Shuo-Wang Qiao

Bilde av Shuo-Wang Qiao
English version of this page
Telefon +47-22850533
Mobiltelefon +47-97725876
Rom A2.2053
Brukernavn
Besøksadresse Sognsvannsveien 20 Rikshospitalet 0372 Oslo
Postadresse Postboks 4950 Nydalen 0424 OSLO

Jeg er utdannet cand. med. og dr. med. fra Universitetet i Oslo. I tillegg har jeg en cand. mag. grad i matematikk. Jeg har jobbet ved Immunologisk institutt, Rikshospitalet siden 2001 foruten et to-årlig post doc i USA.

Mine faglige interesser 

Under doktorgraden jobbet jeg med identifisering og karakterisering av T-celleepitoper i gluten som har betydning for cøliakiutvikling. Under post doc oppholdet undersøkte jeg betydning av neonatal Fc reseptor (FcRn) i antigenpresentasjon, spesielt antigen som er bundet til antistoffer i store komplekser. I de siste ti årene har jeg jobbet molekylært, med analyse av T-cellereseptorer og hel transkriptom på enkeltcelle-nivå. Disse analysene utføres på både gluten-spesifikke T-celler som driver cøliakiutvikling og andre immunceller som driver kroniske inflammatoriske sykdommer.  Jeg har vært gruppeleder i Centre for Immune Regulation (CIR) som var et SFF i perioden 2007-2017. Siden 2016 har jeg vært gruppeleder i K. G. Jebsen senter for cøliakiforskning. Ved siden av forskning av human immunologi, jobber jeg i et samarbeidsprosjekt finansiert av konvergensmidler fra UiO: Livsvitenskap med å kartlegge det særegne immunsystemet hos torsk.

Bakgrunn

  • Gruppeleder, K. G. Jebsen senter for cøliakiforskning fra 2016
  • Gruppeleder, Centre for Immune Regulation 2015-2017
  • Førsteamanuensis, avdeling for immunologi og transfusjonsmedisin fra 2015
  • Undervisning av adaptiv immunologi for medisinerstudenter, fra 2015
  • Forsker, Immunologisk institutt, Rikshospitalet, 2010-2015
  • Post doc med NFR stipend, Immunologisk institutt, Rikshospitalet, 2007-2009
  • Post doc, Brigham and Women’s hospital og Harvard Medical School, 2005-2007
  • Dr. med., Immunologisk institutt, UiO, 2005
  • Styremedlem i EuroDoc, 2004
  • Styremedlem i UiODoc og Stipendiatorganisasjonene i Norge, 2003-2004
  • Cand. mag. (i matematikk), UiO, 2001
  • Cand. med., UiO, 1999
Emneord: Immunologi, T-celler, Autoimmune sykdommer, Molekylærbiologi, scRNA-seq

Publikasjoner

  • Bartolomé Casado, Raquel; Landsverk, Ole J. B.; Chauhan, Sudhir Kumar; Richter, Lisa Maria; Phung, Danh; Greiff, Victor; Risnes, Louise Fremgaard; Yao, Ying; Neumann, Ralf Stefan; Yaqub, Sheraz; Øyen, Ole; Horneland, Rune; Aandahl, Einar Martin; Paulsen, Vemund; Sollid, Ludvig Magne; Qiao, Shuo Wang; Bækkevold, Espen Sønderaal & Jahnsen, Frode Lars (2019). Resident memory CD8 T cells persist for years in human small intestine. Journal of Experimental Medicine.  ISSN 0022-1007.  216(10), s 2412- 2426 . doi: 10.1084/jem.20190414
  • Hung, Shu-Chen; Hou, Tieying; Jiang, Wei; Wang, Nan; Qiao, Shuo Wang; Chow, I-Ting; Liu, Xiaodan; van der Burg, Sjoerd H.; Koelle, David M; Kwok, William W; Sollid, Ludvig Magne & Mellins, Elizabeth D (2019). Epitope selection for HLA-DQ2 presentation: Implications for celiac disease and viral defense. Journal of Immunology.  ISSN 0022-1767.  202(9), s 2558- 2569 . doi: 10.4049/jimmunol.1801454
  • Høydahl, Lene Støkken; Richter, Lisa Maria; Frick, Rahel; Snir, Omri; Gunnarsen, Kristin Støen; Landsverk, Ole J. B.; Iversen, Rasmus; Jeliazkov, Jeliazko R.; Gray, Jeffrey J.; Bergseng, Elin; Foss, Stian; Qiao, Shuo Wang; Lundin, Knut Erik Aslaksen; Jahnsen, Jørgen; Jahnsen, Frode Lars; Sandlie, Inger; Sollid, Ludvig Magne & Løset, Geir Åge (2019). Plasma Cells Are the Most Abundant Gluten Peptide MHC-expressing Cells in Inflamed Intestinal Tissues From Patients With Celiac Disease. Gastroenterology.  ISSN 0016-5085.  156(5), s 1428- 1439.e10 . doi: 10.1053/j.gastro.2018.12.013
  • Dahal-Koirala, Shiva; Ciacchi, Laura; Petersen, Jan; Risnes, Louise Fremgaard; Neumann, Ralf Stefan; Christophersen, Asbjørn; Lundin, Knut Erik Aslaksen; Reid, Hugh H.; Qiao, Shuo Wang; Rossjohn, Jamie & Sollid, Ludvig Magne (2018). Discriminative T-cell receptor recognition of highly homologous HLA-DQ2-bound gluten epitopes. Journal of Biological Chemistry.  ISSN 0021-9258.  294(3), s 941- 952 . doi: 10.1074/jbc.RA118.005736
  • Risnes, Louise Fremgaard; Christophersen, Asbjørn; Dahal-Koirala, Shiva; Neumann, Ralf Stefan; Sandve, Geir Kjetil; Sarna, Vikas Kumar; Lundin, Knut Erik Aslaksen; Qiao, Shuo Wang & Sollid, Ludvig Magne (2018). Disease-driving CD4+ T cell clonotypes persist for decades in celiac disease. Journal of Clinical Investigation.  ISSN 0021-9738.  128(6), s 2642- 2650 . doi: 10.1172/JCI98819 Fulltekst i vitenarkiv.
  • Yao, Ying; Zia, Asima; Wyrozemski, Lukasz Adam; Lindeman, Ida; Sandve, Geir Kjetil & Qiao, Shuo Wang (2018). Exploiting antigen receptor information to quantify index switching in single-cell transcriptome sequencing experiments. PLOS ONE.  ISSN 1932-6203.  13(12), s 1- 17 . doi: 10.1371/journal.pone.0208484
  • Gunnarsen, Kristin Støen; Høydahl, Lene Støkken; Risnes, Louise Fremgaard; Dahal-Koirala, Shiva; Neumann, Ralf Stefan; Bergseng, Elin; Frigstad, Terje; Frick, Rahel; du Pré, Marie Fleur; Dalhus, Bjørn; Lundin, Knut Erik Aslaksen; Qiao, Shuo Wang; Sollid, Ludvig Magne; Sandlie, Inger & Løset, Geir Åge (2017). A TCRα framework–centered codon shapes a biased T cell repertoire through direct MHC and CDR3β interactions. JCI Insight.  ISSN 2379-3708.  2(17) . doi: 10.1172/jci.insight.95193 Fulltekst i vitenarkiv.
  • Raki, Melinda; Dahal-Koirala, Shiva; Yu, Hao; Korponay-Szabó, Ilma R.; Gyimesi, Judit; Castillejo, Gemma; Jahnsen, Jørgen; Qiao, Shuo Wang & Sollid, Ludvig Magne (2017). Similar Responses of Intestinal T Cells From Untreated Children and Adults With Celiac Disease to Deamidated Gluten Epitopes. Gastroenterology.  ISSN 0016-5085.  153(3), s 787- 798.e4 . doi: 10.1053/j.gastro.2017.05.016
  • Sarna, Vikas Kumar; Lundin, Knut Erik Aslaksen; Mørkrid, Lars; Qiao, Shuo Wang; Sollid, Ludvig Magne & Christophersen, Asbjørn (2017). HLA-DQ-gluten tetramer blood test accurately identifies patients with and without celiac disease in absence of gluten consumption. Gastroenterology.  ISSN 0016-5085.  154(4), s 886- 896.e6 . doi: 10.1053/j.gastro.2017.11.006 Fulltekst i vitenarkiv.
  • Christophersen, Asbjørn; Risnes, Louise Fremgaard; Bergseng, Elin; Lundin, Knut Erik Aslaksen; Sollid, Ludvig Magne & Qiao, Shuo Wang (2016). Healthy HLA-DQ2.5<sup>+</sup> subjects lack regulatory and memory T cells specific for immunodominant gluten epitopes of celiac disease. Journal of Immunology.  ISSN 0022-1767.  196(6), s 2819- 2826 . doi: 10.4049/jimmunol.1501152
  • Dahal-Koirala, Shiva; Risnes, Louise Fremgaard; Christophersen, Asbjørn; Sarna, Vikas Kumar; Lundin, Knut Erik Aslaksen; Sollid, Ludvig Magne & Qiao, Shuo Wang (2016). TCR sequencing of single cells reactive to DQ2.5-glia-α2 and DQ2.5-glia-ω2 reveals clonal expansion and epitope-specific V-gene usage. Mucosal Immunology.  ISSN 1933-0219.  9(3), s 587- 596 . doi: 10.1038/mi.2015.147 Fulltekst i vitenarkiv.
  • Lundin, Knut E A; Qiao, Shuo Wang; Snir, Omri & Sollid, Ludvig Magne (2015). Coeliac disease-from genetic and immunological studies to clinical applications. Scandinavian Journal of Gastroenterology.  ISSN 0036-5521.  50(6), s 708- 717 . doi: 10.3109/00365521.2015.1030766
  • Rath, Timo; Baker, Kristi; Dumont, Jennifer A.; Peters, Robert T.; Jiang, Haiyan; Qiao, Shuo Wang; Lencer, Wayne I.; Pierce, Glenn F. & Blumberg, Richard S. (2015). Fc-fusion proteins and FcRn: Structural insights for longer-lasting and more effective therapeutics. Critical Reviews in Biotechnology.  ISSN 0738-8551.  35(2), s 235- 254 . doi: 10.3109/07388551.2013.834293
  • Sollid, Ludvig Magne; Iversen, Rasmus; Steinsbø, Øyvind; Qiao, Shuo Wang; Bergseng, Elin; Dørum, Siri; du Pré, Fleur; Stamnæs, Jorunn; Christophersen, Asbjørn; Cardoso, Ines Do Rosario; Hnida, Kathrin; Xi, Chien; Snir, Omri & Lundin, Knut E A (2015). Small bowel, celiac disease and adaptive immunity. Digestive Diseases.  ISSN 0257-2753.  33(2), s 115- 121 . doi: 10.1159/000369512
  • Christophersen, Asbjørn; Raki, Melinda; Bergseng, Elin; Lundin, Knut E A; Jahnsen, Jørgen; Sollid, Ludvig Magne & Qiao, Shuo Wang (2014). Tetramer-visualized gluten-specific CD4+ T cells in blood as a potential diagnostic marker for coeliac disease without oral gluten challenge. United European Gastroenterology journal.  ISSN 2050-6406.  2(4), s 268- 278 . doi: 10.1177/2050640614540154
  • Dørum, Siri; Bodd, Michael; Fallang, Lars-Egil; Bergseng, Elin; Christophersen, Asbjørn; Johansen, Marie Kongshaug; Qiao, Shuo Wang; Stamnæs, Jorunn; De Souza, Gustavo Antonio & Sollid, Ludvig Magne (2014). HLA-DQ molecules as affinity matrix for identification of gluten T cell epitopes. Journal of Immunology.  ISSN 0022-1767.  193(9), s 4497- 4506 . doi: 10.4049/jimmunol.1301466
  • Qiao, Shuo Wang; Christophersen, Asbjørn; Lundin, Knut E A & Sollid, Ludvig Magne (2014). Biased usage and preferred pairing of α-and β-chains of TCRs specific for an immunodominant gluten epitope in coeliac disease. International Immunology.  ISSN 0953-8178.  26(1), s 13- 19 . doi: 10.1093/intimm/dxt037
  • Rath, Timo; Kuo, Timothy T.; Baker, Kristi; Qiao, Shuo Wang; Kobayashi, Kanna; Yoshida, Masaru; Roopenian, Derry C; Fiebiger, Edda; Lencer, Wayne I. & Blumberg, Richard S. (2013). The Immunologic Functions of the Neonatal Fc Receptor for IgG. Journal of Clinical Immunology.  ISSN 0271-9142.  33, s S9- S17 . doi: 10.1007/s10875-012-9768-y
  • Di Niro, Roberto; Mesin, Luka; Zheng, Nai-Ying; Stamnæs, Jorunn; Morrissey, Michael; Lee, Jane-Hwei; Huang, Min; Iversen, Rasmus; du Pré, Fleur; Qiao, Shuo Wang; Lundin, Knut E A; Wilson, Patric C. & Sollid, Ludvig Magne (2012). High abundance of plasma cells secreting transglutaminase 2-specific IgA autoantibodies with limited somatic hypermutation in celiac disease intestinal lesions. Nature Medicine.  ISSN 1078-8956.  18(3), s 441- U204 . doi: 10.1038/nm.2656
  • Qiao, Shuo Wang; Iversen, Rasmus; Raki, Melinda & Sollid, Ludvig Magne (2012). The adaptive immune response in celiac disease. Springer Seminars in Immunopathology.  ISSN 0344-4325.  34(4), s 523- 540 . doi: 10.1007/s00281-012-0314-z
  • Sollid, Ludvig Magne; Qiao, Shuo Wang; Anderson, Robert P; Gianfrani, Carmen & Koning, Frits (2012). Nomenclature and listing of celiac disease relevant gluten T-cell epitopes restricted by HLA-DQ molecules. Immunogenetics.  ISSN 0093-7711.  64(6), s 455- 460 . doi: 10.1007/s00251-012-0599-z
  • Baker, Kristi; Qiao, Shuo Wang; Kuo, Timothy T.; Aveson, Victoria G.; Platzer, Barbara; Andersen, Jan Terje; Sandlie, Inger; Chen, Zhangguo; de Haar, Colin; Lencer, Wayne I.; Fiebiger, Edda & Blumberg, Richard S. (2011). Neonatal Fc receptor for IgG (FcRn) regulates cross-presentation of IgG immune complexes by CD8(-)CD11b(+) dendritic cells. Proceedings of the National Academy of Sciences of the United States of America.  ISSN 0027-8424.  108(24), s 9927- 9932 . doi: 10.1073/pnas.1019037108
  • Qiao, Shuo Wang; Raki, Melinda; Gunnarsen, Kristin Støen; Løset, Geir Åge; Lundin, Knut E A; Sandlie, Inger & Sollid, Ludvig Magne (2011). Posttranslational Modification of Gluten Shapes TCR Usage in Celiac Disease. Journal of Immunology.  ISSN 0022-1767.  187(6), s 3064- 3071 . doi: 10.4049/jimmunol.1101526
  • Rauhavirta, T.; Qiao, Shuo Wang; Jiang, Z.; Myrsky, E.; Loponen, J.; Korponay-Szabo, I.R.; Salovaara, H.; Garcia-Horsman, J.A.; Venäläinen, J.; Männistö, P.T.; Collighan, R.; Mongeot, A.; Griffin, M.; Mäki, M; Kaukinen, K & Lindfors, K. (2011). Epithelial transport and deamidation of gliadin peptides: a role for coeliac disease patient immunoglobulin A. Clinical and Experimental Immunology.  ISSN 0009-9104.  164(1), s 127- 136 . doi: 10.1111/j.1365-2249.2010.04317.x
  • Dørum, Siri; Arntzen, Magnus; Qiao, Shuo Wang; Holm, Anders; Koehler, Christian; Thiede, Bernd; Sollid, Ludvig Magne & Fleckenstein, Burkhard (2010). The Preferred Substrates for Transglutaminase 2 in a Complex Wheat Gluten Digest Are Peptide Fragments Harboring Celiac Disease T-Cell Epitopes. PLOS ONE.  ISSN 1932-6203.  5(11) . doi: 10.1371/journal.pone.0014056 Vis sammendrag
  • Kuo, T; Baker, K; Yoshida, M; Qiao, Shuo Wang; Aveson, VG; Lencer, WI & Blumberg, RS (2010). Neonatal Fc Receptor: From Immunity to Therapeutics. Journal of Clinical Immunology.  ISSN 0271-9142.  30(6), s 777- 789 . doi: 10.1007/s10875-010-9468-4
  • Baker, K; Qiao, Shuo Wang; Kuo, T; Kobayashi, K; Yoshida, M; Lencer, WI & Blumberg, RS (2009). Immune and non-immune functions of the (not so) neonatal Fc receptor, FcRn. Springer Seminars in Immunopathology.  ISSN 0344-4325.  31(2), s 223- 236 . doi: 10.1007/s00281-009-0160-9
  • Dørum, Siri; Qiao, Shuo Wang; Sollid, Ludvig Magne & Fleckenstein, Burkhard (2009). A Quantitative Analysis of Transglutaminase 2-Mediated Deamidation of Gluten Peptides: Implications for the T-cell Response in Celiac Disease. Journal of Proteome Research.  ISSN 1535-3893.  8(4), s 1748- 1755 . doi: 10.1021/pr800960n
  • Kobayashi, Kanna; Qiao, Shuo Wang; Yoshida, Masaru; Baker, Kristi; Lencer, Wayne I. & Blumberg, Richard S. (2009). An FcRn-Dependent Role for Anti-flagellin Immunoglobulin G in Pathogenesis of Colitis in Mice. Gastroenterology.  ISSN 0016-5085.  137(5), s 1746- 1756 . doi: 10.1053/j.gastro.2009.07.059
  • Qiao, Shuo Wang; Sollid, Ludvig Magne & Blumberg, Richard S (2009). Antigen presentation in celiac disease. Current Opinion in Immunology.  ISSN 0952-7915.  21(1), s 111- 117 . doi: 10.1016/j.coi.2009.03.004
  • Stenman, SM; Venalainen, JI; Lindfors, K.; Auriola, S; Mauriala, T; Kaukovirta-Norja, A; Jantunen, A; Laurila, K; Qiao, Shuo Wang; Sollid, Ludvig Magne; Mannisto, Pekka T; Kaukinen, K & Mäki, M (2009). Enzymatic detoxification of gluten by germinating wheat proteases: Implications for new treatment of celiac disease. Annals of Medicine.  ISSN 0785-3890.  41(5), s 390- 400 . doi: 10.1080/07853890902878138
  • Qiao, Shuo Wang; Kobayashi, K; Johansen, Finn Eirik; Sollid, Ludvig Magne; Andersen, Jan Terje; Milford, E; Roopenian, DC; Lencer, WI & Blumberg, RS (2008). Dependence of antibody-mediated presentation of antigen on FcRn. Proceedings of the National Academy of Sciences of the United States of America.  ISSN 0027-8424.  105, s 9337- 9342 . doi: 10.1073/pnas.0801717105
  • Qiao, Shuo-Wang; Bergseng, Elin; Molberg, Øyvind; Jung, G; Fleckenstein, Burkhard & Sollid, Ludvig Magne (2005). Refining the rules of gliadin T cell epitope binding to the disease-associated DQ2 molecule in celiac disease: Importance of proline spacing and glutamine deamidation. Journal of Immunology.  ISSN 0022-1767.  175(1), s 254- 261
  • Qiao, Shuo-Wang; Piper, J; Haraldsen, Guttorm; Øynebråten, Inger; Fleckenstein, Burkhard; Molberg, Øyvind; Khosla, C & Sollid, Ludvig Magne (2005). Tissue transglutaminase-mediated formation and cleavage of histamine-gliadin complexes: biological effects and implications for celiac disease. Journal of Immunology.  ISSN 0022-1767.  174, s 1657- 1663
  • Shan, L; Qiao, Shuo Wang; Arentz-Hansen, Eva Helene; Molberg, Øyvind; Gray, G.M.; Sollid, Ludvig Magne & Khosla, C. (2005). Identification and Analysis of Multivalent Proteolytically Resistant Peptides from Gluten: Implications for Celiac Sprue. Journal of Proteome Research.  ISSN 1535-3893.  4, s 1732- 1741
  • Fleckenstein, Burkhard; Qiao, Shuo-Wang; Larsen, MR; Jung, G; Roepstorff, P. & Sollid, Ludvig Magne (2004). Molecular characterization of covalent complexes between tissue transglutaminase and gliadin peptides. Journal of Biological Chemistry.  ISSN 0021-9258.  279, s 17607- 17616
  • Qiao, Shuo-Wang; Bergseng, Elin; Molberg, Øyvind; Xia, J; Fleckenstein, Burkhard; Khosla, C & Sollid, Ludvig Magne (2004). Antigen presentation to celiac lesion-derived T cells of a 33-mer gliadin peptide naturally formed by gastrointestinal digestion. Journal of Immunology.  ISSN 0022-1767.  173(3), s 1757- 1762
  • Fleckenstein, Burkhard; Molberg, Øyvind; Qiao, Shuo Wang; Schmid, Dietmar G.; Mülbe, Florian von der; Elgstoen, Katja Benedikte Prestø; Jung, Günter & Sollid, Ludvig Magne (2002). Gliadin T Cell Epitope Selection by Tissue Transglutaminase in Celiac Disease. Role of Enzyme Specificity and pH Influence on the Transamidation Versus Deamidation Reactions. Journal of Biological Chemistry.  ISSN 0021-9258.  277, s 34109- 34116
  • Boix, Fernando; Qiao, Shuo Wang; Kolpus, Tone & Sagvolden, Terje (1998). Chronic L-deprenyl treatment alters brain monoamine levels and reduces impulsiveness in an animal model of Attention-Deficit/Hyperactivity Disorder. Behavioural Brain Research.  ISSN 0166-4328.  94(1), s 153- 162 Vis sammendrag

Se alle arbeider i Cristin

  • Lindeman, Ida; Emerton, Guy; Mamanova, Lira; Snir, Omri; Polanski, Krzysztof; Qiao, Shuo Wang; Sollid, Ludvig Magne; Teichmann, Sarah A & Stubbington, Michael J. T. (2018). BraCeR: B-cell-receptor reconstruction and clonality inference from single-cell RNA-seq. Nature Methods.  ISSN 1548-7091.  15(8), s 563- 565 . doi: 10.1038/s41592-018-0082-3
  • Hou, Tieying; Qiao, Shuo Wang; Jin, Xi; Sidney, John; Sollid, Ludvig Magne; Macmillan, Henriette; Strohman, Michael; Yoon, Taejin & Mellins, Elizabeth (2012). Interaction of HLA-DM and HLA-DQ2 in antigen presentation: Implications for celiac disease association. Molecular Immunology.  ISSN 0161-5890.  51(1), s 40- 40 . doi: 10.1016/j.molimm.2012.03.005
  • Hou, Tieying; Qiao, Shuo Wang; Jin, Xi; Sidney, John; Sollid, Ludvig Magne; Macmillan, Henriette; Strohman, Michael; Yoon, Taejin & Mellins, Elizabeth (2012). Interaction of HLA-DM and HLA-DQ2 in antigen presentation: Implications for celiac disease association. Molecular Immunology.  ISSN 0161-5890.  51(1), s 38- 38 . doi: 10.1016/j.molimm.2012.02.115
  • Hou, Tieying; Qiao, Shuo Wang; Jin, Xi; Sidney, John; Sollid, Ludvig Magne & Mellins, Elizabeth (2012). Cryptic Fate of Gliadin Epitopes: Implication for Celiac Disease. Journal of Immunology.  ISSN 0022-1767.  188
  • Høydahl, Lene Støkken; Neumann, Ralf Stefan; Qiao, Shuo Wang; Sollid, Ludvig Magne; Sandlie, Inger & Løset, Geir Åge (2011). Development of a High Affinity TCR Reagent by Phage Display to Study Antigen Presentation in Celiac Disease. Scandinavian Journal of Immunology.  ISSN 0300-9475.  73(4), s 372- 372
  • Qiao, Shuo Wang; Raki, Melinda; Gunnarsen, Kristin Støen; Løset, Geir Åge; Sandlie, Inger & Sollid, Ludvig Magne (2011). Post-Translational Modification of Gluten Shapes the T Cell Response in Coeliac Disease by Direct Interaction with the T Cell Receptor. Scandinavian Journal of Immunology.  ISSN 0300-9475.  73(4), s 356- 356
  • Qiao, Shuo Wang; Raki, Melinda; Løset, Geir Åge; Sandlie, Inger & Sollid, Ludvig Magne (2010). TCR Vbeta Bias and Semi-Public T Cell Responses In DQ2-Gliadin-Alpha-II Specific T Cells From Coeliac Disease Patients. Scandinavian Journal of Immunology.  ISSN 0300-9475.  71(6), s 511- +

Se alle arbeider i Cristin

Publisert 13. apr. 2011 11:43 - Sist endret 24. juni 2019 11:56