NCMM seminar series: Dr Bernhard Schmierer, Karolinska Institute
NCMM presents a seminar by Dr Bernhard Schmierer, Senior Researcher and Head of the Genome Engineering Core Facility, SciLifelab and Karolinska Institute, Stockholm, Sweden.
Anyone interested in meeting with Dr Schmierer can contact Monika Szymanska to arrange an appointment.
Title and Abstract:
High-Throughput CRISPR Perturbation as a Tool in Basic Research and Drug
Genome-editing by CRISPR-Cas9 has empowered research by the development of ingenious explorative tools. The SciLifeLab High Throughput Genome Engineering facility (HTGE) is a research facility funded by the Swedish government to make these challenging technologies easily accessible to the research community. My talk will focus on two distinct highthroughput application areas of CRISPR-Cas9: 1) The identification of genes and pathways affecting phenotypes of interest by pooled lentiviral genetic screens using CRISPR-KO, CRISPR-inhibition and CRISPR-activation; and 2) the characterization of drug-protein or proteinprotein interactions by targeted mutagenesis using Cas9-fused base-editors.