Arne Klungland

Professor - CRESCO - Senter for embryologi

English version of this page

E-post arne.klungland@medisin.uio.no

Mobiltelefon +47 47840305 47840305

Rom E479

Brukernavn

Besøksadresse SINTEF Forskningsveien 1

Postadresse Oslo Universitetssykehus, Rikshospitalet Forskningsveien 1, SINTEF-bygget 0373 Oslo

Last ned visittkort

Faglige interesser

Epigenetikk, meiose, embryoutvikling, RNA biologi, histonmodifikasjoner, DNA reparasjon.

Se også forskningsgruppens websider ved OUS.

Bakgrunn

2023 - d.d. Professor og senterleder, CRESCO, Senter for Embryologi

2021-2023 Instituttleder, Institutt for Biovitenskap, Universitetet i oslo

2016 - 2021 Seksjonsleder ved Forskningsseksjonen, Avdeling for mikrobiologi, Klinikk for laboratoriemedisin, Oslo Universitetssykehus

2013 - d.d. Professor II ved Seksjon for biokjemi, Avdeling for molekylærmedisin, Institutt for medisinske basalfag

2001 - 2016 Forsker ved Forskningsseksjonen, Avdeling for mikrobiologi, Klinikk for laboratoriemedisin, Oslo Universitetssykehus

2005 - 2013 Professor I ved Seksjon for biokjemi, Avdeling for molekylærmedisin, Institutt for medisinske basalfag

Verv

2012 - d.d. Medlem av Fagrådet for komparativ medisin, Oslo Universitetssykehus

2018 - d.d. Leder av Fagrådet for kjernefasiliteter, Oslo Universitetssykehus

Emneord: epigenetikk, embryoutvikling, RNA, histonmodifikasjoner, DNA reparasjon

Monshaugen, Ida; Luna, Luisa; Rhodes, Jayden; Kristiansen, Felicia Iselin Svensson; Lång, Anna Ulrika & Bøe, Stig Ove [Vis alle 10 forfattere av denne artikkelen] (2023). Depletion of the m1A writer TRMT6/TRMT61A reduces proliferation and resistance against cellular stress in bladder cancer. Frontiers in Oncology. ISSN 2234-943X. 13, s. 1–14. doi: 10.3389/fonc.2023.1334112. Fulltekst i vitenarkiv
Li, Yanjiao; Wang, Yunhao; Vera-Rodriguez, Maria; Lindeman, Leif Christopher; Skuggen, Linda Ellevog & Rasmussen, Erik Magne Koscielniak [Vis alle 21 forfattere av denne artikkelen] (2023). Single-cell m6A mapping in vivo using picoMeRIP–seq. Nature Biotechnology. ISSN 1087-0156. doi: 10.1038/s41587-023-01831-7. Fulltekst i vitenarkiv
Wang, Yunhao; Li, Yanjiao; Skuland, Trine; Zhou, Chengjie; Li, Aifu & Hashim, Adnan [Vis alle 13 forfattere av denne artikkelen] (2023). The RNA m<sup>6</sup>A landscape of mouse oocytes and preimplantation embryos. Nature Structural & Molecular Biology. ISSN 1545-9993. 30(5), s. 703–709. doi: 10.1038/s41594-023-00969-x.
Sui, Xuesong; Klungland, Arne & Gao, Lu (2022). RNA m6A modifications in mammalian gametogenesis and pregnancy. Reproduction. ISSN 1470-1626. 165(1), s. R1–R8. doi: 10.1530/REP-22-0112.
Su, Zhangli; Monshaugen, Ida; Klungland, Arne; Ougland, Rune & Dutta, Anindya (2022). Characterization of novel small non-coding RNAs and their modifications in bladder cancer using an updated small RNA-seq workflow. Frontiers in Molecular Biosciences. ISSN 2296-889X. 9. doi: 10.3389/fmolb.2022.887686. Fulltekst i vitenarkiv
Lång, Emma Helena; Pedersen, Christian; Lång, Anna Ulrika; Blicher, Pernille; Klungland, Arne & Carlson, Andreas [Vis alle 7 forfattere av denne artikkelen] (2022). Mechanical coupling of supracellular stress amplification and tissue fluidization during exit from quiescence. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 119(32). doi: 10.1073/pnas.2201328119. Fulltekst i vitenarkiv
Khodeer, Sherif; Klungland, Arne & Dahl, John Arne (2022). ALKBH5 regulates somatic cell reprogramming in a phase-specific manner. Journal of Cell Science. ISSN 0021-9533. 135(11). doi: 10.1242/jcs.259824. Fulltekst i vitenarkiv
Su, Zhangli; Monshaugen, Ida; Wilson, Briana; Wang, Fengbin; Klungland, Arne & Ougland, Rune [Vis alle 7 forfattere av denne artikkelen] (2022). TRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancer. Nature Communications. ISSN 2041-1723. 13(1). doi: 10.1038/s41467-022-29790-8. Fulltekst i vitenarkiv
Zhao, Xu; Hendriks, Ivo A; Le Gras, Stephanie; Ye, Tao; Ramos Alonso, Lucia & Nguéa P, Aurélie [Vis alle 13 forfattere av denne artikkelen] (2022). Waves of sumoylation support transcription dynamics during adipocyte differentiation. Nucleic Acids Research (NAR). ISSN 0305-1048. 50(3), s. 1351–1369. doi: 10.1093/nar/gkac027. Fulltekst i vitenarkiv
Alexeeva, Marina; Moen, Marivi Nabong; Xu, Xiang Ming; Rasmussen, Anette; Leiros, Ingar & Kirpekar, Finn [Vis alle 10 forfattere av denne artikkelen] (2021). Intrinsic Strand-Incision Activity of Human UNG: Implications for Nick Generation in Immunoglobulin Gene Diversification. Frontiers in Immunology. ISSN 1664-3224. 12. doi: 10.3389/fimmu.2021.762032. Fulltekst i vitenarkiv
Zhang, Li-Sheng; Xiong, Qing-Ping; Pérez, Sonia Peña; Liu, Chang; Wei, Jiangbo & Le, Cassy [Vis alle 19 forfattere av denne artikkelen] (2021). ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing. Nature Cell Biology. ISSN 1465-7392. 23(7), s. 684–691. doi: 10.1038/s41556-021-00709-7.
Jin, Kang-Xuan; Zuo, Rujuan; Anastassiadis, Konstantinos; Klungland, Arne; Marr, Carsten & Filipczyk, Adam (2021). N6-methyladenosine (m6A) depletion regulates pluripotency exit by activating signaling pathways in embryonic stem cells. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 118(51). doi: 10.1073/pnas.2105192118. Fulltekst i vitenarkiv
Hildrestrand, Gunn Annette; Rolseth, Veslemøy; Kunath, Nicolas; Suganthan, Rajikala; Jensen, Vidar & Bugaj, Anna Maria [Vis alle 24 forfattere av denne artikkelen] (2021). NEIL1 and NEIL2 DNA glycosylases modulate anxiety and learning in a cooperative manner in mice. Communications Biology. ISSN 2399-3642. 4. doi: 10.1038/s42003-021-02864-x. Fulltekst i vitenarkiv
Tesfahun, Almaz Nigatu; Alexeeva, Marina; Tomkuvienė, Miglė; Arshad, Aysha; Guragain, Prashanna & Klungland, Arne [Vis alle 9 forfattere av denne artikkelen] (2021). Alleviation of C⋅C Mismatches in DNA by the Escherichia coli Fpg Protein. Frontiers in Microbiology. ISSN 1664-302X. 12. doi: 10.3389/fmicb.2021.608839. Fulltekst i vitenarkiv
Sankar, Aditya; Lerdrup, Mads; Manaf, Adeel; Johansen, Jens Vilstrup; Gonzalez, Javier Martin & Borup, Rehannah [Vis alle 13 forfattere av denne artikkelen] (2020). KDM4A regulates the maternal-to-zygotic transition by protecting broad H3K4me3 domains from H3K9me3 invasion in oocytes. Nature Cell Biology. ISSN 1465-7392. 22(4), s. 380–388. doi: 10.1038/s41556-020-0494-z.
Kong, Xiang Yi; Vik, Erik Sebastian; Nawaz, Meh Sameen; Berges, Natalia; Dahl, Tuva Børresdatter & Vågbø, Cathrine [Vis alle 17 forfattere av denne artikkelen] (2020). Deletion of Endonuclease V suppresses chemically induced hepatocellular carcinoma. Nucleic Acids Research (NAR). ISSN 0305-1048. 48(8), s. 4463–4479. doi: 10.1093/nar/gkaa115. Fulltekst i vitenarkiv
Li, Miaomiao & Klungland, Arne (2020). Modifications and interactions at the R-loop. DNA Repair. ISSN 1568-7864. 96. doi: 10.1016/j.dnarep.2020.102958.
Yang, Mingyi; Lin, Xiaolin; Segers, Filip; Suganthan, Rajikala; Hildrestrand, Gunn Annette & Rinholm, Johanne Egge [Vis alle 21 forfattere av denne artikkelen] (2020). OXR1A, a Coactivator of PRMT5 Regulating Histone Arginine Methylation. Cell reports. ISSN 2211-1247. 30(12), s. 4165–4178. doi: 10.1016/j.celrep.2020.02.063. Fulltekst i vitenarkiv
Grøsvik, Kristin; Tesfahun, Almaz Nigatu; Muruzábal-Lecumberri, Izaskun; Haugland, Gyri Teien; Leiros, Ingar & Ruoff, Peter [Vis alle 15 forfattere av denne artikkelen] (2020). The Escherichia coli alkA Gene Is Activated to Alleviate Mutagenesis by an Oxidized Deoxynucleoside. Frontiers in Microbiology. ISSN 1664-302X. 11. doi: 10.3389/fmicb.2020.00263. Fulltekst i vitenarkiv
Pilžys, Tomaš; Marcinkowski, Michał; Kukwa, Wojciech; Garbicz, Damian; Dylewska, Małgorzata & Ferenc, Karolina [Vis alle 15 forfattere av denne artikkelen] (2019). ALKBH overexpression in head and neck cancer: potential target for novel anticancer therapy. Scientific Reports. ISSN 2045-2322. 9(1). doi: 10.1038/s41598-019-49550-x.
Abakir, Abdulkadir; Giles, Tom C.; Cristini, Agnese; Foster, Jeremy M.; Dai, Nan & Starczak, Marta [Vis alle 22 forfattere av denne artikkelen] (2019). N6-methyladenosine regulates the stability of RNA:DNA hybrids in human cells. Nature Genetics. ISSN 1061-4036. 52(1), s. 48–55. doi: 10.1038/s41588-019-0549-x.
Zhang, Zhichao; Manaf, Adeel; Li, Yanjiao; Pérez, Sonia Peña; Suganthan, Rajikala & Dahl, John Arne [Vis alle 8 forfattere av denne artikkelen] (2019). Histone methylations define neural stem/progenitor cell subtypes in the mouse subventricular zone. Molecular Neurobiology. ISSN 0893-7648. doi: 10.1007/s12035-019-01777-5. Fulltekst i vitenarkiv
Jarøy, Emilie Grimnes; Acosta Jiménez, Lourdes Patricia; Hotta, Ryo; Goldstein, Allan M.; Emblem, Ragnhild & Klungland, Arne [Vis alle 7 forfattere av denne artikkelen] (2019). "Too much guts and not enough brains": (epi)genetic mechanisms and future therapies of Hirschsprung disease - a review. Clinical Epigenetics. ISSN 1868-7075. 11. doi: 10.1186/s13148-019-0718-x. Fulltekst i vitenarkiv
Prikrylova, Terezia; Robertson, Julia; Ferrucci, Francesca; Konorska, Dorota Joanna; Aanes, Håvard & Manaf, Adeel [Vis alle 16 forfattere av denne artikkelen] (2019). 5-hydroxymethylcytosine marks mammalian origins acting as a barrier to replication. Scientific Reports. ISSN 2045-2322. 9. doi: 10.1038/s41598-019-47528-3. Fulltekst i vitenarkiv
Alexeeva, Marina; Moen, Marivi Nabong; Grøsvik, Kristin; Tesfahun, Almaz Nigatu; Xu, Xiang Ming & Muruzábal-Lecumberri, Izaskun [Vis alle 12 forfattere av denne artikkelen] (2018). Excision of uracil from DNA by hSMUG1 includes strand incision and processing. Nucleic Acids Research (NAR). ISSN 0305-1048. 47(2), s. 779–793. doi: 10.1093/nar/gky1184. Fulltekst i vitenarkiv
Yaddehi Gamage, Thilini Hansamali Gamage; Gunnes, Gjermund; Lee, Robert Hugh; Louch, William Edward; Holmgren, Asbjørn & Bruton, Joseph D. [Vis alle 19 forfattere av denne artikkelen] (2018). STIM1 R304W causes muscle degeneration and impaired platelet activation in mice. Cell Calcium. ISSN 0143-4160. 76, s. 87–100. doi: 10.1016/j.ceca.2018.10.001. Fulltekst i vitenarkiv Vis sammendrag
STIM1 and ORAI1 regulate store-operated Ca2+ entry (SOCE) in most cell types, and mutations in these proteins have deleterious and diverse effects. We established a mouse line expressing the STIM1 R304 W gain-of-function mutation causing Stormorken syndrome to explore effects on organ and cell physiology. While STIM1 R304 W was lethal in the homozygous state, surviving mice presented with reduced growth, skeletal muscle degeneration, and reduced exercise endurance. Variable STIM1 expression levels between tissues directly impacted cellular SOCE capacity. In contrast to patients with Stormorken syndrome, STIM1 was downregulated in fibroblasts from Stim1R304W/R304W mice, which maintained SOCE despite constitutive protein activity. In studies using foetal liver chimeras, STIM1 protein was undetectable in homozygous megakaryocytes and platelets, resulting in impaired platelet activation and absent SOCE. These data indicate that downregulation of STIM1 R304 W effectively opposes the gain-of-function phenotype associated with this mutation, and highlight the importance of STIM1 in skeletal muscle development and integrity.
Wei, Jiangbo; Liu, Fange; Lu, Zhike; Fei, Qili; Ai, Yuxi & He, P. Cody [Vis alle 13 forfattere av denne artikkelen] (2018). Differential m6A, m6Am, and m1A Demethylation Mediated by FTO in the Cell Nucleus and Cytoplasm. Molecular Cell. ISSN 1097-2765. 71(6), s. 973–985. doi: 10.1016/j.molcel.2018.08.011.
Askeland, Georgina; Rodinova, Marie; Štufková, Hana; Dosoudilova, Zaneta; Baxa, Monika & Smatlikova, Petra [Vis alle 15 forfattere av denne artikkelen] (2018). A transgenic minipig model of Huntington's disease shows early signs of behavioral and molecular pathologies. Disease Models and Mechanisms. ISSN 1754-8403. 11(10). doi: 10.1242/dmm.035949. Fulltekst i vitenarkiv
Askeland, Georgina; Dosoudilova, Zaneta; Rodinova, Marie; Klempir, Jiri; Liskova, Irena & Kusnierczyk, Anna [Vis alle 11 forfattere av denne artikkelen] (2018). Increased nuclear DNA damage precedes mitochondrial dysfunction in peripheral blood mononuclear cells from Huntington's disease patients. Scientific Reports. ISSN 2045-2322. 8(1). doi: 10.1038/s41598-018-27985-y. Fulltekst i vitenarkiv
Ma, Chunhui; Chang, Mengqi; Lv, Hongyi; Zhang, Zhi-Wei; Zhang, Weilong & He, Xue [Vis alle 17 forfattere av denne artikkelen] (2018). RNA m6A methylation participates in regulation of postnatal development of the mouse cerebellum. Genome Biology. ISSN 1465-6906. 19(1). doi: 10.1186/s13059-018-1435-z. Fulltekst i vitenarkiv
Li, Miaomiao; Zhao, Xu; Wang, Wei; Shi, Hailing; Pan, Qingfei & Lu, Zhike [Vis alle 11 forfattere av denne artikkelen] (2018). Ythdf2-mediated m6A mRNA clearance modulates neural development in mice. Genome Biology. ISSN 1465-6906. 19(1). doi: 10.1186/s13059-018-1436-y. Fulltekst i vitenarkiv
Alexeeva, Marina; Guragain, Prashanna; Tesfahun, Almaz Nigatu; Tomkuvienė, Miglė; Arshad, Aysha & Gerasimaitė, Rūta [Vis alle 13 forfattere av denne artikkelen] (2018). Excision of the doubly methylated base N4,5-dimethylcytosine from DNA by Escherichia coli Nei and Fpg proteins . Philosophical Transactions of the Royal Society of London. Biological Sciences. ISSN 0962-8436. 373(1748). doi: 10.1098/rstb.2017.0337. Fulltekst i vitenarkiv
Jordan, Jennifer J.; Chhim, Sophea; Margulies, Carrie M.; Allocca, Mariacarmela; Bronson, Roderick T. & Klungland, Arne [Vis alle 8 forfattere av denne artikkelen] (2017). ALKBH7 drives a tissue and sex-specific necrotic cell death response following alkylation-induced damage. Cell Death & Disease. ISSN 2041-4889. 8. doi: 10.1038/cddis.2017.343.
Tang, Chong; Klukovich, Rachel; Peng, Hongying; Wang, Zhuqing; Yu, Tian & Zhang, Ying [Vis alle 9 forfattere av denne artikkelen] (2017). ALKBH5-dependent m6A demethylation controls splicing and stability of long 3'-UTR mRNAs in male germ cells. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 115(2), s. E325–E333. doi: 10.1073/pnas.1717794115.
Pawar, Tina; Bjørås, Magnar; Klungland, Arne & Eide, Lars (2017). Metabolism and DNA repair shape a specific modification pattern in mitochondrial DNA. Mitochondrion (Amsterdam. Print). ISSN 1567-7249. 40, s. 16–28. doi: 10.1016/j.mito.2017.09.002. Fulltekst i vitenarkiv
Landfors, Miriam; Johansen, Jostein; Aronsen, Jan Magnus; Vågbø, Cathrine Broberg; Doré, Louis C. & He, Chuan [Vis alle 13 forfattere av denne artikkelen] (2017). Genome-wide profiling of DNA 5-hydroxymethylcytosine during rat Sertoli cell maturation. Cell Discovery. ISSN 2056-5968. 3. doi: 10.1038/celldisc.2017.13. Fulltekst i vitenarkiv
Seifermann, Marco; Ulges, Alexander; Bopp, Tobias; Melcea, Svetlana; Schäfer, Andrea & Oka, Sugako [Vis alle 10 forfattere av denne artikkelen] (2017). Role of the DNA repair glycosylase OGG1 in the activation of murine splenocytes. DNA Repair. ISSN 1568-7864. 58, s. 13–20. doi: 10.1016/j.dnarep.2017.08.005.
Aas, Aleksander; Isakson, Pauline; Bindesbøll, Christian; Alemu, Endalkachew Ashenafi; Klungland, Arne & Simonsen, Anne (2017). Nucleocytoplasmic shuttling of FTO does not affect starvation-induced autophagy. PLOS ONE. ISSN 1932-6203. 12(3). doi: 10.1371/journal.pone.0168182. Fulltekst i vitenarkiv
Kalikstad, Betty; Kultima, Hanna Goransson; Andersstuen, Terese; Klungland, Arne & Isaksson, Anders (2017). Gene expression profiles in preterm infants on continuous long-term oxygen therapy suggest reduced oxidative stress-dependent signaling during hypoxia. Molecular Medicine Reports. ISSN 1791-2997. 15(4), s. 1513–1526. doi: 10.3892/mmr.2017.6185. Fulltekst i vitenarkiv
Rolseth, Veslemøy; Gomez, Luisa Fernanda Luna; Olsen, Ann-Karin; Suganthan, Rajikala; Scheffler, Katja & Neurauter, Christine Gran [Vis alle 15 forfattere av denne artikkelen] (2017). No cancer predisposition or increased spontaneous mutation frequencies in NEIL DNA glycosylases-deficient mice. Scientific Reports. ISSN 2045-2322. 7. doi: 10.1038/s41598-017-04472-4. Fulltekst i vitenarkiv
Chymkowitch, Pierre; Nguéa P, Aurélie; Aanes, Håvard; Robertson, Joseph; Klungland, Arne & Enserink, Jorrit (2017). TORC1-dependent sumoylation of Rpc82 promotes RNA polymerase III assembly and activity. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 114(5), s. 1039–1044. doi: 10.1073/pnas.1615093114.
Olsen, Maria Belland; Hildrestrand, Gunn Annette; Scheffler, Katja; Vinge, Leif Erik; Alfsnes, Katrine & Palibrk, Vuk [Vis alle 29 forfattere av denne artikkelen] (2017). NEIL3-Dependent Regulation of Cardiac Fibroblast Proliferation Prevents Myocardial Rupture. Cell reports. ISSN 2211-1247. 18, s. 82–92. doi: 10.1016/j.celrep.2016.12.009. Fulltekst i vitenarkiv
Klungland, Arne & Robertson, Adam Brian (2016). Oxidized C5-methyl cytosine bases in DNA: 5-Hydroxymethylcytosine; 5-formylcytosine; and 5-carboxycytosine. Free Radical Biology & Medicine. ISSN 0891-5849. 107, s. 62–68. doi: 10.1016/j.freeradbiomed.2016.11.038.
Alemu, Endalkachew Ashenafi; He, Chuan & Klungland, Arne (2016). ALKBHs-facilitated RNA modifications and de-modifications. DNA Repair. ISSN 1568-7864. 44, s. 87–91. doi: 10.1016/j.dnarep.2016.05.026.
Klungland, Arne; Dahl, John Arne; Greggains, Gareth; Fedorcsák, Péter & Filipczyk, Adam (2016). Reversible RNA modifications in meiosis and pluripotency. Nature Methods. ISSN 1548-7091. 14(1), s. 18–22. doi: 10.1038/nmeth.4111.
Møllersen, Linda; Moldestad, Olve; Rowe, Alexander D.; Bjølgerud, Anja; Holm, Ingunn & Tveterås, Linda Augustin [Vis alle 8 forfattere av denne artikkelen] (2016). Effects of anthocyanins on CAG repeat instability and behaviour in Huntington's disease R6/1 mice. PLOS Currents. ISSN 2157-3999. 8(HuntingtonDisease, 2016). doi: 10.1371/currents.hd.58d04209ab6d5de0844db7ef5628ff67.
Tekpli, Xavier; Urbanucci, Alfonso; Hashim, Adnan; Vågbø, Cathrine Broberg; Lyle, Robert & Kringen, Marianne K. [Vis alle 11 forfattere av denne artikkelen] (2016). Changes of 5-hydroxymethylcytosine distribution during myeloid and lymphoid differentiation of CD34+ cells. Epigenetics & Chromatin. ISSN 1756-8935. 9. doi: 10.1186/s13072-016-0070-8. Fulltekst i vitenarkiv
Vindedal, Gry Fluge; Thoren, Anna; Jensen, Vidar; Klungland, Arne; Zhang, Yong & Holtzman, Michael [Vis alle 8 forfattere av denne artikkelen] (2016). Removal of aquaporin-4 from glial and ependymal membranes causes brain water accumulation. Molecular and Cellular Neuroscience. ISSN 1044-7431. 77, s. 47–52. doi: 10.1016/j.mcn.2016.10.004.
Dahl, John Arne; Jung, Inkyung; Aanes, Håvard; Greggains, Gareth; Manaf, Adeel & Lerdrup, Mads [Vis alle 20 forfattere av denne artikkelen] (2016). Broad histone H3K4me3 domains in mouse oocytes modulate maternal-to-zygotic transition. Nature. ISSN 0028-0836. 537(7621), s. 548–552. doi: 10.1038/nature19360.
Liu, Fange; Clark, Wesley; Luo, Guanzheng; Wang, Xiaoyun; Fu, Ye & Wei, Jiangbo [Vis alle 16 forfattere av denne artikkelen] (2016). ALKBH1-Mediated tRNA Demethylation Regulates Translation. Cell. ISSN 0092-8674. 167(3), s. 816–828. doi: 10.1016/j.cell.2016.09.038.
Lauritzen, Knut Husø; Hasan-Olive, Md Mahdi; Regnell, Christine E; Kleppa, Liv; Scheibye Knudsen, Morten & Gjedde, Albert [Vis alle 10 forfattere av denne artikkelen] (2016). A ketogenic diet accelerates neurodegeneration in mice with induced mitochondrial DNA toxicity in the forebrain. Neurobiology of Aging. ISSN 0197-4580. 48, s. 34–47. doi: 10.1016/j.neurobiolaging.2016.08.005.
Ougland, Rune; Jonson, Ida; Moen, Marivi Nabong; Nesse, Gaute; Asker, Gry & Klungland, Arne [Vis alle 7 forfattere av denne artikkelen] (2016). Role of ALKBH1 in the Core Transcriptional Network of Embryonic Stem Cells. Cellular Physiology and Biochemistry. ISSN 1015-8987. 38(1), s. 173–184. doi: 10.1159/000438619.
Landfors, Miriam; Nakken, Sigve; Fusser, Markus; Dahl, John Arne; Klungland, Arne & Fedorcsák, Péter (2016). Sequencing of FTO and ALKBH5 in men undergoing infertility work-up identifies an infertility-associated variant and two missense mutations. Fertility and Sterility. ISSN 0015-0282. 105(5), s. 1170–1179e5. doi: 10.1016/j.fertnstert.2016.01.002.
Weber, Alain R.; Krawczyk, Claudia; Robertson, Adam Brian; Kusnierczyk, Anna; Vågbø, Cathrine Broberg & Schürmann, David [Vis alle 8 forfattere av denne artikkelen] (2016). Biochemical reconstitution of TET1-TDG-BER-dependent active DNA demethylation reveals a highly coordinated mechanism. Nature Communications. ISSN 2041-1723. 7. doi: 10.1038/ncomms10806. Fulltekst i vitenarkiv
Mi, Shuangli; Klungland, Arne & Yang, Yun-Gui (2016). Base-excision repair and beyond —A short summary attributed to scientific achievements of Tomas Lindahl, Nobel Prize Laureate in Chemistry 2015. Science China Life Sciences. ISSN 1674-7305. 59(1), s. 89–92. doi: 10.1007/s11427-015-4983-4.
Rasmussen, Erik; Vågbø, Cathrine Broberg; Münch, Daniel; Krokan, Hans Einar; Klungland, Arne & Amdam, Gro Vang [Vis alle 7 forfattere av denne artikkelen] (2016). DNA base modifications in honey bee and fruit fly genomes suggest an active demethylation machinery with species- and tissue-specific turnover rates. Biochemistry and Biophysics Reports. ISSN 2405-5808. 6, s. 9–15. doi: 10.1016/j.bbrep.2016.02.011. Fulltekst i vitenarkiv
Ougland, Rune; Rognes, Torbjørn; Klungland, Arne & Larsen, Elisabeth (2015). Non-homologous functions of the AlkB homologs. Journal of Molecular Cell Biology. ISSN 1674-2788. 7(6), s. 494–504. doi: 10.1093/jmcb/mjv029.
Bjørge, Monica Dahl; Hildrestrand, Gunn Annette; Scheffler, Katja; Suganthan, Rajikala; Rolseth, Veslemøy & Kusnierczyk, Anna [Vis alle 15 forfattere av denne artikkelen] (2015). Synergistic Actions of Ogg1 and Mutyh DNA Glycosylases Modulate Anxiety-like Behavior in Mice. Cell reports. ISSN 2211-1247. 13(12), s. 2671–2678. doi: 10.1016/j.celrep.2015.12.001. Fulltekst i vitenarkiv Vis sammendrag
Ogg1 and Mutyh DNA glycosylases cooperate to prevent mutations caused by 8-oxoG, a major premutagenic DNA lesion associated with cognitive decline. We have examined behavior and cognitive function in mice deficient of these glycosylases. Ogg1−/−Mutyh−/− mice were more active and less anxious, with impaired learning ability. In contrast, Mutyh−/− mice showed moderately improved memory. We observed no apparent change in genomic 8-oxoG levels, suggesting that Ogg1 and Mutyh play minor roles in global repair in adult brain. Notably, transcriptome analysis of hippocampus revealed that differentially expressed genes in the mutants belong to pathways known to be involved in anxiety and cognition. Esr1 targets were upregulated, suggesting a role of Ogg1 and Mutyh in repression of Esr1 signaling. Thus, beyond their involvement in DNA repair, Ogg1 and Mutyh regulate hippocampal gene expression related to cognition and behavior, suggesting a role for the glycosylases in regulating adaptive behavior.
Fusser, Markus; Kernstock, Stefan; Aileni, Vinay Kumar; Jacobsen, Wolfgang Mathias; Falnes, Pål & Klungland, Arne (2015). Lysine methylation of the valosin-containing protein (VCP) is dispensable for development and survival of mice. PLOS ONE. ISSN 1932-6203. 10(11). doi: 10.1371/journal.pone.0141472. Fulltekst i vitenarkiv
Chymkowitch, Pierre; Nguéa P, Aurélie; Aanes, Håvard; Koehler, Christian; Thiede, Bernd & Lorenz, Susanne [Vis alle 9 forfattere av denne artikkelen] (2015). Sumoylation of Rap1 mediates the recruitment of TFIID to promote transcription of ribosomal protein genes. Genome Research. ISSN 1088-9051. 25(6), s. 897–906. doi: 10.1101/gr.185793.114.
Ke, Shengdong; Alemu, Endalkachew Ashenafi; Mertens, Claudia; Gantman, Emily Conn; Fak, John J. & Mele, Aldo [Vis alle 15 forfattere av denne artikkelen] (2015). A majority of m6A residues are in the last exons, allowing the potential for 3' UTR regulation. Genes & Development. ISSN 0890-9369. 29(19), s. 2037–2053. doi: 10.1101/gad.269415.115.
Lauritzen, Knut Husø; Kleppa, Liv; Aronsen, Jan Magnus; Eide, Lars; Carlsen, Harald & Haugen, Øyvind Pernell [Vis alle 12 forfattere av denne artikkelen] (2015). Impaired dynamics and function of mitochondria caused by mtDNA toxicity leads to heart failure. American Journal of Physiology. Heart and Circulatory Physiology. ISSN 0363-6135. 309(3), s. H434–H449. doi: 10.1152/ajpheart.00253.2014.
Dahl, John Arne & Klungland, Arne (2014). Micro Chromatin Immunoprecipitation (μChIP) from Early Mammalian Embryos. Methods in molecular biology. ISSN 1064-3745. 1222, s. 227–245. doi: 10.1007/978-1-4939-1594-1_17.
Robertson, Adam Brian; Robertson, Julia; Fusser, Markus & Klungland, Arne (2014). Endonuclease G preferentially cleaves 5-hydroxymethylcytosine-modified DNA creating a substrate for recombination. Nucleic Acids Research (NAR). ISSN 0305-1048. 42(21), s. 13280–13293. doi: 10.1093/nar/gku1032.
Robertson, Adam Brian; Dahl, John Arne & Klungland, Arne (2014). DNA metabolism: bases of DNA repair and regulation. Nature Chemical Biology. ISSN 1552-4450. 10(7), s. 487–488. doi: 10.1038/nchembio.1553.
Nilsen, Anja; Fusser, Markus; Greggains, Gareth; Fedorcsak, Peter & Klungland, Arne (2014). ALKBH4 depletion in mice leads to spermatogenic defects. PLOS ONE. ISSN 1932-6203. 9(8). doi: 10.1371/journal.pone.0105113.
Klungland, Arne & Dahl, John Arne (2014). Dynamic RNA modifications in disease. Current Opinion in Genetics and Development. ISSN 0959-437X. 26, s. 47–52. doi: 10.1016/j.gde.2014.05.006.
Zdzalik, Daria; Vågbø, Cathrine Broberg; Kirpekar, F; Davydova, Erna; Puścian, Alicja & Maciejewska, Agnieszka M. [Vis alle 11 forfattere av denne artikkelen] (2014). Protozoan ALKBH8 Oxygenases Display both DNA Repair and tRNA Modification Activities. PLOS ONE. ISSN 1932-6203. 9(6). doi: 10.1371/journal.pone.0098729. Fulltekst i vitenarkiv
Jonson, Ida; Ougland, Rune; Klungland, Arne & Larsen, Elisabeth (2013). Oxidative stress causes DNA triplet expansion in Huntington's disease mouse embryonic stem cells. Stem Cell Research. ISSN 1873-5061. 11(3), s. 1264–1271. doi: 10.1016/j.scr.2013.08.010.
Li, Ming-Ming; Nilsen, Anja; Shi, Yue; Fusser, Markus; Ding, Yue-He & Fu, Ye [Vis alle 19 forfattere av denne artikkelen] (2013). ALKBH4-dependent demethylation of actin regulates actomyosin dynamics. Nature Communications. ISSN 2041-1723. 4. doi: 10.1038/ncomms2863.
Solberg, Anja; Robertson, Adam Brian; Aronsen, Jan Magnus; Rognmo, Øivind; Sjaastad, Ivar & Wisløff, Ulrik [Vis alle 7 forfattere av denne artikkelen] (2013). Deletion of mouse Alkbh7 leads to obesity. Journal of Molecular Cell Biology. ISSN 1674-2788. 5(3), s. 194–203. doi: 10.1093/jmcb/mjt012.
Furu, Kari & Klungland, Arne (2013). Tzfp Represses the Androgen Receptor in Mouse Testis. PLOS ONE. ISSN 1932-6203. 8(4). doi: 10.1371/journal.pone.0062314.
Zheng, Guanqun; Dahl, John Arne; Niu, Yamei; Fedorcsak, Peter; Huang, Chun-Min & Li, Charles J. [Vis alle 28 forfattere av denne artikkelen] (2013). ALKBH5 Is a Mammalian RNA Demethylase that Impacts RNA Metabolism and Mouse Fertility. Molecular Cell. ISSN 1097-2765. 49(1), s. 18–29. doi: 10.1016/j.molcel.2012.10.015.
Ougland, Rune; Lando, David; Jonson, Ida; Dahl, John Arne; Moen, Marivi & Nordstrand, Line Mari [Vis alle 11 forfattere av denne artikkelen] (2012). ALKBH1 Is a Histone H2A Dioxygenase Involved in Neural Differentiation. Stem Cells. ISSN 1066-5099. 30(12), s. 2672–2682. doi: 10.1002/stem.1228. Fulltekst i vitenarkiv
Møllersen, Linda; Rowe, Alexander D.; Illuzzi, Jennifer L.; Hildrestrand, Gunn Annette; Gerhold, Katharina J. & Tveterås, Linda Augustin [Vis alle 10 forfattere av denne artikkelen] (2012). Neil1 is a genetic modifier of somatic and germline CAG trinucleotide repeat instability in R6/1 mice. Human Molecular Genetics. ISSN 0964-6906. 21(22), s. 4939–4947. doi: 10.1093/hmg/dds337.
Kleppa, Liv; Mari, Pierre-Olivier; Larsen, Elisabeth; Lien, Guro Flor; Godon, Camille & Theil, Arjan F [Vis alle 11 forfattere av denne artikkelen] (2012). Kinetics of endogenous mouse FEN1 in base excision repair. Nucleic Acids Research (NAR). ISSN 0305-1048. 40(18), s. 9044–9059. doi: 10.1093/nar/gks673.
Meza, Trine Johansen; Moen, Marivi; Vågbø, Cathrine Broberg; Krokan, Hans Einar; Klungland, Arne & Grini, Paul Eivind [Vis alle 7 forfattere av denne artikkelen] (2012). The DNA dioxygenase ALKBH2 protects Arabidopsis thaliana against methylation damage. Nucleic Acids Research (NAR). ISSN 0305-1048. 40(14), s. 6620–6631. doi: 10.1093/nar/gks327. Fulltekst i vitenarkiv
Calvo, Jennifer A; Meira, Lisiane B.; Lee, C.Y.; Moroski-Erkul, Catherine A; Abolhassani, Nona & Taghizadeh, Koli [Vis alle 11 forfattere av denne artikkelen] (2012). DNA repair is indispensable for survival after acute inflammation. Journal of Clinical Investigation. ISSN 0021-9738. 122(7), s. 2680–2689. doi: 10.1172/JCI63338.
Robertson, Adam Brian; Dahl, John Arne; Ougland, Rune & Klungland, Arne (2012). Pull-down of 5-hydroxymethylcytosine DNA using JBP1-coated magnetic beads. Nature Protocols. ISSN 1754-2189. 7(2), s. 340–350. doi: 10.1038/nprot.2011.443.
Nordstrand, Line Mari; Furu, Kari; Paulsen, Jonas; Rognes, Torbjørn & Klungland, Arne (2012). Alkbh1 and Tzfp repress a non-repeat piRNA cluster in pachytene spermatocytes. Nucleic Acids Research (NAR). ISSN 0305-1048. 40(21), s. 10950–10963. doi: 10.1093/nar/gks839. Fulltekst i vitenarkiv
Strømme, Petter; Dobrenis, Kostantin; Sillitoe, Roy V.; Gulinello, Maria; Ali, Nafeeza F. & Davidson, Christin [Vis alle 11 forfattere av denne artikkelen] (2011). X-linked Angelman-like syndrome caused by Slc9a6 knockout in mice exhibits evidence of endosomal-lysosomal dysfunction. Brain. ISSN 0006-8950. 134, s. 3369–3383. doi: 10.1093/brain/awr250.
Lauritzen, Knut Husø; Dalhus, Bjørn; Storm, Johan Frederik; Bjørås, Magnar & Klungland, Arne (2011). Modeling the impact of mitochondrial DNA damage in forebrain neurons and beyond. Mechanisms of Ageing and Development. ISSN 0047-6374. 132(8-9), s. 424–428. doi: 10.1016/j.mad.2011.02.006.
Haj Yasein, Nadia Nabil; Vindedal, Gry Fluge; Eilert-Olsen, Martine; Gundersen, Georg Andreas; Skare, Øivind & Laake, Petter [Vis alle 11 forfattere av denne artikkelen] (2011). Glial-conditional deletion of aquaporin-4 (Aqp4) reduces blood-brain water uptake and confers barrier function on perivascular astrocyte endfeet. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 108(43), s. 17815–17820. doi: 10.1073/pnas.1110655108.
Haj Yasein, Nadia Nabil; Jensen, Vidar; Vindedal, Gry Fluge; Gundersen, Georg Andreas; Klungland, Arne & Ottersen, Ole Petter [Vis alle 8 forfattere av denne artikkelen] (2011). Evidence that Compromised K+ Spatial Buffering Contributes to the Epileptogenic Effect of Mutations in the Human Kir4.1 Gene (KCNJ10). Glia. ISSN 0894-1491. 59(11), s. 1635–1642. doi: 10.1002/glia.21205.
Lauritzen, Knut Husø; Chang, Cheng; Wiksén, Hege; Bergersen, Linda Hildegard & Klungland, Arne (2011). Mitochondrial DNA toxicity compromises mitochondrial dynamics and induces hippocampal antioxidant defenses. DNA Repair. ISSN 1568-7864. 10(6), s. 639–653. doi: 10.1016/j.dnarep.2011.04.011.
Robertson, Julia; Robertson, Adam Brian & Klungland, Arne (2011). The presence of 5-hydroxymethylcytosine at the gene promoter and not in the gene body negatively regulates gene expression. Biochemical and Biophysical Research Communications - BBRC. ISSN 0006-291X. 411(1), s. 40–43. doi: 10.1016/j.bbrc.2011.06.077.
Moen, Marivi; Knævelsrud, Ingeborg; Haugland, Gyri Teien; Grøsvik, Kristin; Birkeland, Nils-Kåre & Klungland, Arne [Vis alle 7 forfattere av denne artikkelen] (2011). Uracil-DNA Glycosylase of Thermoplasma acidophilum Directs Long-Patch Base Excision Repair, Which Is Promoted by Deoxynucleoside Triphosphates and ATP/ADP, into Short-Patch Repair. Journal of Bacteriology. ISSN 0021-9193. 193(17), s. 4495–4508. doi: 10.1128/JB.00233-11.
Van den Born, Erwin; Vågbø, Cathrine Broberg; Songe-Møller, Lene; Leihne, VIbeke; Lien, Guro Flor & Leszczynska, G [Vis alle 11 forfattere av denne artikkelen] (2011). ALKBH8-mediated formation of a novel diastereomeric pair of wobble nucleosides in mammalian tRNA. Nature Communications. ISSN 2041-1723. 2. doi: 10.1038/ncomms1173. Fulltekst i vitenarkiv
Robertson, Adam Brian; Dahl, John Arne; Vågbø, Cathrine Broberg; Tripathi, P; Krokan, Hans Einar & Klungland, Arne (2011). A novel method for the efficient and selective identification of 5-hydroxymethylcytosine in genomic DNA. Nucleic Acids Research (NAR). ISSN 0305-1048. 39(8). doi: 10.1093/nar/gkr051. Fulltekst i vitenarkiv
Fusser, Markus; Nesse, Gaute; Khobta, Andriy; Xia, Ning; Li, Huige & Klungland, Arne [Vis alle 7 forfattere av denne artikkelen] (2011). Spontaneous mutagenesis in Csb(m/m) Ogg1(−/−) mice is attenuated by dietary resveratrol. Carcinogenesis. ISSN 0143-3334. 32(1), s. 80–85. doi: 10.1093/carcin/bgq196.
Thrane, Alexander Stanley; Rappold, Philip M; Fujita, Takumi; Torres, Arnulfo; Bekar, Lane K & Takano, Takahiro [Vis alle 17 forfattere av denne artikkelen] (2011). Critical role of aquaporin-4 (AQP4) in astrocytic Ca2+ signaling events elicited by cerebral edema. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 108(2), s. 846–851. doi: 10.1073/pnas.1015217108.
Nordstrand, Line Mari; Svärd, Jessica; Larsen, Elisabeth; Nilsen, Anja; Ougland, Rune & Furu, Kari [Vis alle 11 forfattere av denne artikkelen] (2010). Mice Lacking Alkbh1 Display Sex-Ratio Distortion and Unilateral Eye Defects. PLOS ONE. ISSN 1932-6203. 5(11). doi: 10.1371/journal.pone.0013827. Fulltekst i vitenarkiv
Møllersen, Linda; Rowe, Alexander D.; Larsen, Elisabeth; Rognes, Torbjørn & Klungland, Arne (2010). Continuous and Periodic Expansion of CAG Repeats in Huntington's Disease R6/1 Mice. PLoS Genetics. ISSN 1553-7390. 6(12). doi: 10.1371/journal.pgen.1001242. Fulltekst i vitenarkiv
Knævelsrud, Ingeborg; Moen, Marivi; Grøsvik, Kristin; Haugland, Gyri Teien; Birkeland, Nils-Kåre & Klungland, Arne [Vis alle 8 forfattere av denne artikkelen] (2010). The Hyperthermophilic Euryarchaeon Archaeoglobus fulgidus Repairs Uracil by Single-Nucleotide Replacement. Journal of Bacteriology. ISSN 0021-9193. 192(21), s. 5755–5766. doi: 10.1128/JB.00135-10. Vis sammendrag
Hydrolytic deamination of cytosine to uracil in cellular DNA is a major source of C-to-T transition mutations if uracil is not repaired by the DNA base excision repair (BER) pathway. Since deamination increases rapidly with temperature, hyperthermophiles, in particular, are expected to succumb to such damage. There has been only one report of crenarchaeotic BER showing strong similarities to that in most eukaryotes and bacteria for hyperthermophilic Archaea. Here we report a different type of BER performed by extract prepared from cells of the euryarchaeon Archaeoglobus fulgidus. Although immunodepletion showed that the monofunctional family 4 type of uracil-DNA glycosylase (UDG) is the principal and probably only UDG in this organism, a beta-elimination mechanism rather than a hydrolytic mechanism is employed for incision of the abasic site following uracil removal. The resulting 3' remnant is removed by efficient 3'-phosphodiesterase activity followed by single-nucleotide insertion and ligation. The finding that repair product formation is stimulated similarly by ATP and ADP in vitro raises the question of whether ADP is more important in vivo because of its higher heat stability.
Dahl, John Arne; Reiner, Andrew Henry; Klungland, Arne; Wakayama, Teruhiko & Collas, Philippe (2010). Histone H3 Lysine 27 Methylation Asymmetry on Developmentally-Regulated Promoters Distinguish the First Two Lineages in Mouse Preimplantation Embryos. PLOS ONE. ISSN 1932-6203. 5(2). doi: 10.1371/journal.pone.0009150.
Songe-Møller, Lene; Van den Born, Erwin; Leihne, V; Vågbø, Cathrine Broberg; Kristoffersen, T & Krokan, Hans Einar [Vis alle 9 forfattere av denne artikkelen] (2010). Mammalian ALKBH8 Possesses tRNA Methyltransferase Activity Required for the Biogenesis of Multiple Wobble Uridine Modifications Implicated in Translational Decoding. Molecular and Cellular Biology. ISSN 0270-7306. 30(7), s. 1814–1827. doi: 10.1128/MCB.01602-09.
Lauritzen, Knut Husø; Moldestad, Olve; Eide, Lars; Carlsen, Harald; Nesse, Gaute & Storm, Johan Frederik [Vis alle 9 forfattere av denne artikkelen] (2010). Mitochondrial DNA Toxicity in Forebrain Neurons Causes Apoptosis, Neurodegeneration, and Impaired Behavior. Molecular and Cellular Biology. ISSN 0270-7306. 30(6), s. 1357–1367. doi: 10.1128/MCB.01149-09. Vis sammendrag
Mitochondrial dysfunction underlying changes in neurodegenerative diseases is often associated with apoptosis and a progressive loss of neurons, and damage to the mitochondrial genome is proposed to be involved in such pathologies. In the present study we designed a mouse model that allows us to specifically induce mitochondrial DNA toxicity in the forebrain neurons of adult mice. This is achieved by CaMKII-regulated inducible expression of a mutated version of the mitochondrial UNG DNA repair enzyme (mutUNG1). This enzyme is capable of removing thymine from the mitochondrial genome. We demonstrate that a continual generation of apyrimidinic sites causes apoptosis and neuronal death. These defects are associated with behavioral alterations characterized by increased locomotor activity, impaired cognitive abilities, and lack of anxietylike responses. In summary, whereas mitochondrial base substitution and deletions previously have been shown to correlate with premature and natural aging, respectively, we show that a high level of apyrimidinic sites lead to mitochondrial DNA cytotoxicity, which causes apoptosis, followed by neurodegeneration.
Robertson, AB; Klungland, A; Rognes, T & Leiros, Ingar (2009). DNA Repair in Mammalian Cells. Cellular and Molecular Life Sciences (CMLS). ISSN 1420-682X. 66(6), s. 981–993. doi: 10.1007/s00018-009-8736-z.
Van den Born, Erwin; Bekkelund, Anders; Moen, Marivi; Omelchenko, M.V.; Klungland, Arne & Falnes, Pål (2009). Bioinformatics and functional analysis define four distinct groups of AlkB DNA-dioxygenases in bacteria. Nucleic Acids Research (NAR). ISSN 0305-1048. 37(21). doi: 10.1093/nar/gkp774.
Ringvoll, Jeanette; Moen, Marivi N.; Nordstrand, Line M.; Meira, Lisiane B.; Pang, Bo & Bekkelund, Anders [Vis alle 11 forfattere av denne artikkelen] (2008). AlkB homologue 2-mediated repair of ethenoadenine lesions in mammalian DNA. Cancer Research. ISSN 0008-5472. 68(11), s. 4142–4149. Vis sammendrag
Endogenous formation of the mutagenic DNA adduct 1,N-6-ethenoadenine (epsilon A) originates from lipid peroxidation. Elevated levels of epsilon A in cancer-prone tissues suggest a role for this adduct in the development of some cancers. The base excision repair pathway has been considered the principal repair system for epsilon A lesions until recently, when it was shown that the Escherichia coli AlkB dioxygenase could directly reverse the damage. We report here kinetic analysis of the recombinant human AlkB homologue 2 (hABH2), which is able to repair epsilon A lesions in DNA. Furthermore, cation exchange chromatography of nuclear extracts from wild-type and mABH2(-/-) mice indicates that mABH2 is the principal dioxygenase for EA repair in vivo. This is further substantiated by experiments showing that hABH2, but not hABH3, is able to complement the E. coli alkB mutant with respect to its defective repair of etheno adducts. We conclude that ABH2 is active in the direct reversal of epsilon A lesions, and that ABH2, together with the alkyl-N-adenine-DNA glycosylase, which is the most effective enzyme for the repair of epsilon A, comprise the cellular defense against epsilon A lesions.
Larsen, Elisabeth; Kleppa, Liv; Meza, Trine Johansen; Meza, Zepeda Leonardo; Rada, C & Castellanos, Cesilie Granum [Vis alle 12 forfattere av denne artikkelen] (2008). Early-onset lymphoma and extensive embryonic apoptosis in two domain-specific Fen1 mice mutants. Cancer Research. ISSN 0008-5472. 68, s. 4571–4579. doi: 10.1158/0008-5472.CAN-08-0168.
Saxowsky, TT; Meadows, KL; Klungland, Arne & Doetsch, PW (2008). 8-Oxoguanine-mediated transcriptional mutagenesis causes Ras activation in mammalian cells. Proceedings of the National Academy of Sciences of the United States of America. ISSN 0027-8424. 105(48), s. 18877–18882. doi: 10.1073/pnas.0806464105.
Wærsted, Jeanette Ringvoll; Nabong, Marivi; Nordstrand, Line Mari; Meira, LB; Pang, B & Bekkelund, Anders [Vis alle 11 forfattere av denne artikkelen] (2008). AlkB homologue 2-mediated repair of ethenoadenine lesions in mammalian DNA. Cancer Research. ISSN 0008-5472. 68(11), s. 4142–4149. doi: 10.1158/0008-5472.CAN-08-0796. Vis sammendrag
Endogenous formation of the mutagenic DNA adduct 1,N6-ethenoadenine ({varepsilon}A) originates from lipid peroxidation. Elevated levels of {varepsilon}A in cancer-prone tissues suggest a role for this adduct in the development of some cancers. The base excision repair pathway has been considered the principal repair system for {varepsilon}A lesions until recently, when it was shown that the Escherichia coli AlkB dioxygenase could directly reverse the damage. We report here kinetic analysis of the recombinant human AlkB homologue 2 (hABH2), which is able to repair {varepsilon}A lesions in DNA. Furthermore, cation exchange chromatography of nuclear extracts from wild-type and mABH2–/– mice indicates that mABH2 is the principal dioxygenase for {varepsilon}A repair in vivo. This is further substantiated by experiments showing that hABH2, but not hABH3, is able to complement the E. coli alkB mutant with respect to its defective repair of etheno adducts. We conclude that ABH2 is active in the direct reversal of {varepsilon}A lesions, and that ABH2, together with the alkyl-N-adenine-DNA glycosylase, which is the most effective enzyme for the repair of {varepsilon}A, comprise the cellular defense against {varepsilon}A lesions. [Cancer Res 2008;68(11):4142–9]
Leiros, Ingar; Nabong, Marivi P.; Grøsvik, Kristin; Ringvoll, Jeanette; Haugland, GT & Uldal, Lene [Vis alle 15 forfattere av denne artikkelen] (2007). Structural basis for enzymatic excision of N-1-methyladenine and N-3-methylcytosine from DNA. EMBO Journal. ISSN 0261-4189. 26. Vis sammendrag
N-1-methyladenine (m(1)A) and N-3-methylcytosine (m(3)C) are major toxic and mutagenic lesions induced by alkylation in single-stranded DNA. In bacteria and mammals, m 1 A and m 3 C were recently shown to be repaired by AlkB-mediated oxidative demethylation, a direct DNA damage reversal mechanism. No AlkB gene homologues have been identified in Archaea. We report that m(1)A and m(3)C are repaired by the AfAlkA base excision repair glycosylase of Archaeoglobus fulgidus, suggesting a different repair mechanism for these lesions in the third domain of life. In addition, AfAlkA was found to effect a robust excision of 1, N-6-ethenoadenine. We present a high-resolution crystal structure of AfAlkA, which, together with the characterization of several site-directed mutants, forms a molecular rationalization for the newly discovered base excision activity.
Klungland, Arne & Bjelland, Svein (2007). Oxidative damage to purines in DNA: Role of mammalian Ogg1. DNA Repair. ISSN 1568-7864. 6. Vis sammendrag
DNA damage caused by reactive oxygen species is ubiquitous to all living organisms. More than 60 different base lesions have been identified, and the majority of these are removed via the base excision repair pathway. This pathway appears to represent a highly conserved and ancient mechanism of defence counteracting spontaneous DNA decay. In this review, we describe in more detail the Ogg1 enzyme and its conserved action of removing the oxidised base, 7,8-dihydro-8-oxoguanine (oxo(8)G). Recent updates include the cancer-prone ogg1/myh double knockout mouse and an elegant study which looks at the ability of hOgg1 to distinguish between the mutagenic lesion, oxo(8)G, and the vast majority of normal bases. (c) 2006 Elsevier B.V. All rights reserved.
Klungland, Arne; Lærdahl, Jon Kristen & Rognes, Torbjørn (2007). OGG1: From Structural Analysis to the Knockout Mouse. I Evans, Mark D. & Cooke, Marcus S. (Red.), Oxidative Damage to Nucleic Acids. Springer Science+Business Media B.V.. ISSN 9780387729732. s. 67–80.
Larsen, E; Meza, TJ; Kleppa, L & Klungland, Arne (2007). Organ and cell specificity of base excision repair mutants in mice. Mutation research. ISSN 0027-5107. 614, s. 56–68. doi: 10.1016/j.mrfmmm.2006.01.023.
Nordstrand, Line Mari; Ringvoll, Jeanette; Larsen, Elisabeth & Klungland, Arne (2007). Genome instability and DNA damage accumulation in gene-targeted mice. Neuroscience. ISSN 0306-4522. 145. doi: 10.1016/j.neuroscience.2006.10.059.
Falnes, Pål; Klungland, Arne & Alseth, Ingrun (2007). Repair of methyl lesions in DNA and RNA by oxidative demethylation. Neuroscience. ISSN 0306-4522. 145. doi: 10.1016/j.neuroscience.2006.11.018.
Ringvoll, Jeanette; Uldal, Lene; Røed, Marie Aslaksen; Stenersen, Karen Oppland; Baynton, K & Klungland, Arne [Vis alle 7 forfattere av denne artikkelen] (2007). Mutations in the RAD27 and SGS1 genes differentially affect the chronological and replicative lifespan of yeast cells growing on glucose and glycerol. FEMS Yeast Research. ISSN 1567-1356. 7, s. 848–859. doi: 10.1111/j.1567-1364.2007.00248.x.
Kovtun, IV; Liu, Y; Bjørås, Magnar; Klungland, Arne; Wilson, SH & McMurray, CT (2007). OGG1 initiates age-dependent CAG trinucleotide expansion in somatic cells. Nature. ISSN 0028-0836. 447. doi: 10.1038/nature05778.
Leiros, Ingar; Nabong, MP; Nabong, Marivi; Grosvik, K; Grøsvik, Kristin & Ringvoll, J [Vis alle 20 forfattere av denne artikkelen] (2007). Structural basis for enzymatic excision of N-1-methyladenine and N-3-methylcytosine from DNA. EMBO Journal. ISSN 0261-4189. 26. doi: 10.1038/sj.emboj.7601662.
Kleppa, Liv; Kanavin, Øivind Juris; Klungland, Arne & Strømme, Petter (2007). A novel splice site mutation in the Cockayne syndrome group A gene in two siblings with Cockayne syndrome. Neuroscience. ISSN 0306-4522. 4, s. 1397–1406. doi: 10.1016/j.neuroscience.2006.09.025.
Larsen, Elisabeth; Stenersen, Karen Oppland; Nesse, Gaute; Neurauter, Christine Gran; Seeberg, Erling Christen & Klungland, Arne (2006). Repair and mutagenesis at oxidized DNA lesions in the developing brain of wild-type and Ogg1(-/-) mice. Oncogene. ISSN 0950-9232. 25, s. 2425–2432.
Ringvoll, Jeanette; Ringvoll, Jeanette; Nordstrand, Line M.; Vågbø, Cathrine Broberg; Vagbo, CB & Talstad, Vivi [Vis alle 15 forfattere av denne artikkelen] (2006). Repair deficient mice reveal mABH2 as the primary oxidative demethylase for repairing 1meA and 3meC lesions in DNA. EMBO Journal. ISSN 0261-4189. 25, s. 2189–2198.
Touati, E; Michel, V; Thiberge, JM; Ave, P; Huerre, M & Bourgade, F [Vis alle 8 forfattere av denne artikkelen] (2006). Deficiency in OGG1 protects against inflammation and mutagenic effects associated with H-Pylori infection in mouse. Helicobacter. ISSN 1083-4389. 11, s. 494–505.
Kleppa, Liv; Kanavin, Ø.J; Klungland, Arne & Strømme, Petter (2006). A novel splice site mutation in the Cockayne syndrome group A gene in two siblings with Cockayne syndrome. Neuroscience. ISSN 0306-4522. doi: 10.1016/j.neuroscience.
Holen, Torgeir; Moe, Svein Erik E; Sørbø, Jan Gunnar; Meza, Trine Johansen; Ottersen, Ole Petter & Klungland, Arne (2005). Tolerated wobble mutations in siRNAs decrease specificity, but can enhance activity in vivo. Nucleic Acids Research (NAR). ISSN 0305-1048. 33(15), s. 4704–4710. doi: 10.1093/nar/gki785.
Rozalski, R; Siomek, A; Gackowski, D; Foksinski, M; Gran, C & Klungland, Arne [Vis alle 7 forfattere av denne artikkelen] (2005). Substantial decrease of urinary 8-oxo-7,8-dihydroguanine, a product of the base excision repair pathway, in DNA glycosylase defective mice. International Journal of Biochemistry and Cell Biology. ISSN 1357-2725. 37.
Rozalski, R; Siomek, A; Gackowski, D; Gran, Christine; Foksinski, M & Gran, C [Vis alle 8 forfattere av denne artikkelen] (2004). Diet is not responsible for the presence of several oxidatively damaged DNA lesions in mouse urine. Free radical research. ISSN 1071-5762. 38(11), s. 1201–1205.
Foksinski, M; Rozalski, R; Guz, J; Ruszkowska, B; Sztukowska, P & Piwowarski, M [Vis alle 8 forfattere av denne artikkelen] (2004). Urinary excretion of DNA repair products correlates with metabolic rates as well as with maximum life spans of different mammalian species. Free Radical Biology & Medicine. ISSN 0891-5849. 37(9), s. 1449–1454.
Larsen, E; Kwon, K; Coin, F; Egly, JM & Klungland, Arne (2004). Transcription activities at 8-oxoG lesions in DNA. DNA Repair. ISSN 1568-7864. 3(11), s. 1457–1468.
Birkeland, Nils-Kåre; Ånensen, Hilde; Knævelsrud, Ingeborg; Kristoffersen, Wenche; Bjørås, M. & Robb, F.T. [Vis alle 8 forfattere av denne artikkelen] (2002). Methylpurine DNA glycosylase of the hyperthermophilic archaeon Archaeoglobus fulgidus. Biochemistry. ISSN 0006-2960. 41, s. 12697–12705.
Birkeland, Nils-Kåre; Ånensen, Hilde; Knævelsrud, Ingeborg; Kristoffersen, Wenche; Bjørås, M. & Robb, F.T. [Vis alle 8 forfattere av denne artikkelen] (2002). Methylpurine DNA glycosylase of the hyperthermophilic archaeon Archaeoglobus fulgidus. Biochemistry. ISSN 0006-2960. 41, s. 12697–12705.
Bjelland, Svein; Klungland, Arne; Paulsen, R.; Rolseth, V.; Yamada, Y. & Ueno, Y. [Vis alle 9 forfattere av denne artikkelen] (2001). 5-Formyluracil and its nucleoside derivatives confer toxicity and mutagenicity to mammalian cells by interfering with normal RNA and DNA metabolism. Toxicology Letters. ISSN 0378-4274. 119, s. 71–78.
Klungland, Helge & Klungland, Arne (2000). År 2000-2100: Genetikkens århundre? Kirke og kultur. ISSN 0023-186X. 105(05.jun), s. 461–476.

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Li, Miaomiao; Klungland, Arne & Dalhus, Bjørn (2022). Studies on Protein-RNA:DNA Hybrid Interactions by Microscale Thermophoresis (MST). Methods in molecular biology. ISSN 1064-3745. 2528, s. 239–251. doi: 10.1007/978-1-0716-2477-7_15.
Tesfahun, Almaz Nigatu; Alexeeva, Marina; Tomkuviene, Migle; Laerdahl, Jon Kristen; Klungland, Arne & Klimašauskas, Saulius [Vis alle 7 forfattere av denne artikkelen] (2018). Formamidopyrimidine-DNA glycosylase is involved in C:C mismatch repair.
Tesfahun, Almaz Nigatu; Guragain, Prashanna; Alexeeva, Marina; Arshad, Aysha; Tomkuviene, Migle & Laerdahl, Jon K. [Vis alle 9 forfattere av denne artikkelen] (2017). Repair of dimethylated bases in DNA.
Tesfahun, Almaz Nigatu; Alexeeva, Marina; Guragain, Prashanna; Tomkuviene, Migle; Arshad, Aysha & Klungland, Arne [Vis alle 8 forfattere av denne artikkelen] (2017). Repair of double methylated bases in DNA.
Alexeeva, Marina; Moen, Marivi Nabong; Xu, Xiang Ming; Rasmussen, Anette; Kirpekar, Finn & Klungland, Arne [Vis alle 7 forfattere av denne artikkelen] (2017). hSMUG1 is a bi-functional DNA glycosylase.
Tesfahun, Almaz Nigatu; Klungland, Arne; Klimasauskas, Saulius & Bjelland, Svein (2017). Repair of double methylated bases in DNA.
Tesfahun, Almaz Nigatu; Klungland, Arne; Klimašauskas, Saulius & Bjelland, Svein (2016). Repair of double methylated bases in DNA.
Alexeeva, Marina; Moen, Marivi Nabong; Xu, Xiang Ming; Rasmussen, Anette; Kirpekar, Finn & Klungland, Arne [Vis alle 7 forfattere av denne artikkelen] (2016). hSMUG1-catalysed uracil-DNA strand incision.
Alexeeva, Marina; Moen, Marivi Nabong; Xu, Xiang Ming; Rasmussen, Anette; Kirpekar, Finn & Klungland, Arne [Vis alle 7 forfattere av denne artikkelen] (2016). hSMUG1-catalysed uracil-DNA strand incision.
Tesfahun, Almaz Nigatu; Klungland, Arne; Klimasauskas, Saulius & Bjelland, Svein (2016). Repair of double methylated bases in DNA.
Catterall, Tara Cherie; Johnson, Inna; Khan, Shaista; Jermstad, Ingunn; Nesse, Gaute & Klungland, Arne [Vis alle 7 forfattere av denne artikkelen] (2015). The Norwegian Transgenic Centre.
Khan, Shaista; Jermstad, Ingunn; Catterall, Tara Cherie; Nesse, Gaute; Klungland, Arne & Dalen, Knut Tomas (2014). The Norwegian Transgenic Centre.
Rasmussen, Erik; Vågbø, Cathrine Broberg; Dahl, John Arne; Münch, Daniel; Krokan, Hans Einar & Klungland, Arne [Vis alle 7 forfattere av denne artikkelen] (2014). Epigenetically modified cytosines in the honey bee genome.
Zheng, Guanqun; Dahl, John Arne; Niu, Yamei; Fu, Ye & Klungland, Arne (2013). Sprouts of RNA epigenetics The discovery of mammalian RNA demethylases. RNA Biology. ISSN 1547-6286. 10(6), s. 915–918. doi: 10.4161/rna.24711.
Rasmussen, Erik; Vågbø, Cathrine Broberg; Dahl, John Arne; Münch, Daniel; Krokan, Hans Einar & Klungland, Arne [Vis alle 7 forfattere av denne artikkelen] (2013). Presence of modified cytosines in the honey bee genome.
Warken, D.; Khobta, Andriy; Klungland, Arne & Epe, B. (2010). Flap endonuclease 1 (FEN-1) takes part in repair of DNA single-strand breaks. Naunyn-Schmiedeberg's Archives of Pharmacology. ISSN 0028-1298. 381, s. 73–73.
Falnes, Pål; Van den Born, Erwin; Leihne, VIbeke; Meza, Trine Johansen; Songe-Møller, Lene & Klungland, Arne (2009). AlkB-like dioxygenases-roles in nucleic acid repair and modification.
Klungland, Arne & Andersson, Kristin Brevik (2007). Nobelpris for genmodifisering. Tidsskrift for Den norske legeforening. ISSN 0029-2001. 23, s. 127–127.
Leiros, Ingar; Nabong, Marivi P.; Haugland, GT; Ringvoll, Jeanette; Grøsvik, K & Grøsvik, K [Vis alle 15 forfattere av denne artikkelen] (2006). Excision of 1-methyladenine and 3-methylcytosine from DNA by AfAlkA of Archaeoglobus fulgidus.
Trapp, C; Klungland, Arne & Epe, B (2006). The peroxisome proliferator WY-14,643 does not induce oxidative DNA damage in repair-deficient Csb(-/-)Ogg1(-/-) knockout mice. Naunyn-Schmiedeberg's Archives of Pharmacology. ISSN 0028-1298. 372, s. 112–112.
Leiros, Ingar; Nabong, Marivi P.; Haugland, Gyri Teien; Ringvoll, Jeanette; Grøsvik, Kristin & Moe, Elin [Vis alle 12 forfattere av denne artikkelen] (2006). Excision of 1-methyladenine and 3-methylcytosine from DNA by AfAlkA of Archaeoglobus fulgidus.
Meza, Trine Johansen; Elisabeth, Larsen; Nesse, Gaute & Klungland, Arne (2005). Knock-down studies of FEN1 by gene targeting and RNAi.
Bjelland, Svein; Knævelsrud, Ingeborg; Haugland, Gyri Teien; Klungland, Arne & Birkeland, Nils-Kåre (2005). Excision of dUMP from DNA by the hyperthermophilic archaeon Archaeoglobus fulgidus.
Meza, Trine Johansen; Elisabeth, Larsen; Halsne, Ruth & Klungland, Arne (2004). Knock-down studies of FEN1 by gene targeting and RNAi.
Birkeland, Nils-Kåre; Ånensen, Hilde; Kristoffersen, Wenche; Knævelsrud, Ingeborg; Bjørås, M. & Klungland, Arne [Vis alle 8 forfattere av denne artikkelen] (2002). Cold-active methylpurine DNA glycosylase of the hyperthermophilic archaeon Archaeoglobus fulgidus.
Birkeland, Nils-Kåre; Ånensen, Hilde; Kristoffersen, Wenche; Knævelsrud, Ingeborg; Bjørås, M. & Klungland, Arne [Vis alle 8 forfattere av denne artikkelen] (2002). Cold-active methylpurine DNA glycosylase of the hyperthermophilic archaeon Archaeoglobus fulgidus.
Klungland, Helge; Klungland, Arne & Kjær, Jan Erik (1998). Gener i avlsarbeidet. Buskap. ISSN 0007-7194. 50(5), s. 20–22.
Klungland, Arne; Klungland, Helge & Kjær, Jan Erik (1998). Derfor er dyrene forskjellige. Buskap. ISSN 0007-7194. 50(4), s. 10–11.
Klungland, Helge; Klungland, Arne & Kjær, Jan Erik (1998). Gener og kromosomer. Buskap. ISSN 0007-7194. 50(3), s. 36–37.

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Publisert 13. apr. 2011 10:38 - Sist endret 24. jan. 2024 09:31

Forskergrupper

Dynamisk genregulering