Forskning
Vår forskningsgruppe studerer NK-celler og "innate lymphoid cells" med et fokus på hvordan NK-celler er regulert av kreft, og hvordan de kan benyttes terapeutisk i forbindelse med beinmargstransplantasjon og akutte leukemier.
Prosjekter
- Prospektive studer av NK-celler i barn med ALL eller AML hvor vi korrelerer NK-celleresponser/fenotyper med risiko for tilbakefall, og også interaksjoner mellom leukemien og NK-cellene.
- Mekanistiske studier av hvordan leukemi kan hemme immunsystemet ved bruk av dyremodeller for ALL og AML, inkludert ex vivo aktivering av NK-celler for immunterapi, og studier av exosomer frigjort fra leukemiceller.
- Karakterisering av NK-celler og ILC i rotte, og hvordan de reguleres i forbindelse med akutt GvHD etter beinmargstransplantasjon.
- Karakterisering av intreacellulære signalkaskader i humane NK-celler
Emneord:
NK-celler,
Immunologi,
Exosomer,
Transplantasjonsimmunologi
Publikasjoner
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Bräunlich, Paula Marie; Inngjerdingen, Kari Tvete; Inngjerdingen, Marit; Johnson, Quinton; Paulsen, Berit Smestad & Mabusela, Wilfred (2018). Polysaccharides from the South African medicinal plant Artemisia afra: Structure and activity studies. Fitoterapia.
ISSN 0367-326X.
124, s 182- 187 . doi:
10.1016/j.fitote.2017.11.016
Fulltekst i vitenarkiv.
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Sikorski, Krzysztof; Mehta, Adi; Inngjerdingen, Marit; Thakor, Flourina Kumar; Kling, Simon; Kalina, Tomas; Nyman, Tuula Anneli; Stensland, Maria; Zhou, Wei; De Souza, Gustavo Antonio; Holden, Lars; Stuchly, Jan; Templin, Markus & Lund-Johansen, Fridtjof (2018). A high-throughput pipeline for validation of antibodies. Nature Methods.
ISSN 1548-7091.
15(11), s 909- 912 . doi:
10.1038/s41592-018-0179-8
Fulltekst i vitenarkiv.
Vis sammendrag
Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we used preparative gel electrophoresis to develop a capture format for WB. Fractions with soluble, size-separated proteins facilitated parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). This pipeline provided the means for large-scale implementation of antibody validation concepts proposed by an international working group on antibody validation (IWGAV).Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we used preparative gel electrophoresis to develop a capture format for WB. Fractions with soluble, size-separated proteins facilitated parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). This pipeline provided the means for large-scale implementation of antibody validation concepts proposed by an international working group on antibody validation (IWGAV).
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Boieri, Margherita; Shah, Pranali; Jalapothu, Dasaradha; Zaitseva, Olena; Walter, Lutz; Rolstad, Bent; Naper, Christian; Dressel, Ralf & Inngjerdingen, Marit (2017). Rat acute GvHD is Th1 driven and characterized by predominant donor CD4+ T-cell infiltration of skin and gut. Experimental Hematology.
ISSN 0301-472X.
50, s 33- 45.e3 . doi:
10.1016/j.exphem.2017.02.002
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Boieri, Margherita; ULVMOEN, AINA; Sudworth, Amanda; Lendrem, Clare; Collin, Matthew; Dickinson, Anne M.; Kveberg, Lise & Inngjerdingen, Marit (2017). IL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia development in vivo. Oncoimmunology.
ISSN 2162-4011.
6(3:e1274478), s 1- 13 . doi:
10.1080/2162402X.2016.1274478
Fulltekst i vitenarkiv.
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Sudworth, Amanda; Vaage, John T.; Inngjerdingen, Marit & Kveberg, Lise (2017). Frontline Science: A hyporesponsive subset of rat NK cells negative for Ly49s3 and NKR-P1B are precursors to the functionally mature NKR-P1B+ subset. Journal of Leukocyte Biology.
ISSN 0741-5400.
102(6), s 1289- 1298 . doi:
10.1189/jlb.1HI0517-177RR
Fulltekst i vitenarkiv.
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Boieri, Margherita; Shah, Pranali; Dressel, Ralf & Inngjerdingen, Marit (2016). The role of animal models in the study of hematopoietic stem cell transplantation and GvHD: A historical overview. Frontiers in Immunology.
ISSN 1664-3224.
7:333 . doi:
10.3389/fimmu.2016.00333
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Jalapothu, Dasaradha; Boieri, Margherita; Crossland, Rachel E.; Shah, Pranali; Butt, Isha Ammar; Norden, Jean; Dressel, Ralf; Dickinson, Anne M. & Inngjerdingen, Marit (2016). Tissue-specific expression patterns of MicroRNA during acute graft-versus-host disease in the rat. Frontiers in Immunology.
ISSN 1664-3224.
7:361 . doi:
10.3389/fimmu.2016.00361
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Lund-Johansen, Fridtjof; de la Rosa-Carrillo, Daniel; Mehta, Adi; Sikorski, Krzysztof; Inngjerdingen, Marit; Kalina, Tomáš; Røysland, Kjetil; De Souza, Gustavo Antonio; Bradbury, Andrew R.M.; Lecrevisse, Quentin & Stuchly, Jan (2016). MetaMass, a tool for meta-analysis of subcellular proteomics data. Nature Methods.
ISSN 1548-7091.
13(10), s 837- 840 . doi:
10.1038/nmeth.3967
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Kveberg, Lise; Sudworth, Amanda; Todros-Dawda, Izabela Malgorzata; Inngjerdingen, Marit & Vaage, John Torgils (2015). Functional characterization of a conserved pair of NKR-P1 receptors expressed by NK cells and T lymphocytes in liver and gut. European Journal of Immunology.
ISSN 0014-2980.
45(2), s 501- 512 . doi:
10.1002/eji.201444710
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Le Normand, Myriam; Melida, Hugo; Holmbom, Bjarne; Michaelsen, Terje Einar; Inngjerdingen, Marit; Bulone, Vincent; Paulsen, Berit Smestad & Ek, Monica (2014). Hot-water extracts from the inner bark of Norway spruce with immunomodulating activities. Carbohydrate Polymers.
ISSN 0144-8617.
101, s 699- 704 . doi:
10.1016/j.carbpol.2013.09.067
Se alle arbeider i Cristin
Publisert 28. sep. 2018 11:41
- Sist endret 19. des. 2018 08:34
