Magnar Bjørås

• Bugaj, Anna Maria; Kunath, Nicolas; Saasen, Vidar Langseth; Fernandez-Berrocal, Marion S.; Vankova, Ana & Sætrom, Pål [Vis alle 8 forfattere av denne artikkelen] (2024). Dissecting gene expression networks in the developing hippocampus through the lens of NEIL3 depletion. Progress in Neurobiology. ISSN 0301-0082. 235. doi: 10.1016/j.pneurobio.2024.102599.
• Ianevski, Aleksandr; Frøysa, Irene Trøen; Lysvand, Hilde; Calitz, Carlemi; Smura, Teemu & Nilsen, Hans-Johnny [Vis alle 18 forfattere av denne artikkelen] (2024). The combination of pleconaril, rupintrivir, and remdesivir efficiently inhibits enterovirus infections in vitro, delaying the development of drug-resistant virus variants. Antiviral Research. ISSN 0166-3542. 224. doi: 10.1016/j.antiviral.2024.105842.
• Neurauter, Christine Gran; Pannone, Marco; Sousa, Mirta; Wang, Wei; Kusnierczyk, Anna & Luna, Luisa [Vis alle 9 forfattere av denne artikkelen] (2024). Enhanced glutathione levels confer resistance to apoptotic and ferroptotic programmed cell death in NEIL DNA glycosylase deficient HAP1 cells. Free Radical Biology & Medicine. ISSN 0891-5849. 213, s. 470–487. doi: 10.1016/j.freeradbiomed.2024.01.037.
• Bordin, Diana Lilian; Grooms, Kayla Mae; Montaldo, Nicola Pietro; Fordyce Martin, Sarah L.; Sætrom, Pål & Samson, Leona D. [Vis alle 8 forfattere av denne artikkelen] (2024). Loss of alkyladenine DNA glycosylase alters gene expression in the developing mouse brain and leads to reduced anxiety and improved memory. DNA Repair. ISSN 1568-7864. 135. doi: 10.1016/j.dnarep.2024.103632.
• Benjakul, Sopisa; Anthi, Aina Karen; Kolderup, Heidi Anette; Vaysburd, Marina; Lode, Heidrun Elisabeth & Mallery, D [Vis alle 20 forfattere av denne artikkelen] (2023). A pan-SARS-CoV-2-specific soluble angiotensin-converting enzyme 2-albumin fusion engineered for enhanced plasma half-life and needle-free mucosal delivery. PNAS Nexus. ISSN 2752-6542. 2(12), s. 1–15. doi: 10.1093/pnasnexus/pgad403. Fulltekst i vitenarkiv
• Starheim, Eirin Johannessen; Ravlo, Erlend; Schjølberg, Jørn-Ove; Solvang, Vanessa; Wang, Wei & Scrimgeour, Nathan Robert [Vis alle 12 forfattere av denne artikkelen] (2023). NAxtra magnetic nanoparticles for low-cost, efficient isolation of mammalian DNA and RNA. Scientific Reports. ISSN 2045-2322. 13(1), s. 1–13. doi: 10.1038/s41598-023-46868-5. Fulltekst i vitenarkiv
• Bali, Nesrine; Brennhaug, Svein Jonsson; Bjørås, Magnar; Bandyopadhyay, Sulalit & Manaf, Adeel (2023). Optimized synthesis of polyacrylic acid-coated magnetic nanoparticles for high-efficiency DNA isolation and size selection. RSC Advances. ISSN 2046-2069. 13(42), s. 29109–29120. doi: 10.1039/d3ra04687g. Fulltekst i vitenarkiv
• Lin, Xiaolin; Wang, Wei; Yang, Mingyi; Damseh, Nadirah; Sousa, Mirta & Jacob, Fadi [Vis alle 29 forfattere av denne artikkelen] (2023). A loss-of-function mutation in human Oxidation Resistance 1 disrupts the spatial–temporal regulation of histone arginine methylation in neurodevelopment. Genome Biology. ISSN 1465-6906. 24(1). doi: 10.1186/s13059-023-03037-1. Fulltekst i vitenarkiv
• Ravlo, Erlend; Sousa, Mirta; Andresen, Lise Lima; Holberg-Petersen, Mona; Klundby, Ingvild & Aas, Per Arne [Vis alle 14 forfattere av denne artikkelen] (2023). A fast, low-cost, robust and high-throughput method for viral nucleic acid isolation based on NAxtra magnetic nanoparticles. Scientific Reports. ISSN 2045-2322. 13(1), s. 1–7. doi: 10.1038/s41598-023-38743-0. Fulltekst i vitenarkiv
• Yang, Mingyi; Ali, Omer; Bjørås, Magnar & Wang, Junbai (2023). Identifying functional regulatory mutation blocks by integrating genome sequencing and transcriptome data. iScience. ISSN 2589-0042. 26(8), s. 1–23. doi: 10.1016/j.isci.2023.107266. Fulltekst i vitenarkiv
• Ramos Alonso, Lucia; Holland, Petter; Le Gras, Stéphanie; Zhao, Xu; Jost, Bernard & Bjørås, Magnar [Vis alle 9 forfattere av denne artikkelen] (2023). Mitotic chromosome condensation resets chromatin to safeguard transcriptional homeostasis during interphase. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 120(4). doi: 10.1073/pnas.2210593120. Fulltekst i vitenarkiv
• Sæbø, Ingvill Pedersen; Bjørås, Magnar; Franzyk, Henrik; Helgesen, Emily & Booth, James Alexander (2023). Optimization of the Hemolysis Assay for the Assessment of Cytotoxicity. International Journal of Molecular Sciences. ISSN 1661-6596. 24(3), s. 1–20. doi: 10.3390/ijms24032914. Fulltekst i vitenarkiv
• Pinto, Sneha M.; Subbannayya, Yashwanth; Kim, Hera; Hagen, Lars; Górna, Maria W & Nieminen, Anni I. [Vis alle 10 forfattere av denne artikkelen] (2022). Multi-OMICs landscape of SARS-CoV-2-induced host responses in human lung epithelial cells. iScience. ISSN 2589-0042. 26(1), s. 1–33. doi: 10.1016/j.isci.2022.105895. Fulltekst i vitenarkiv
• Olsen, Maria Belland; Huse, Camilla; Sousa, Mirta; Murphy, Sarah Louise Mikalsen; Sarno, Antonio & Obermann, Tobias Sebastian [Vis alle 20 forfattere av denne artikkelen] (2022). DNA Repair Mechanisms are Activated in Circulating Lymphocytes of Hospitalized Covid-19 Patients. Journal of Inflammation Research. ISSN 1178-7031. 15, s. 6629–6644. doi: 10.2147/JIR.S379331. Fulltekst i vitenarkiv
• Ianevski, Aleksandr; Ahmad, Shahzaib; Anunnitipat, Kraipit; Oksenych, Valentyn; Zusinaite, Eva & Tenson, Tanel [Vis alle 8 forfattere av denne artikkelen] (2022). Seven classes of antiviral agents. Cellular and Molecular Life Sciences (CMLS). ISSN 1420-682X. 79(12). doi: 10.1007/s00018-022-04635-1.
• Kaarbø, Mari; Yang, Mingyi; Hov, Johannes Espolin Roksund; Holm, Kristian; Sousa, Mirta & Macpherson, Magnhild Eide [Vis alle 15 forfattere av denne artikkelen] (2022). Duodenal inflammation in common variable immunodeficiency has altered transcriptional response to viruses. Journal of Allergy and Clinical Immunology. ISSN 0091-6749. doi: 10.1016/j.jaci.2022.09.029. Fulltekst i vitenarkiv Vis sammendrag

  Background A substantial proportion of common variable immunodeficiency (CVID) patients has duodenal inflammation of largely unknown etiology. However, because of its histologic similarities with celiac disease, gluten sensitivity has been proposed as a potential mechanism. Objective We aimed to elucidate the role of the duodenal microenvironment in the pathogenesis of duodenal inflammation in CVID by investigating the transcriptional, proteomic, and microbial signatures of duodenal biopsy samples in CVID. Methods DNA, total RNA, and protein were isolated from snap-frozen pieces of duodenal biopsy samples from CVID (with and without duodenal inflammation), healthy controls, and patients with celiac disease (untreated). RNA sequencing, mass spectrometry–based proteomics, and 16S ribosomal DNA sequencing (bacteria) were then performed. Results CVID separated from controls in regulation of transcriptional response to lipopolysaccharide and cellular immune responses. These differences were independent of mucosal inflammation. Instead, CVID patients with duodenal inflammation displayed alterations in transcription of genes involved in response to viral infections. Four proteins were differently regulated between CVID patients and healthy controls—DBNL, TRMT11, GCHFR, and IGHA2—independent of duodenal inflammation. Despite similar histology, there were major differences in CVID with duodenal inflammation and celiac disease both at the RNA and protein level. No significant difference was observed in the bacterial gut microbial signature between CVID, celiac, and healthy controls. Conclusion Our findings suggest the existence of altered functions of the duodenal epithelium, particularly in response to lipopolysaccharide and viruses. The latter finding was related to duodenal inflammation, suggesting that viruses, not gluten sensitivity, could be related to duodenal inflammation in CVID. Key words CVID RNA sequencing proteomics microbiome gut microbiota microbiota gastrointestinal tract duodenum celiac disease Primary immunodeficiency IgA

• Ravlo, Erlend; Evensen, Lasse; Sanson, Gorm; Hildonen, Siri; Ianevski, Aleksandr & Skjervold, Per Olav [Vis alle 12 forfattere av denne artikkelen] (2022). Antiviral Immunoglobulins of Chicken Egg Yolk for Potential Prevention of SARS-CoV-2 Infection. Viruses. ISSN 1999-4915. 14(10). doi: 10.3390/v14102121. Fulltekst i vitenarkiv
• Egiazarian, Milena Armenovna; Strømstad, Silje; Sakshaug, Teri; Núñez Nescolarde, Ana Beatriz; Bethge, Nicole & Bjørås, Magnar [Vis alle 7 forfattere av denne artikkelen] (2022). Age- and sex-dependent effects of DNA glycosylase Neil3 on amyloid pathology, adult neurogenesis, and memory in a mouse model of Alzheimer's disease. Free Radical Biology & Medicine. ISSN 0891-5849. 193(Pt 2), s. 685–693. doi: 10.1016/j.freeradbiomed.2022.11.020. Fulltekst i vitenarkiv
• Ianevski, Aleksandr; Zusinaite, Eva; Tenson, Tanel; Oksenych, Valentyn; Wang, Wei & Afset, Jan Egil [Vis alle 8 forfattere av denne artikkelen] (2022). Novel Synergistic Anti-Enteroviral Drug Combinations. Viruses. ISSN 1999-4915. 14(9). doi: 10.3390/v14091866. Fulltekst i vitenarkiv
• Olsen, Maria Belland; Sannes, Ann-Christin; Yang, Kuan; Nielsen, Morten Birkeland; Einarsen, Ståle Valvatne & Christensen, Jan Olav [Vis alle 9 forfattere av denne artikkelen] (2022). Mapping of pituitary stress-induced gene regulation connects Nrcam to negative emotions. iScience. ISSN 2589-0042. 25(9). doi: 10.1016/j.isci.2022.104953. Fulltekst i vitenarkiv Vis sammendrag

  Environmental stressors such as repeated social defeat may initiate powerful activation of subconscious parts of the brain. Here, we examine the consequences of such stress (induced by resident-intruder paradigm) on the pituitary gland. In male stressed vs. control rats, by RNA- and bisulfite DNA sequencing, we found regulation of genes involved in neuron morphogenesis and communication. Among these, Neuronal cell adhesion molecule (Nrcam) showed reduced transcription and reduced DNA methylation in a region corresponding to intron 1 in human NRCAM. Also, genetic variability in this area was associated with altered stress response in male humans exposed to repeated social defeat in the form of abusive supervision. Thus, our data show that the pituitary gene expression may be affected by social stress and that genetic variability in NRCAM intron 1 region influences stress-induced negative emotions. We hope our shared datasets will facilitate further exploration of the motions triggered by social stressors.

• Quiles-Jiménez, Ana M T; Dahl, Tuva Børresdatter; Bjørås, Magnar; Alseth, Ingrun; Halvorsen, Bente & Gregersen, Ida (2022). Epitranscriptome in Ischemic Cardiovascular Disease: Potential Target for Therapies. Stroke. ISSN 0039-2499. 53(6), s. 2114–2122. doi: 10.1161/STROKEAHA.121.037581.
• Kennedy, Lauritz Hagen; Glesaaen, Emilie Rylund; Palibrk, Vuk; Pannone, Marco; Wang, Wei & Al-Jabri, Ali [Vis alle 13 forfattere av denne artikkelen] (2022). Lactate receptor HCAR1 regulates neurogenesis and microglia activation after neonatal hypoxia-ischemia. eLIFE. ISSN 2050-084X. 11. doi: 10.7554/eLife.76451. Fulltekst i vitenarkiv
• Ianevski, Aleksandr; Simonsen, Ronja Meyer; Myhre, Vegard; Tenson, Tanel; Oksenych, Valentyn & Bjørås, Magnar [Vis alle 7 forfattere av denne artikkelen] (2022). DrugVirus.info 2.0: an integrative data portal for broad-spectrum antivirals (BSA) and BSA-containing drug combinations (BCCs). Nucleic Acids Research (NAR). ISSN 0305-1048. 50(W1), s. W272–W275. doi: 10.1093/nar/gkac348. Fulltekst i vitenarkiv
• Ianevski, Aleksandr; Yao, Rouan; Simonsen, Ronja Meyer; Myhre, Vegard; Ravlo, Erlend & Kaynova, Gerda D. [Vis alle 18 forfattere av denne artikkelen] (2022). Mono- and combinational drug therapies for global viral pandemic preparedness. iScience. ISSN 2589-0042. 25(4). doi: 10.1016/j.isci.2022.104112. Fulltekst i vitenarkiv
• Karlsen, Tom Rune; Olsen, Maria Belland; Kong, Xiang Yi; Yang, Kuan; Quiles-Jiménez, Ana M T & Kroustallaki, Pinelopi [Vis alle 15 forfattere av denne artikkelen] (2022). NEIL3-deficient bone marrow displays decreased hematopoietic capacity and reduced telomere length. Biochemistry and Biophysics Reports. ISSN 2405-5808. 29. doi: 10.1016/j.bbrep.2022.101211. Fulltekst i vitenarkiv
• Ali, Omer; Farooq, Amna; Yang, Mingyi; Jin, Victor X.; Bjørås, Magnar & Wang, Junbai (2022). abc4pwm: affinity based clustering for position weight matrices in applications of DNA sequence analysis. BMC Bioinformatics. ISSN 1471-2105. 23(1). doi: 10.1186/s12859-022-04615-z. Fulltekst i vitenarkiv Vis sammendrag

  Background Transcription factor (TF) binding motifs are identified by high throughput sequencing technologies as means to capture Protein-DNA interactions. These motifs are often represented by consensus sequences in form of position weight matrices (PWMs). With ever-increasing pool of TF binding motifs from multiple sources, redundancy issues are difficult to avoid, especially when every source maintains its own database for collection. One solution can be to cluster biologically relevant or similar PWMs, whether coming from experimental detection or in silico predictions. However, there is a lack of efficient tools to cluster PWMs. Assessing quality of PWM clusters is yet another challenge. Therefore, new methods and tools are required to efficiently cluster PWMs and assess quality of clusters. Results A new Python package Affinity Based Clustering for Position Weight Matrices (abc4pwm) was developed. It efficiently clustered PWMs from multiple sources with or without using DNA-Binding Domain (DBD) information, generated a representative motif for each cluster, evaluated the clustering quality automatically, and filtered out incorrectly clustered PWMs. Additionally, it was able to update human DBD family database automatically, classified known human TF PWMs to the respective DBD family, and performed TF motif searching and motif discovery by a new ensemble learning approach. Conclusion This work demonstrates applications of abc4pwm in the DNA sequence analysis for various high throughput sequencing data using ~ 1770 human TF PWMs. It recovered known TF motifs at gene promoters based on gene expression profiles (RNA-seq) and identified true TF binding targets for motifs predicted from ChIP-seq experiments. Abc4pwm is a useful tool for TF motif searching, clustering, quality assessment and integration in multiple types of sequence data analysis including RNA-seq, ChIP-seq and ATAC-seq.

• Liu, Xin; Risbakk, Sanne; Almeida Carvalho, Patricia; Yang, Mingyi; Backe, Paul Hoff & Bjørås, Magnar [Vis alle 8 forfattere av denne artikkelen] (2022). Immobilization of FeFe-hydrogenase on black TiO2 nanotubes as biocathodes for the hydrogen evolution reaction. Electrochemistry communications. ISSN 1388-2481. 135. doi: 10.1016/j.elecom.2022.107221. Fulltekst i vitenarkiv Vis sammendrag

  Hydrogenases are attractive biocatalysts for utilization in electrochemical devices as potential replacement for Pt in hydrogen evolving electrodes. In this work, we investigate the immobilization of ferredoxin tagged FeFe-hydrogenase (Fd-HydA1) on black TiO2 nanotubes (bTNTs), with uniform nanotube opening diameters of 140 nm. By utilizing an immunogold labelling method, we show that the enzymes attach on the top surface of the bTNTs film rather than on the inner nanotube walls, reflecting the difficulty to insert enzymes into high aspect-ratio nanomaterials for O2-shielding. Nevertheless, cyclic voltammetry demonstrates direct electron transfer between Fd-HydA1 and bTNTs for the hydrogen evolution reaction (HER) in neutral media. This work provides new insight towards design of new nanostructured electrodes for enzyme immobilization.

• Lobie, Tekle Airgecho; Roba, Aklilu Abrham; Booth, James; Kristiansen, Knut Ivan; Aseffa, Abraham & Skarstad, Kirsten Jane [Vis alle 7 forfattere av denne artikkelen] (2021). Antimicrobial resistance: A challenge awaiting the post-COVID-19 era. International Journal of Infectious Diseases. ISSN 1201-9712. 111, s. 322–325. doi: 10.1016/j.ijid.2021.09.003. Fulltekst i vitenarkiv
• Karlsen, Tom Rune; Kong, Xiang Yi; Holm, Sverre; Quiles-Jiménez, Ana M T; Dahl, Tuva Børresdatter & Yang, Kuan [Vis alle 17 forfattere av denne artikkelen] (2021). NEIL3-deficiency increases gut permeability and contributes to a pro-atherogenic metabolic phenotype. Scientific Reports. ISSN 2045-2322. 11(1). doi: 10.1038/s41598-021-98820-0. Fulltekst i vitenarkiv
• Meehyun, Ko; Chang, Soo Young; Byun, Soo Young; Ianevski, Aleksandr; Choi, Inhee & Pham Hung, Anne-Laure [Vis alle 13 forfattere av denne artikkelen] (2021). Screening of FDA-Approved Drugs Using a MERS-CoV Clinical Isolate from South Korea Identifies Potential Therapeutic Options for COVID-19. Viruses. ISSN 1999-4915. doi: 10.3390/v13040651. Fulltekst i vitenarkiv
• Kong, Xiang Yi; Huse, Camilla; Yang, Kuan; Øgaard, Jonas; Jimenez, Natalia Berges & Vik, Erik Sebastian [Vis alle 24 forfattere av denne artikkelen] (2021). Endonuclease v regulates atherosclerosis through c-c motif chemokine ligand 2-mediated monocyte infiltration. Journal of the American Heart Association (JAHA). ISSN 2047-9980. 10(14). doi: 10.1161/JAHA.120.020656. Fulltekst i vitenarkiv Vis sammendrag

  Background: In cardiovascular diseases, atherosclerotic disorder are the most frequent and important with respect to morbidity and mortality. Inflammation mediated by immune cells is central in all parts of the atherosclerotic progress, and further understanding of the underlying mechanisms is needed. Growing evidence suggests that deamination of adenosine‐to‐inosine in RNA is crucial for a correct immune response; nevertheless, the role of adenosine‐to‐inosine RNA editing in atherogenesis has barely been studied. Several proteins have affinity for inosines in RNA, one being ENDOV (endonuclease V), which binds and cleaves RNA at inosines. Data on ENDOV in atherosclerosis are lacking. Methods and Results: Quantitative polymerase chain reaction on ENDOV mRNA showed an increased level in human carotid atherosclerotic plaques compared with control veins. Inosine‐ribonuclease activity as measured by an enzyme activity assay is detected in immune cells relevant for the atherosclerotic process. Abolishing EndoV in atherogenic apolipoprotein E‐deficient (ApoE−/−) mice reduces the atherosclerotic plaque burden, both in size and lipid content. In addition, in a brain stroke model, mice without ENDOV suffer less damage than control mice. Finally, lack of EndoV reduces the recruitment of monocytes to atherosclerotic lesions in atherogenic ApoE−/− mice. Conclusions: ENDOV is upregulated in human atherosclerotic lesions, and data from mice suggest that ENDOV promotes atherogenesis by enhancing the monocyte recruitment into the atherosclerotic lesion, potentially by increasing the effect of CCL2 activation on these cells.

• Ianevski, Aleksandr; Yao, Rouan; Zusinaite, Eva; Lello, Laura Sandra; Wang, Sainan & Jo, Eunji [Vis alle 28 forfattere av denne artikkelen] (2021). Synergistic interferon-alpha-based combinations for treatment of sars-cov-2 and other viral infections. Viruses. ISSN 1999-4915. 13:2489(12), s. 1–18. doi: 10.3390/v13122489. Fulltekst i vitenarkiv Vis sammendrag

  Background: There is an urgent need for new antivirals with powerful therapeutic potential and tolerable side effects. Methods: Here, we tested the antiviral properties of interferons (IFNs), alone and with other drugs in vitro. Results: While IFNs alone were insufficient to completely abolish replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), IFNα, in combination with remdesivir, EIDD-2801, camostat, cycloheximide, or convalescent serum, proved to be more effective. Transcriptome and metabolomic analyses revealed that the IFNα–remdesivir combination suppressed SARS-CoV-2-mediated changes in Calu-3 cells and lung organoids, although it altered the homeostasis of uninfected cells and organoids. We also demonstrated that IFNα combinations with sofosbuvir, telaprevir, NITD008, ribavirin, pimodivir, or lamivudine were effective against HCV, HEV, FLuAV, or HIV at lower concentrations, compared to monotherapies. Conclusions: Altogether, our results indicated that IFNα can be combined with drugs that affect viral RNA transcription, protein synthesis, and processing to make synergistic combinations that can be attractive targets for further pre-clinical and clinical development against emerging and re-emerging viral infections.

• Sousa, Mirta; Ye, Jing; Gomez, Luisa Fernanda Luna; Hildrestrand, Gunn Annette; Bjørås, Karine & Scheffler, Katja [Vis alle 7 forfattere av denne artikkelen] (2021). Impact of oxidative DNA damage and the role of DNA glycosylases in neurological dysfunction. International Journal of Molecular Sciences. ISSN 1661-6596. 22:12924(23), s. 1–22. doi: 10.3390/ijms222312924. Fulltekst i vitenarkiv Vis sammendrag

  The human brain requires a high rate of oxygen consumption to perform intense metabolic activities, accounting for 20% of total body oxygen consumption. This high oxygen uptake results in the generation of free radicals, including reactive oxygen species (ROS), which, at physiological levels, are beneficial to the proper functioning of fundamental cellular processes. At supraphysiological levels, however, ROS and associated lesions cause detrimental effects in brain cells, commonly observed in several neurodegenerative disorders. In this review, we focus on the impact of oxidative DNA base lesions and the role of DNA glycosylase enzymes repairing these lesions on brain function and disease. Furthermore, we discuss the role of DNA base oxidation as an epigenetic mechanism involved in brain diseases, as well as potential roles of DNA glycosylases in different epigenetic contexts. We provide a detailed overview of the impact of DNA glycosylases on brain metabolism, cognition, inflammation, tissue loss and regeneration, and age-related neurodegenerative diseases based on evidence collected from animal and human models lacking these enzymes, as well as post-mortem studies on patients with neurological disorders.

• Steen, Kristian Wollen; Hagen, Lars; Vågbø, Cathrine Broberg; Rabe, Renana; Iveland, Tobias Solli & Aas, Per Arne [Vis alle 14 forfattere av denne artikkelen] (2021). ALKBH3 partner ASCC3 mediates P-body formation and selective clearance of MMS-induced 1-methyladenosine and 3-methylcytosine from mRNA. Journal of Translational Medicine. ISSN 1479-5876. 19(1). doi: 10.1186/s12967-021-02948-6. Fulltekst i vitenarkiv Vis sammendrag

  Background: Reversible enzymatic methylation of mammalian mRNA is widespread and serves crucial regulatory functions, but little is known to what degree chemical alkylators mediate overlapping modifcations and whether cells distinguish aberrant from canonical methylations. Methods: Here we use quantitative mass spectrometry to determine the fate of chemically induced methylbases in the mRNA of human cells. Concomitant alteration in the mRNA binding proteome was analyzed by SILAC mass spectrometry. Results: MMS induced prominent direct mRNA methylations that were chemically identical to endogenous methylbases. Transient loss of 40S ribosomal proteins from isolated mRNA suggests that aberrant methylbases mediate arrested translational initiation and potentially also no-go decay of the afected mRNA. Four proteins (ASCC3, YTHDC2, TRIM25 and GEMIN5) displayed increased mRNA binding after MMS treatment. ASCC3 is a binding partner of the DNA/RNA demethylase ALKBH3 and was recently shown to promote disassembly of collided ribosomes as part of the ribosome quality control (RQC) trigger complex. We fnd that ASCC3-defcient cells display delayed removal of MMSinduced 1-methyladenosine (m1A) and 3-methylcytosine (m3C) from mRNA and impaired formation of MMS-induced P-bodies. Conclusions: Our fndings conform to a model in which ASCC3-mediated disassembly of collided ribosomes allows demethylation of aberrant m1A and m3C by ALKBH3. Our fndings constitute frst evidence of selective sanitation of aberrant mRNA methylbases over their endogenous counterparts and warrant further studies on RNA-mediated efects of chemical alkylators commonly used in the clinic.

• Hildrestrand, Gunn Annette; Rolseth, Veslemøy; Kunath, Nicolas; Suganthan, Rajikala; Jensen, Vidar & Bugaj, Anna Maria [Vis alle 24 forfattere av denne artikkelen] (2021). NEIL1 and NEIL2 DNA glycosylases modulate anxiety and learning in a cooperative manner in mice. Communications Biology. ISSN 2399-3642. 4. doi: 10.1038/s42003-021-02864-x. Fulltekst i vitenarkiv
• Kunath, Nicolas; Bugaj, Anna Maria; Bigonah, Pegah; Fernandez Berrocal, Marion Silvana; Bjørås, Magnar & Ye, Jing (2021). DNA repair enzyme NEIL3 enables a stable neural representation of space by shaping transcription in hippocampal neurons. iScience. ISSN 2589-0042. 24(12). doi: 10.1016/j.isci.2021.103470. Fulltekst i vitenarkiv Vis sammendrag

  DNA repair enzymes are essential for the maintenance of the neuronal genome and thereby proper brain functions. Emerging evidence links DNA repair to epigenetic gene regulation; however, its contribution to different transcriptional programs required for neuronal functions remains elusive. In this study, we identified a role of the DNA repair enzyme NEIL3 in modulating the maturation and function of hippocampal CA1 neurons by shaping the CA1 transcriptome during postnatal development and in association with spatial behavior. We observed a delayed maturation in Neil3-/- CA1 and identified differentially regulated genes required for hippocampal development. We revealed impaired spatial stability in Neil3-/- CA1 place cells and found spatial experience-induced gene expression essential for synaptic plasticity. This is the first study that links molecular underpinnings of DNA repair to the neural basis of spatial cognition beyond animals' behavioral phenotypes, thus shedding light on the molecular determinants enabling a stable neural representation of space.

• Quiles-Jiménez, Ana M T; Gregersen, Ida; Segers, Filip; Skarpengland, Tonje; Kroustallaki, Pinelopi & Yang, Kuan [Vis alle 30 forfattere av denne artikkelen] (2021). DNA glycosylase Neil3 regulates vascular smooth muscle cell biology during atherosclerosis development. Atherosclerosis. ISSN 0021-9150. 324, s. 123–132. doi: 10.1016/j.atherosclerosis.2021.02.023. Fulltekst i vitenarkiv
• Ahmadi, Arash; Till, Katharina; Backe, Paul Hoff; Blicher, Pernille; Diekmann, Robin & Schüttpelz, Mark [Vis alle 10 forfattere av denne artikkelen] (2021). Non-flipping DNA glycosylase AlkD scans DNA without formation of a stable interrogation complex. Communications Biology. ISSN 2399-3642. 4. doi: 10.1038/s42003-021-02400-x. Fulltekst i vitenarkiv
• Maddirevula, Sateesh; Alameer, Seham; Ewida, Nour; Sousa, Mirta; Bjørås, Magnar & Vågbø, Cathrine Broberg [Vis alle 7 forfattere av denne artikkelen] (2021). Insight into ALKBH8-related intellectual developmental disability based on the first pathogenic missense variant. Human Genetics. ISSN 0340-6717. 141, s. 209–215. doi: 10.1007/s00439-021-02391-z. Vis sammendrag

  ALKBH8 is a methyltransferase that modifies tRNAs by methylating the anticodon wobble uridine residue. The syndrome of ALKBH8-related intellectual developmental disability (MRT71) has thus far been reported solely in the context of homozygous truncating variants that cluster in the last exon. This raises interesting questions about the disease mechanism, because these variants are predicted to escape nonsense mediated decay and yet they appear to be loss of function. Furthermore, the limited class of reported variants complicates the future interpretation of missense variants in ALKBH8. Here, we report a consanguineous family in which two children with MRT71-compatible phenotype are homozygous for a novel missense variant in the methyltransferase domain. We confirm the pathogenicity of this variant by demonstrating complete absence of ALKBH8-dependent modifications in patient cells. Targeted proteomics analysis of ALKBH8 indicates that the variant does not lead to loss of ALKBH8 protein expression. This report adds to the clinical delineation of MRT71, confirms loss of function of ALKBH8 as the disease mechanism and expands the repertoire of its molecular lesions.

• Ianevski, Aleksandr; Yao, Rouan; Lysvand, Hilde; Grødeland, Gunnveig; Legrand, Nicolas & Oksenych, Valentyn [Vis alle 10 forfattere av denne artikkelen] (2021). Nafamostat–interferon-α combination suppresses sars-cov-2 infection in vitro and in vivo by cooperatively targeting host tmprss2. Viruses. ISSN 1999-4915. 13:1768(9), s. 1–8. doi: 10.3390/v13091768. Fulltekst i vitenarkiv
• Ianevski, Aleksandr; Yao, Rouan; Zusinaite, Eva; Lysvand, Hilde; Oksenych, Valentyn & Tenson, Tanel [Vis alle 8 forfattere av denne artikkelen] (2021). Active components of commonly prescribed medicines affect influenza a virus–host cell interaction: A pilot study. Viruses. ISSN 1999-4915. 13(8), s. 1–14. doi: 10.3390/v13081537. Fulltekst i vitenarkiv
• Xu, Kaiqi; Chatzitakis, Athanasios Eleftherios; Backe, Paul Hoff; Ruan, Qiushi; Tang, Junwang & Rise, Frode [Vis alle 8 forfattere av denne artikkelen] (2021). In situ cofactor regeneration enables selective CO2 reduction in a stable and efficient enzymatic photoelectrochemical cell. Applied Catalysis B: Environmental. ISSN 0926-3373. 296. doi: 10.1016/j.apcatb.2021.120349. Fulltekst i vitenarkiv Vis sammendrag

  Mimicking natural photosynthesis by direct photoelectrochemical (PEC) reduction of CO2 to chemicals and fuels requires complex cell assemblies with limitations in selectivity, efficiency, cost, and stability. Here, we present a breakthrough cathode utilizing an oxygen tolerant formate dehydrogenase enzyme derived from clostridium carboxidivorans and coupled to a novel and efficient in situ nicotinamide adenine dinucleotide (NAD+/NADH) regeneration mechanism through interfacial electrochemistry on g-C3N4 films. We demonstrate stable (20 h) aerobic PEC CO2-to-formate reduction at close to 100 % faradaic efficiency and unit selectivity in a bio-hybrid PEC cell of minimal engineering with optimized Ta3N5 nanotube photoanode powered by simulated sunlight with a solar to fuel efficiency of 0.063 %, approaching that of natural photosynthesis.

• Aprigliano, Rossana; Aksu, Merdane Ezgi; Bradamante, Stefano; Mihaljevic, Boris; Wang, Wei & Rian, Kristin [Vis alle 19 forfattere av denne artikkelen] (2021). Increased p53 signaling impairs neural differentiation in HUWE1-promoted intellectual disabilities. Cell Reports Medicine. ISSN 2666-3791. 2(4). doi: 10.1016/j.xcrm.2021.100240. Fulltekst i vitenarkiv Vis sammendrag

  Essential E3 ubiquitin ligase HUWE1 (HECT, UBA, and WWE domain containing 1) regulates key factors, such as p53. Although mutations in HUWE1 cause heterogenous neurodevelopmental X-linked intellectual disabilities (XLIDs), the disease mechanisms common to these syndromes remain unknown. In this work, we identify p53 signaling as the central process altered in HUWE1-promoted XLID syndromes. By focusing on Juberg-Marsidi syndrome (JMS), one of the severest XLIDs, we show that increased p53 signaling results from p53 accumulation caused by HUWE1 p.G4310R destabilization. This further alters cell-cycle progression and proliferation in JMS cells. Modeling of JMS neurodevelopment reveals majorly impaired neural differentiation accompanied by increased p53 signaling. The neural differentiation defects can be successfully rescued by reducing p53 levels and restoring the expression of p53 target genes, in particular CDKN1A/p21. In summary, our findings suggest that increased p53 signaling underlies HUWE1-promoted syndromes and impairs XLID JMS neural differentiation.

• Ladner, Jason T.; Henson, Sierra N.; Boyle, Annalee S.; Engelbrektson, Anna L.; Fink, Zane W. & Rahee, Fatima [Vis alle 22 forfattere av denne artikkelen] (2021). Epitope-resolved profiling of the SARS-CoV-2 antibody response identifies cross-reactivity with endemic human coronaviruses. Cell Reports Medicine. ISSN 2666-3791. 2(1), s. 1–13. doi: 10.1016/j.xcrm.2020.100189. Fulltekst i vitenarkiv
• Quan, Zhilong; Luo, Chunyang; Zhu, Bitong; Zhao, Chungui; Yang, Mingyi & Bjørås, Magnar [Vis alle 8 forfattere av denne artikkelen] (2021). Synthesis and antimicrobial activities of chitosan/polypropylene carbonate-based nanoparticles. RSC Advances. ISSN 2046-2069. 11(17), s. 10121–10129. doi: 10.1039/d0ra09257f. Fulltekst i vitenarkiv
• Rolinski, Milosz; Montaldo, Nicola Pietro; Aksu, Merdane Ezgi; Martin, Sarah Fordyce; Brambilla, Alessandro & Kunath, Nicolas [Vis alle 13 forfattere av denne artikkelen] (2021). Loss of Mediator complex subunit 13 (MED13) promotes resistance to alkylation through cyclin D1 upregulation. Nucleic Acids Research (NAR). ISSN 0305-1048. 49(3), s. 1470–1484. doi: 10.1093/nar/gkaa1289. Fulltekst i vitenarkiv
• Lobie, Tekle Airgecho; Woldeamanuel, Yimtubezinash; Asrat, Daniel; Beyene, Demissew Balcha; Bjørås, Magnar & Aseffa, Abraham (2020). Genetic diversity and drug resistance pattern of Mycobacterium tuberculosis strains isolated from pulmonary tuberculosis patients in the Benishangul Gumuz region and its surroundings, Northwest Ethiopia. PLOS ONE. ISSN 1932-6203. 15(4). doi: 10.1371/journal.pone.0231320. Fulltekst i vitenarkiv
• Louwe, Maria Cornelia; Olsen, Maria Belland; Kaasbøll, Ole Jørgen; Yang, Kuan; Fosshaug, Linn Elisabeth Lillerud & Alfsnes, Katrine [Vis alle 25 forfattere av denne artikkelen] (2020). Absence of NLRP3 Inflammasome in Hematopoietic Cells Reduces Adverse Remodeling After Experimental Myocardial Infarction. JACC: Basic to Translational Science. ISSN 2452-302X. 5(12), s. 1210–1224. doi: 10.1016/j.jacbts.2020.09.013. Fulltekst i vitenarkiv
• Backe, Paul Hoff; Lærdahl, Jon Kristen; Kittelsen, Lene Svendsen; Dalhus, Bjørn; Mørkrid, Lars & Bjørås, Magnar (2020). Structural basis for substrate and product recognition in human phosphoglucomutase-1 (PGM1) isoform 2, a member of the α-D-phosphohexomutase superfamily. Scientific Reports. ISSN 2045-2322. 10(1). doi: 10.1038/s41598-020-62548-0. Fulltekst i vitenarkiv
• Ianevski, Aleksandr; Yao, Rouan; Biza, Svetlana; Zusinaite, Eva; Mannik, Andres & Kivi, Gaily [Vis alle 26 forfattere av denne artikkelen] (2020). Identification and Tracking of Antiviral Drug Combinations. Viruses. ISSN 1999-4915. 12(10). doi: 10.3390/v12101178. Fulltekst i vitenarkiv Vis sammendrag

  Combination therapies have become a standard for the treatment for HIV and hepatitis C virus (HCV) infections. They are advantageous over monotherapies due to better efficacy, reduced toxicity, as well as the ability to prevent the development of resistant viral strains and to treat viral co-infections. Here, we identify new synergistic combinations against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), echovirus 1 (EV1), hepatitis C virus (HCV) and human immunodeficiency virus 1 (HIV-1) in vitro. We observed synergistic activity of nelfinavir with convalescent serum and with purified neutralizing antibody 23G7 against SARS-CoV-2 in human lung epithelial Calu-3 cells. We also demonstrated synergistic activity of nelfinavir with EIDD-2801 or remdesivir in Calu-3 cells. In addition, we showed synergistic activity of vemurafenib with emetine, homoharringtonine, anisomycin, or cycloheximide against EV1 infection in human lung epithelial A549 cells. We also found that combinations of sofosbuvir with brequinar or niclosamide are synergistic against HCV infection in hepatocyte-derived Huh-7.5 cells, and that combinations of monensin with lamivudine or tenofovir are synergistic against HIV-1 infection in human cervical TZM-bl cells. These results indicate that synergy is achieved when a virus-directed antiviral is combined with another virus- or host-directed agent. Finally, we present an online resource that summarizes novel and known antiviral drug combinations and their developmental status.

• Booth, James; Špírek, Mário; Lobie, Tekle Airgecho; Skarstad, Kirsten Jane; Krejci, Lumir & Bjørås, Magnar (2020). Antibiotic-induced DNA damage results in a controlled loss of pH homeostasis and genome instability. Scientific Reports. ISSN 2045-2322. 10(1). doi: 10.1038/s41598-020-76426-2. Fulltekst i vitenarkiv
• Rodriguez-Vargas, José-Manuel; Martin-Hernandez, Kathline; Wang, Wei; Kunath, Nicolas; Suganthan, Rajikala & Amé, Jean-Christophe [Vis alle 10 forfattere av denne artikkelen] (2020). Parp3 promotes astrocytic differentiation through a tight regulation of Nox4-induced ROS and mTorc2 activation. Cell Death & Disease. ISSN 2041-4889. 11(11). doi: 10.1038/s41419-020-03167-5. Fulltekst i vitenarkiv
• Farooq, Amna; Grønmyr, Sindre; Omer, Ali Avan; Rognes, Torbjørn; Scheffler, Katja & Bjørås, Magnar [Vis alle 7 forfattere av denne artikkelen] (2020). HMST-Seq-Analyzer: A new python tool for differential methylation and hydroxymethylation analysis in various DNA methylation sequencing data. Computational and Structural Biotechnology Journal. ISSN 2001-0370. 18, s. 2877–2889. doi: 10.1016/j.csbj.2020.09.038. Fulltekst i vitenarkiv
• Kong, Xiang Yi; Vik, Erik Sebastian; Nawaz, Meh Sameen; Berges, Natalia; Dahl, Tuva Børresdatter & Vågbø, Cathrine [Vis alle 17 forfattere av denne artikkelen] (2020). Deletion of Endonuclease V suppresses chemically induced hepatocellular carcinoma. Nucleic Acids Research (NAR). ISSN 0305-1048. 48(8), s. 4463–4479. doi: 10.1093/nar/gkaa115. Fulltekst i vitenarkiv
• Polec, Anna; Rowe, Alexander D.; Blicher, Pernille; Suganthan, Rajikala; Bjørås, Magnar & Bøe, Stig Ove (2020). PML regulates the epidermal differentiation complex and skin morphogenesis during mouse embryogenesis. Genes. ISSN 2073-4425. 11(10). doi: 10.3390/genes11101130. Fulltekst i vitenarkiv
• Quiles Jimenez, Ana Maria Teresa; Gregersen, Ida; Sousa, Mirta; Abbas, Azhar; Kong, Xiang Yi & Alseth, Ingrun [Vis alle 13 forfattere av denne artikkelen] (2020). N6-methyladenosine in RNA of atherosclerotic plaques: An epitranscriptomic signature of human carotid atherosclerosis. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 533(4), s. 631–637. doi: 10.1016/j.bbrc.2020.09.057. Fulltekst i vitenarkiv
• Ianevski, Aleksandr; Kulesskiy, Evgeny; Krpina, Klara; Lou, Guofeng; Aman, Yahyah & Bugai, Andrii [Vis alle 27 forfattere av denne artikkelen] (2020). Chemical, Physical and Biological Triggers of Evolutionary Sonserved Bcl-xL-Mediated Apoptosis. Cancers. ISSN 2072-6694. 12(6), s. 1–19. doi: 10.3390/cancers12061694. Fulltekst i vitenarkiv
• Naeini, Alireza Sahaf; Farooq, Amna; Bjørås, Magnar & Wang, Junbai (2020). IGAP-integrative genome analysis pipeline reveals new gene regulatory model associated with nonspecific TF-DNA binding affinity. Computational and Structural Biotechnology Journal. ISSN 2001-0370. 18, s. 1270–1286. doi: 10.1016/j.csbj.2020.05.024.
• Yang, Mingyi; Lin, Xiaolin; Segers, Filip; Suganthan, Rajikala; Hildrestrand, Gunn Annette & Rinholm, Johanne Egge [Vis alle 21 forfattere av denne artikkelen] (2020). OXR1A, a Coactivator of PRMT5 Regulating Histone Arginine Methylation. Cell reports. ISSN 2211-1247. 30(12), s. 4165–4178. doi: 10.1016/j.celrep.2020.02.063. Fulltekst i vitenarkiv
• Ianevski, Aleksandr; Yao, Rouan; Fenstad, Mona H.; Biza, Svetlana; Zusinaite, Eva & Reisberg, Tuuli [Vis alle 20 forfattere av denne artikkelen] (2020). Potential Antiviral Options against SARS-CoV-2 Infection. Viruses. Viruses. ISSN 1999-4915. doi: 10.3390/v12060642. Fulltekst i vitenarkiv
• Nedal, Aina; Ræder, Synnøve Brandt; Dalhus, Bjørn; Helgesen, Emily; Forstrøm, Rune Johansen & Lindland, Kim [Vis alle 12 forfattere av denne artikkelen] (2020). Peptides containing the PCNA interacting motif APIM bind to the beta-clamp and inhibit bacterial growth and mutagenesis. Nucleic Acids Research (NAR). ISSN 0305-1048. 48(10), s. 5540–5554. doi: 10.1093/nar/gkaa278. Fulltekst i vitenarkiv
• Anderssen, Petter Inge; Ianevski, Aleksandr; Lysvand, Hilde; Vitkauskiene, Astra; Oksenych, Valentyn & Bjørås, Magnar [Vis alle 13 forfattere av denne artikkelen] (2020). Discovery and development of safe-in-man broad-spectrum antiviral agents. International Journal of Infectious Diseases. ISSN 1201-9712. 93, s. 268–276. doi: 10.1016/j.ijid.2020.02.018. Fulltekst i vitenarkiv
• Scheffler, Katja; Jalland, Clara Maria Osnes; Benestad, Sylvie L.; Moldal, Torfinn; Ersdal, Cecilie & Gunnes, Gjermund [Vis alle 9 forfattere av denne artikkelen] (2020). DNA glycosylase Neil2 contributes to genomic responses in the spleen during clinical prion disease. Free Radical Biology & Medicine. ISSN 0891-5849. 152, s. 348–354. doi: 10.1016/j.freeradbiomed.2020.03.030. Fulltekst i vitenarkiv Vis sammendrag

  The DNA glycosylase Neil2 is a member of the base excision repair (BER) family of enzymes, which are important for repair of oxidative DNA damage. Specifically, Neil2 participates in repair of oxidized bases in single-stranded DNA of transcriptionally active genes. Mice with genetic ablation of Neil2 (Neil2−/−) display no overt phenotypes, but an age-dependent accumulation of oxidative DNA damage and increased inflammatory responsiveness. In young mice intra-cerebrally inoculated with prions, vigorous prion propagation starts rapidly in the germinal follicles of the spleen due to inoculum spillover. Here, we compare experimental prion disease in Neil2−/− mice with that in wild-type mice at disease onset and end-stage. Specifically, we investigated disease progression, accumulation of DNA damage, and mitochondrial respiratory complex activity in brain and spleen. We used genome-wide RNA sequencing of the spleen to compare the immune responses to prion propagation between the two groups of mice, at both onset and end-stage prion disease. The Neil2−/− mice deteriorated more rapidly than wild-type mice after onset of clinical signs. Levels of DNA damage in brain increased in both mouse groups, slightly more in the Neil2−/− mice. Transcriptome data from spleen at disease onset were similar between the mouse groups with moderate genomic responses. However, at end-stage a substantial response was evident in the wild-type mice but not in Neil2−/− mice. Our data show that Neil2 counteracts toxic signaling in clinical prion disease, and this is separate from gross pathological manifestations and PrPSc accumulation.

• Xu, Kaiqi; Chatzitakis, Athanasios Eleftherios; Risbakk, Sanne; Yang, Mingyi; Backe, Paul Hoff & Grandcolas, Mathieu [Vis alle 8 forfattere av denne artikkelen] (2020). High performance and toxicity assessment of Ta3N5 nanotubes for photoelectrochemical water splitting. Catalysis Today. ISSN 0920-5861. doi: 10.1016/j.cattod.2019.12.031. Fulltekst i vitenarkiv Vis sammendrag

  In this work, Co-based cocatalysts are electrodeposited on mesoporous Ta3N5 nanotubes. The electrodeposition time is varied and the optimized photoelectrode reaches a photocurrent density of 6.3 mA/cm2 at 1.23 V vs. SHE, under simulated solar illumination of 1 Sun, in 1 M NaOH. The best performing electrode, apart from the high photocurrent density, shows improved stability under intense photoelectrochemical water splitting conditions. The dual function of the cocatalyst to improve not only the photoelectrochemical performance, but also the stability, is highlighted. Moreover, we adopted a simple protocol to assess the toxicity of Co and Ta contained nanostructured materials (representing used photoelectrodes) employing the human cell line HeLa S3 as target cells.

• Anderssen, Petter Inge; Krpina, Klara; Ianevski, Aleksandr; Shtaida, Nastassia; Jo, Eunji & Yang, Jaewon [Vis alle 15 forfattere av denne artikkelen] (2019). Novel antiviral activities of obatoclax, emetine, niclosamide, brequinar, and homoharringtonine. Viruses. ISSN 1999-4915. 11(10). doi: 10.3390/v11100964. Fulltekst i vitenarkiv
• Li, Xiang; Zhao, Qiongyi; Wei, Wei; Lin, Quan; Magnan, Christophe & Emami, Michael R [Vis alle 22 forfattere av denne artikkelen] (2019). The DNA modification N6-methyl-2 '-eoxyadenosine (m6dA) drives activity-induced gene expression and is required for fear extinction. Nature Neuroscience. ISSN 1097-6256. 22(4), s. 534–544. doi: 10.1038/s41593-019-0339-x.
• Montaldo, Nicola Pietro; Bordin, Diana Lilian; Brambilla, Alessandro; Rösinger, Marcel; Martin, Sarah Fordyce & Bjørås, Karine Øian [Vis alle 16 forfattere av denne artikkelen] (2019). Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression. Nature Communications. ISSN 2041-1723. 10(1). doi: 10.1038/s41467-019-13394-w. Fulltekst i vitenarkiv
• Zhang, Zhichao; Manaf, Adeel; Li, Yanjiao; Pérez, Sonia Peña; Suganthan, Rajikala & Dahl, John Arne [Vis alle 8 forfattere av denne artikkelen] (2019). Histone methylations define neural stem/progenitor cell subtypes in the mouse subventricular zone. Molecular Neurobiology. ISSN 0893-7648. doi: 10.1007/s12035-019-01777-5. Fulltekst i vitenarkiv
• Castaneda Zegarra, Sergio Miguel ; Huse, Camilla; Røsand, Øystein; Sarno, Antonio; Xing, Mengtan & Gago-Fuentes, Raquel [Vis alle 14 forfattere av denne artikkelen] (2019). Generation of a mouse model lacking the non-homologous end-joining factor Mri/Cyren. Biomolecules. ISSN 2218-273X. 9:798(12), s. 1–13. doi: 10.3390/biom9120798. Fulltekst i vitenarkiv Vis sammendrag

  Classical non-homologous end joining (NHEJ) is a molecular pathway that detects, processes, and ligates DNA double-strand breaks (DSBs) throughout the cell cycle. Mutations in several NHEJ genes result in neurological abnormalities and immunodeficiency both in humans and mice. The NHEJ pathway is required for V(D)J recombination in developing B and T lymphocytes, and for class switch recombination in mature B cells. The Ku heterodimer formed by Ku70 and Ku80 recognizes DSBs and facilitates the recruitment of accessory factors (e.g., DNA-PKcs, Artemis, Paxx and Mri/Cyren) and downstream core factor subunits X-ray repair cross-complementing group 4 (XRCC4), XRCC4-like factor (XLF), and DNA ligase 4 (Lig4). Accessory factors might be dispensable for the process, depending on the genetic background and DNA lesion type. To determine the physiological role of Mri in DNA repair and development, we introduced a frame-shift mutation in the Mri gene in mice. We then analyzed the development of Mri-deficient mice as well as wild type and immunodeficient controls. Mice lacking Mri possessed reduced levels of class switch recombination in B lymphocytes and slow proliferation of neuronal progenitors when compared to wild type littermates. Human cell lines lacking Mri were as sensitive to DSBs as the wild type controls. Overall, we concluded that Mri/Cyren is largely dispensable for DNA repair and mouse development.

• Ahmadi, Arash; Till, Katharina; Hafting, Yngve; Schuttpelz, Mark; Bjørås, Magnar & Glette, Kyrre [Vis alle 8 forfattere av denne artikkelen] (2019). Additive manufacturing of laminar flow cells for single-molecule experiments. Scientific Reports. ISSN 2045-2322. 9(1). doi: 10.1038/s41598-019-53151-z. Fulltekst i vitenarkiv
• Berges, Natalia; Nawaz, Meh Sameen; Dahl, Tuva Børresdatter; Hagen, Lars; Bjørås, Magnar & Lærdahl, Jon Kristen [Vis alle 7 forfattere av denne artikkelen] (2019). Complex alternative splicing of human Endonuclease V mRNA, but evidence for only a single protein isoform. PLOS ONE. ISSN 1932-6203. 14(11). doi: 10.1371/journal.pone.0225081. Fulltekst i vitenarkiv
• Bösl, Korbinian; Ianevski, Aleksandr; Than, Thoa T.; Anderssen, Petter Inge; Kuivanen, Suvi & Teppor, Mona [Vis alle 25 forfattere av denne artikkelen] (2019). Common nodes of virus-host interaction revealed through an integrated network analysis. Frontiers in Immunology. ISSN 1664-3224. 10. doi: 10.3389/fimmu.2019.02186. Fulltekst i vitenarkiv
• Scheffler, Katja; Bjørås, Karine Øian & Bjørås, Magnar (2019). Diverse functions of DNA glycosylases processing oxidative base lesions in brain. DNA Repair. ISSN 1568-7864. 81. doi: 10.1016/j.dnarep.2019.102665.
• Ystgaard, Martin Bogale; Scheffler, Katja; Suganthan, Rajikala; Bjørås, Magnar; Ranheim, Trine & Sagen, Ellen Lund [Vis alle 9 forfattere av denne artikkelen] (2019). Neuromodulatory effect of NLRP3 and ASC in neonatal hypoxic ischemic encephalopathy. Neonatology. ISSN 1661-7800. 115(4), s. 355–362. doi: 10.1159/000497200. Fulltekst i vitenarkiv Vis sammendrag

  Background: Following birth asphyxia there is a robust inflammatory response. NLRP3 is a receptor of the innate immune system. Upon activation, NLRP3 forms an inflammasome together with ASC and procaspase-1 to mediate release of IL-1β and IL-18. NLRP3 has previously been shown to be upregulated following neonatal hypoxic-ischemic (HI) brain injury in mice, but with no early effect on brain injury. Objective: We aimed to evaluate if deficiency of NLRP3 or ASC protects against neonatal HI brain damage 7 days after hypoxia-ischemia. Methods: C57BL/6J, NLRP3–/–, and ASC–/– mice were subjected to unilateral common carotid artery ligation followed by hypoxia at P9. Brain infarction, apoptosis, and microglial response were evaluated, as well as total RNA sequencing and examination of plasma levels of systemic proinflammatory cytokines. Results: NLRP3–/– mice showed significantly increased brain infarction volumes compared to wild-type (Wt) mice, while ASC–/– mice showed reduced brain infarction volumes after neonatal hypoxia-ischemia. The amount of activated microglia was increased in NLRP3–/– mice, while decreased in ASC–/– mice compared to Wt mice. Total RNA sequencing showed an impaired inflammatory transcriptional response in the hippocampus of NLRP3–/– mice. Plasma levels of IL-1β and IL-18 were not affected, but TNF was lower in NLRP3–/– and ASC–/– mice compared to Wt mice. Conclusion: ASC deficiency is neuroprotective in neonatal HI brain damage in mice, while NLRP3 deficiency increases brain damage.

• Ianevski, Aleksandr; Anderssen, Petter Inge; Merits, Andres; Bjørås, Magnar & Kainov, Denis (2019). Expanding the activity spectrum of antiviral agents. Drug Discovery Today. ISSN 1359-6446. 24(5), s. 1224–1228. doi: 10.1016/j.drudis.2019.04.006.
• Rognlien, Anne Gro Wesenberg; Wollen, Embjørg Julianne; Atneosen-Åsegg, Monica; Suganthan, Rajikala; Bjørås, Magnar & Saugstad, Ola Didrik (2019). Neonatal Ogg1/Mutyh knockout mice have altered inflammatory gene response compared to wildtype mice in the brain and lung after hypoxia-reoxygenation. Journal of Perinatal Medicine. ISSN 0300-5577. 47(1), s. 114–124. doi: 10.1515/jpm-2018-0172. Fulltekst i vitenarkiv
• Ahmadi, Arash; Rosnes, Ida; Blicher, Pernille; Diekmann, Robin; Schüttpelz, Mark & Glette, Kyrre [Vis alle 9 forfattere av denne artikkelen] (2018). Breaking the speed limit with multimode fast scanning of DNA by Endonuclease V. Nature Communications. ISSN 2041-1723. 9(1). doi: 10.1038/s41467-018-07797-4. Fulltekst i vitenarkiv
• Askeland, Georgina; Rodinova, Marie; Štufková, Hana; Dosoudilova, Zaneta; Baxa, Monika & Smatlikova, Petra [Vis alle 15 forfattere av denne artikkelen] (2018). A transgenic minipig model of Huntington's disease shows early signs of behavioral and molecular pathologies. Disease Models and Mechanisms. ISSN 1754-8403. 11(10). doi: 10.1242/dmm.035949. Fulltekst i vitenarkiv
• Zusinaite, Eva; Ianevski, Aleksandr; Niukkanen, Diana; Poranen, Minna M.; Bjørås, Magnar & Afset, Jan Egil [Vis alle 10 forfattere av denne artikkelen] (2018). A systems approach to study immuno- and neuro-modulatory properties of antiviral agents. Viruses. ISSN 1999-4915. 10(8). doi: 10.3390/v10080423. Fulltekst i vitenarkiv
• Askeland, Georgina; Dosoudilova, Zaneta; Rodinova, Marie; Klempir, Jiri; Liskova, Irena & Kusnierczyk, Anna [Vis alle 11 forfattere av denne artikkelen] (2018). Increased nuclear DNA damage precedes mitochondrial dysfunction in peripheral blood mononuclear cells from Huntington's disease patients. Scientific Reports. ISSN 2045-2322. 8(1). doi: 10.1038/s41598-018-27985-y. Fulltekst i vitenarkiv
• Reiten, Malin Rokseth; Malachin, Giulia; Kommisrud, Elisabeth; Østby, Gunn Charlotte; Waterhouse, Karin Elisabeth & Krogenæs, Anette Kristine [Vis alle 17 forfattere av denne artikkelen] (2018). Stress resilience of spermatozoa and blood mononuclear cells without prion protein. Frontiers in Molecular Biosciences. ISSN 2296-889X. 5:1, s. 1–15. doi: 10.3389/fmolb.2018.00001. Fulltekst i vitenarkiv
• Li, Miaomiao; Zhao, Xu; Wang, Wei; Shi, Hailing; Pan, Qingfei & Lu, Zhike [Vis alle 11 forfattere av denne artikkelen] (2018). Ythdf2-mediated m6A mRNA clearance modulates neural development in mice. Genome Biology. ISSN 1465-6906. 19(1). doi: 10.1186/s13059-018-1436-y. Fulltekst i vitenarkiv
• Alexeeva, Marina; Guragain, Prashanna; Tesfahun, Almaz Nigatu; Tomkuvienė, Miglė; Arshad, Aysha & Gerasimaitė, Rūta [Vis alle 13 forfattere av denne artikkelen] (2018). Excision of the doubly methylated base N4,5-dimethylcytosine from DNA by Escherichia coli Nei and Fpg proteins . Philosophical Transactions of the Royal Society of London. Biological Sciences. ISSN 0962-8436. 373(1748). doi: 10.1098/rstb.2017.0337. Fulltekst i vitenarkiv
• Ianevski, Aleksandr; Zusinaite, Eva; Kuivanen, Suvi; Strand, Mårten; Lysvand, Hilde & Teppor, Mona [Vis alle 34 forfattere av denne artikkelen] (2018). Novel activities of safe-in-human broad-spectrum antiviral agents. Antiviral Research. ISSN 0166-3542. 154, s. 174–182. doi: 10.1016/j.antiviral.2018.04.016.
• Scheffler, Katja; Rachek, Lyudmila; You, Panpan; Rowe, Alexander D.; Wang, Wei & Kuśnierczyk, Anna [Vis alle 9 forfattere av denne artikkelen] (2018). 8-oxoguanine DNA glycosylase (Ogg1) controls hepatic gluconeogenesis. DNA Repair. ISSN 1568-7864. 61, s. 56–62. doi: 10.1016/j.dnarep.2017.11.008. Fulltekst i vitenarkiv

Se alle arbeider i Cristin

• Bali, Nesrine; Brennhaug, Svein Jonsson; Bjørås, Magnar; Manaf, Adeel & Bandyopadhyay, Sulalit (2023). Design of Polymeric Magnetic Nanoparticles for Biomedical Applications.
• Ravlo, Erlend; Ianevski, Aleksandr; Starheim, Eirin Johannessen; Wang, Wei; Ji, Ping & Lysvand, Hilde [Vis alle 23 forfattere av denne artikkelen] (2023). Boosted production of antibodies that neutralized different SARS-CoV-2 variants in a COVID-19 convalescent following messenger RNA vaccination - a case study. International Journal of Infectious Diseases. ISSN 1201-9712. 137(Dec), s. 75–78. doi: 10.1016/j.ijid.2023.10.011. Fulltekst i vitenarkiv
• Schipmann, Stephanie; Sivakanesan, Sarankan; Rath, Daniel Stavne; Brandal, Petter; Vik-Mo, Einar Osland & Bjørås, Magnar [Vis alle 13 forfattere av denne artikkelen] (2023). Glioblastom hos voksne. Tidsskrift for Den norske legeforening. ISSN 0029-2001. 143(2), s. 151–154. doi: 10.4045/tidsskr.22.0314. Fulltekst i vitenarkiv
• Quiles-Jiménez, Ana M T; Sousa, Mirta; Huse, Camilla; Dyrhol-Riise, Anne Ma; Holter, Jan Cato & Christensen, Erik Egeland [Vis alle 12 forfattere av denne artikkelen] (2023). Severely ill COVID-19 patients have altered circulating levels of proteins controlling the epitranscriptome. Journal of Infection. ISSN 0163-4453. doi: 10.1016/j.jinf.2023.03.002.
• Bali, Nesrine; Manaf, Adeel; Brennhaug, Svein Jonsson; Bjørås, Magnar & Bandyopadhyay, Sulalit (2022). Optimization of Carboxyl-Coated Magnetic Nanoparticles for DNA Size Selection.
• Ali, Zeeshan; Ravlo, Erlend; Aas, Per Arne; Hagen, Lars; Erlandsen, Sten Even & Ottesen, Vegar [Vis alle 9 forfattere av denne artikkelen] (2022). Scalable Production of Silica-coated Magnetic Nanobeads and their Applications in Viral Diagnostics.
• Bjørås, Magnar (2020). Nobelpris for oppdagelse av hvordan celler tilpasser seg skiftende oksygennivå. Tidsskrift for Den norske legeforening. ISSN 0029-2001. 140(1), s. 1–2. doi: 10.4045/tidsskr.19.0764.
• Bandyopadhyay, Sulalit; Bjørås, Magnar & Steigedal, Tonje S. (2020). COVID-19 Diagnostic: From Small Scale to Production in a Few Weeks. [Tidsskrift]. NBS-nytt 2-2020.
• Bandyopadhyay, Sulalit; Bjørås, Magnar & Steigedal, Tonje S. (2020). COVID-19 Diagnostic: From Small Scale to Production in a Few Weeks. [Tidsskrift]. NKS Kjemi.
• Xu, Kaiqi; Chatzitakis, Athanasios Eleftherios; Backe, Paul Hoff; Ruan, Qiushi; Tang, Junwang & Bjørås, Magnar [Vis alle 8 forfattere av denne artikkelen] (2020). The use of NMR to assess the activity of formate dehydrogenase biocatalysts for CO2 utilization.
• Bösl, Korbinian; Ianevski, Aleksandr; Than, Thoa T.; Andersen, Petter I.; Kuivanen, Suvi & Mona, Teppor [Vis alle 25 forfattere av denne artikkelen] (2019). Critical Nodes of Virus–Host Interaction Revealed Through an Integrated Network Analysis.
• Ahmadi, Arash; Rosnes, Ida; Blicher, Pernille; Diekmann, Robin; Schuttpelz, Mark & Glette, Kyrre [Vis alle 9 forfattere av denne artikkelen] (2019). Publisher Correction: Breaking the speed limit with multimode fast scanning of DNA by Endonuclease V (Nature Communications, (2018), 9, 1, (5381), 10.1038/s41467-018-07797-4). Nature Communications. ISSN 2041-1723. 10(1). doi: 10.1038/s41467-019-10070-x.
• Bösl, Korbinian; Ianevski, Aleksandr; Than, Thoa T.; Andersen, Petter I.; Teppor, Mona & Zusinaite, Eva [Vis alle 23 forfattere av denne artikkelen] (2019). Critical Nodes of Viral Modulation Revealed Through an Integrated Network Analysis of Host-Virus Interaction Landscape.
• Bosshard, Matthias; Aprigliano, Rossana; Gattiker, Cristina; Palibrk, Vuk; Markkanen, Enni & Backe, Paul Hoff [Vis alle 13 forfattere av denne artikkelen] (2018). Publisher Correction: Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability. Scientific Reports. ISSN 2045-2322. 8(1). doi: 10.1038/s41598-018-24189-2.
• Liu, Xin; Chatzitakis, Athanasios Eleftherios; Almeida Carvalho, Patricia; Backe, Paul Hoff; Yang, Mingyi & Bjørås, Magnar [Vis alle 7 forfattere av denne artikkelen] (2018). Enzyme-assisted Catalysis on Black Titania Electrodes.

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