Our toolbox
We combine targeted genetic perturbation, advanced imaging, and quantitative analysis to measure trafficking dynamics and causality in real time.
Cell models
Mammalian cell culture models for macropinocytosis and endocytic trafficking
Disease-relevant systems used to study cancer-associated rewiring of uptake and trafficking
Genetic perturbation and functional genetics
Genome editing for endogenous tagging and mechanistic perturbation
CRISPR-based screening approaches to identify regulators of uptake, maturation, and identity transitions
Pathogen-uptake models
Virus-like particle (VLP) uptake systems to model entry mechanisms
Imaging-based readouts to connect uptake phenotypes to molecular control points
Advanced microscopy
High-resolution live-cell imaging of membrane dynamics, ruffles, macropinosomes, and endosomal compartments
Light-sheet microscopy for fast 3D imaging with reduced phototoxicity
Quantitative imaging strategies aligned to vesicle identity transitions
Quantitative image analysis
Automated image processing pipelines for vesicle detection, tracking, and maturation timing
Quantification of identity marker dynamics during plasma membrane → endosome transitions