Anyone interested in meeting with Dr Schmierer can contact Monika Szymanska to arrange an appointment.
Title and Abstract:
High-Throughput CRISPR Perturbation as a Tool in Basic Research and Drug
Discovery
Genome-editing by CRISPR-Cas9 has empowered research by the development of ingenious explorative tools. The SciLifeLab High Throughput Genome Engineering facility (HTGE) is a research facility funded by the Swedish government to make these challenging technologies easily accessible to the research community. My talk will focus on two distinct highthroughput application areas of CRISPR-Cas9: 1) The identification of genes and pathways affecting phenotypes of interest by pooled lentiviral genetic screens using CRISPR-KO, CRISPR-inhibition and CRISPR-activation; and 2) the characterization of drug-protein or proteinprotein interactions by targeted mutagenesis using Cas9-fused base-editors.
Further information
Karolinska Institute High Throughput Genome Engineering webpage