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Metabolomics

Mass Spectrometry Lab at the Department of Nutrition

The Metabolomics Lab offers analysis of metabolites by mass spectrometry based technology. The laboratory performs advanced analysis for most research groups at the department of nutrition, other departments at university of Oslo and Oslo university hospital. In addition, the laboratory conducts analysis for external clients on a commercial basis.

Services

  • The laboratory can carry out analyzes on biological fluids, tissue biopsies as well as matrices from a variety of different vegetables. In addition to equipment and expertise, the laboratory has great flexibility that allows the development and establishment of new methods and analyzes in a short time for current and future needs.
  • Laboratory of Analytical Chemistry and Metabolism performs targeted and untargeted metabolomics analysis.
    • Target metabolite analysis; these approaches quantify few metabolites with a high degree of accuracy. These techniques involve labeling with a stable isotope (deuterium-labeled) for internal standards to quantification. 
    • Untargeted metabolomics; profiling of all metabolites, known and unknown, within a sample.

    Untargeted metabolomics

    Profiling of all metabolites

    Number of analytes Matrix Method Instrument
    Untargeted multiple LCMS-ESI Qexactive

    Prices

    Please contact us for prices

    Target metabolite analysis

    2-mercaptoethanesulfonate (Mesna)

    Number of analytes Matrix Method Instrument
    1 plasma LCMS-ESI Qtrap 5500

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 220,- 25.000,-
    External 440,- 25.000,-

    5-methyltetrahydrofolate (5mTHF), folic acid (FA), 5-formyltrahydrofolate (5fTHF)

    Number of analytes Matrix Method Instrument
    3 plasma LCMS-ESI Qtrap 5500

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 300,- 25.000,-
    External 600,- 25.000,-

    8-iso-PGF 2α

    Number of analytes Matrix Method Instrument
    5-7 plasma/urin LCMS-ESI Qtrap 4000

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 320,- 25.000,-
    External 640,- 25.000,-

    25- and 27 hydroxycholesterol

    Number of analytes Matrix Method Instrument
    2 plasma LCMS-ESI Qtrap 4000

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 270,- 20.000,-
    External 540,- 20.000,-

    25- and 27 hydroxycholesterol

    Number of analytes Matrix Method Instrument
    2 plasma LCMS-ESI Qtrap 5500

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 270,- 25.000,-
    External 540,- 25.000,-

    Amino Acids

    Number of analytes Matrix Method Instrument
    23-28 plasma/vev/cell LCMS-ESI Qtrap 5500

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 400,- 25.000,-
    External 800,- 25.000,-

    Carotenoids

    Number of analytes Matrix Method Instrument
    5 plasma/serum HPLC-UV Agilent 1290

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 220,- 20.000,-
    External 440,- 20.000,-

    Cortisol, Cortisone and Tryptophan

    Number of analytes Matrix Method Instrument
    3 plasma LCMS-ESI Qtrap 5500

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 250,- 25.000,-
    External 500,- 25.000,-

    Folic acid

    Number of analytes Matrix Method Instrument
    1 plasma HPLC-UV Agilent 1290

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 230,- 20.000,-
    External 460,- 20.000,-

    Lactate & pyruvate

    Number of analytes Matrix Method Instrument
    2 plasma LCMS-ESI Qtrap 5500

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 270,- 25.000,-
    External 540,- 25.000,-

    Methylmalonic acid (MMA) & Succinic acid (SA)

    Number of analytes Matrix Method Instrument
    2 plasma LCMS-ESI Qtrap 5500

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 270,- 25.000,-
    External 540,- 25.000,-

    Plasma Bile Acids

    Number of analytes Matrix Method Instrument
    9 plasma LCMS-ESI Qtrap 5500

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 300,- 25.000,-
    External 600,- 25.000,-

    Plasma Nonestrified Free Fatty Acid (NEFA)

    Number of analytes Matrix Method Instrument
    13 plasma LCMS-ESI Qtrap 5500

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 400,- 25.000,-
    External 800,- 25.000,-

    Retinoids (Vitamin A)

    Number of analytes Matrix Method Instrument
    5-7 plasma/vev LCMS-APCI Qtrap 4000

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 350,- 25.000,-
    External 700,- 25.000,-

    S-Adenosylmethonine(SAM) & S-Adenosylhomocysteine (SAH)

    Number of analytes Matrix Method Instrument
    2 plasma LCMS-ESI Qtrap 5500

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 270,- 25.000,-
    External 540,- 25.000,-

    Tocopherols (Vitamin E)

    Number of analytes Matrix Method Instrument
    4 plasma HPLC-FLD Agilent 1290

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 250,- 20.000,-
    External 500,- 20.000,-

    Total fraction of Fatty Acids

    Number of analytes Matrix Method Instrument
    13 plasma GCMS AS 3000 Thermo

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 400,- 25.000,-
    External 600,- 25.000,-

    Vitamin B6

    Number of analytes Matrix Method Instrument
    1 plasma LCMS-ESI Qtrap 5500

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 220,- 25.000,-
    External 440,- 25.000,-

    Vitamin C

    Number of analytes Matrix Method Instrument
    2 plasma HPLC-UV Agilent 1290

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 230,- 20.000,-
    External 460,- 20.000,-

    Vitamin D

    Number of analytes Matrix Method Instrument
    1 plasma LCMS-ESI Qtrap 4000

    Prices

      Price/sample (NOK) Starting price (NOK)
    UiO users 270,- 25.000,-
    External 540,- 25.000,-

    Instrumentation

    • Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer
    • Applied BioSystems 4000 QTRAP triple-quadrupole
    • Applied BioSystems 5500 QTRAP triple-quadrupole
    • Focus GC AS3000 Autosampler
    • Agilent 1290 UPLC

    Prices

    Published Jan. 8, 2020 2:07 PM - Last modified Feb. 4, 2020 10:28 AM