Henrik Sverre Huitfeldt

• De Angelis, Paula; Schjølberg, Aasa Rambæk; Hughes, Juliana Bentes; Huitfeldt, Henrik; Andersen, Solveig Norheim & Østvold, Anne Carine (2018). Nondysplastic ulcerative colitis has high levels of the homologous recombination repair protein NUCKS1 and low levels of the DNA damage marker gamma-H2AX. Inflammatory Bowel Diseases. ISSN 1078-0998. 24(3), p. 593–600. doi: 10.1093/ibd/izx071. Full text in Research Archive
• Brinchmann, Bendik Christian; Le Ferrec, Eric; Bisson, William H; Podechard, Normand; Huitfeldt, Henrik & Gallais, Isabelle [Show all 10 contributors for this article] (2018). Evidence of Selective Activation of Aryl Hydrocarbon Receptor Nongenomic Calcium Signaling by Pyrene. Biochemical Pharmacology. ISSN 0006-2952. 158, p. 1–12. doi: 10.1016/j.bcp.2018.09.023. Full text in Research Archive
• Shi, Jihua; Scholz, Hanne ; Huitfeldt, Henrik & Line, Pål Dag (2014). The effect of hepatic progenitor cells on experimental hepatocellular carcinoma in the regenerating liver. Scandinavian Journal of Gastroenterology. ISSN 0036-5521. 49(1), p. 99–108. doi: 10.3109/00365521.2013.854406.
• Liu, Shuzheng; Wierød, Lene; Skarpen, Ellen; Grøsvik, Helga; Duan, GuangCai & Huitfeldt, Henrik (2013). EGF Activates Autocrine TGF alpha to Induce Prolonged EGF Receptor Signaling and Hepatocyte Proliferation. Cellular Physiology and Biochemistry. ISSN 1015-8987. 32(3), p. 511–522. doi: 10.1159/000354454.
• Shi, Jihua; Liu, Shuzheng; Wierød, Lene; Scholz, Hanne Bjørnson; Anmarkrud, Jarl Andreas & Huitfeldt, Henrik [Show all 8 contributors for this article] (2013). RAF-targeted therapy for hepatocellular carcinoma in the regenerating liver. Journal of Surgical Oncology. ISSN 0022-4790. 107(4), p. 393–401. doi: 10.1002/jso.23224.
• Flinder, Liv Ingrid; Wierød, Lene; Rosseland, Carola; Huitfeldt, Henrik & Skarpen, Ellen (2013). FAK regulates Cdk2 in EGF-stimulated primary cultures of hepatocytes. Journal of Cellular Physiology. ISSN 0021-9541. 228(6), p. 1304–1313. doi: 10.1002/jcp.24287.
• Zhou, Yun; Holmseth, Silvia; Guo, C; Hassel, Bjørnar; Höfner, G & Huitfeldt, Henrik [Show all 7 contributors for this article] (2012). Deletion of the γ-aminobutyric acid transporter 2 (GAT2 and SLC6A13) gene in mice leads to changes in liver and brain taurine contents. Journal of Biological Chemistry. ISSN 0021-9258. 287(42), p. 35733–35746. doi: 10.1074/jbc.M112.368175.
• Shi, Jihua; Huitfeldt, Henrik; Suo, Zhenhe & Line, Pål Dag (2011). Growth of Hepatocellular Carcinoma in the Regenerating Liver. Liver transplantation. ISSN 1527-6465. 17(7), p. 866–874. doi: 10.1002/lt.22325.
• Flinder, Liv Ingrid; Timofeeva, Olga A.; Rosseland, Carola; Wierød, Lene; Huitfeldt, Henrik & Skarpen, Ellen (2011). EGF-Induced ERK-Activation Downstream of FAK Requires Rac1-NADPH Oxidase. Journal of Cellular Physiology. ISSN 0021-9541. 226(9), p. 2267–2278. doi: 10.1002/jcp.22563.
• Wierød, L; Rosseland, CM; Lindeman, Birgitte; Oksvold, Morten Pedersen; Grøsvik, H & Skarpen, E [Show all 7 contributors for this article] (2008). Activation of the p53-p21(Cip1) pathway is required for CDK2 activation and S-phase entry in primary rat hepatocytes. Oncogene. ISSN 0950-9232. 27, p. 2763–2771. doi: 10.1038/sj.onc.1210937.
• Oksvold, Morten Pedersen; Funderud, Ane; Kvissel, Anne Katrine; Skarpen, Ellen; Henanger, Heidi Hatledal & Huitfeldt, Henrik [Show all 8 contributors for this article] (2008). Epidermal growth factor receptor levels are reduced in mice with targeted disruption of the protein kinase A catalytic subunit. BMC Cell Biology. ISSN 1471-2121. 9. doi: 10.1186/1471-2121-9-16.
• Rosseland, Carola; Wierød, Lene; Flinder, Liv; Oksvold, Morten Pedersen; Skarpen, Ellen & Huitfeldt, Henrik (2008). Distinct functions of H-Ras and K-Ras in proliferation and survival of primary hepatocytes due to selective activation of ERK and Pl3K. Journal of Cellular Physiology. ISSN 0021-9541. 215, p. 818–826. doi: 10.1002/jcp.21367.
• Samuelsen, Jan T.; Schwarze, Per E; Huitfeldt, Henrik; Thrane, Vibeke; Låg, Marit & Refsnes, Magne [Show all 8 contributors for this article] (2007). Regulation of rat alveolar type 2 cell proliferation in vitro involves type II cAMP-dependent protein kinase. American Journal of Physiology - Lung cellular and Molecular Physiology. ISSN 1040-0605. 292(1), p. L232–L239. doi: 10.1152/ajplung.00049.2006.
• Wierød, L; Rosseland, CM; Lindeman, Birgitte; Oksvold, MP; Grøsvik, H & Skarpen, E [Show all 7 contributors for this article] (2007). CDK2 regulation through P13K and CDK4 is necessary for cell cycle progression of primary rat hepatocytes. Cell Proliferation. ISSN 0960-7722. 40(4), p. 475–487. doi: 10.1111/j.1365-2184.2007.00451.x.
• Grundt, Kirsten; Haga, Ingvild Vågslid; Huitfeldt, Henrik & Østvold, Anne Carine (2007). Identification and characterization of two putative nuclear localization signals (NLS) in the DNA-binding protein MUCKS. BBA - Molecular Cell Research. ISSN 0167-4889. 1773, p. 1398–1406. doi: 10.1016/j.bbamcr.2007.05.013.
• Skarpen, Ellen; Flinder, Liv; Rosseland, Carola; Ørstavik, Sigurd; Wierød, Lene & Oksvold, Morten Pedersen [Show all 8 contributors for this article] (2007). MEK1 and MEK2 regulate distinct functions by sorting ERK2 to different intracellular compartments. The FASEB Journal. ISSN 0892-6638.
• Granum, Stine; Gjerstad, Vibeke Sundvold; Dai, Kezheng; Kolltveit, Kristin Melkevik; Hildebrand, Kjersti & Huitfeldt, Henrik [Show all 8 contributors for this article] (2006). Structure function analysis of SH2D2A isoforms expressed in T cells reveals a crucial role for the proline rich region encoded by SH2D2A exon 7. BMC Immunology. ISSN 1471-2172. 7.
• Paasche, J.D.; Attramadal, T.; Kristiansen, Kurt; Oksvold, M.P.; Johansen, H.K. & Huitfeldt, H.S. [Show all 8 contributors for this article] (2005). Subtype-specific sorting of the ETA endothelin receptor by a novel endocytic recycling signal for G protein-coupled receptors. Molecular Pharmacology. ISSN 0026-895X. 67, p. 1581–1590.
• Rosseland, Carola; Wierød, Lene; Oksvold, M.P.; Werner, H; Østvold, Anne C. & Thoresen, G. Hege [Show all 9 contributors for this article] (2005). Cytoplasmic retention of peroxide-activated ERK provides survival in primary cultures of rat hepatocytes. Hepatology. ISSN 0270-9139. 42, p. 200–2007.
• Skarpen, Ellen; Oksvold, Morten Pedersen; Grøsvik, Helga; Widnes, Christian & Huitfeldt, Henrik (2005). Altered Regulation of EGF Receptor Signaling Following a Partial Hepatectomy. Journal of Cellular Physiology. ISSN 0021-9541. 202, p. 707–716.
• Oksvold, Morten Pedersen; Thien, CB; Widerberg, J; Chantry, A; Huitfeldt, Henrik & Langdon, WY (2004). UV-radiation-induced internalization of the epidermal growth factor receptor requires distinct serine and tyrosine residues in the cytoplasmic carboxy-terminal domain. Radiation Research. ISSN 0033-7587. 161(6), p. 685–691.
• Oksvold, Morten Pedersen; Huitfeldt, Henrik & Langdon, WY (2004). Identification of 14-3-3 zeta as an EGF receptor interacting protein. FEBS Letters. ISSN 0014-5793. 569, p. 207–210.
• Slagsvold, Hege H.; Rosseland, Carola; Jacobs, Chris; Khuong, Erica; Gaarder, Mona & Fallgren, Åsa Birgitta [Show all 8 contributors for this article] (2003). High molecular weight DNA fragments are processed by caspase sensitive or caspase independent pathways in cultures of cerebellar granule neurons. Brain Research. ISSN 0006-8993. 984, p. 111–121.
• Oksvold, Morten Pedersen; Thien, CBF; Widerberg, Jannicke; Chantry, A; Huitfeldt, Henrik & Langdon, WY (2003). Serine mutations that abrogate ligand-induced ubiquitination and internalization of the EGF receptor do not affect c-Cbl association with the receptor. Oncogene. ISSN 0950-9232. 22, p. 8509–8518.
• Grundt, Kirsten; Skjeldal, Lars; Anthonsen, Henrik W.; Skauge, Tormod; Huitfeldt, Henrik & Østvold, Anne Carine (2002). A putative DNA-binding domain in the NUCKS protein. Archives of Biochemistry and Biophysics. ISSN 0003-9861. 407(2), p. 168–175.
• Oksvold, Morten Pedersen; Huitfeldt, Henrik; Østvold, Anne Carine & Skarpen, Ellen (2002). UV induces tyrosine kinase-independent internalisation and endosome arrest of the EGF receptor. Journal of Cell Science. ISSN 0021-9533. 115(4), p. 793–803. Show summary

  We have compared the activation and trafficking of epidermal growth factor receptor (EGFR) induced by UV light and EGF. Tyrosine phosphorylation of EGFR was not detected in UV-exposed cells by immunoblotting of whole cell lysates or EGFR immunoprecipitates with antibodies specific for each of the five activated autophosphorylation sites of EGFR. In addition, EGFR of UV-irradiated cells did not demonstrate increased (32)P-incorporation. However, UV-exposed cells demonstrated a gel mobility shift of EGFR, which was not abolished by alkaline phosphatase treatment. UV-exposure did not induce dimerisation of EGFR. Furthermore, UV induced internalisation of EGFR without polyubiquitination or degradation. UV-exposed EGFR was transferred to early endosomes and arrested in transferrin-accessible endosomes close to the cell surface. Whereas inhibition of the EGFR tyrosine kinase effectively inhibited tyrosine phosphorylation and internalisation of EGF-activated EGFR, internalisation of UV-exposed EGFR was unaffected. UV induced neither relocalisation of Shc and Grb2 nor activation of Raf, but activation of MEK and MAPK was observed. Our work indicates that UV induces internalisation of EGFR independent of its phosphorylation or receptor tyrosine kinase activation, and altered EGFR trafficking compared with ligand-activated receptor. In addition, MAPK activation by UV does not appear to be mediated by EGFR activation.

• Oksvold, Morten Pedersen; Skarpen, Ellen; Widerberg, Jannicke & Huitfeldt, Henrik (2002). Fluorescent histochemical techniques for analysis of intracellular signaling. Journal of Histochemistry and Cytochemistry. ISSN 0022-1554. 50(3), p. 289–303. Show summary

  Intracellular signaling relies on the orchestrated cooperation of signaling proteins and modules, their intracellular localization, and membrane trafficking. Recently, a repertoire of fluorescence-based techniques, which significantly increases our potential for detailed studies of the involved mechanisms, has been introduced. Microscopic techniques with increased resolution have been combined with improved techniques for detection of signaling proteins. Transfections of fluorescently tagged proteins have allowed in vivo microscopy of their trafficking and interactions with other proteins and intracellular structures. We present an overview of general signaling principles and a description of techniques based on fluorescent microscopy suited for studies of signaling mechanisms.

• Grundt, Kirsten; Skjeldal, Lars; Anthonsen, Henrik W.; Skauge, Tormod; Huitfeldt, Henrik & Østvold, Anne Carine (2002). A putative DNA-binding domain in the NUCKS protein. Archives of Biochemistry and Biophysics. ISSN 0003-9861. 407, p. 168–175. Show summary

  We have studied the DNA binding properties of a NUCKS derived, synthetic peptide containing an extended GRP motif. This peptide binds to random sequence DNA, but did not bind preferentially to poly (dA-dT). A synthetic peptide with the same amino acid composition but with a random sequence did not bind to DNA, suggesting that the structure of the DNA binding domain plays a pivotal role for the interaction with DNA. NMR and graphic modeling were employed to investigate the structure of the synthetic peptide. It was shown that the DNA binding peptide constituted an a¿helix in phosphate buffer at pH 5.5. Docking results indicated an almost perfect fit for this small, helical peptide into the major groove of DNA with the possibility of four basic residues to interact with the phosphate backbone of DNA. One consensus site for phosphorylation by Cdk1 is located in the N-terminal end of the DNA binding peptide. Upon phosphorylation of this site, the binding to DNA was completely prohibited. Immunofluorescence experiments showed that NUCKS was located in the nuclei in proliferating cells in interphase of the cell cycle, but was distributed throughout the cytoplasm in mitotic cells.

• Farstad, Inger Nina; Malavasi, F.; Haraldsen, Guttorm; Huitfeldt, Henrik & Brandtzæg, Per (2002). CD38 is a marker of human lacteals. Virchows Archiv. ISSN 0945-6317. 441, p. 605–613.
• Oksvold, Morten Pedersen; Huitfeldt, Henrik; Stang, Espen & Madshus, Inger Helene (2002). Localizing the EGF receptor. Nature Cell Biology. ISSN 1465-7392. 4, p. E22–E23.
• Oksvold, Morten Pedersen; Skarpen, Ellen; Wierød, Lene; Paulsen, Ragnhild Elisabeth & Huitfeldt, Henrik (2001). Re-localization of activated EGF receptor and its signal transducers to multivesicular compartments downstream of early endosomes in response to EGF. European Journal of Cell Biology. ISSN 0171-9335. 80(4), p. 285–294.
• Oksvold, Morten Pedersen; Skarpen, Ellen & Huitfeldt, Henrik (2000). Intracellular signaling from the epidermal groawth factor receptor: Application of immunological methods. In Georgijev, P.; Olsnes, Sjur & Kozlov, Yuri (Ed.), Current problems of molecular genetics and cell biology. Center for Medical Studies, Moscow. p. 102–109.
• Oksvold, Morten Pedersen; Skarpen, Ellen; Lindeman, Birgitte; Roos, Norbert & Huitfeldt, Henrik (2000). Immunocytochemical localization of She and activated EGF receptor in early endosomes after EGF stimulation of HeLa cells. Journal of Histochemistry and Cytochemistry. ISSN 0022-1554. 48(1), p. 21–33.
• Låg, M.; Skarpen, Ellen; Rozendaal, B. van ; Haagsman, H.P.; Huitfeldt, Henrik & Thrane, E.V. [Show all 7 contributors for this article] (2000). Cell-specific expression in CCAAT/enhancer binding protein delta (C/EBP delta) in epithelial lung cells. Experimental Lung Research. ISSN 0190-2148. 26, p. 383–399.
• Lindeman, Birgitte; Skarpen, Ellen; Oksvold, Morten Pedersen & Huitfeldt, Henrik (2000). The carcinogen 2-acetylaminofluorene inhibits activation and nuclear accumulation of cyclin-dependent kinase 2 in growth-induced rat liver. Molecular Carcinogenesis. ISSN 0899-1987. 27, p. 190–199.
• Lindeman, Birgitte; Skarpen, Ellen; Thoresen, G. Hege; Christoffersen, Thoralf; Wierød, Lene & Madshus, Inger Helene [Show all 7 contributors for this article] (1999). Alteration of G1 cell-cycle protein expression and induction of p53 but not p21/waf1 by the DNA-modifying carcinogen 2-acetylaminofluorene in growth-stimulated hepatocytes in vitro. Molecular Carcinogenesis. ISSN 0899-1987. 24, p. 36–46.
• Huitfeldt, Henrik; Lindeman, Birgitte; Skarpen, Ellen; Oksvold, Morten Pedersen & Wierød, Lene (1999). Hvorfor er dioksin farlig? Tidsskrift for Den norske legeforening. ISSN 0029-2001. 119, p. 4323–4328.
• Skarpen, Ellen; Thoresen, G. Hege; Tasken, Kjetil; Samuelsen, Jon Tore; Jahnsen, Tore & Schwarze, P. E. [Show all 7 contributors for this article] (1998). Localization of cAMP-dependent signal transducers in early rat liver carcinogenesis. Histochemistry and Cell Biology. ISSN 0948-6143. 109(3), p. 203–209. Show summary

  Cyclic AMP (cAMP) is an important regulator of liver growth and differentiation. The main intracellular cAMP receptor, cAMP-dependent protein kinase (PKA), consists of two regulatory (R) and two catalytic (C) subunits. There are two classes, RI and RII, of the regulatory subunit, giving rise to type I (RI2C2) and type II (RII2C2) PKA. The RI/RII ratio generally decreases during organ development, and increases during carcinogenesis. Alterations in this ratio have been implicated as an important factor in experimental and clinical carcinogenesis. We have studied the expression of RIalpha, RIIalpha, Calpha, and an important substrate of PKA, the cAMP-response element binding protein, during rat liver carcinogenesis. Two-color immunofluorescence and confocal laser scan microscopy were used to characterize localization of the cAMP-dependent signal transducers in hepatocytes, bile ducts, oval cells, and preneoplastic lesions. We found that bile ducts and oval cells (putative liver stem cells) contained a higher RI/RII ratio than hepatocytes and preneoplastic lesions. Thus, an altered RI/RII ratio was not detected during early rat liver carcinogenesis, but may contribute to differentiation of putative liver stem cells to hepatocytes.

• Lindeman, Birgitte; Skarpen, Ellen & Huitfeldt, Henrik (1998). Stress protein expression in rat liver during tumour promotion: induction of heat-shock protein 27 in hepatocytes exposed to 2-acetylaminofluorene. Carcinogenesis. ISSN 0143-3334. 19, p. 1559–1563.
• Skarpen, Ellen; Johannessen, Lars Erik; Bjerk, K.; Fasteng, H.; Guren, Tormod Kyrre & Lindeman, Birgitte [Show all 11 contributors for this article] (1998). Endocytosed epidermal growth factor (EGF) receptors contribute to the EGF-mediated growth arrest in A431 cells by inducing a sustained increase in p21/CIP1. Experimental Cell Research. ISSN 0014-4827. 243, p. 161–172.
• Brandtzæg, Per; Halstensen, Trond Sundby; Huitfeldt, Henrik & Valnes, K (1997). Immunofluorescence and immunoenzyme histochemistry. In Johnstone, AP & Turner, MW (Ed.), Immunochemistry 2 ¿ A Practical Approach. Oxford University Press, Oxford. p. 71–130.
• Estil, Svein Arne; Olsen, Wenche Myhrvold; Huitfeldt, Henrik & Haaskjold, Erling (1997). UVB-induced formation of (6-4) photoproducts in the rat corneal epithelium. Acta Ophthalmologica Scandinavica. ISSN 1395-3907. 75, p. 120–123.
• Thrane, EV; Becher, Rune; Låg, Marit; Refsnes, Magne; Huitfeldt, Henrik & Schwarze, Per E (1997). Differential distribution and increased levels of ras proteins during lung development. Experimental Lung Research. ISSN 0190-2148. 23(1), p. 35–49.
• Levy, Finn Olav; Rasmussen, Anne Marie; Tasken, Kjetil; Skålhegg, Bjørn Steen; Huitfeldt, Henrik & Funderud, Steinar [Show all 8 contributors for this article] (1996). Cyclic AMP-dependent protein kinase in human B cells. Localization of the regulatory subunit RIalpha with the B cell antigen receptor during B cell activation. European Journal of Immunology. ISSN 0014-2980. 26, p. 1290–1296.
• Lehne, Gustav; de Angelis, Paula; Clausen, Ole Petter Fraas; Hall, Kirsten Sundby; Huitfeldt, Henrik & Rugstad, Hans Erik (1994). Pharmacokinetics and cytotoxicity of epirubicin (EPI) in drug resistant human hepatoma cells(HB8065). International Journal of Oncology. ISSN 1019-6439. 4, p. 1229–1235. Show summary

  Cellular accumulation and intracellular distribution of the anthracycline epirubicin (EPI) were studied by flow cytometry and confocal laser scan microscopy in resistant (HB8065/R) and sensitive (HB8065/S) human hepatoma cells. Using peroxidase immunohistochemistry HB8065/R cells were shown to express the multidrug efflux transporter P-glycoprotein (Pgp). Net drug accumulation was detectable in both cell types within seconds of treatment, and the intracellular drug level increased to a plateau after 15 min in HB8065/R cells and after 90 min to a 4.2 times higher level in HB8065/S cells. A 50% growth inhibition (GI50) was obtained in HB8065/R cells with 47 times as high EPI dose as in HB8065/S cells. Verapamil (VPL) increased the cellular accumulation of EPI and decreased the growth inhibition in HB8065/R cells. The cellular pharmacokinetics and cytotoxicity of EPI in HB8065/R cells reflect the increased levels of Pgp compared to HB8065/S cells.

• Skålhegg, Bjørn Steen; Tasken, Kjetil; Hansson, Vidar; Huitfeldt, Henrik; Jahnsen, Tore & Lea, Tor Erling (1994). Location of cAMP-dependent protein kinase type I with the TCR/CD3 complex. Science. ISSN 0036-8075. 263(5143), p. 84–87.

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• Bolann, Bjørn Johan; Huber, Sandra; Ruzzin, Jérôme; Brox, Jan; Huitfeldt, Henrik & Monsen, Anne Lise Bjørke (2017). Miljøgifter i kosten handler om mer enn fisk. Tidsskrift for Den norske legeforening. ISSN 0029-2001. 137(14-15), p. 973–974. doi: 10.4045/tidsskr.17.0521.
• Bolann, Bjørn Johan; Huber, Sandra; Ruzzin, Jérôme; Brox, Jan; Huitfeldt, Henrik & Monsen, Anne Lise Bjørke (2017). Er miljøgifter i norsk kosthold skadelig for barn? Tidsskrift for Den norske legeforening. ISSN 0029-2001. 137(4), p. 295–297. doi: 10.4045/tidsskr.16.0978.
• Shi, Jihua; Line, Pål Dag & Huitfeldt, Henrik (2010). Growth of Hepatocellular Carcinoma in the Regenerating Liver. Liver transplantation. ISSN 1527-6465. 16(6), p. S76–S76.
• Oksvold, Morten Pedersen; Skarpen, Ellen; Roos, Norbert & Huitfeldt, Henrik (1999). Immunocytochemical localization of ligand-activated phosphorylated EGFR in endosomes. Proceedings of the American Association of Cancer Research. ISSN 0197-016X. 40.
• Rosseland, Carola & Huitfeldt, Henrik (2007). Oxidative stress and growth factor regulation of proliferative and survival signals in primary hepatocytes. Unipub forlag. ISSN 978-82-8072-705-3.
• Wierød, Lene & Huitfeldt, Henrik (2007). Regulation of cell cycle progression and cellular survival in primary rat hepatocytes. Unipub forlag. ISSN 978-82-8072-704-6.

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