Lene Alsøe

• Alsøe, Lene; Sarno, Antonio; Carracedo Huroz, Sergio; Domanska, Diana Ewa; Dingler, Felix; Lirussi, Lisa; Sengupta, Tanima; Tekin, Nuriye Basdag; Jobert, Laure; Alexandrov, Ludmil B.; Galashevskaya, Anastasia; Rada, Cristina; Sandve, Geir Kjetil; Rognes, Torbjørn; Krokan, Hans Einar & Nilsen, Hilde Loge (2017). Uracil Accumulation and Mutagenesis Dominated by Cytosine Deamination in CpG Dinucleotides in Mice Lacking UNG and SMUG1. Scientific Reports.  ISSN 2045-2322.  7(1) . doi: 10.1038/s41598-017-07314-5 Fulltekst i vitenarkiv.
• Holm, Kristine Lillebø; Syljuåsen, Randi G.; Hasvold, Grete; Alsøe, Lene; Nilsen, Hilde Loge; Ivanauskiene, Kristina; Collas, Philippe; Shaposhnikov, Sergey; Collins, Andrew Richard; Indrevær, Randi Larsen; Aukrust, Pål; Fevang, Børre & Blomhoff, Heidi Kiil (2017). TLR9 stimulation of B-cells induces transcription of p53 and prevents spontaneous and irradiation-induced cell death independent of DNA damage responses. Implications for Common variable immunodeficiency. PLoS ONE.  ISSN 1932-6203.  12(10) . doi: 10.1371/journal.pone.0185708 Fulltekst i vitenarkiv.
• Nguyen, Chinh Bkrong Thi Thuy; Alsøe, Lene; Lindvall, Jessica Margareta; Sulheim, Dag; Fagermoen, Frode Even; Winger, Anette; Kaarbø, Mari; Nilsen, Hilde Loge & Wyller, Vegard Bruun Bratholm (2017). Whole blood gene expression in adolescent chronic fatigue syndrome: An exploratory cross-sectional study suggesting altered B cell differentiation and survival. Journal of Translational Medicine.  ISSN 1479-5876.  15 . doi: 10.1186/s12967-017-1201-0 Fulltekst i vitenarkiv. Vis sammendrag

  Background Chronic fatigue syndrome (CFS) is a prevalent and disabling condition affecting adolescents. The pathophysiology is poorly understood, but immune alterations might be an important component. This study compared whole blood gene expression in adolescent CFS patients and healthy controls, and explored associations between gene expression and neuroendocrine markers, immune markers and clinical markers within the CFS group. Methods CFS patients (12–18 years old) were recruited nation-wide to a single referral center as part of the NorCAPITAL project. A broad case definition of CFS was applied, requiring 3 months of unexplained, disabling chronic/relapsing fatigue of new onset, whereas no accompanying symptoms were necessary. Healthy controls having comparable distribution of gender and age were recruited from local schools. Whole blood samples were subjected to RNA sequencing. Immune markers were blood leukocyte counts, plasma cytokines, serum C-reactive protein and immunoglobulins. Neuroendocrine markers encompassed plasma and urine levels of catecholamines and cortisol, as well as heart rate variability indices. Clinical markers consisted of questionnaire scores for symptoms of post-exertional malaise, inflammation, fatigue, depression and trait anxiety, as well as activity recordings. Results A total of 29 CFS patients and 18 healthy controls were included. We identified 176 genes as differentially expressed in patients compared to controls, adjusting for age and gender factors. Gene set enrichment analyses suggested impairment of B cell differentiation and survival, as well as enhancement of innate antiviral responses and inflammation in the CFS group. A pattern of co-expression could be identified, and this pattern, as well as single gene transcripts, was significantly associated with indices of autonomic nervous activity, plasma cortisol, and blood monocyte and eosinophil counts. Also, an association with symptoms of post-exertional malaise was demonstrated. Conclusion Adolescent CFS is characterized by differential gene expression pattern in whole blood suggestive of impaired B cell differentiation and survival, and enhanced innate antiviral responses and inflammation. This expression pattern is associated with neuroendocrine markers of altered HPA axis and autonomic nervous activity, and with symptoms of post-exertional malaise.

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• Alsøe, Lene (2018). APOBECs in colitis-associated inflammatory bowel disease.
• Alsøe, Lene; Wennerström, Anna Berit; Liu, Yan; Esbensen, Qin Ying; Bjørset, Randi; Penz, Christine; Øresland, Tom; Andersen, Solveig Norheim; Brackmann, Stephan & Nilsen, Hilde Loge (2018). Do APOBEC proteins contribute to genomic instability in colitis-associated colorectal cancer?.
• Alsøe, Lene; Sarno, Antonio; Carracedo Huroz, Sergio; Domanska, Diana Ewa; Dingler, Felix; Lirussi, Lisa; Sengupta, Tanima; Tekin, Nuriye Basdag; Jobert, Laure; Alexandrov, Ludmil B.; Galashevskaya, Anastasia; Rada, Christina; Sandve, Geir Kjetil; Rognes, Torbjørn; Krokan, Hans Einar & Nilsen, Hilde Loge (2017). Uracil Accumulation and Mutagenesis Dominated by Cytosine Deamination in CpG Dinucleotides in Mice Lacking UNG and SMUG1.
• Alsøe, Lene; Wennerstrøm, Anna; Liu, Yan; Esbensen, Qin Ying; Bjørseth, Randi; Penz, Christine; Øresland, Tom; Andersen, Solveig Norheim; Brackmann, Stephan & Nilsen, Hilde Loge (2017). Do APOBEC proteins contribute to genomic instability in colitis-associated colorectal cancer?.
• Alsøe, Lene; Wennerstrøm, Anna; Liu, Yan; Esbensen, Qin Ying; Bjørseth, Randi; Penz, Christine; Øresland, Tom; Andersen, Solveig Norheim; Nilsen, Hilde & Brackmann, Stephan (2017). Do APOBEC proteins contribute to genomic instability in colitis-associated colorectal cancer?.
• Nilsen, Hilde; Kroustallaki, Pinelopi; Alsøe, Lene; Huroz, Sergio C; Sarno, Antonio; Esbensen, Ying; Wang, Wei; Galashevskaya, Anastasia; Krokan, Hans Einar; Bjørås, Magnar & Gagos, Sarantis (2016). Telomere maintenance defects in mice deficient in SMUG1 uracil-DNA glycosylase.
• Sarno, Antonio; Alsøe, Lene; Carracedo, Sergio; Tekin, Nuriya Basdag; Gupta, Tanima Sen; Jobert, Laure; Galashevskaya, Anastasia; Krokan, Hans Einar & Nilsen, Hilde (2016). UNG and SMUG1 complement each other in DNA uracil excision and DNA 5-hydroxymethyluracil originates from 5-hydroxymethylcytosine deamination in mice.
• Alsøe, Lene & Gaudernack, Gustav (2010). Development of novel methods for immunoproteomics of cancer and high-throughput analysis of pancreatic cancer.

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