Giovanna Perinetti Casoni

• Casoni, Giovanna Perinetti & Malmberg, Karl-Johan (2026). Induced Pluripotent Stem Cell–Derived Natural Killer Cells: From Concept to Clinic. In Zimmer, Jacques & Ullrich, Evelyn (Ed.), Natural Killer Cells. Springer Nature link. ISSN 9783662688168. p. 37–37. doi: 10.1007/978-3-662-68816-8_66-1. Full text in Research Archive
• Kalinichenko, Artem; Huemer, Jakob; Humer, Theresa; Haimel, Matthias; Svaton, Michael & Socquet-Juglard, Nicolas [Show all 31 contributors for this article] (2025). Protein palmitoylation and sphingolipid metabolism control regulated exocytosis in cytotoxic lymphocytes. Science immunology. 10(112). doi: 10.1126/sciimmunol.ado3825. Full text in Research Archive Show summary

  Regulated exocytosis controls key cellular functions ranging from neurotransmitter release to the secretion of immune mediators, and its disruption is associated with numerous pathologies. The cytotoxic activity of lymphocytes is particularly dependent on regulated and polarized lytic granule delivery toward infected or malignant cells. Although genetic and mechanistic studies have identified factors regulating exocytosis in cytotoxic lymphocytes, a systematic mapping of the relevant factors and their relationships is lacking. Through a genome-scale CRISPR knockout screen in a human natural killer cell line, we characterized a complex genetic network regulating cytotoxic granule exocytosis, with lipid metabolism and protein lipidation among the most prominent pathways. By combining global protein palmitoylation and lipidomic studies, we found that ZDHHC17 drives palmitoylation of the core SNARE complex protein SNAP23 to target cytotoxic granules to GM1-rich lipid rafts whose assembly is controlled by serine palmitoyltransferase. In summary, our study identifies previously unrecognized factors essential for cytotoxic function in human lymphocytes and uncovers how lipid metabolism and protein palmitoylation are involved in the process of regulated exocytosis.

• Sekine, Takuya; Galgano, Donatella; Casoni, Giovanna Perinetti; Meeths, Marie; Cron, Randy Q. & Bryceson, Yenan (2024). CD8⁺ T Cell Biology in Cytokine Storm Syndromes. Advances in Experimental Medicine and Biology. ISSN 0065-2598. 1448, p. 129–144. doi: 10.1007/978-3-031-59815-9_10. Full text in Research Archive
• Gopalakrishnan, Nimi; Hannam, Ryan; Casoni, Giovanna Perenetti; Barriet, David; Ribe, Jonas Myren & Haug, Markus [Show all 7 contributors for this article] (2015). Infection and immunity on a chip: A compartmentalised microfluidic platform to monitor immune cell behaviour in real time. Lab on a Chip. ISSN 1473-0197. 15(6), p. 1481–1487. doi: 10.1039/c4lc01438c. Full text in Research Archive

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• Casoni, Giovanna Perinetti (2025). Improving functional gaps in iPSC-derived NK cells through transcriptional reference mapping. Full text in Research Archive
• Casoni, Giovanna Perinetti (2025). Correcting functional gaps in iPSC-derived NK cells through transcriptional reference mapping and CRISPR screens. Full text in Research Archive

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