Our research group is situated within the Department of Molecular Medicine at the Institute of Basic Medical Sciences, University of Oslo, and collaborates closely with the Department of Neurology at Akershus University Hospital, particularly with Trygve Holmøy's research team. We are dedicated to comprehensively mapping cellular and humoral immune responses in neuroinflammation. To achieve this, we utilize a broad spectrum of techniques including high-throughput sequencing, single-cell RNA sequencing, spectral flow cytometry, isoelectric focusing, immunoblotting, T-cell cloning, mass spectrometry, and bioinformatics.
Additionally, we have a profound interest in the genetics of immune receptor loci. In our pursuit to elucidate these genetic regions, we employ Oxford Nanopore long-read sequencing. Our approach allows for the phasing of haplotypes and also enables the resolution of complex structural variants, thereby enhancing our understanding of their complexities.
Projects
- B cells and immunoglobulins in the cerebrospinal fluid of patients with MS
- Genetic variants of the IGH locus
- T follicular helper cells in MS
Representative publications
Polak J, Wagnerberger JH, Torsetnes SB, Lindeman I, Høglund RA, Vartdal F, Sollid LM, Lossius A. Single-cell transcriptomics combined with proteomics of intrathecal IgG reveal transcriptional heterogeneity of oligoclonal IgG-secreting cells in multiple sclerosis. Frontiers in Cellular Neuroscience. 2023. https://doi.org/10.3389/fncel.2023.1189709
Lindeman I, Polak J, Qiao SW, Holmøy T, Høglund RA, Vartdal F, Berg-Hansen P, Sollid LM, Lossius A. Stereotyped B-cell responses are linked to IgG constant region polymorphisms in multiple sclerosis. European Journal of Immunology. 2022. https://doi.org/10.1002/eji.202149576
Høglund RA, Bremel RD, Homan EJ, Torsetnes SB, Lossius A*, Holmøy T*. CD4+ T Cells in the Blood of MS Patients Respond to Predicted Epitopes From B cell Receptors Found in Spinal Fluid. Frontiers in Immunology. 2020; 11: 598. *Shared senior authorship. https://doi.org/10.3389/fimmu.2020.00598
Tomescu-Baciu A, Johansen JN, Holmøy T, Greiff V, Stensland M, de Souza GA, Vartdal F, Lossius A. Persistence of intrathecal oligoclonal B cells and IgG in multiple sclerosis. Journal of Neuroimmunology. 2019; 333: 576966. https://doi.org/10.1016/j.jneuroim.2019.576966
Tomescu-Baciu A, Vartdal F, Holmøy T, Vedeler CA, Lossius A. G1m1 predominance of intrathecal virus-specific antibodies in multiple sclerosis. Annals of Clinical and Translational Neurology. 2018; 5(10): 1303-1309. https://doi.org/10.1002/acn3.642
Lossius A, Tomescu-Baciu A, Holmøy T, Vedeler CA, Røsjø E, Lorentzen ÅR, Casetta I, Vartdal F. Selective intrathecal enrichment of G1m1-positive B cells in multiple sclerosis. Annals of Clinical and Translational Neurology. 2017; 4(10):756-761. https://doi.org/10.1002/acn3.451