Nikolina Sekulic

• Avgousti, Daphne C.; Herrmann, Christin; Kulej, Katarzyna; Pancholi, Neha J.; Sekulic, Nikolina; Petrescu, Joana; Molden, Rosalynn C.; Blumenthal, Daniel; Paris, Andrew J.; Reyes, Emigdio D.; Ostapchuk, Philomena; Hearing, Patrick; Seeholzer, Steven H.; Worthen, G. Scott; Black, Ben E.; Garcia, Benjamin A. & Weitzman, Matthew D. (2016). A core viral protein binds host nucleosomes to sequester immune danger signals. Nature.  ISSN 0028-0836.  535(7610), s 173- 177 . doi: 10.1038/nature18317
• Falk, Samantha J.; Lee, Jaehyoun; Sekulic, Nikolina; Sennett, Michael A.; Lee, Tae-Hee & Black, Ben E. (2016). CENP-C directs a structural transition of CENP-A nucleosomes mainly through sliding of DNA gyres. Nature Structural & Molecular Biology.  ISSN 1545-9993.  23(3), s 204- 208 . doi: 10.1038/nsmb.3175
• Sekulic, Nikolina & Black, Ben E. (2016). Preparation of Recombinant Centromeric Nucleosomes and Formation of Complexes with Nonhistone Centromere Proteins. Methods in Enzymology.  ISSN 0076-6879.  573, s 67- 96 . doi: 10.1016/bs.mie.2016.01.014 Show summary

  Centromeres are present on each chromosome to direct proper segregation during cell division. The understanding of how the histone H3 variant, CENP-A, epigenetically marks the location of the centromere on the chromosome has been advanced, in part, through the study of histone complexes, nucleosomes, and nucleosomal complexes with nonhistone centromere proteins. In this chapter, we describe the preparation of recombinant versions of these complexes. The methodology is firmly rooted in classic nucleosome reconstitution methods, but we highlight the aspects of the preparations that diverge from those used for the methods established with canonical histones. We also provide a method for producing PCR-amplified nucleosomal DNA sequences in milligram quantities that is particularly useful for studies where multiple sequences and/or chemical modifications are desired. Lastly, we describe our approach to assemble and analyze a complex between the recombinant human CENP-A nucleosome and one of its binding partners, CENP-C.

View all works in Cristin