Henry William Hoyle

• Frank, Anna Katharina; Chung, Brian K.; Novales, Miguel Larraz Lopez De; Engesæter, Lise Katrine; Hoyle, Henry William & Øgaard, Jonas Daniel Sjøberg [Show all 15 contributors for this article] (2024). Single-Cell Transcriptomic Profiling of Cholangiocyte Organoids Derived from Bile Ducts of Primary Sclerosing Cholangitis Patients. Digestive Diseases and Sciences. ISSN 0163-2116. 69, p. 3810–3823. doi: 10.1007/s10620-024-08570-y. Full text in Research Archive Show summary

  Background and Aims Primary sclerosing cholangitis (PSC) is a chronic inflammatory liver disorder without effective medical treatment which is characterized by inflammation and fibrotic structures around the bile ducts. Biliary epithelial cells (cholangiocytes) are the target and potential disease drivers in PSC, yet little is known if cholangiocytes from PSC patients differ from non-PSC controls. To characterize cholangiocytes at early rather than end-stage disease, cholangiocyte organoids (COs) were derived from diseased bile ducts of PSC patients and compared to organoids generated from disease controls. Methods Cholangiocytes were obtained during endoscopic retrograde cholangiopancreatography (ERCP) brushing of diseased bile duct areas and expanded as organoids using previously established culture methods. Stable CO lines were analyzed for cell type identity, basic cholangiocyte function, and transcriptomic signature. Results We demonstrate that cholangiocytes, derived from the damaged area within the bile ducts of PSC patients, can be expanded in culture without displaying functional or genetic disease-related features. We further show that COs from patients who later were diagnosed with dysplasia exhibit higher expression of the cancer-associated genes PGC, FXYD2, MIR4435-2HG, and HES1. Conclusions Our results demonstrate that PSC organoids are largely similar to control organoids after culture and highlight the significance of COs as a tool for regenerative medicine approaches as well as their potential for discovering new potential biomarkers for diagnosing cholangiocarcinoma.

• Hoyle, Henry William; Smith, Lucy; Williams, Richard & Przyborski, Stefan (2020). Applications of novel bioreactor technology to enhance the viability and function of cultured cells and tissues. Interface Focus. ISSN 2042-8898. 10. doi: 10.1098/rsfs.2019.0090. Full text in Research Archive

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