Faglige interesser
- Immunologi
- Molekylærbiologi
- Genetikk
- Evolusjon
Undervisning
- Forelesninger, immunology, Medisin, 1. semester
- Forelesninger og PBL, Cellebiologi, Medisin, 2. semester
Bakgrunn
- Grunnla Immunbiologisk laboratorium (sammen med kollega Bent Rolstad og ingeniør Wendi Jensen)
- Professor, Anatomisk Institutt (nå Avdeling for Anatomi, Institutt for medisinske basalfag) 1992 -
- Vitenskapelig assistent, amanuensis, 1. amanuensis, Anatomisk Institutt, 1973 - 1992
- Dr. med. 1983
- Autorisert lege, 1973
- Turnustjeneste 1971 - 73
- Cand med, Oslo Universitet, 1971
Verv
- Prodekan for forskning, Det medisinske Fakultet, UiO, 2007 - 2010. Som forskningsdaken ex officio medlem og leder for ulike styrer, utvalg og komiteer
- Leder for Anatomisk Institutt, 2000 - 2003
- Medlem av styret for Bioteknologisenteret ved UiO, 1990 - 97
- Medlem av Fakultetsstyret, 1980 - 5 og 1993 - 7
Samarbeid
- Erik Dissen
- Michael R. Daws
Emneord:
Immunologi,
Molekylærbiologi,
Evolusjon,
Genetikk og epidemiologi
Publikasjoner
-
Blankson, Vera; Lobato Pascual, Ana; Sæther, Per Christian; Fossum, Sigbjørn; Dissen, Erik & Daws, Michael Rory
(2022).
Human macrophage C-type lectin forms a heteromeric receptor complex with Mincle but not Dectin-2.
Scandinavian Journal of Immunology.
ISSN 0300-9475.
95(5).
doi:
10.1111/sji.13149.
Fulltekst i vitenarkiv
-
Daws, Michael Rory; Nakken, Britt; Lobato Pascual, Ana; Regis, Josien; Dissen, Erik & Fossum, Sigbjørn
(2019).
Dendritic Cell Activating Receptor 1 (DCAR1) Associates With FcεRIγ and Is Expressed by Myeloid Cell Subsets in the Rat .
Frontiers in Immunology.
ISSN 1664-3224.
10.
doi:
10.3389/fimmu.2019.01060.
-
Daws, Michael Rory; Nakken, Britt; Lobato, Pascual Ana; Josien, Regis; Dissen, Erik & Fossum, Sigbjørn
(2019).
Dendritic Cell Activating Receptor 1 (DCAR1) Associates With Fc?RI? and Is Expressed by Myeloid Cell Subsets in the Rat.
Frontiers in Immunology.
ISSN 1664-3224.
10.
doi:
10.3389/fimmu.2019.01060.
Fulltekst i vitenarkiv
Vis sammendrag
Dendritic cell activating receptor-1 (DCAR1) is a cell-surface receptor encoded by the Antigen Presenting Lectin-like gene Complex (APLEC). We generated a mouse monoclonal antibody against rat DCAR1, and used this to characterize receptor expression and function. Rat DCAR1 was expressed on minor subsets of myeloid cells in lymphoid tissue, but was uniformly expressed at a high level by eosinophils, and at a low level by neutrophils. It was expressed by eosinophils in the peritoneal cavity and the lamina propria of the gut, and by subsets of macrophages or dendritic cells at these sites. Polarization of peritoneal macrophages showed that DCAR1 expression was absent on M1 macrophages, and increased on M2 macrophages. DCAR1 could be expressed as a homodimer and its associated with the activating adaptor protein FcεRIγ. This association allowed efficient phagocytosis of antibody-coated beads. Additionally, cross-linking of DCAR1 on the surface of rat eosinophils lead to production of reactive oxygen species. These data show that DCAR1 is an activating receptor. Its expression on M2 macrophages and eosinophils suggests that it may play a role in the immune response to parasites.
-
Thiruchelvam-Kyle, Lavanya; Hoelsbrekken, Sigurd Erik; Sæther, Per Christian; Bjørnsen, Elisabeth Gyllensten; Pende, Daniela & Fossum, Sigbjørn
[Vis alle 8 forfattere av denne artikkelen]
(2017).
The Activating Human NK Cell Receptor KIR2DS2 Recognizes a β2-Microglobulin–Independent Ligand on Cancer Cells.
Journal of Immunology.
ISSN 0022-1767.
198(7),
s. 2556–2567.
doi:
10.4049/jimmunol.1600930.
-
Lobato Pascual, Ana; Sæther, Per Christian; Fossum, Sigbjørn; Dissen, Erik & Daws, Michael Rory
(2013).
Mincle, the receptor for mycobacterial cord factor, forms a functional receptor complex with MCL and FcεRI-γ.
European Journal of Immunology.
ISSN 0014-2980.
43(12),
s. 3167–3174.
doi:
10.1002/eji.201343752.
-
-
Sæther, Per Christian; Hoelsbrekken, Sigurd Erik; Fossum, Sigbjørn & Dissen, Erik
(2011).
Rat and Mouse CD94 Associate Directly with the Activating Transmembrane Adaptor Proteins DAP12 and DAP10 and Activate NK Cell Cytotoxicity.
Journal of Immunology.
ISSN 0022-1767.
187(12),
s. 6365–6373.
doi:
10.4049/jimmunol.1102345.
-
Fossum, Sigbjørn; Sæther, Per Christian; Vaage, John Torgils; Daws, Michael Rory & Dissen, Erik
(2011).
Paired opposing leukocyte receptors recognizing rapidly evolving ligands are subject to homogenization of their ligand binding domains.
Immunogenetics.
ISSN 0093-7711.
63(12),
s. 809–820.
doi:
10.1007/s00251-011-0553-5.
-
Kveberg, Lise; Dai, Kezheng; Inngjerdingen, Marit; Brooks, Colin G. ; Fossum, Sigbjørn & Vaage, John Torgils
(2011).
Phylogenetic and functional conservation of the NKR-P1F and NKR-P1G receptors in rat and mouse.
Immunogenetics.
ISSN 0093-7711.
63(7),
s. 429–436.
doi:
10.1007/s00251-011-0520-1.
-
-
Flornes, Line Mari; Nylenna, Øyvind; Sæther, Per Christian; Daws, Michael Rory; Dissen, Erik & Fossum, Sigbjørn
(2010).
The complete inventory of receptors encoded by the rat natural killer cell gene complex.
Immunogenetics.
ISSN 0093-7711.
62(8),
s. 521–530.
doi:
10.1007/s00251-010-0455-y.
-
Kveberg, Lise; Dai, Kezheng; Westgaard, Ingunn Hagen; Daws, Michael Rory; Fossum, Sigbjørn & Naper, Christian
[Vis alle 7 forfattere av denne artikkelen]
(2009).
Two major groups of rat NKR-P1 receptors can be distinguished based on chromosomal localization, phylogenetic analysis and Clr ligand binding.
European Journal of Immunology.
ISSN 0014-2980.
39(2),
s. 541–551.
doi:
10.1002/eji.200838891.
-
Dissen, Erik; Hoelsbrekken, Sigurd Erik; Sæther, Per Christian & Fossum, Sigbjørn
(2008).
NK cell receptors in rodents and cattle.
Seminars in Immunology.
ISSN 1044-5323.
20(6),
s. 369–375.
-
Sæther, Per Christian; Westgaard, Ingunn Hagen; Hoelsbrekken, Sigurd Erik; Benjamin, J; Lanier, LL & Fossum, Sigbjørn
[Vis alle 7 forfattere av denne artikkelen]
(2008).
KLRE/I1 and KLRE/I2: A novel pair of heterodimeric receptors that inversely regulate NK cell cytotoxicity.
Journal of Immunology.
ISSN 0022-1767.
181,
s. 3177–3182.
-
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Hoelsbrekken, Sigurd Erik; Fossum, Sigbjørn & Dissen, Erik
(2005).
Molecular cloning of LILRC1 and LILRC2 in the mouse and the rat, two novel immunoglobulin-like receptors encoded by the leukocyte receptor gene complex.
Immunogenetics.
ISSN 0093-7711.
57(7),
s. 479–486.
-
Nylenna, Øyvind; Naper, Christian; Vaage, John Torgils; Woon, P.Y.; Gauguier, D & Dissen, Erik
[Vis alle 7 forfattere av denne artikkelen]
(2005).
The genes and gene organization of the Ly49 region of the rat natural killer cell gene complex.
European Journal of Immunology.
ISSN 0014-2980.
35.
-
Bryceson, Yenan T.; Torgersen, Knut Martin; Inngjerdingen, Marit; Berg, Siri Fuglem; Hoelsbrekken, Sigurd Erik & Fossum, Sigbjørn
[Vis alle 7 forfattere av denne artikkelen]
(2005).
The rat orthologue to the inhibitory receptor gp49B is expressed by neutrophils and monocytes, but not by NK cells or mast cells.
European Journal of Immunology.
ISSN 0014-2980.
35(4).
-
Sæther, Per Christian; Westgaard, Ingunn Hagen; Flornes, Line Marie; Hoelsbrekken, Sigurd Erik; Ryan, James C. & Fossum, Sigbjørn
[Vis alle 7 forfattere av denne artikkelen]
(2005).
Molecular cloning of KLRI1 and KLRI2, a novel pair of lectin-like natural killer-cell receptors with opposing signalling motifs.
Immunogenetics.
ISSN 0093-7711.
56.
-
Westgaard, Ingunn Hagen; Berg, Siri F.; Vaage, John Torgils; Wang, Lawrence L; Yokoyama, Wayne M. & Dissen, Erik
[Vis alle 7 forfattere av denne artikkelen]
(2004).
Rat NKp46 activates natural killer cell cytotoxicity and is associated with Fc{epsilon}RI{gamma} and CD3{zeta}.
Journal of Leukocyte Biology.
ISSN 0741-5400.
76,
s. 1200–1206.
doi:
10.1189/jlb.0903428.
Vis sammendrag
NKp46 has been identified in the human,
rat, mouse, monkey, and cattle. We have
generated a monoclonal antibody, WEN23, against
rat NKp46. By flow cytometry, NKp46 is expressed
by all natural killer (NK) cells but not by T
cells, B cells, granulocytes, monocytes, dendritic
cells, or macrophages. Thus, NKp46/WEN23 is
the first NK cell-specific marker in the rat. In a
redirected lysis assay, preincubation of the effector
cells with WEN23 augmented lysis of the Fc receptor
(FcR)+ murine tumor target cells, indicating
that NKp46 is an activating NK cell receptor.
Moreover, preincubation of the effector cells with
WEN23 F(ab')2 fragments reduced killing of target
cells, confirming the activating function of NKp46
and indicating that the mouse tumor target cells
express a ligand for rat NKp46. Lysis of FcR-
mouse and human tumor target cells was reduced
after incubation of effector cells with WEN23, suggesting
that rat NKp46 recognizes a ligand that is
conserved between primates and rodents. By Western
blot and immunoprecipitation using WEN23,
NKp46 is expressed as a monomer of ~47 kDa in
interleukin-2-activated NK cells. The immunoreceptor
tyrosine-based activation motif bearing
adaptor proteins CD3{zeta} and the {gamma} chain of FcRI for
IgE (Fc{epsilon}RI{gamma}) with NKp46 from lysates of NK cells,
indicating that rat NKp46 activates NK cell cytotoxicity
by similar pathways as CD16.
-
Flornes, Line Marie; Bryceson, Yenan T.; Spurkland, Anne; Lorentzen, JC; Dissen, Erik & Fossum, Sigbjørn
(2004).
Identification of lectin-like receptors expressed by antigen presenting cells and neutrophils and their mapping to a novel gene complex.
Immunogenetics.
ISSN 0093-7711.
56,
s. 506–517.
Vis sammendrag
In an experimental rat model, we recently mapped an arthritis susceptibility locus to the distal part of Chromosome 4 containing genes predicted to encode C-type lectin superfamily (CLSF) receptors. Here we report the cDNA cloning and positional arrangement of these receptor genes, which represent rat orthologues to human Mincle and DCIR and to mouse MCL and Dectin-2, as well as four novel receptors DCIR2, DCIR3, DCIR4 and DCAR1, not previously reported in other species. We furthermore report the cDNA cloning of human Dectin-2 and MCL, and of the mouse orthologues to the novel rat receptors. Similar to the killer-cell lectin-like receptors (KLR) some of these receptors exhibit structural features suggesting that they regulate leukocyte reactivity; e.g., human DCIR and rodent DCIR1 and DCIR2 carry an immunoreceptor tyrosine-based inhibitory motif (ITIM), predicting inhibitory function, and conversely, in all three species Mincle has a positively charged amino acid in the transmembrane region, suggesting activating function. Sequence comparisons show that the receptors form a discrete family, more closely related to group II CLSF receptors than to the group V KLR. Their distance to the KLR is underscored by their preservation of evolutionary conserved calcium/saccharide binding residues, present in group II and lacking in group V CLSF and their cellular expression patterns, with most of the genes preferentially expressed by professional antigen-presenting cells (dendritic cells, macrophages and B cells) and neutrophils. In all three species, the genes map together, forming an evolutionary conserved gene complex, which we call the antigen presenting lectin-like receptor complex (APLEC).
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Westgaard, Ingunn Hagen; Dissen, Erik; Torgersen, Knut Martin; Lazetic, Sascha; Lanier, Lewis L. & Phillips, JH
[Vis alle 7 forfattere av denne artikkelen]
(2003).
The lectin-like receptor KLRE1 inhibits natural killer cell cytotoxicity.
Journal of Experimental Medicine (JEM).
ISSN 0022-1007.
197(11),
s. 1551–1561.
-
Hoelsbrekken, Sigurd E.; Nylenna, Øyvind; Sæther, Per Christian; Slettedal, Imer Ö; Ryan, James C. & Fossum, Sigbjørn
[Vis alle 7 forfattere av denne artikkelen]
(2003).
Cutting edge: molecular cloning of a killer cell Ig-like receptor in the mouse and rat.
Journal of Immunology.
ISSN 0022-1767.
170(5),
s. 2259–2263.
-
Ribbhammar, Ulrica; Flornes, Line Marie; Bäckdahl, Liselotte; Luthman, Holger; Fossum, Sigbjørn & Lorentzen, Johnny
(2003).
High resolution mapping of an arthritis susceptibility locus on rat chromosome 4, and characterization of regulated phenotypes.
Human Molecular Genetics.
ISSN 0964-6906.
12,
s. 2087–2096.
-
Berg, Siri Fuglem; Westgaard, Ingunn Hagen; Fossum, Sigbjørn & Dissen, Erik
(1999).
A rat gene homologous to human granule membrane protein 17 is expressed by natural killer cells, CD8+ T cells and a mast cell line.
Immunogenetics.
ISSN 0093-7711.
29,
s. 815–818.
-
Berg, Siri Fuglem; Fossum, Sigbjørn & Dissen, Erik
(1999).
NILR-1, a novel immunoglobulin-like receptor expressed by neutrophilic granulocytes, is encoded by a leukocyte receptor gene complex on rat chromosome 1.
European Journal of Immunology.
ISSN 0014-2980.
29,
s. 2000–2006.
-
Berg, Siri Fuglem; Dissen, Erik; Westgaard, Ingunn Hagen & Fossum, Sigbjørn
(1998).
Two genes in the rat homologous to human NKG2.
European Journal of Immunology.
ISSN 0014-2980.
28,
s. 444–450.
-
Berg, Siri Fuglem; Dissen, Erik; Westgaard, Ingunn Hagen & Fossum, Sigbjørn
(1998).
Molecular characterization of rat NKR-P2, a lectin-like receptor expressed by NK cells and resting T cells.
International Immunology.
ISSN 0953-8178.
10,
s. 379–385.
-
Westgaard, Ingunn Hagen; Berg, Siri Fuglem; Ørstavik, Sigurd; Fossum, Sigbjørn & Dissen, Erik
(1998).
Identification of a human member of the Ly-49 multigene family.
European Journal of Immunology.
ISSN 0014-2980.
28,
s. 1839–1846.
-
Dissen, Erik; Berg, Siri Fuglem; Westgaard, Ingunn Hagen & Fossum, Sigbjørn
(1997).
Molecular characterization of a gene in the rat homologous to human CD94.
European Journal of Immunology.
ISSN 0014-2980.
27,
s. 2080–2086.
-
Dissen, Erik; Ryan, James C.; Seaman, W. E. & Fossum, Sigbjørn
(1996).
An autosomal dominant locus, Nka, mapping to the Ly-49 region of a rat natural killer (NK) gene complex, controls NK cell lysis of allogeneic lymphocytes.
Journal of Experimental Medicine (JEM).
ISSN 0022-1007.
183,
s. 2197–2207.
-
Dissen, Erik & Fossum, Sigbjørn
(1996).
Chromosomal localization of the genes encoding rat CD4, CD8 and CD8.
Immunogenetics.
ISSN 0093-7711.
44,
s. 312–314.
-
Dissen, Erik; Vaage, John T.; Tasken, Kjetil; Jahnsen, Tore; Rolstad, Bent & Fossum, Sigbjørn
(1990).
Alloreactive lymphokine-activated killer cells from athymic nude rats do not express CD3-associated alpha/beta or gamma/delta T cell receptors.
International Immunology.
ISSN 0953-8178.
2(5),
s. 453–461.
Se alle arbeider i Cristin
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Nakken, Britt; Jensen, Wendi; Szodoray, Peter; Daws, Michael Rory & Fossum, Sigbjørn
(2011).
Role of the C-Type Lectin-Like Receptor Dcar1 in Oil Induced Arthritis.
Scandinavian Journal of Immunology.
ISSN 0300-9475.
73(4),
s. 375–376.
-
Lobato Pascual, Ana; Sæther, Per Christian; Fossum, Sigbjørn & Daws, Michael Rory
(2010).
Exploring expression and function of the rat Macrophage C-type Lectin, Clecsf8.
Vis sammendrag
Innate immune response receptors play an important role in recognizing molecular patterns in pathogens and altered host cells. Among these are the C-type lectin-like receptors, which have been shown to recognize both carbohydrate structures as well as specific protein ligands. The Antigen Presenting cell LEctin-like receptor Complex, APLEC, is an evolutionary conserved gene cluster in the rat, mouse and human, which has been previously described as an immune response locus that influences autoimmunity. This gene complex has been linked to oil-induced arthritis in the rat, and an association has also been shown to rheumatoid arthritis in the man. The Macrophage C-type Lectin, Clecsf8, is a receptor encoded by APLEC. Preliminary data from our group has shown that MCL is upregulated in the arthritis susceptible DA strain, but not in the DA rats containing the arthritis-resistant PVG APLEC genes. We have developed in-house monoclonal antibodies against rat-Clecsf8 to examine the distribution and expression pattern of this receptor in a variety of organs. We have found that this protein is mainly expressed in the monocyte/macrophage lineage, neutrophils and dendritic cells. The presence of Clecsf8 in neutrophils and professional antigen presenting cells suggests a role of this receptor in the early stages of antigen recognition and a possible link to the initiation of the adaptive immunity response.
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Kveberg, Lise; Dai, Kezheng; Martinsen, Stine; Fossum, Sigbjørn & Vaage, John Torgils
(2009).
Phylogenetic and functional conservation of the NKR-P1G NK receptor in rat and mouse.
-
Kveberg, Lise; Fossum, Sigbjørn & Vaage, John Torgils
(2009).
Phylogenetic and functional conservation of the NKR-P1G receptor in rat and mouse.
-
Kveberg, Lise; Westgaard, Ingunn Hagen; Dai, Kezheng; Daws, Michael Rory; Fossum, Sigbjørn & Naper, Christian
[Vis alle 7 forfattere av denne artikkelen]
(2007).
The NKR-P1 family of receptors in rat recognizes C-type lectin-related molecules.
-
Kveberg, Lise; Westgaard, Ingunn Hagen; Dai, Kezheng; Daws, Michael Rory; Fossum, Sigbjørn & Naper, Christian
[Vis alle 7 forfattere av denne artikkelen]
(2007).
The NKR-P1 family of receptors in rat recognizes C-type lectin related molecules.
-
Rolstad, Bent & Fossum, Sigbjørn
(1999).
Natural killer celler, enfant terrible i immunologien.
NBS-nytt.
ISSN 0801-3535.
23,
s. 7–13.
-
Kristensen, Petter; Levy, Finn; Grahnstedt, Svein G; Martinsen, Aud; Schiel, Anja Elisabeth & Flornes, Line Mari
[Vis alle 9 forfattere av denne artikkelen]
(2011).
Oljer og helse - et pilotprosjekt. Kartlegging av oljeeksponering, genetiske faktorer og sykdom hos pasienter som ble utredet for mulig arbeidsrelatertsykdom.
Statens Arbeidsmiljøinstitutt.
ISSN 1502-0932.
Årgang 12(Nr. 1).
Se alle arbeider i Cristin
Publisert
13. apr. 2011 10:36
- Sist endret
14. mars 2012 15:19