David Kunke

• Shahrouzi, Parastoo; Azimzade, Youness; Brankiewicz-Kopcinska, Wioletta; Bhatia, Sugandha; Kunke, David & Richard, Derek [Show all 9 contributors for this article] (2024). Loss of chromosome cytoband 13q14.2 orchestrates breast cancer pathogenesis and drug response. Breast Cancer Research. ISSN 1465-5411. 26. doi: 10.1186/s13058-024-01924-4. Full text in Research Archive Show summary

  Breast cancer (BCa) is a major global health challenge. The BCa genome often carries extensive somatic copy number alterations (CNAs), including gains/amplifications and losses/deletions. These CNAs significantly affect tumor development, drug response and patient survival. However, how individual CNAs contribute is mostly elusive. We identified loss of chromosome 13q14.2 as a key CNA in BCa, occurring in up to 63% of patients, depending on the subtype, and correlating with poor survival. Through multi-omics and in vitro analyses, we uncover a paradoxical role of 13q14.2 loss, promoting both cell cycle and pro-apoptotic pathways in cancer cells, while also associating with increased NK cell and macrophage populations in the tumor microenvironment. Notably, 13q14.2 loss increases BCa susceptibility to BCL2 inhibitors, both in vitro and in patient-derived xenografts. Thus, 13q14.2 loss could serve as a biomarker for BCa prognosis and treatment, potentially improving outcomes for BCa patients. Graphical abstract

• Pisal, Rishikaysh V.; Suchánek, Jakub; Siller, Richard; Soukup, Tomáš; Hrebíková, Hana & Bezrouk, Ales [Show all 11 contributors for this article] (2018). Directed reprogramming of comprehensively characterized dental pulp stem cells extracted from natal tooth. Scientific Reports. 8(1). doi: 10.1038/s41598-018-24421-z. Full text in Research Archive Show summary

  The aim of this study was to extensively characterise natal dental pulp stem cells (nDPSC) and assess their efficiency to generate human induced pluripotent stem cells (hiPSC). A number of distinguishing features prompted us to choose nDPSC over normal adult DPSC, in that they differed in cell surface marker expression and initial doubling time. In addition, nDPSC expressed 17 out of 52 pluripotency genes we analysed, and the level of expression was comparable to human embryonic stem cells (hESC). Ours is the first group to report comprehensive characterization of nDPSC followed by directed reprogramming to a pluripotent stem cell state. nDPSC yielded hiPSC colonies upon transduction with Sendai virus expressing the pluripotency transcription factors POU5F1, SOX2, c-MYC and KLF4. nDPSC had higher reprogramming efficiency compared to human fibroblasts. nDPSC derived hiPSCs closely resembled hESC in terms of their morphology, expression of pluripotency markers and gene expression profiles. Furthermore, nDPSC derived hiPSCs differentiated into the three germ layers when cultured as embryoid bodies (EB) and by directed differentiation. Based on our findings, nDPSC present a unique marker expression profile compared with adult DPSC and possess higher reprogramming efficiency as compared with dermal fibroblasts thus proving to be more amenable for reprogramming.

• Rolseth, Veslemøy; Krokeide, Silje Z; Kunke, David; Neurauter, Christine Gran; Suganthan, Rajikala & Sejersted, Yngve [Show all 9 contributors for this article] (2013). Loss of Neil3, the major DNA glycosylase activity for removal of hydantoins in single stranded DNA, reduces cellular proliferation and sensitizes cells to genotoxic stress. BBA - Molecular Cell Research. ISSN 0167-4889. 1833(5), p. 1157–1164. doi: 10.1016/j.bbamcr.2012.12.024. Full text in Research Archive
• Hviid, Claus Vinter Bødker; Samulin-Erdem, Johanna Maria; Kunke, David; Ahmed, Muhammad Shakil; Kjeldsen, Signe Flood & Wang, Yun Yong [Show all 8 contributors for this article] (2012). The matri-cellular proteins 'cysteine-rich, angiogenic-inducer, 61' and 'connective tissue growth factor' are regulated in experimentally-induced sepsis with multiple organ dysfunction. Innate Immunity. ISSN 1753-4259. 18(5), p. 717–726. doi: 10.1177/1753425912436764. Full text in Research Archive
• Sejersted, Yngve; Hildrestrand, Gunn Annette; Kunke, David; Rolseth, Veslemøy; Krokeide, Silje Z. & Neurauter, Christine Gran [Show all 13 contributors for this article] (2011). Endonuclease VIII-like 3 (Neil3) DNA glycosylase promotes neurogenesis induced by hypoxia-ischemia. Proceedings of the National Academy of Sciences of the United States of America (PNAS). ISSN 0027-8424. 108(46), p. 18802–18807. doi: 10.1073/pnas.1106880108. Full text in Research Archive
• Wang, Wei; Esbensen, Ying; Kunke, David; Suganthan, Rajikala; Rachek, Lyudmila & Bjørås, Magnar [Show all 7 contributors for this article] (2011). Mitochondrial DNA Damage Level Determines Neural Stem Cell Differentiation Fate. Journal of Neuroscience. ISSN 0270-6474. 31(26), p. 9746–9751. doi: 10.1523/JNEUROSCI.0852-11.2011. Full text in Research Archive
• Kunke, David; Bryja, V; Mygland, Line; Arenas, E & Krauss, Stefan (2009). Inhibition of canonical Wnt signaling promotes gliogenesis in P0-NSCs. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 386(4), p. 628–633. doi: 10.1016/j.bbrc.2009.06.084. Full text in Research Archive
• Lorico, Aurelio; Bratbak, daniel; Meyer, Johann; Kunke, David; Krauss, Stefan & Plott, Walter [Show all 10 contributors for this article] (2005). Gamma-glutamylcysteine synthetase and L-buthionine-(S,R)-sulfoximine: a new selection strategy for gene-transduced neural and hematopoietic stem/progenitor cells. Human Gene Therapy. ISSN 1043-0342. 16, p. 711–724. Full text in Research Archive
• Rappa, Germana; Kunke, David; Holter, Jethro Lee; Diep, D.; Meyer, Julian & Baum, C. [Show all 9 contributors for this article] (2004). Efficient expansion and gene transduction of mouse neural stem/progenitor cells on recombinant fibronectin. Neuroscience. ISSN 0306-4522. 124(4), p. 823–830. doi: 10.1016/j.neuroscience.2003.11. Full text in Research Archive

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