Human ESC Neuronal differentiation scRNA-seq
Here we present a searchable interactive web-tool utilising ShinyCell for the scRNA-seq from human ESCs Day 0, and neuronal differentiation time points Day 7, 13 and 20. This allows for exploration of gene expression in individual cells and annotated cell populations during the timeline of the differentiation.
Human ESC Neuronal differentiation scATAC-seq
We have performed scATAC-seq to explore chromatin accessibility landscapes in the cells in the beginning (ESC, Day 0) and at differentiation endpoint (Day 20). The data has been analyzed using ArchR and is openly available in a web tool based on our in house ShinyArchR.UiO. Take a tour into the epigenetic landscape of these cells and utilize different functionalities to learn more about gene regulation during differentiation.
Citing our work: The multiomics study Samara et al., "A multi-omics approach to visualize early neuronal differentiation from hESCs in 4D" is published in iScience. The original preprint is in bioRxiv.org. The details of hESC differentiation is described in Star Protocols. Code is available in EskelandLab Github. Processed datasets are deposited in NCBI GEO: GSE192858.
This data generated at the University of Oslo and is available in these tools under the permissive CC BY 4.0 license.