Nåværende stilling
- 2011 - d.d: Forsker, Kjetil Taskén Biotek Group (Cell Signalling), tilsatt på prosjektet “MARINE FUNGI - natural products from marine fungi for the treatment of cancer".
Bakgrunn
Forskning:
- 2008 - 2011: Postdoktor, Kjetil Taskén Biotek Group (Cell Signalling), Bioteknologisenteret i Oslo, tilsatt på et prosjekt finansiert av Norgens Forskningsråds satsing FUGE.
Utdanning:
- 2007: Doktorgrad (Dr. rer. nat.), Institutt for zoologi, universitet i Regensburg, Tyskland.
Doktoravhandlingen: “Studies on the clock proteins PERIOD and TIMELESS in the fruit fly Drosophila melanogaster.”
Emneord:
Cell signalling
Publikasjoner
-
Giesecke, Astrid; Johnstone, Peter S.; Lamaze, Angelique; Landskron, Johannes; Atay, Ezgi & Chen, Ko-Fan
[Vis alle 9 forfattere av denne artikkelen]
(2023).
A novel period mutation implicating nuclear export in temperature compensation of the Drosophila circadian clock.
Current Biology.
ISSN 0960-9822.
33(2),
s. 336–350.
doi:
10.1016/j.cub.2022.12.011.
-
-
Meås, Hany Zakaria; Haug, Markus; Beckwith, Marianne Sandvold; Louet, Claire; Ryan, Liv & Hu, Zhenyi
[Vis alle 12 forfattere av denne artikkelen]
(2020).
Sensing of HIV-1 by TLR8 activates human T cells and reverses latency.
Nature Communications.
ISSN 2041-1723.
11:147,
s. 1–16.
doi:
10.1038/s41467-019-13837-4.
Fulltekst i vitenarkiv
Vis sammendrag
During HIV infection, cell-to-cell transmission results in endosomal uptake of the virus by target CD4+ T cells and potential exposure of the viral ssRNA genome to endosomal Toll-like receptors (TLRs). TLRs are instrumental in activating inflammatory responses in innate immune cells, but their function in adaptive immune cells is less well understood. Here we show that synthetic ligands of TLR8 boosted T cell receptor signaling, resulting in increased cytokine production and upregulation of surface activation markers. Adjuvant TLR8 stimulation, but not TLR7 or TLR9, further promoted T helper cell differentiation towards Th1 and Th17. In addition, we found that endosomal HIV induced cytokine secretion from CD4+ T cells in a TLR8-specific manner. TLR8 engagement also enhanced HIV-1 replication and potentiated the reversal of latency in patient-derived T cells. The adjuvant TLR8 activity in T cells can contribute to viral dissemination in the lymph node and low-grade inflammation in HIV patients. In addition, it can potentially be exploited for therapeutic targeting and vaccine development.
-
-
-
-
Liu, Lisa L.; Landskron, Johannes; Ask, Eivind Heggernes; Enqvist, Monika; Sohlberg, Ebba & Traherne, James A.
[Vis alle 18 forfattere av denne artikkelen]
(2016).
Critical Role of CD2 Co-stimulation in Adaptive Natural Killer Cell Responses Revealed in NKG2C-Deficient Humans.
Cell reports.
ISSN 2211-1247.
15(3),
s. 1088–1099.
doi:
10.1016/j.celrep.2016.04.005.
-
-
-
Ellinger, Bernhard; Silber, Johanna; Prashar, Anjali; Landskron, Johannes; Weber, Jonas & Rehermann, Sarah
[Vis alle 13 forfattere av denne artikkelen]
(2014).
A phenotypic screening approach to identify anticancer compounds derived from marine fungi.
Assay and drug development technologies.
ISSN 1540-658X.
12(3),
s. 162–175.
doi:
10.1089/adt.2013.564.
-
-
-
-
Hagness, Morten; Henjum, Karen; Landskron, Johannes; Brudvik, Kristoffer Watten; Bjørnbeth, Bjørn Atle & Foss, Aksel
[Vis alle 8 forfattere av denne artikkelen]
(2012).
Kinetics and Activation Requirements of Contact-Dependent Immune Suppression by Human Regulatory T Cells.
Journal of Immunology.
ISSN 0022-1767.
188(11),
s. 5459–5466.
doi:
10.4049/jimmunol.1101367.
-
Eriksen, Agnete Bratsberg; Indrevær, Randi Larsen; Holm, Kristine Lillebø; Landskron, Johannes & Blomhoff, Heidi Kiil
(2012).
TLR9-signaling is required for turning retinoic acid into a potent stimulator of RP105 (CD180)-mediated proliferation and IgG synthesis in human memory B cells.
Cellular Immunology.
ISSN 0008-8749.
279(1),
s. 87–95.
doi:
10.1016/j.cellimm.2012.09.003.
-
-
Saunders, Threrese Solstad; Bains, Simer Jit; Landskron, Johannes; Aandahl, Einar Martin; Thiede, Bernd & Tasken, Kjetil
[Vis alle 7 forfattere av denne artikkelen]
(2011).
CD147 (Basigin/Emmprin) identifies FoxP3(+)CD45RO(+)CTLA4(+)-activated human regulatory T cells.
Blood.
ISSN 0006-4971.
118(19),
s. 5141–5151.
doi:
10.1182/blood-2011-02-339242.
Vis sammendrag
Human CD4(+)FoxP3(+)T cells are functionally and phenotypically heterogeneous providing plasticity to immune activation and regulation. To better understand the functional dynamics within this subset, we first used a combined strategy of subcellular fractionation and proteomics to describe differences at the protein level between highly purified human CD4(+)CD25(+) and CD4(+)CD25(+) T-cell populations. This identified a set of membrane proteins highly expressed on the cell surface of human regulatory T cells Tregs), including CD71, CD95, CD147, and CD148. CD147 (Basigin or Emmprin) divided CD4(+)CD25(+) cells into distinct subsets. Furthermore, CD147, CD25, FoxP3, and in particular CTLA-4 expression correlated. Phenotypical and functional analyses suggested that CD147 marks the switch between resting (CD45RA(+)) and activated (CD45RO(+)) subsets within the FoxP3(+) T-cell population. Sorting of regulatory T cells into CD147(-) and CD147(+) populations demonstrated that CD147 identifies an activated and highly suppressive CD45RO(+) Treg subset. When analyzing CD4(+) T cells for their cytokine producing potential, CD147 levels grouped the FoxP3(+) subset into 3 categories with different ability to produce IL-2, TNF-alpha, IFN-gamma, and IL-17. Together, this suggests that CD147 is a direct marker for activated Tregs within the CD4(+)FoxP3(+) subset and may provide means to manipulate cells important for immune homeostasis. (Blood. 2011;118(19):5141-5151)
Se alle arbeider i Cristin
-
Landskron, Johannes
(2022).
Basics of Chemical Biology and High throughput screening.
-
Landskron, Johannes
(2022).
Hit Selection.
-
Bengtsen, Mads; Winje, Ivan; Landskron, Johannes; Meza-Zepeda, Leonardi A & Gundersen, Kristian
(2019).
The Epigenetic landscape of two phenotypic extreme skeletal muscles .
-
Bengtsen, Mads; Winje, Ivan; Landskron, Johannes; Meza-Zepeda, Leonardo A. & Gundersen, Kristian
(2019).
The Epigenetic landscape of two phenotypic extreme skeletal muscles .
-
Bengtsen, Mads; Winje, Ivan; Eftestøl, Einar; Domanska, Diana Ewa; Landskron, Johannes & Gundersen, Kristian
(2019).
The Epigenetic landscape of two phenotypic extreme skeletal muscles - soleus and EDL.
Acta Physiologica.
ISSN 1748-1708.
doi:
10.1111/apha.13414.
-
Meås, Hany Zakaria; Haug, Markus; Beckwith, Marianne Sandvold; Louet, Claire; Ryan, Liv & Hu, Z.
[Vis alle 12 forfattere av denne artikkelen]
(2019).
Sensing of HIV-1 by TLR8 activates human T cells and reverses latency.
-
Haug, Markus; Meås, Hany Zakaria; Beckwith, Marianne Sandvold; Louet, Claire; Ryan, Liv & Hu, Z.
[Vis alle 12 forfattere av denne artikkelen]
(2019).
Sensing of HIV-1 by TLR8 in human CD4+ T cells.
Vis sammendrag
Cell-to-cell transmission of HIV can result in endosomal uptake of HIV by CD4+ T cells with possible exposure of viral ssRNA genome to endolysosomal Toll-like receptors (TLRs). TLRs are central in activating inflammatory responses in innate immune cells, but their function in CD4+ T cells is less well understood.
Here we show that combined TLR8 ligand and T cell receptor (TCR) stimulation boosted CD4+ T cell activation by enhancing NF-B and MAPK signaling. This led to increased production of inflammatory cytokines and surface activation marker expression. Adjuvant TLR8 stimulation, but not TLR7 or TLR9, promoted interleukin (IL)-17 and interferon (IFN)- effector cytokine production, indicating lineage commitment towards the pro-inflammatory T helper (Th)1 and Th17 axis.
Using a cell-to-cell transmission model, we found that endosomal HIV induced cytokine secretion from CD4+ T cells, which could be significantly reduced by a TLR8 specific inhibitor. TLR8 engagement also enhanced HIV replication in CD4+ T cells and potentiated the reversal of latency in patient-derived CD4+ T cells. Taken together, our results reveal a previously unknown function of TLR8 in human primary CD4+ T cells in shaping immunity and modulating HIV-1 infection. This adjuvant TLR8 activity can contribute to low-grade inflammation seen in HIV patients and may also have implications for therapeutic targeting and vaccine development.
-
Haug, Markus; Meås, Hany Zakaria; Beckwith, Marianne Sandvold; Louet, Claire; Ryan, Liv & Hu, Z.
[Vis alle 12 forfattere av denne artikkelen]
(2019).
Sensing of HIV-1 nucleic acids by TLR8 in human CD4+ T cells.
Vis sammendrag
During HIV-infection, cell-to-cell transmission results in endosomal uptake of the virus by target CD4+ T cells and potential exposure of the viral ssRNA genome to endolysosomal Toll-like receptors (TLRs). TLRs are instrumental in activating inflammatory responses in innate immune cells, but their function in adaptive immune cells is less well understood.
Here we show that combined TLR8 ligand and T cell receptor (TCR) stimulation boosted CD4+ T cell activation by enhancing NF-kB and MAPK signaling. This led to increased production of inflammatory cytokines and surface activation marker expression. Adjuvant TLR8 stimulation, but not TLR7 or TLR9, promoted interleukin (IL)-17 and interferon (IFN)-g effector cytokine production, indicating lineage commitment towards the pro-inflammatory T helper (Th)1 and Th17 axis. Using a cell-to-cell transmission model, we found that endosomal HIV-1 induced cytokine secretion from CD4+ T cells, which could be significantly reduced by a TLR8 specific inhibitor. TLR8 engagement also enhanced HIV-1 replication in CD4+ T cells and potentiated the reversal of latency in patient-derived CD4+ T cells.
Taken together, our results reveal a previously unknown function of TLR8 in human primary CD4+ T
cells in shaping immunity and modulating HIV-1 infection. This adjuvant TLR8 activity can contribute to low-grade inflammation seen in HIV patients and may have broad implications for therapeutic targeting and vaccine development.
-
-
Meås, Hany Zakaria; Haug, Markus; Beckwith, Marianne; Louet, Claire; Ryan, Liv & Landskron, Johannes
[Vis alle 10 forfattere av denne artikkelen]
(2019).
Sensing of HIV-1 by TLR8 activates human T cells and reverses Latency.
-
Bengtsen, Mads; Winje, Ivan; Landskron, Johannes; Meza-Zepeda, Leonardo A. & Gundersen, Kristian
(2018).
The Epigenetic landscape of two phenotypic extreme skeletal muscles .
-
Meås, Hany Zakaria; Haug, Markus; Beckwith, Marianne; Louet, Claire; Ryan, Liv & Landskron, Johannes
[Vis alle 10 forfattere av denne artikkelen]
(2018).
Sensing of HIV-1 by TLR8 activates human T cells and reverses Latency.
-
Landskron, Johannes
(2017).
Approaches to Find Novel Modulators of Regulatory T Cell Function.
-
Landskron, Johannes
(2017).
Development of high throughput flow cytometry assays.
-
-
Johnsen, Guro Mørk; Landskron, Johannes; Tasken, Kjetil & Staff, Anne Cathrine
(2011).
CHARACTERIZATION OF PLACENTAL AND CIRCULATING REGULATORY T-CELLS.
Placenta.
ISSN 0143-4004.
32(9),
s. A122–A122.
-
Johnsen, Guro Mørk; Landskron, Johannes; Tasken, Kjetil & Staff, Anne Cathrine
(2011).
Characterization of Subsets of T cells and NK cells in Pregnancy and Preeclampsia.
-
Johnsen, Guro Mørk; Landskron, Johannes; Tasken, Kjetil & Staff, Anne Cathrine
(2011).
Characterization of Placental and Circulating Regulatory T-Cells.
Se alle arbeider i Cristin
Publisert
6. mai 2016 19:02
- Sist endret
16. sep. 2022 12:03