Services
Our core facility conducts analyses on biological fluids and tissue biopsies using state-of-the-art instruments equipped with advanced software that is user-friendly, robust, and reproducible. These instruments also have applications for identifying new and unique small-molecule compounds.
In addition to our equipment and expertise, our core facility offers significant flexibility, allowing for the rapid development and establishment of new methods and analyses to meet both current and future needs.
Metabolomics:
We provide metabolite analysis using mass spectrometry-based technology. Our core facility performs advanced analyses for various research groups within the Department of Nutrition, other departments at the University of Oslo, and Oslo University Hospital.
Our core facility specializes in targeted metabolomics analyses. Targeted metabolite analysis quantifies a small number of metabolites with a high degree of accuracy. These techniques utilize stable isotopes (deuterium-labeled) as internal standards for quantification.
Biocrates MxP® Quant 500 Kit – Over 600 Biomarkers Available
The Metabolomics Core Facility offers analysis using the Biocrates MxP® Quant 500 Kit, enabling quantification of over 600 metabolites from various biological sample types. This standardized and high-throughput platform covers a broad range of metabolite classes, including amino acids, acylcarnitines, biogenic amines, phospholipids, ceramides, and many more.
https://biocrates.com/mxp-quant-500-kit/
For pricing and availability, please contact us directly.
Seahorse XFe24 Analyzer: Advanced platform for measuring cellular bioenergetics, mitochondrial function, and real-time metabolic fluxes (glycolysis and oxygen consumption rates) in living cells using a 24-well format.
Instruments:
- Q Exactive ™ Hybrid Quadrupole-Orbitrap ™ massespektrometer.
- Applied BioSystems 6500+ QTRAP triple-quadrupole
- GCMS Agilent intuvo 9000
- Agilent 1290 UPLC
- Seahorse XFe24
Prices
2-mercaptoethanesulfonate (Mesna)
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 1 | plasma | LCMS-ESI | Qtrap 6500 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 500,- | 25.000,- |
| External | 900,- | 25.000,- |
5-methyltetrahydrofolate (5mTHF), folic acid (FA), 5-formyltrahydrofolate (5fTHF)
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 3 | plasma | LCMS-ESI | Qtrap 6500 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 650,- | 25.000,- |
| External | 1100,- | 25.000,- |
8-iso-PGF 2α
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 5-7 | plasma/urin | LCMS-ESI | Qtrap 6500 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 800,- | 25.000,- |
| External | 1100,- | 25.000,- |
25- and 27 hydroxycholesterol
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 2 | plasma | LCMS-ESI | Qtrap 6500 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 600,- | 25.000,- |
| External | 1000,- | 25.000,- |
Amino Acids
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 23-28 | plasma/vev/cell | LCMS-ESI | Qtrap 6500 |
1-Methylhistidine 3-Methylhistidine Alanine AMBA Arginine Asparagine Aspartic Acid Citrulline Creatinine Cystathionine | Cysteine Glutamic Acid Glutamine Glutathione Glycine Histidine Homocysteine Isoleucine Leucine ketoleucine (α-Ketoisocaproic Acid ) | Lysine Methionine Ornithine Phenylalanine Proline Serine Taurine Threonine Tryptophan N-acetyltryptophan Tyrosine Valine |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 800,- | 25.000,- |
| External | 1200,- | 25.000,- |
Carotenoids
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 5 | plasma/serum | HPLC-UV | Agilent 1290 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 350,- | 25.000,- |
| External | 700,- | 25.000,- |
Cortisol, Cortisone and Tryptophan
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 3 | plasma | LCMS-ESI | Qtrap 6500 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 500,- | 25.000,- |
| External | 1000,- | 25.000,- |
Folic acid
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 1 | plasma | HPLC-UV | Agilent 1290 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 600,- | 20.000,- |
| External | 1000,- | 20.000,- |
Lactate & pyruvate
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 2 | plasma | LCMS-ESI | Qtrap 6500 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 600,- | 25.000,- |
| External | 1000,- | 25.000,- |
Methylmalonic acid (MMA) & Succinic acid (SA)
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 2 | plasma | LCMS-ESI | Qtrap 6500 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 600,- | 25.000,- |
| External | 1000,- | 25.000,- |
Plasma Bile Acids
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 9 | plasma | LCMS-ESI | Qtrap 6500 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 700,- | 25.000,- |
| External | 1200,- | 25.000,- |
Plasma Nonestrified Free Fatty Acid (NEFA)
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 13 | plasma | LCMS-ESI | Qtrap 6500 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 700,- | 25.000,- |
| External | 1200,- | 25.000,- |
Retinoids (Vitamin A)
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 5-7 | plasma/vev | LCMS-APCI | Qtrap 6500 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 700,- | 25.000,- |
| External | 1100,- | 25.000,- |
S-Adenosylmethonine(SAM) & S-Adenosylhomocysteine (SAH)
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 2 | plasma | LCMS-ESI | Qtrap 6500 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 600,- | 25.000,- |
| External | 1000,- | 25.000,- |
Tocopherols (Vitamin E)
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 4 | plasma | HPLC-FLD | Agilent 1290 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 500,- | 20.000,- |
| External | 1000,- | 20.000,- |
Total fraction of Fatty Acids
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 13 | plasma | GCMS | Agilent intuvo 9000 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 800,- | 25.000,- |
| External | 1200,- | 25.000,- |
Vitamin B6
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 1 | plasma | LCMS-ESI | Qtrap 6500 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 500,- | 25.000,- |
| External | 1000,- | 25.000,- |
Vitamin C
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 2 | plasma | HPLC-UV | Agilent 1290 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 500,- | 25.000,- |
| External | 1000,- | 25.000,- |
Vitamin D
| Number of analytes | Matrix | Method | Instrument |
|---|---|---|---|
| 1 | plasma | LCMS-ESI | Qtrap 6500 |
Prices
| Price/sample (NOK) | Starting price (NOK) | |
|---|---|---|
| UiO users | 600,- | 25.000,- |
| External | 1000,- | 25.000,- |
Instrumentation
- Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer
- Applied BioSystems 6500+ QTRAP triple-quadrupole
- GCMS Agilent intuvo 9000
- Agilent 1290 UPLC