Kirsten Grundt

• Østvold, Anne Carine; Grundt, Kirsten & Wiese, Claudia (2022). NUCKS1 is a highly modified, chromatin-associated protein involved in a diverse set of biological and pathophysiological processes. Biochemical Journal. ISSN 0264-6021. 479(11), s. 1205–1220. doi: 10.1042/BCJ20220075. Fulltekst i vitenarkiv
• Grundt, Kirsten; Thiede, Bernd & Østvold, Anne Carine (2017). Identification of kinases phosphorylating 13 sites in the nuclear, DNA-binding protein NUCKS. Biochimica et Biophysica Acta - Proteins and Proteomics. ISSN 1570-9639. 1865(3), s. 359–369. doi: 10.1016/j.bbapap.2016.12.009. Fulltekst i vitenarkiv
• Yue, Yangbo; Leung, Stanley G.; Liu, Yueyong; Huang, Yurong; Grundt, Kirsten & Østvold, Anne Carine [Vis alle 10 forfattere av denne artikkelen] (2016). Nucks1 synergizes with Trp53 to promote radiation lymphomagenesis in mice. OncoTarget. 7(38), s. 61874–61889. doi: 10.18632/oncotarget.11297. Fulltekst i vitenarkiv
• Parplys, Ann C.; Zhao, Weixing; Sharma, Neelam; Groesser, Torsten; Liang, Fengshan & Maranon, David G. [Vis alle 14 forfattere av denne artikkelen] (2015). NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic Acids Research (NAR). ISSN 0305-1048. 43(20), s. 9817–9834. doi: 10.1093/nar/gkv859. Fulltekst i vitenarkiv
• Drosos, Y; Kouloukoussa, M; Østvold, Anne Carine; Grundt, Kirsten; Goutas, N & Vlachodimitropoulos, D [Vis alle 11 forfattere av denne artikkelen] (2009). NUCKS overexpression in breast cancer. Cancer Cell International. 9. doi: 10.1186/1475-2867-9-19. Fulltekst i vitenarkiv Vis sammendrag

  Background NUCKS (Nuclear, Casein Kinase and Cyclin-dependent Kinase Substrate) is a nuclear, DNA-binding and highly phosphorylated protein. A number of reports show that NUCKS is highly expressed on the level of mRNA in several human cancers, including breast cancer. In this work, NUCKS expression on both RNA and protein levels was studied in breast tissue biopsies consisted of invasive carcinomas, intraductal proliferative lesions, benign epithelial proliferations and fibroadenomas, as well as in primary cultures derived from the above biopsies. Specifically, in order to evaluate the level of NUCKS protein in correlation with the histopathological features of breast disease, immunohistochemistry was employed on paraffin sections of breast biopsies of the above types. In addition, NUCKS expression was studied by means of Reverse Transcription PCR (RT-PCR), real-time PCR (qRT-PCR) and Western immunoblot analyses in the primary cell cultures developed from the same biopsies. Results The immunohistochemical Results showed intense NUCKS staining mostly in grade I and II breast carcinomas compared to normal tissues. Furthermore, NUCKS was moderate expressed in benign epithelial proliferations, such as adenosis and sclerosing adenosis, and highly expressed in intraductal lesions, specifically in ductal carcinomas in situ (DCIS). It is worth noting that all the fibroadenoma tissues examined were negative for NUCKS staining. RT-PCR and qRT-PCR showed an increase of NUCKS expression in cells derived from primary cultures of proliferative lesions and cancerous tissues compared to the ones derived from normal breast tissues and fibroadenomas. This increase was also confirmed by Western immunoblot analysis. Although NUCKS is a cell cycle related protein, its expression does not correlate with Ki67 expression, neither in tissue sections nor in primary cell cultures. Conclusion The results show overexpression of the NUCKS protein in a number of non malignant breast lesions and cancerous tissues. In particular, the NUCKS overexpression in ADH and DCIS indicates a significant role of this protein in neoplastic progression.

• Grundt, Kirsten; Haga, Ingvild Vågslid; Huitfeldt, Henrik & Østvold, Anne Carine (2007). Identification and characterization of two putative nuclear localization signals (NLS) in the DNA-binding protein MUCKS. BBA - Molecular Cell Research. ISSN 0167-4889. 1773, s. 1398–1406. doi: 10.1016/j.bbamcr.2007.05.013. Fulltekst i vitenarkiv
• Grundt, Kirsten; Haga, Ingvild Vågslid; Aleporou-Marinou, V; Drosos, Y; Wanvik, Birgit & Østvold, Anne Carine (2004). Characterization of the NUCKS gene on human chromosome 1q32.1 and the presence of a homologous gene in different species. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 323, s. 796–801. doi: 10.1016/j.bbrc.2004.08.153. Fulltekst i vitenarkiv
• Grundt, Kirsten; Skjeldal, Lars; Anthonsen, Henrik W.; Skauge, Tormod; Huitfeldt, Henrik & Østvold, Anne Carine (2002). A putative DNA-binding domain in the NUCKS protein. Archives of Biochemistry and Biophysics. ISSN 0003-9861. 407(2), s. 168–175. Fulltekst i vitenarkiv

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• Østvold, Anne Carine & Grundt, Kirsten (2008). NUCKS expression is correlated with early onset of breast adenocarcinomas. Histopathology. ISSN 0309-0167. 53, s. 34–35. Fulltekst i vitenarkiv
• Skjeldal, Lars; Grundt, Kirsten; Østvold, Anne Carine; Anthonsen, Henrik W. & Skauge, Tormod (2003). NMR and graphic modeling of a protein-DNA complex. Fulltekst i vitenarkiv

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