linda Hofstad; Kjos, Ingrid & Xiaochun, Xu [Vis alle 10 forfattere av denne artikkelen] (2020). Rab18 regulates focal adhesion dynamics by interacting with kinectin-1 at the endoplasmic reticulum . Journal of Cell Biology. ISSN 0021-9525. 219(7), s. 1–23. doi: 10.1083/JCB.201809020. Fulltekst i vitenarkiv
• Hu, Xian; Jalal, Salma; Sheetz, Michael; Bakke, Oddmund & Margadant, Felix Martin (2020). Micro-stepping extended focus reduces photobleaching and preserves structured illumination super-resolution features. Journal of Cell Science. ISSN 0021-9533. 133, s. 1–12. doi: 10.1242/jcs.240796.
• Le, Shimin; Hu, Xian; Yao, Mingxi; Chen, Hu; Yu, Miao & Xu, Xiaochun [Vis alle 10 forfattere av denne artikkelen] (2017). Mechanotransmission and Mechanosensing of Human alpha-Actinin 1. Cell reports. ISSN 2211-1247. 21(10), s. 2714–2723. doi: 10.1016/j.celrep.2017.11.040.
• Hu, Xian; Margadant, Felix Martin; Yao, Mingxi & Sheetz, Michael Patrick (2017). Molecular stretching modulates mechanosensing pathways. Protein Science. ISSN 0961-8368. 26(7), s. 1337–1351. doi: 10.1002/pro.3188.
• Hu, Xian; Jing, Chaoran; Xiaochun, Xu; Nakazawa, Naotaka; Cornish, Virginia W. & Margadant, Felix Martin [Vis alle 7 forfattere av denne artikkelen] (2016). Cooperative Vinculin Binding to Talin Mapped by Time-Resolved Super Resolution MIcorscopy. Nano Letters. ISSN 1530-6984. doi: 10.1021/acs.nanolett.6b00650.
• Mingxi, Yao; Goult, Benjamin T.; Klapholz, Benjamin; Hu, Xian; Toseland, Christopher P. & Yingjiang, Guo [Vis alle 9 forfattere av denne artikkelen] (2016). The Mechanical Response of Talin. Nature Communications. ISSN 2041-1723. doi: 10.1038/ncomms11966. Vis sammendrag

  Talin, a force-bearing cytoplasmic adapter essential for integrin-mediated cell adhesion, links the actin cytoskeleton to integrin-based cell–extracellular matrix adhesions at the plasma membrane. Its C-terminal rod domain, which contains 13 helical bundles, plays important roles in mechanosensing during cell adhesion and spreading. However, how the structural stability and transition kinetics of the 13 helical bundles of talin are utilized in the diverse talin-dependent mechanosensing processes remains poorly understood. Here we report the force-dependent unfolding and refolding kinetics of all talin rod domains. Using experimentally determined kinetics parameters, we determined the dynamics of force fluctuation during stretching of talin under physiologically relevant pulling speeds and experimentally measured extension fluctuation trajectories. Our results reveal that force-dependent stochastic unfolding and refolding of talin rod domains make talin a very effective force buffer that sets a physiological force range of only a few pNs in the talin-mediated force transmission pathway.

• Schvartzman, Mark; Palma, Matteo; Sable, Julia; Abramson, Justin; Hu, Xian & Sheetz, Michael Patrick [Vis alle 7 forfattere av denne artikkelen] (2011). Nanolithographic Control of the Spatial Organization of Cellular Adhesion Receptors at the Single- Molecule Level. Nano Letters. ISSN 1530-6984. doi: 10.1021/nl104378f.
• Zhang, Xin; Tee, YH; Heng, JK; Zhu, Y; Hu, Xian & Margadant, F [Vis alle 10 forfattere av denne artikkelen] (2010). Kinectin-mediated endoplasmic reticulum dynamics supports focal adhesion growth in the cellular lamella. Journal of Cell Science. ISSN 0021-9533. 123(22), s. 3901–3912. doi: 10.1242/jcs.069153.

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• Hu, Xian; Margadant, Felix Martin & Bakke, Oddmund (2021). International Patent: Microscope apparatus. WO2021160723A1. . Vis sammendrag

  A microscope apparatus (10) comprises: an imaging volume with orthogonal X, Y and Z axes; a camera (211) having a focal plane; a light source (110, 120) for illumination of the imaging volume; and a Z-stage movement system (131, 132) for relative movement of the imaging volume and the focal plane in the Z axis. Images of microscopic biological objects S are obtained using the microscope apparatus (10) via a method comprising: using the camera (211) to obtain an image in a single exposure whilst using the Z-stage movement system (131, 132) for relative movement of the imaging volume and the focal plane; wherein the Z axis movement is a step-wise movement in which there are multiple resting steps providing periods of no relative motion in between multiple periods of relative motion; and wherein during the periods of relative motion the light source is controlled to reduce the illumination of the imaging volume.

• Hu, Xian (2020). First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Xian Hu is first author on ‘Micro-stepping extended focus reduces photobleaching and preserves structured illumination super-resolution features’, published in JCS. Xian is a Postdoc in the lab of Oddmund Bakke at the Department of Biosciences University of Oslo, Norway, investigating the link betw. Journal of Cell Science. ISSN 0021-9533. doi: 10.1242/jcs.247320.
• Xiaochun, Xu; Hu, Xian; Sheetz, Michael Patrick & Margadant, Felix Martin (2017). Distortion Correction for Pillar Tracking Caused by Specimen and Devices.

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