Nikolina Sekulic

• Stonyte, Vilte; Martîn, Ruth Martîn; Segura-Pena, Dario; Sekulic, Nikolina & Lopez Aviles, Sandra (2020). Requirement of PP2A-B56 Par1 for the Stabilization of the CDK Inhibitor Rum1 and Activation of APC/C Ste9 during Pre-Start G1 in S. pombe. iScience. ISSN 2589-0042. 23(5). doi: 10.1016/j.isci.2020.101063. Full text in Research Archive
• Ali Ahmad, Ahmad & Sekulic, Nikolina (2020). CENP-A nucleosome-a chromatin-embedded pedestal for the centromere: lessons learned from structural biology. Essays in Biochemistry. ISSN 0071-1365. 64(2), p. 205–221. doi: 10.1042/EBC20190074. Full text in Research Archive
• Ali Ahmad, Ahmad; Bilokapic, Silvija; Schafer, Ingmar B; Halic, Mario & Sekulic, Nikolina (2019). CENP‐C unwraps the human CENP‐A nucleosome through the H2A C‐terminal tail. EMBO Reports. ISSN 1469-221X. doi: 10.15252/embr.201948913. Full text in Research Archive
• Sekulic, Nikolina & Black, Ben E. (2016). Preparation of Recombinant Centromeric Nucleosomes and Formation of Complexes with Nonhistone Centromere Proteins. Methods in Enzymology. ISSN 0076-6879. 573, p. 67–96. doi: 10.1016/bs.mie.2016.01.014. Show summary

  Centromeres are present on each chromosome to direct proper segregation during cell division. The understanding of how the histone H3 variant, CENP-A, epigenetically marks the location of the centromere on the chromosome has been advanced, in part, through the study of histone complexes, nucleosomes, and nucleosomal complexes with nonhistone centromere proteins. In this chapter, we describe the preparation of recombinant versions of these complexes. The methodology is firmly rooted in classic nucleosome reconstitution methods, but we highlight the aspects of the preparations that diverge from those used for the methods established with canonical histones. We also provide a method for producing PCR-amplified nucleosomal DNA sequences in milligram quantities that is particularly useful for studies where multiple sequences and/or chemical modifications are desired. Lastly, we describe our approach to assemble and analyze a complex between the recombinant human CENP-A nucleosome and one of its binding partners, CENP-C.

• Avgousti, Daphne C.; Herrmann, Christin; Kulej, Katarzyna; Pancholi, Neha J.; Sekulic, Nikolina & Petrescu, Joana [Show all 17 contributors for this article] (2016). A core viral protein binds host nucleosomes to sequester immune danger signals. Nature. ISSN 0028-0836. 535(7610), p. 173–177. doi: 10.1038/nature18317.
• Falk, Samantha J.; Lee, Jaehyoun; Sekulic, Nikolina; Sennett, Michael A.; Lee, Tae-Hee & Black, Ben E. (2016). CENP-C directs a structural transition of CENP-A nucleosomes mainly through sliding of DNA gyres. Nature Structural & Molecular Biology. ISSN 1545-9993. 23(3), p. 204–208. doi: 10.1038/nsmb.3175.

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• Mojica Cortez, Natalia; Sekulic, Nikolina & Krengel, Ute (2020). UiO Structural Biology Core Facilities: Who Are We and How Can We Help You? .
• Sekulic, Nikolina; Cascella, Michele & Hovet, Oda (2020). Molecular Dynamics Studies on the Essential Mitotic Protein Kinase – Aurora B. Reprosentralen, University of Oslo.

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