Sandra Lopez Aviles

• Martîn, Ruth Martîn; Stonyte, Vilte & Lopez Aviles, Sandra (2020). Protein phosphatases in G1 regulation. International Journal of Molecular Sciences.  ISSN 1422-0067.  21:395(2), s 1- 15 . doi: 10.3390/ijms21020395 Vis sammendrag

  Eukaryotic cells make the decision to proliferate, to differentiate or to cease dividing during G1, before passage through the restriction point or Start. Keeping cyclin-dependent kinase (CDK) activity low during this period restricts commitment to a new cell cycle and is essential to provide the adequate timeframe for the sensing of environmental signals. Here, we review the role of protein phosphatases in the modulation of CDK activity and as the counteracting force for CDK-dependent substrate phosphorylation, in budding and fission yeast. Moreover, we discuss recent findings that place protein phosphatases in the interface between nutritional signalling pathways and the cell cycle machinery.

• Stonyte, Vilte; Martîn, Ruth Martîn; Segura-Pena, Dario; Sekulic, Nikolina & Lopez Aviles, Sandra (2020). Requirement of PP2A-B56 Par1 for the Stabilization of the CDK Inhibitor Rum1 and Activation of APC/C Ste9 during Pre-Start G1 in S. pombe. iScience.  ISSN 2589-0042.  23(5) . doi: 10.1016/j.isci.2020.101063 Vis sammendrag

  Exit from the cell cycle during the establishment of quiescence and upon cell differentiation requires the sustained inactivation of CDK complexes. Fission yeast cells deprived of nitrogen halt cell cycle progression in pre-Start G1, before becoming quiescent or undergoing sexual differentiation. The CDK inhibitor Rum1 and the APC/C activator Ste9 are fundamental for this arrest, but both are down-regulated by CDK complexes. Here, we show that PP2A-B56Par1 is instrumental for Rum1 stabilization and Ste9 activation. In the absence of PP2A-B56Par1, cells fail to accumulate Rum1, and this results in persistent CDK activity, Ste9 inactivation, retention of the mitotic cyclin Cdc13, and impaired withdrawal from the cell cycle during nitrogen starvation. Importantly, mutation of a putative B56 interacting motif in Rum1 recapitulates these defects. These results underscore the relevance of CDK-counteracting phosphatases in cell differentiation, establishment of the quiescent state, and escape from it in cancer cells.

• Wang, Shixiong; Singh, Sachin Kumar; Katika, MR; Lopez Aviles, Sandra & Hurtado, Antoni (2018). High Throughput Chemical Screening Reveals Multiple Regulatory Proteins on FOXA1 in Breast Cancer Cell Lines. International Journal of Molecular Sciences.  ISSN 1422-0067. . doi: 10.3390/ijms19124123
• Wang, Shixiong; Singh, Sachin Kumar; Madhumohan, Katika; Lopez-Aviles, Sandra & Rodriguez, Antoni Hurtado (2018). High Throughput Chemical Screening Reveals Multiple Regulatory Proteins on FOXA1 in Breast Cancer Cell Lines. International Journal of Molecular Sciences.  ISSN 1422-0067.  19(4123) . doi: 10.3390/ijms19124123 Fulltekst i vitenarkiv.
• Martîn, Ruth Martîn; Portantier, Marina; Chica-Balaguera, Nathalia; Nyquist-Andersen, Mari; Mata, J & Lopez-Aviles, Sandra (2017). A PP2A-B55-Mediated Crosstalk between TORC1 and TORC2 Regulates the Differentiation Response in Fission Yeast. Current Biology.  ISSN 0960-9822.  27(2), s 175- 188 . doi: 10.1016/j.cub.2016.11.037 Fulltekst i vitenarkiv.
• Martîn, Ruth Martîn & Lopez-Aviles, Sandra (2017). Express yourself: how PP2A-B55Pab1 helps TORC1 talk to TORC2. Current Genetics.  ISSN 0172-8083.  64(1), s 43- 51 . doi: 10.1007/s00294-017-0721-8 Fulltekst i vitenarkiv.
• Rothe, Christiane; Rødland, Gro Elise; Anda, Silje; Stonyte, Vilte; Boye, Erik; Lopez-Aviles, Sandra & Grallert, Beata (2017). A checkpoint-independent mechanism delays entry into mitosis after UV irradiation. Journal of Cell Science.  ISSN 0021-9533.  130(23), s 4028- 4037 . doi: 10.1242/jcs.204693

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