Kanutte Huse

• Chauhan, Sudhir Kumar; Dunn, Claire; Andresen, Nikolai Kragøe; Røssevold, Andreas Hagen; Skorstad, Gjertrud & Sike, Adam [Show all 11 contributors for this article] (2024). Peripheral immune cells in metastatic breast cancer patients display a systemic immunosuppressed signature consistent with chronic inflammation. NPJ breast cancer. ISSN 2374-4677. 10(1). doi: 10.1038/s41523-024-00638-2.
• Spasevska, Ivana; Sharma, Ankush; Steen, Chloe Beate; Josefsson, Sarah Elisabet; Blaker, Yngvild Nuvin & Kolstad, Arne [Show all 26 contributors for this article] (2023). Diversity of intratumoral regulatory T cells in B-cell non-Hodgkin lymphoma. Blood Advances. ISSN 2473-9529. 7(23), p. 7216–7230. doi: 10.1182/bloodadvances.2023010158. Full text in Research Archive
• Huse, Kanutte; Bai, Baoyan; Hilden, Vera Irene; Bollum, Lise Kristin; Våtsveen, Thea Kristin & Munthe, Ludvig Andre [Show all 10 contributors for this article] (2022). Mechanism of CD79A and CD79B support for IgM+ B cell fitness through B cell receptor surface expression. Journal of Immunology. ISSN 0022-1767. 209(10), p. 2042–2053. doi: 10.4049/jimmunol.2200144. Show summary

  he BCR consists of surface-bound Ig and a heterodimeric signaling unit comprised of CD79A and CD79B. Upon cognate Ag recognition, the receptor initiates important signals for B cell development and function. The receptor also conveys Ag-independent survival signals termed tonic signaling. Although the requirement of a CD79A/CD79B heterodimer for BCR complex assembly and surface expression is well established based on mice models, few studies have investigated this in human mature B cells. In this study, we found that human tonsillar B cells with high surface expression of IgM or IgG had potentiated BCR signaling compared with BCRlow cells, and high IgM expression in germinal center B cells was associated with reduced apoptosis. We explored the mechanism for IgM surface expression by CRISPR/Cas9-induced deletion of CD79A or CD79B in four B lymphoma cell lines. Deletion of either CD79 protein caused loss of surface IgM in all cell lines and reduced fitness in three. From two cell lines, we generated stable CD79A or CD79B knockout clones and demonstrated that loss of CD79A or CD79B caused a block in N-glycan maturation and accumulation of immature proteins, compatible with retention of BCR components in the endoplasmic reticulum. Rescue experiments with CD79B wild-type restored surface expression of CD79A and IgM with mature glycosylation, whereas a naturally occurring CD79B G137S mutant disrupting CD79A/CD79B heterodimerization did not. Our study highlights that CD79A and CD79B are required for surface IgM expression in human B cells and illuminates the importance of the IgM expression level for signaling and fitness.

• Rye, Inga Hansine; Huse, Kanutte; Josefsson, Sarah Elisabet; Kildal, Wanja; Danielsen, Håvard Emil Greger & Schlichting, Ellen [Show all 28 contributors for this article] (2021). Breast cancer metastasis: immune profiling of lymph nodes reveals exhaustion of effector T cells and immunosuppression. Molecular Oncology. ISSN 1574-7891. 16(1), p. 88–103. doi: 10.1002/1878-0261.13047. Full text in Research Archive
• Sonnet, Friederike; Namork, Ellen; Stylianou, Eva; Gaare-Olstad, Ingvild; Huse, Kanutte & Andorf, Sandra [Show all 9 contributors for this article] (2020). Reduced polyfunctional T cells and increased cellular activation markers in adult allergy patients reporting adverse reactions to food. BMC Immunology. ISSN 1471-2172. 21, p. 1–14. doi: 10.1186/s12865-020-00373-w. Full text in Research Archive
• Rein Trifonas, Idun D.; Notø, Heidi Ødegaard; Bostad, Monica; Huse, Kanutte & Stokke, Trond (2020). Cell Cycle Analysis and Relevance for Single‐Cell Gating in Mass Cytometry. Cytometry Part A. ISSN 1552-4922. 97(8), p. 832–844. doi: 10.1002/cyto.a.23960. Full text in Research Archive
• Köksal, Hakan; Dillard, Pierre; Josefsson, Sarah Elisabet; Maggadottir, Solrun Melkorka; Pollmann, Sylvie Mireille & Fåne, Anne [Show all 16 contributors for this article] (2019). Preclinical development of CD37CAR T-cell therapy for treatment of B-cell lymphoma. Blood Advances. ISSN 2473-9529. 3(8), p. 1230–1243. doi: 10.1182/bloodadvances.2018029678. Full text in Research Archive
• Josefsson, Sarah Elisabet; Beiske, Klaus; Blaker, Yngvild Nuvin; Førsund, Mette S; Holte, Harald & Østenstad, Bjørn [Show all 15 contributors for this article] (2019). TIGIT and PD-1 mark intratumoral T cells with reduced effector function in B-cell non-Hodgkin lymphoma. Cancer immunology research. ISSN 2326-6066. 7(3), p. 355–362. doi: 10.1158/2326-6066.CIR-18-0351. Full text in Research Archive
• Huse, Kanutte; Wogsland, Cara Ellen; Polikowsky, Hannah G.; Diggins, Kirsten E.; Smeland, Erlend B & Myklebust, June [Show all 7 contributors for this article] (2019). Human Germinal Center B Cells Differ from Naive and Memory B Cells in CD40 Expression and CD40L-Induced Signaling Response. Cytometry Part A. ISSN 1552-4922. 95(4), p. 442–449. doi: 10.1002/cyto.a.23737.
• Bollum, Lise Kristin; Huse, Kanutte; Oksvold, Morten Pedersen; Bai, Baoyan; Hilden, Vera Irene & Forfang, Lise [Show all 10 contributors for this article] (2017). BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-β receptor type I ALK5. PLOS ONE. ISSN 1932-6203. 12(5). doi: 10.1371/journal.pone.0177188. Full text in Research Archive
• Josefsson, Sarah Elisabet Göthberg; Huse, Kanutte; Kolstad, Arne; Beiske, Klaus; Pende, Daniela & Steen, Chloe Beate [Show all 13 contributors for this article] (2017). T cells expressing checkpoint receptor TIGIT are enriched in follicular lymphoma tumors and characterized by reversible suppression of T-cell receptor signaling. Clinical Cancer Research. ISSN 1078-0432. 24(4), p. 870–881. doi: 10.1158/1078-0432.CCR-17-2337. Full text in Research Archive
• Wogsland, Cara Ellen; Greenplate, Allison Rae; Kolstad, Arne; Myklebust, June; Irish, Jonathan Michael & Huse, Kanutte (2017). Mass cytometry of follicular lymphoma tumors reveals intrinsic heterogeneity in proteins including HLA-DR and a deficit in nonmalignant plasmablast and germinal center B-cell populations. Cytometry Part B: Clinical Cytometry. ISSN 1552-4949. 92(1), p. 79–87. doi: 10.1002/cyto.b.21498.
• Polikowsky, Hannah G.; Wogsland, Cara E.; Diggins, Kirsten E.; Huse, Kanutte & Irish, Jonathan M. (2015). Cutting edge: Redox signaling hypersensitivity distinguishes human germinal center B cells. Journal of Immunology. ISSN 0022-1767. 195(4), p. 1364–1367. doi: 10.4049/jimmunol.1500904.
• Brodtkorb, Marianne; Lingjærde, Ole Christian; Huse, Kanutte; Trøen, Gunhild; Hystad, Marit Elise & Hilden, Vera Irene [Show all 12 contributors for this article] (2014). Whole-genome integrative analysis reveals expression signatures predicting transformation in follicular lymphoma. Blood. ISSN 0006-4971. 123(7), p. 1051–1054. doi: 10.1182/blood-2013-07-512392.
• Bakkebø, Maren; Huse, Kanutte; Hilden, Vera Irene; Forfang, Lise; Myklebust, June & Smeland, Erlend B [Show all 7 contributors for this article] (2012). SARA is dispensable for functional TGF-beta signaling. FEBS Letters. ISSN 0014-5793. 586(19), p. 3367–3372. doi: 10.1016/j.febslet.2012.07.027.
• Huse, Kanutte; Bakkebø, Maren; Waelchli, Sebastien; Oksvold, Morten Pedersen; Hilden, Vera Irene & Forfang, Lise [Show all 11 contributors for this article] (2012). Role of Smad Proteins in Resistance to BMP-Induced Growth Inhibition in B-Cell Lymphoma. PLOS ONE. ISSN 1932-6203. 7(10). doi: 10.1371/journal.pone.0046117. Full text in Research Archive
• Huse, Kanutte; Bakkebø, Maren Kolltveit; Oksvold, Morten Pedersen; Forfang, Lise; Hilden, Vera Irene & Stokke, Trond [Show all 8 contributors for this article] (2011). Bone morphogenetic proteins inhibit CD40L/IL-21-induced Ig production in human B cells: Differential effects of BMP-6 and BMP-7. European Journal of Immunology. ISSN 0014-2980. 41(11), p. 3135–3145. doi: 10.1002/eji.201141558.
• Bakkebø, Maren Kolltveit; Huse, Kanutte; Hilden, Vera Irene; Smeland, Erlend B & Oksvold, Morten Pedersen (2010). TGF-beta-induced growth inhibition in B-cell lymphoma correlates with Smad1/5 signalling and constitutively active p38 MAPK. BMC Immunology. ISSN 1471-2172. 11. doi: 10.1186/1471-2172-11-57.
• Sivertsen, Einar; Huse, Kanutte; Hystad, Marit Elise; Kersten, Christian; Smeland, Erlend B & Myklebust, June (2007). Inhibitory effects and target genes of bone morphogenetic protein 6 in Jurkat Tag cells. European Journal of Immunology. ISSN 0014-2980. 37(10), p. 2937–2948.

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• Huse, Kanutte; Bakkebø, Maren; Forfang, Lise; Hilden, Vera Irene; Smeland, Erlend B & Myklebust, June (2010). Bone Morphogenetic Proteins Inhibit CD40L-and IL-21-Induced Ig Production In Human B Cells: Differential Effects of BMP-6 and BMP-7. Blood. ISSN 0006-4971. 116(21), p. 729–729.

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