Faglige interesser
- Proteaser i vaskulærbiologi
- Arterosklerose (forkalkning), slag og hjerteinfarkt
- Trombose (blodpropp) og koagulasjon
- Inflammasjon
Undervisning
- Medisinsk biokjemi
- Hovedveileder for doktorgradstudenter Kristina Byskov, Nis Valentin Nielsen, Emrah Kara, Simona Grasso og Sai Priya Sarma Kandanur.
Bakgrunn
- Habilitation, University of Giessen, Tyskland, 2003
- PhD, University of London, Storbritannia, 1986-1991
- BSc Biochemistry, University of London, Storbritannia 1983-1986
Arbeidserfaring
- Leder av seksjon Biokjemi siden 2018
- Member of the Excellence Cluster in Cardiopulmonary Sciences (ECCPS), University of Giessen, Germany (2007-2014)
- Member of the Sonderforschungsbereich SFB-547 University of Giessen, Germany (2006-2009)
- Faculty Member, University of Giessen, Germany, 1999-2011
- Postdoctoral Fellow, Max Planck Institute, Bad Nauheim, Germany, 1994-1998
- Postdoctoral Fellow, Thrombosis Research Institute, London, UK 1991-1993
Emneord:
Hjerte- og karsykdom,
Hjerneslag,
Trombose,
Aterosklerose,
Koagulasjon,
Fibrinolyse,
Proteaser,
Blod og immunologi,
Inflammasjon
Publikasjoner
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Harjen, Hannah Jayne; Hellum, Marit Synnøve; Rørtveit, Runa; Oscarson, Malin; Anfinsen, Kristin Paaske & Moldal, Elena Regine
[Vis alle 9 forfattere av denne artikkelen]
(2022).
Persistent hypercoagulability in dogs envenomated by the European adder (Vipera berus berus).
PLOS ONE.
ISSN 1932-6203.
17(2).
doi:
10.1371/journal.pone.0263238.
Fulltekst i vitenarkiv
Vis sammendrag
Background
Envenomation by the European adder, Vipera berus berus (Vbb), is a medical emergency. The overall in vivo haemostatic effects of pro- and anticoagulant components in Vbb venom, and the downstream effects of cellular injury and systemic inflammation, are unclear.
Objectives
To longitudinally describe the global coagulation status of dogs after Vbb envenomation and compare to healthy controls. A secondary aim was to investigate differences between dogs treated with and without antivenom.
Methods
Citrated plasma was collected at presentation, 12 hours (h), 24 h, 36 h and 15 days after bite from 28 dogs envenomated by Vbb, and from 28 healthy controls at a single timepoint. Thrombin generation (initiated with and without exogenous phospholipids and tissue factor), thrombin-antithrombin (TAT)-complexes and the procoagulant activity of phosphatidylserine (PS)-expressing extracellular vesicles (EVs), expressed as PS-equivalents, were measured.
Results
At presentation the envenomated dogs were hypercoagulable compared to controls, measured as increased thrombin generation, TAT-complexes and PS-equivalents. The hypercoagulability decreased gradually but compared to controls thrombin generation and PS-equivalents were still increased at day 15. The discrepancy in peak thrombin between envenomated dogs and controls was greater when the measurement was phospholipid-dependent, indicating that PS-positive EVs contribute to hypercoagulability. Lag time was shorter in non-antivenom treated dogs, compared to antivenom treated dogs <24 h after envenomation.
Conclusions
Hypercoagulability was measured in dogs up to 15 days after Vbb envenomation. Dogs treated with antivenom may be less hypercoagulable than their non-antivenom treated counterparts. Thrombin generation is a promising diagnostic and monitoring tool for Vbb envenomation.
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Gramstad, Olav Rogde; Kandanur, Sai Priya Sarma; Etscheid, Michael; Nielsen, Erik Waage & Kanse, Sandip
(2021).
Factor VII activating protease (FSAP) is not essential in the pathophysiology of angioedema in patients with C1 inhibitor deficiency.
Molecular Immunology.
ISSN 0161-5890.
142,
s. 95–104.
doi:
10.1016/j.molimm.2021.11.019.
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Kara, Emrah; Nielsen, Nis Valentin; Eggertsdottir, Bergrun; Thiede, Bernd; Kanse, Sandip & Løset, Geir Åge
(2020).
Design and characterization of a new pVII combinatorial phage display peptide library for protease substrate mining using Factor VII activating protease (FSAP) as model.
ChemBioChem.
ISSN 1439-4227.
21(13),
s. 1875–1884.
doi:
10.1002/cbic.201900705.
Fulltekst i vitenarkiv
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Byskov, Kristina; Etscheid, Michael & Kanse, Sandip
(2020).
Cellular effects of factor VII activating protease (FSAP).
Thrombosis Research.
ISSN 0049-3848.
188,
s. 74–78.
doi:
10.1016/j.thromres.2020.02.010.
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Factor VII activating protease (FSAP) is a circulating serine protease of broad specificity that is likely to be involved in many pathophysiological processes. The activation of the circulating zymogen form of FSAP by histones, released from damaged cells, underlines its roles in regulating host responses to tissue damage and inflammation. Some of the direct cellular effects of FSAP are mediated through protease-activated receptors (PARs). Knock-down of each one of the four PARs in endothelial cells indicated that PAR-1 and -3 are involved in regulating endothelial permeability in response to FSAP. Overexpression of PARs in cell lines led to the conclusion that PAR-2 and -1 were the main receptors for FSAP. Studies with synthetic peptides and receptor mutants demonstrate that FSAP cleaves PAR-1 and -2 at their canonical cleavage site. However, PAR-1 is not activated by FSAP in all cells, which may be related to other, as yet, undefined factors. Inhibition of apoptosis by FSAP is mediated through PAR-1 and was observed in neurons, astrocytes and A549 cells. FSAP also mediates cellular effects by modulating the activity of growth factors, generation of bradykinin, C5a and C3a generation or histone inactivation. These cellular effects need to be further investigated at the in vivo level.
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Byskov, Kristina; M. le Gall, Sylvain; Thiede, Bernd; Camerer, Eric & Kanse, Sandip
(2019).
Protease activated receptors (PAR)‐1 and ‐2 mediate cellular effects of factor VII activating protease (FSAP).
The FASEB Journal.
ISSN 0892-6638.
34,
s. 1079–1090.
doi:
10.1096/fj.201801986RR.
Fulltekst i vitenarkiv
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Cole, John W.; Kanse, Sandip; Authors, Other & Mitchell, Braxton
(2018).
Genetics of the thrombomodulin-endothelial
cell protein C receptor system and the risk of
early-onset ischemic stroke.
PLOS ONE.
ISSN 1932-6203.
doi:
10.1371/journal.pone.0206554.
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Subramaniam, Saravanan; Kanse, Sandip; Kothari, Hema; Reinhardt, Christoph & Craig, Fletcher
(2018).
Post-transcriptional, post-translational and pharmacological
regulation of tissue factor pathway inhibitor.
Blood Coagulation and Fibrinolysis.
ISSN 0957-5235.
29(8),
s. 668–682.
doi:
10.1097/MBC.0000000000000775.
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Praetner, M; Zuchtriegel, G; Holzer, M; Uhl, B; Schaubaecher, J & Mittmann, L
[Vis alle 16 forfattere av denne artikkelen]
(2018).
Plasminogen Activator Inhibitor-1 Promotes Neutrophil Infiltration and Tissue Injury on Ischemia-Reperfusion.
Arteriosclerosis, Thrombosis and Vascular Biology.
ISSN 1079-5642.
38(4),
s. 829–842.
doi:
10.1161/ATVBAHA.117.309760.
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Herold, Joerg; Nowak, Steven; Kostin, Sawa; Daniel, Jan-Marcus; Francke, Alexander & Subramaniam, Saravanan
[Vis alle 8 forfattere av denne artikkelen]
(2017).
Factor VII activating protease (FSAP) influences vascular remodeling in the mouse hind limb ischemia model.
American journal of translational research.
ISSN 1943-8141.
9(6),
s. 3084–3095.
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Espada Serrano, Sandra; Stavik, Benedicte; Holm, Sverre; Sagen, Ellen Lund; Bjerkeli, Vigdis & Skjelland, Mona
[Vis alle 12 forfattere av denne artikkelen]
(2017).
Tissue factor pathway inhibitor attenuates ER stress-induced inflammation in human M2-polarized macrophages.
Biochemical and Biophysical Research Communications - BBRC.
ISSN 0006-291X.
491(2),
s. 442–448.
doi:
10.1016/j.bbrc.2017.07.070.
Vis sammendrag
Endoplasmic reticulum (ER) stress has been shown to play a key role during the initiation and clinical progression of the cardiovascular diseases, such as atherosclerosis. We have recently shown that expression of tissue factor pathway inhibitor (TFPI) in human monocyte-derived macrophages (MDMs) was induced by cholesterol crystals (CC). In the present study we aimed to determine the role of TFPI under ER stress conditions using human MDMs. qRT-PCR and immunohistochemistry analysis were performed to determine the presence of the ER stress marker CCAAT/enhancer binding protein homologous protein (CHOP) and TFPI in human carotid plaque material and also in human MDMs polarized into pro-inflammatory M1 or anti-inflammatory M2 populations. CHOP mRNA levels were upregulated in the plaques compared to healthy vessels, and CHOP protein was localized in the same area as TFPI in the plaques. Both CHOP and TFPI mRNA levels were upregulated after CC treatment, especially in the M2 phenotype, and the ER stress inhibitor 4-phenylbutyric acid (PBA) reversed this effect. Furthermore, CC treatment increased the levels of the pro-inflammatory cytokines TNF-α, IL-6, and IL-8, which for TNF-α and IL-8 was inhibited by PBA, and reduced the levels of the anti-inflammatory cytokine IL-10 in M2-polarized macrophages. Knockdown of TFPI prior to CC treatment exacerbated TNF-α and IL-6 levels, but reduced IL-8 and IL-10 levels. Our results show that CC induce TFPI and cytokine expression in M2-polarized macrophages through activation of the ER stress pathway and that TFPI has a protective effect against TNF-α and IL-6 mediated inflammation. These mechanisms may have implications for the pathogenesis of atherosclerosis.
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Kara, Emrah; Manna, Dipankar; Løset, Geir Åge; Schneider, E L; Craik, C S & Kanse, Sandip
(2017).
Analysis of the substrate specificity of Factor VII activating protease (FSAP) and design of specific and sensitive peptide substrates.
Thrombosis and Haemostasis.
ISSN 0340-6245.
doi:
10.1160/TH17-02-0081.
Fulltekst i vitenarkiv
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Bustamante, Alejandro; Diaz-Fernandez, Belen; Giralt, Dolors; Boned, Sandra; Pagola, Jorge & Molina, Carlos
[Vis alle 9 forfattere av denne artikkelen]
(2016).
Factor seven activating protease (FSAP) predicts response to intravenous thrombolysis in acute ischemic stroke.
International Journal of Stroke.
ISSN 1747-4930.
11(6),
s. 646–655.
doi:
10.1177/1747493016641949.
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Leiting, Silke; Seidl, Sebastian; Martinez-Palaciàn, Adoraciòn; Muhl, Lars & Kanse, Sandip
(2016).
Transforming growth factor-β (TGF-β) inhibits the expression of factor VII-activating protease (FSAP) in hepatocytes.
Journal of Biological Chemistry.
ISSN 0021-9258.
291(40),
s. 21020–21028.
doi:
10.1074/jbc.M116.744631.
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Reichel, Christoph A.; Hessenauer, Maximilian E.T.; Pflieger, Kerstin; Rehberg, Markus; Kanse, Sandip & Zahler, Stefan
[Vis alle 9 forfattere av denne artikkelen]
(2015).
Components of the plasminogen activation system promote engraftment of porous polyethylene biomaterial via common and distinct effects.
PLOS ONE.
ISSN 1932-6203.
10:e0116883(2).
doi:
10.1371/journal.pone.0116883.
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Joshi, Amit; Orset, Cyrille; Engelhardt, Britta; Baumgart-Vogt, Eveline; Gerriets, Tibo & Vivien, Denis
[Vis alle 7 forfattere av denne artikkelen]
(2015).
Deficiency of Factor VII Activating Protease (FSAP) alters the outcome of ischemic stroke in mice.
European Journal of Neuroscience.
ISSN 0953-816X.
doi:
10.1111/ejn.12830.
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Subramaniam, Saravanan; Thielmann, Ina; Morowski, Martina; Pragst, Ingo; Sandset, Per Morten & Nieswandt, Bernhard
[Vis alle 8 forfattere av denne artikkelen]
(2015).
Defective thrombus formation in mice lacking endogenous factor VII activating protease (FSAP).
Thrombosis and Haemostasis.
ISSN 0340-6245.
113(4),
s. 870–880.
doi:
10.1160/TH14-06-0519.
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Subramaniam, Saravanan & Kanse, Sandip
(2014).
Ferric Chloride–Induced Arterial Thrombosis in Mice.
Current Protocols in Mouse Biology.
ISSN 2161-2617.
doi:
10.1002/9780470942390.mo140140.
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Martinez-Palaciàn, Adoraciòn; Kanse, Sandip & Weiskirchen, Ralf
(2014).
Factor VII activating protease (FSAP): A novel protective factor in liver fibrosis.
PROTEOMICS - Clinical Applications.
ISSN 1862-8346.
8(5-6),
s. 438–446.
doi:
10.1002/prca.201300078.
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Parahuleva, Mariana S.; Kanse, Sandip; Hölschermann, Hans; Zheleva, Kirila; Zandt, Daniel & Worsch, Michael
[Vis alle 11 forfattere av denne artikkelen]
(2014).
Association of circulating factor seven activating protease (FSAP) and of oral Omega-3 fatty acids supplements with clinical outcome in patients with atrial fibrillation: the OMEGA-AF study.
Journal of Thrombosis and Thrombolysis.
ISSN 0929-5305.
37(3),
s. 317–325.
doi:
10.1007/s11239-013-0921-0.
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Roedel, Elfie Kathrin; Schwarz, Elisabeth & Kanse, Sandip
(2013).
The Factor VII-activating Protease (FSAP) Enhances the Activity of Bone Morphogenetic Protein-2 (BMP-2).
Journal of Biological Chemistry.
ISSN 0021-9258.
288(10),
s. 7193–7203.
doi:
10.1074/jbc.M112.433029.
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Etscheid, Michael; Muhl, L.; Pons, D.; Jukema, J. Wouter; Konig, H. & Kanse, Sandip
(2012).
The Marburg I polymorphism of factor VII activating protease is associated with low proteolytic and low pro-coagulant activity.
Thrombosis Research.
ISSN 0049-3848.
130(6),
s. 935–941.
doi:
10.1016/j.thromres.2012.07.023.
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Fraineau, Sylvain; Monvoisin, Arnaud; Clarhaut, Jonathan; Talbot, Julie; Simonneau, Claire & Kanthou, Chryso
[Vis alle 9 forfattere av denne artikkelen]
(2012).
The vitamin K-dependent anti-coagulant factor, protein S, inhibits multiple VEGF-A-induced angiogenesis events in a Mer- and SHP2-dependent manner.
Blood.
ISSN 0006-4971.
120(25),
s. 5073–5083.
doi:
10.1182/blood-2012-05-429183.
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Parahuleva, Mariana S; Hölschermann, Hans; Zandt, Daniel; Pons-Kühnemann, Jörn; Parviz, Behnoush & Weiskirchen, Ralf
[Vis alle 10 forfattere av denne artikkelen]
(2012).
Circulating Factor VII Activating Protease (FSAP) Is Associated With Clinical Outcome in Acute Coronary Syndrome.
Circulation Journal.
ISSN 1346-9843.
76(11),
s. 2653–2661.
doi:
10.1253/circj.CJ-11-1502.
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Parahuleva, Mariana S; Langanke, Eva; Hölschermann, Hans; Parviz, Behnoush; Abdallah, Yaser & Stracke, Siegbert
[Vis alle 8 forfattere av denne artikkelen]
(2012).
Nicotine Modulation of Factor VII Activating Protease (FSAP) Expression in Human Monocytes.
Journal of atherosclerosis and thrombosis.
ISSN 1880-3873.
19(11),
s. 962–969.
doi:
10.5551/jat.9589.
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Reichel, Christoph A.; Kanse, Sandip & Krombach, Fritz
(2012).
At the Interface of Fibrinolysis and Inflammation: The Role of Urokinase-Type Plasminogen Activator in the Leukocyte Extravasation Cascade.
Trends in cardiovascular medicine.
ISSN 1050-1738.
22(7),
s. 192–196.
doi:
10.1016/j.tcm.2012.07.019.
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Kanse, Sandip; Declerck, Paul J.; Ruf, Wolfram; Broze, George & Etscheid, Michael
(2012).
Factor VII-Activating Protease Promotes the Proteolysis and Inhibition of Tissue Factor Pathway Inhibitor.
Arteriosclerosis, Thrombosis and Vascular Biology.
ISSN 1079-5642.
32(2),
s. 427–U619.
doi:
10.1161/ATVBAHA.111.238394.
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Kanse, Sandip; Gallenmueller, Andrea; Zeerleder, Sacha; Stephan, Femke; Rannou, Olivier & Denk, Stephanie
[Vis alle 10 forfattere av denne artikkelen]
(2012).
Factor VII-Activating Protease Is Activated in Multiple Trauma Patients and Generates Anaphylatoxin C5a.
Journal of Immunology.
ISSN 0022-1767.
188(6),
s. 2858–2865.
doi:
10.4049/jimmunol.1103029.
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Kanse, Sandip; Gallenmueller, Andrea; Zeerleder, Sasha; Stephan, Femke; Rannou, Olivier & Denk, Stephanie
[Vis alle 10 forfattere av denne artikkelen]
(2012).
Factor VII activating protease (FSAP) is massively activated in multiple trauma patients and generates anaphylatoxin C5a.
Journal of Immunology.
ISSN 0022-1767.
188(6),
s. 2858–2865.
doi:
10.4049/jimmunol.1103029.
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Kanse, Sandip; Declerk, Paul J; Ruf, Wolfram; Broze, George & Etscheid, Michael
(2012).
Factor VII activating protease (FSAP) promotes the proteolysis and inhibition of tissue factor pathway inhibitor.
Arteriosclerosis, Thrombosis and Vascular Biology.
ISSN 1079-5642.
32,
s. 427–433.
doi:
10.1161/atvbaha.111.238394.
Se alle arbeider i Cristin
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Bohnert, Bernhard; Kanse, Sandip; Haerteis, Silke; Korbmacher, Christoph & Artunc, Ferruh
(2019).
Rebuttal to Editorial: Sodium Retention by uPA in Nephrotic Syndrome?
Acta Physiologica.
ISSN 1748-1708.
doi:
10.1111/apha.13427.
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Kara, Emrah; Manna, Dipankar; Løset, Geir Åge; Schneider, Eric L.; Craik, Charles S. & Kanse, Sandip
(2017).
Analysis of the Substrate Specificity of Factor VII Activating Protease (FSAP) and Design of a Specific and Sensitive Peptide Substrate.
Res Pract Thromb Haemost.
s. 560–560.
doi:
10.1002/rth2.12012.
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Nielsen, Nis Valentin; Manna, Dipankar; J. Preben, Morth; Roedel, Elfie; Etscheid, Michael & Kanse, Sandip
(2017).
Factor VII Activating Protease (FSAP) and Marburg I (G534E) Polymorphism; How One Amino Acid Determines Function.
Res Pract Thromb Haemost.
s. 358–359.
doi:
10.1002/rth2.12012.
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Nielsen, Nis Valentin; Morth, Jens Preben & Kanse, Sandip
(2016).
Structure function analysis of Factor Seven Activating Protease (FSAP)
a protease in vascular biology.
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Kanse, Sandip
(2015).
Factor VII activating protease (FSAP):From single nucleotide polymorphisms to mechanisms of action in thrombosis and vascular diseases.
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Nielsen, Nis Valentin; Morth, Jens Preben & Kanse, Sandip
(2015).
Expression, Purification and Refolding of Factor Seven Activating Protease(FSAP) for structure function analysis.
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Nielsen, Nis Valentin; Morth, Jens Preben & Kanse, Sandip
(2015).
Purification and refolding of Factor Seven Activating Protease (FSAP) a protease in vascular biology.
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Nielsen, Nis Valentin; Morth, Jens Preben & Kanse, Sandip
(2015).
Purification and refolding of Factor Seven Activating Protease (FSAP) a protease in vascular biology.
Se alle arbeider i Cristin
Publisert
12. jan. 2012 14:36
- Sist endret
10. juli 2023 12:24