Johanne Tracey Jacobsen

• Nakandakari-Higa, Sandra; Walker, Sarah; Canesso, Maria C. C.; Heide, Verena van der; Chudnovskiy, Aleksey & Kim, Dong-Yoon [Show all 18 contributors for this article] (2024). Universal recording of immune cell interactions in vivo. Nature. ISSN 0028-0836. 627(8003), p. 399–406. doi: 10.1038/s41586-024-07134-4. Full text in Research Archive
• Graca, Luis; Jacobsen, Johanne Tracey & Kumar, Saumya (2023). The expanding family of T follicular regulatory cells. Science immunology. 8(82). doi: 10.1126/sciimmunol.adg7526. Full text in Research Archive
• Huszthy, Peter Csaba; Gopalakrishnan, Ramakrishna Prabhu; Jacobsen, Johanne Tracey; Haabeth, Ole Audun; Løset, Geir Åge & Braathen, Ranveig [Show all 10 contributors for this article] (2019). B cell receptor ligation induces display of V-region peptides on MHC class II molecules to T cells. Proceedings of the National Academy of Sciences of the United States of America (PNAS). ISSN 0027-8424. 116(51), p. 25850–25859. doi: 10.1073/pnas.1902836116. Full text in Research Archive Show summary

  The B cell receptors (BCRs) for antigen express variable (V) regions that are enormously diverse, thus serving as markers on individual B cells. V region-derived idiotypic (Id) peptides can be displayed as pId:MHCII complexes on B cells for recognition by CD4+ T cells. It is not known if naive B cells spontaneously display pId:MHCII in vivo or if BCR ligation is required for expression, thereby enabling collaboration between Id+ B cells and Id-specific T cells. Here, using a mouse model, we show that naive B cells do not express readily detectable levels of pId:MHCII. However, BCR ligation by Ag dramatically increases physical display of pId:MHCII, leading to activation of Id-specific CD4+ T cells, extrafollicular T-B cell collaboration and some germinal center formation, and production of Id+ IgG. Besides having implications for immune regulation, the results may explain how persistent activation of self-reactive B cells induces the development of autoimmune diseases and B cell lymphomas.

• Andersen, Tor Kristian; Huszthy, Peter Csaba; Gopalakrishnan, Ramakrishna Prabhu; Jacobsen, Johanne Tracey; Fauskanger, Marte & Tveita, Anders Aune [Show all 8 contributors for this article] (2019). Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules. npj Vaccines. 4:9, p. 1–13. doi: 10.1038/s41541-019-0101-0. Full text in Research Archive Show summary

  Enhancing the germinal center (GC) reaction is a prime objective in vaccine development. Targeting of antigen to MHCII on APCs has previously been shown to increase antibody responses, but the underlying mechanism has been unclear. We have here investigated the GC reaction after targeting antigen to MHCII in (i) a defined model with T and B cells of known specificity using adjuvant-free vaccine proteins, and (ii) an infectious disease model using a DNA vaccine. MHCII-targeting enhanced presentation of peptide: MHCII on APCs, and increased the numbers of GC B cells, TFH, and plasma cells. Antibodies appeared earlier and levels were increased. BCR of GC B cells and serum antibodies had increased avidity for antigen. The improved responses required cross-linking of BCR and MHCII in either cis or trans. The enhanced GC reaction induced by MHCII-targeting of antigen has clear implications for design of more efficient subunit vaccines.

• Jacobsen, Johanne Tracey; Mesin, Luka; Markoulaki, Styliani; Schiepers, Ariën; Cavazzoni, Cecilia B. & Bousbaine, Djenet [Show all 8 contributors for this article] (2018). One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation. Journal of Experimental Medicine (JEM). ISSN 0022-1007. 215(10), p. 2686–2695. doi: 10.1084/jem.20172064. Full text in Research Archive Show summary

  We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation.

• Elssen, Catharina H.M.J. Van; Rashidian, Mohammad; Vrbanac, Vladimir; Wucherpfennig, Kai W.; Habre, Zeina El & Sticht, Jana [Show all 14 contributors for this article] (2017). Noninvasive imaging of human immune responses in a human xenograft model of graft-versus-host disease. Journal of Nuclear Medicine. ISSN 0161-5505. 58(6), p. 1003–1008. doi: 10.2967/jnumed.116.186007. Full text in Research Archive
• Ersching, Jonatan; Efeyan, Alejo; Mesin, Luka; Jacobsen, Johanne Tracey; Pasqual, Giulia & Grabiner, Brian C [Show all 9 contributors for this article] (2017). Germinal Center Selection and Affinity Maturation Require Dynamic Regulation of mTORC1 Kinase. Immunity. ISSN 1074-7613. 46(6), p. 1045–1058.e6. doi: 10.1016/j.immuni.2017.06.005. Full text in Research Archive
• Rashidian, Mohammad; Keliher, Edmund J.; Bilate, Angelina M.; Duarte, Joao N.; Wojtkiewicz, Gregory R. & Jacobsen, Johanne Tracey [Show all 11 contributors for this article] (2015). Noninvasive imaging of immune responses. Proceedings of the National Academy of Sciences of the United States of America (PNAS). ISSN 0027-8424. 112(19), p. 6146–6151. doi: 10.1073/pnas.1502609112. Full text in Research Archive
• Jacobsen, Johanne Tracey; Haabeth, Ole Audun; Tveita, Anders; Schjetne, Karoline W; Munthe, Ludvig Andre & Bogen, Bjarne (2014). Naive idiotope-specific B and T cells collaborate efficiently in the absence of dendritic cells. Journal of Immunology. ISSN 0022-1767. 192(9), p. 4174–4183. doi: 10.4049/jimmunol.1302359. Full text in Research Archive
• Jacobsen, Johanne Tracey; Gjerstad, Vibeke Sundvold; Skjeldal, Frode Miltzow; Andersen, Jan Terje; Abrahamsen, Greger & Bakke, Oddmund [Show all 8 contributors for this article] (2013). B-cell tolerance to the B-cell receptor variable regions. European Journal of Immunology. ISSN 0014-2980. 43(10), p. 2577–2587. doi: 10.1002/eji.201243203. Full text in Research Archive
• Jacobsen, Johanne Tracey; Lunde, Elin; Gjerstad, Vibeke Sundvold; Munthe, Ludvig Andre & Bogen, Bjarne (2010). The cellular mechanism by which complementary Id(+) and anti-Id antibodies communicate: T cells integrated into idiotypic regulation. Immunology and Cell Biology. ISSN 0818-9641. 88(5), p. 515–522. doi: 10.1038/icb.2009.118. Full text in Research Archive

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• Jacobsen, Johanne Tracey; Schjetne, Karoline W; Munthe, Ludvig Andre & Bogen, Bjarne (2012). Naive anti-Id B cells and Id-specific CD4(+) T cells collaborate efficiently to non infectious antigens in the absence of dendritic cells. Journal of Immunology. ISSN 0022-1767. 188. Full text in Research Archive
• Jacobsen, Johanne Tracey; Schjetne, Karoline W; Munthe, Ludvig Andre & Bogen, Bjarne (2011). New Immunoglobulin Knock-in Mice for Idiotype (Id)-Driven T-B Collaboration. Scandinavian Journal of Immunology. ISSN 0300-9475. 73(4), p. 388–388. Full text in Research Archive

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