Jessica Lönn-Stensrud

• Wiedmer, David; Petersen, Fernanda Cristina; Lönn-Stensrud, Jessica & Tiainen, Hanna (2017). Antibacterial effect of hydrogen peroxide - titanium dioxide suspensions in the decontamination of rough titanium surfaces. Biofouling (Print). ISSN 0892-7014. 33(6), s. 451–459. doi: 10.1080/08927014.2017.1322585. Fulltekst i vitenarkiv Vis sammendrag

  The chemical decontamination of infected dental implants is essential for the successful treatment of peri-implantitis. The aim of this study was to assess the antibacterial effect of a hydrogen peroxide-titanium dioxide (H2O2–TiO2) suspension against Staphylococcus epidermidis biofilms. Titanium (Ti) coins were inoculated with a bioluminescent S. epidermidis strain for 8 h and subsequently exposed to H2O2 with and without TiO2 nanoparticles or chlorhexidine (CHX). Bacterial regrowth, bacterial load and viability after decontamination were analyzed by continuous luminescence monitoring, live/dead staining and scanning electron microscopy. Bacterial regrowth was delayed on surfaces treated with H2O2–TiO2 compared to H2O2. H2O2-based treatments resulted in a lower bacterial load compared to CHX. Few viable bacteria were found on surfaces treated with H2O2 and H2O2–TiO2, which contrasted with a uniform layer of dead bacteria for surfaces treated with CHX. H2O2–TiO2 suspensions could therefore be considered an alternative approach in the decontamination of dental implants. © 2017 Taylor & Francis

• Dragland, Inger Sofie; Rukke, Håkon Valen; Stenhagen, Ida Sofia Refsholt; Lönn-Stensrud, Jessica & Kopperud, Hilde Beate M. (2016). Antibacterial and Antibiofilm Effect of Low Viscosity Chitosan against Staphylococcus epidermidis. International Journal of Microbiology. ISSN 1687-918X. 2016:9159761. doi: 10.1155/2016/9159761. Fulltekst i vitenarkiv Vis sammendrag

  Aim The aim of this study was to investigate the antibacterial and antibiofilm properties of low viscosity chitosan on S. epidermidis growth and biofilm formation. Methods and Results The antibacterial and antibiofilm properties were investigated, during both planktonic growth and biofilm formation. This was performed using different concentrations in media and by coating on polystyrene surfaces. In addition, the bactericidal effect was investigated using a modified direct contact test. The results showed that low viscosity chitosan in media had both a bacteriostatic and bactericidal effect on planktonic growth and biofilm formation of S. epidermidis in a concentration dependent manner. Polystyrene discs coated with chitosan reduced both early biofilm formation (6 h) and late biofilm formation (18 h), as confirmed by scanning electron microscopy. The modified direct contact test showed a bactericidal effect. Conclusion This study demonstrated that low viscosity chitosan has a bacteriostatic and bactericidal activity against S. epidermidis and that the activity is dependent on the amount of chitosan added. In addition, low viscosity chitosan reduced biofilm formation both when added to media and when coated on polystyrene surfaces. Significance and Impact of Study Low viscosity chitosan could be a contribution to new treatment approaches of biofilm-related infections of S. epidermidis.

• Xing, Rui; Witsø, Ingun Lund; Jugowiec, David; Tiainen, Hanna; Shabestari, Maziar & Lyngstadaas, Ståle Petter [Vis alle 8 forfattere av denne artikkelen] (2015). Antibacterial effect of doxycycline-coated dental abutment surfaces. Biomedical Materials. ISSN 1748-6041. 10(5), s. 55003–55012. doi: 10.1088/1748-6041/10/5/055003. Fulltekst i vitenarkiv Vis sammendrag

  Biofilm formation on dental abutment may lead to peri-implant mucositis and subsequent peri-implantitis. These cases are clinically treated with antibiotics such as doxycycline (Doxy). Here we used an electrochemical method of cathodic polarization to coat Doxy onto the outer surface of a dental abutment material. The Doxy-coated surface showed a burst release in phosphate-buffered saline during the first 24 h. However, a significant amount of Doxy remained on the surface for at least 2 weeks especially on a 5 mA–3 h sample with a higher Doxy amount, suggesting both an initial and a long-term bacteriostatic potential of the coated surface. Surface chemistry was analyzed by x-ray photoelectron spectroscopy and secondary ion mass spectrometry. Surface topography was evaluated by field emission scanning electron microscopy and blue-light profilometry. Longer polarization time from 1 h to 5 h and higher current density from 1 to 15 mA cm−2 resulted in a higher amount of Doxy on the surface. The surface was covered by a layer of Doxy less than 100 nm without significant changes in surface topography. The antibacterial property of the Doxy-coated surface was analyzed by biofilm and planktonic growth assays using Staphylococcus epidermidis. Doxy-coated samples reduced both biofilm accumulation and planktonic growth in broth culture, and also inhibited bacterial growth on agar plates. The antibacterial effect was stronger for samples of 5 mA–3 h coated with a higher amount of Doxy compared to that of 1 mA–1 h. Accordingly, an abutment surface coated with Doxy has potential for preventing bacterial colonization when exposed to the oral cavity. Doxy-coating could be a viable way to control peri-implant mucositis and prevent its progression into peri-implantitis.

• Witsø, Ingun Lund; Benneche, Tore; Vestby, Lene Karine; Nesse, Live Lingaas; Lönn-Stensrud, Jessica & Scheie, Anne Aamdal (2014). Thiophenone and Furanone in Control of Escherichia coli O103:H2 Virulence. Pathogens and Disease. 70(3), s. 297–306. doi: 10.1111/2049-632X.12128. Fulltekst i vitenarkiv
• Gustumhaugen, Eva; Lönn-Stensrud, Jessica; Scheie, Anne Aamdal; Lyngstadaas, Ståle Petter; Ekfeldt, Anders & Taxt-Lamolle, Sebastien Francis Michel (2014). Effect of chemical and mechanical debridement techniques on bacterial re-growth on rough titanium surfaces: an in vitro study. Clinical Oral Implants Research. ISSN 0905-7161. 25(6), s. 707–713. doi: 10.1111/clr.12130. Fulltekst i vitenarkiv Vis sammendrag

  Objective The aim of this in vitro study was to compare the effect of combined chemical and mechanical debridement of titanium (Ti) surfaces inoculated with Staphylococcus epidermidis, compared with the effect of chemical debridement alone. Material and Methods Different Ti surfaces were characterized with respect to roughness and subsequently inoculated with S. epidermidis. NaCl (0.9 vol.%), EDTA (12 vol.%), H2O2 (3 vol.%) or H2O2 + TiO2 nanoparticles served as chemical debridement agents, while TiBrush™ was used as the mechanical debridement tool. Safranin staining assessed biomass still attached to surfaces after debridement. Biofilm viability was assessed after re-incubation of the debrided samples. SEM analysis was performed before and after the cleaning process. Results Surface average roughness (Sa) of the samples was measured at 2.22 ± 0.19 μm for group A, 0.19 ± 0.02 μm for group B, and 1.99 ± 0.10 μm for group C. When chemical debridement agents were used alone, H2O2-containing products were most efficient in reducing the biomass load. The surface roughness did not affect the outcome of chemical debridement. However, when combining chemical and mechanical debridement, a further reduction of biofilm load and viability was observed with best effect on the smoothest surface. Conclusions Combining H2O2-containing chemical agents with mechanical debridement (TiBrush™) provided best reduction in biofilm mass and re-growth, when studied in vitro.

• Westas, Emma Carolina A. A.; Gillstedt, Martin; Lönn-Stensrud, Jessica; Bruzell, Ellen & Andersson, Martin (2014). Biofilm formation on nanostructured hydroxyapatite-coated titanium. Journal of Biomedical Materials Research. Part A. ISSN 1549-3296. 102(4), s. 1063–1070. doi: 10.1002/jbm.a.34757. Fulltekst i vitenarkiv Vis sammendrag

  Biofilm formation on medical devices is a common cause of implant failure, especially regarding implants that breach the epithelial tissue, so-called transcutaneous implants. Nanotechnology and the development of new nanomaterials have given the opportunity to design nanotextured implant surfaces. Such surfaces have been studied using various in vitro methods showing that nanosized features strongly benefit bone cell growth. However, little is known on how nanostructured features affect biofilm formation. The aim of this study was therefore to examine the shape- and chemical-dependent effect of a nanostructured hydroxyapatite (HA) coating on the degree of Staphylococcus epidermidis biofilm formation. Three different types of nanosized HA particles having different shapes and calcium to phosphate ratios were compared to uncoated turned titanium using safranin stain in a biofilm assay and confocal laser scanning microscopy (CLSM) for assessment of biofilm biomass and bacterial volume, respectively. No difference in biofilm biomass was detected for the various surfaces after 6 h incubation with S. epidermidis. Additionally, image analysis of CLSM Z-stacks confirmed the biofilm assay and showed similar results. In conclusion, the difference in nanomorphology and chemical composition of the surface coatings did not influence the adhesion and biofilm formation of S. epidermidis. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.

• Lönn-Stensrud, Jessica; Benneche, Tore & Scheie, Anne Aamdal (2012). Kan bakteriers kommunikation öppna för nya infektionsbehandlingar? Läkartidningen. ISSN 0023-7205. 109(17-18), s. 879–881. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Naemi, Ali-Oddin; Benneche, Tore; Petersen, Fernanda Cristina & Scheie, Anne Aamdal (2012). Thiophenones inhibit Staphylococcus epidermidis biofilm formation at nontoxic concentrations. FEMS Immunology and Medical Microbiology. ISSN 0928-8244. 65(2), s. 326–334. doi: 10.1111/j.1574-695X.2012.00964.x. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Benneche, Tore & Scheie, Anne Aamdal (2011). Furanones and Thiophenones in Control of Staphylococcus epidermidis Biofilm Infections? I Mendez-Vilas, Antonio (Red.), Science and Technology against Microbial Pathogens. Research, Development and Evaluation. World Scientific. ISSN 9789814354851. s. 155–159. Fulltekst i vitenarkiv
• Vestby, Lene Karine; Lönn-Stensrud, Jessica; Møretrø, Trond; Langsrud, Solveig; Scheie, Anne Aamdal & Benneche, Tore [Vis alle 7 forfattere av denne artikkelen] (2010). A synthetic furanone potentiates the effect of disinfectants on Salmonella in biofilm. Journal of Applied Microbiology. ISSN 1364-5072. 108(3), s. 771–778. doi: 10.1111/j.1365-2672.2009.04495.x. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Landin, Maria Augusta Dos S Silva; Benneche, Tore; Petersen, Fernanda Cristina & Scheie, Anne Aamdal (2009). Furanones, potential agents for preventing Staphylococcus epidermidis biofilm infections? Journal of Antimicrobial Chemotherapy. ISSN 0305-7453. 63(2), s. 309–316. doi: 10.1093/jac/dkn501. Fulltekst i vitenarkiv
• Benneche, Tore; Hussain, Zainab; Scheie, Anne Aamdal & Lönn-Stensrud, Jessica (2008). Synthesis of 5-(bromomethylene)furan-2(5H)-ones and 3-(bromomethylene)isobenzofuran-1(3H)-ones as inhibitors of microbial quorum sensing. New Journal of Chemistry. ISSN 1144-0546. 32(9), s. 1567–1572. doi: 10.1039/b803926g. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Petersen, Fernanda Cristina; Benneche, Tore & Scheie, Anne Aamdal (2007). Synthetic bromated furanone inhibits autoinducer-2-mediated communication and biofilm formation in oral streptococci. Oral Microbiology and Immunology. ISSN 0902-0055. 22, s. 340–346. Fulltekst i vitenarkiv
• Benneche, Tore; Lønn-Stensrud, Anna Maria Jessica & Scheie, Anne Aamdal (2006). Synthesis of (E)- and (Z)-5-(bromomethylene)furan-2(5H)-one by bromodecarboxylation of (E)-2-(5-oxofuran-2(5H)-ylidene)acetic acid. Synthetic Communications. ISSN 0039-7911. 36. Fulltekst i vitenarkiv

Se alle arbeider i NVA

• Lönn-Stensrud, Jessica (2019). Vi må snakke om bakterier, dine viktigste venner. Kagge Forlag AS. ISBN 9788248924258. 170 s. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2016). Bakterienes forunderlige verden. Universitetsforlaget. ISBN 9788215024967. 176 s. Fulltekst i vitenarkiv

Se alle arbeider i NVA

• Andreassen, Helene N.; Arnesen, Sondre Strandskog; Bertheussen, Lene Elisabeth; Ebeltoft, Nini; Ekanger, Aysa & Faber, Hege Charlotte [Vis alle 15 forfattere av denne artikkelen] (2022). Forskningsintegritet og åpen vitenskap i ph.d.undervisningen: Praktiske spørsmål og etisk refleksjon. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2022). Bakterienes forunderlige verden. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica & Lien, Marianne Inez (2022). Career support framework for early career researchers. Fulltekst i vitenarkiv
• Samuelsen, Anne Berit C. & Lönn-Stensrud, Jessica (2021). Kildehåndtering og kritisk tenkning i det 5-årige masterprogrammet i farmasi. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Skattebu, Julie; Gadmar, Tone Charlotte & Konestabo, Heidi Sjursen (2021). From black screens to success. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2021). KinShip. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2021). Krigen mot bakteriene. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2021). Bakterienes forunderlige verden. Fulltekst i vitenarkiv
• Andreassen, Helene N. & Lönn-Stensrud, Jessica (2021). Forskningsintegritet og åpen vitenskap: Kunnskaper, normer og holdninger blant ph.dkandidater i Norge (RIOS-PhD): Status våren 2021. Fulltekst i vitenarkiv
• Einevoll, Gaute & Lönn-Stensrud, Jessica (2020). Om bakterier. [Internet]. Podcast: Vett og vitenskap med Gaute Einevoll. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica & Grothe, Michael (2020). PhD on track som undervisningsressurs. Fulltekst i vitenarkiv
• N., Andreassen Helene & Lönn-Stensrud, Jessica (2020). Forskningsintegritet og åpen vitenskap: En undersøkelse om kunnskaper, holdninger og normer blant ph.d.-kandidater i Norge ved Helene N. Andreassen, UiT & Jessica Lönn-Stensrud, UiO. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2020). Vi må snakke om bakterier - dine viktigste venner. Fulltekst i vitenarkiv
• Andreassen, Helene N. & Lönn-Stensrud, Jessica (2020). Forskningsintegritet og åpen vitenskap: en undersøkelse om kunnskaper, holdninger og normer blant ph.d.-kandidater i Norge. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2019). Bakteriedama: Hun er livredd for bakterier. Derfor er hun deres største forkjemper. [Internet]. forskning.no. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2019). Norgesglasset, NRK P1. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2019). Studio 2, NRK P2 "Mine fem favorittbakterier". Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2019). Holm, NRK P1+. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2019). God morgen Norge, TV2 "Vi må snakke om bakterier". Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2019). Nitimen, NRK P1. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2019). Studio 2, NRK P2. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2019). Bokomtale: "Vi må snakke om bakterier". [Journal]. Tara nr 16. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2018). God morgen Norge, TV2 "Bakteriefrykt". Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2017). Bakterier og antibiotikaresistens. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2017). Deltakelse på Ekko P2. [Radio]. NRK, Marienlyst, Oslo. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2017). Sjokolade og bakterier hører sammen. Dagbladet. ISSN 0805-3766. Fulltekst i vitenarkiv
• Jemterud, Torkild; Samset, Bjørn Hallvard; Lönn-Stensrud, Jessica & Røyne, Anja (2017). Abels Tårn, NRK P2: "Kan månen ha noe å si når naturkatastrofer på jorda blir utløst? Har du opplevd at eggerøra i matavfallet har begynt å lyse? Har brus med CO2 noen innvirkning på klimaet?". [Radio]. NRK P2. Fulltekst i vitenarkiv
• Sturød, Kjersti; Scheie, Anne Aamdal; Rukke, Håkon Valen & Lönn-Stensrud, Jessica (2016). Skjebnesvangre reiser - ingen vei tilbake? Fulltekst i vitenarkiv
• Wiedmer, David; Tiainen, Hanna; Lönn-Stensrud, Jessica; Petersen, Fernanda Cristina & Haugen, Håvard Jostein (2016). TiO2-H2O2 as decontamination agent - an in vitro study. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2016). Kampen om overflaten. Naturfag : tidsskrift for Nasjonalt nettverk for naturfag. ISSN 1501-8601. 1, s. 58–60. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2016). Antibiotikaresistens - trussel og håp. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2016). Antibiotikaresistens - Den stille pandemien. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2015). Noen av oss må bruke mindre antibiotika. Aftenposten Viten. ISSN 2464-3033. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2015). Antibiotikaresistens - trussel og håp. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2015). Antibiotikaresistens - den stille pandemien. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2015). Antibiotikaresistens - er mirakelmedisinens ære over? Fulltekst i vitenarkiv
• Austrheim, Gunhild; Gullbekk, Eystein & Lönn-Stensrud, Jessica (2015). PhD on Track: på reise med brukerne. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2015). En lommelykt i havet. Naturfag : tidsskrift for Nasjonalt nettverk for naturfag. ISSN 1501-8601. 2, s. 66–67. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2015). Antibiotikaresistens - trussel og håp. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2014). Norden må lede an i kampen mot antibiotikaresistens. Aftenposten Viten. ISSN 2464-3033. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2014). Veien til ny mirakelmedisin. Aftenposten Viten. ISSN 2464-3033. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2014). Etter mirakelmedisinen. Aftenposten Viten. ISSN 2464-3033. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica & Gullbekk, Eystein (2014). PhD on track – working with generic skills in context. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2014). Small talk. Cell-to-cell communication in bacteria. Fulltekst i vitenarkiv
• Witsø, Ingun Lund; Rukke, Håkon Valen; Assev, Synnøve; Benneche, Tore; Lönn-Stensrud, Jessica & Scheie, Anne Aamdal (2014). Structure-activity relationships in thiophenone. Fulltekst i vitenarkiv
• Lundmark, Anders Mattias & Lönn-Stensrud, Jessica (2014). Launching high school teaching support at the University of Oslo Science Library. Fulltekst i vitenarkiv
• Witsö, I.L.; Benneche, Tore; Nesse, Live L.; Vestby, Lene Karine; Lönn-Stensrud, Jessica & Scheie, Anne Aamdal (2012). Thiophenone TF101 in control of E. coli biofilm? Fulltekst i vitenarkiv
• Gustumhaugen, Eva; Lönn-Stensrud, Jessica; Scheie, Anne Aamdal; Lyngstadaas, Ståle Petter & Taxt-Lamolle, Sebastien Francis Michel (2012). Chemical and Mechanical Debridement of Rough Titanium Surfaces in vitro. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2011). Synthetic furanones and inhibition of biofilm formation. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Naemi, Ali-Oddin; Assev, Synnøve; Benneche, Tore & Scheie, Anne Aamdal (2011). New synthetic thiophenones inhibit biofilm formation by S. epidermidis. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2010). Furanones, potential agents for preventing Staphylococcus epidermidis biofilm infections? Fulltekst i vitenarkiv
• Vestby, Lene Karine; Møretrø, Trond; Ballance, Simon; Benneche, Tore; Scheie, Anne Aamdal & Lönn-Stensrud, Jessica [Vis alle 8 forfattere av denne artikkelen] (2010). Persistence and eradication of Salmonella from the feed environment. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Benneche, Tore & Scheie, Anne Aamdal (2010). Furanones and thiophenones in control of S. epidermidis biofilm infections? Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2010). Furanones and thiophenones in control of S. epidermidis biofilm infections? Fulltekst i vitenarkiv
• Vestby, Lene K.; Lönn-Stensrud, Jessica; Møretrø, Trond; Langsrud, Solveig; Scheie, Anne Aamdal & Benneche, Tore [Vis alle 7 forfattere av denne artikkelen] (2009). The effect of a synthetic furanone and disinfectants on Salmonella in biofilm. Fulltekst i vitenarkiv
• Vestby, Lene K.; Lönn-Stensrud, Jessica; Møretrø, Trond; Langsrud, Solveig; Scheie, Anne Aamdal & Benneche, Tore [Vis alle 7 forfattere av denne artikkelen] (2009). The effect of a synthetic furanone and disinfectants on Salmonella in biofilm. Fulltekst i vitenarkiv
• Vestby, Lene Karine; Lönn-Stensrud, Jessica; Møretrø, Trond; Langsrud, Solveig; Scheie, Anne Aamdal & Benneche, Tore [Vis alle 7 forfattere av denne artikkelen] (2009). The effect of a synthetic furanone and disinfectants on Salmonella in biofilm. Fulltekst i vitenarkiv
• Vestby, Lene Karine; Lönn-Stensrud, Jessica; Møretrø, Trond; Langsrud, Solveig; Scheie, Anne Aamdal & Benneche, Tore [Vis alle 7 forfattere av denne artikkelen] (2009). The effect of a synthetic furanone and disinfectants on Salmonella in biofilm. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2008). Furanones: tools for controlling biofilm infections. Fulltekst i vitenarkiv
• Vestby, Lene Karine; Nesse, Live Lingaas; Lönn-Stensrud, Jessica; Petersen, Fernanda Cristina; Møretrø, Trond & Langsrud, Solveig [Vis alle 8 forfattere av denne artikkelen] (2008). AI-2 production by different wild-type Salmonella strains and the effect of biofilm formation by introducing AHL, GMP and a synthetic bromated furanones. Fulltekst i vitenarkiv
• Nesse, Live; Vestby, Lene K.; Lönn-Stensrud, Jessica; Petersen, Fernanda Cristina; Møretrø, Trond & Langsrud, Solveig [Vis alle 8 forfattere av denne artikkelen] (2008). The effect of a synthetic bromated furanone on the production of biofilm by different Salmonella wild type strains. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Benneche, Tore; Petersen, Fernanda Cristina & Scheie, Anne Aamdal (2007). Biofilm formation in Staphylococcus epidermidis is inhibited by synthetic bromated furanones. Fulltekst i vitenarkiv
• Vestby, Lene K.; Lönn-Stensrud, Jessica; Petersen, Fernanda Cristina; Scheie, Anne Aamdal; Møretrø, Trond & Langsrud, Solveig [Vis alle 7 forfattere av denne artikkelen] (2007). The role of autoinducer-2 in biofilm formaion by salmonella. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Benneche, Tore; Petersen, Fernanda Cristina & Scheie, Anne Aamdal (2007). Furanones Inhibit AI-2 Mediated Communication in Bacteria at Non-Irritant Concentrations. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Benneche, Tore; Petersen, Fernanda Cristina & Scheie, Anne Aamdal (2007). AI-2 Mediated Biofilm Formation in Oral Streptococci is Inhibited by Bromated Furanone. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Benneche, Tore; Petersen, Fernanda Cristina & Scheie, Anne Aamdal (2007). AI-2 Mediated Biofilm Formation in Oral Streptococci is Inhibited by Bromated Furanone. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Benneche, Tore; Petersen, Fernanda Cristina & Scheie, Anne Aamdal (2007). Bakterier snakker, vi lider. Aftenposten (morgenutg. : trykt utg.). ISSN 0804-3116. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Benneche, Tore; Petersen, Fernanda Cristina & Scheie, Anne Aamdal (2007). Bakteriesnakk gir tanntrøbbel. Forskning.no. ISSN 1891-635X. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Benneche, Tore; Petersen, Fernanda Cristina & Scheie, Anne Aamdal (2007). Biofilm formation in Staphylococcus epidermidis is Inhibited by Synthetic Brominated Furanones. Fulltekst i vitenarkiv Vis sammendrag

  The coagulase-negative staphylococci (CNS) are commensals of human skin and mucous membranes. Staphylococcus epidermidis is the most frequently isolated CNS species, and also one of the major nosocomial pathogens associated with infections on medical implants. The cause of infections is primarily related to biofilm formation on the implant. S. epidermidis infections represent a major burden to the public health system, and are often responsible for prolonged hospitalisation and occasional deaths. Microorganisms in nature prefer a biofilm life style. Biofilm infections are often difficult to treat due to increased resistance both to antibiotics and to the immune system. The algae Delisea pulchra produces bromated furanones that prevent bacterial biofilm formation on its surface. Similar furanones may interfere with both autoinducer-2 and autoinducer-1 signalling in Gram-positive and Gram-negative microorganisms without affecting bacterial growth.

• Lönn-Stensrud, Jessica; Benneche, Tore; Petersen, Fernanda Cristina & Scheie, Anne Aamdal (2006). AI-2 Mediated Communication in Streptococcus anginosus, Streptococcus intermedius and Streptococcus mutans is Inhibited by Bromated Furanone. Fulltekst i vitenarkiv
• Nesse, Live; Vestby, Lene K.; Lönn-Stensrud, Jessica; Møretrø, Trond; Langsrud, Solveig & Scheie, Anne Aamdal [Vis alle 8 forfattere av denne artikkelen] (2006). PRODUCTION OF BIOFILM AND AI-2 IN SALMONELLA STRAINS FROM DIFFERENT SOURCES. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Benneche, Tore; Petersen, Fernanda Cristina & Scheie, Anne Aamdal (2006). AI-2 Mediated Biofilm Formation in Oral Streptococci is Inhibited by Bromated Furanone. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica; Benneche, Tore; Petersen, Fernanda Cristina & Scheie, Anne Aamdal (2006). AI-2 Mediated Communication in Oral Streptococci is Inhibited by Bromated Furanone. Fulltekst i vitenarkiv
• Witsø, Ingun Lund; Scheie, Anne Aamdal & Lönn-Stensrud, Jessica (2015). Quorum sensing interferes with bacterial virulence. Universitetet i Oslo. Fulltekst i vitenarkiv
• Lönn-Stensrud, Jessica (2009). Synthetic furanones and inhibition of biofilm formation. Universitetet i Oslo. Fulltekst i vitenarkiv Vis sammendrag

  I denna studien har vi undersökt om furanoner kan förhindra bildandet av biofilmer. Vår första målsättning var att finna en ny syntesväg för (Z)-5-bromomethylene-2(5H)-furanone, en furanon som produceras naturligt av makro-algen Delisa pulchra, samt att undersöka dess effekt på biofilmer av Streptococcus anginosus, Streptococcus intermedius och Streptococcus mutans. Vi syntetiserade även 5-(bromomethylene)furan-2(5H)-ones och 3- (bromomethylene)isobenzofuran-1(3H)-ones för att vidare kunna undersöka deras effekt på biofilm av Staphylococcus epidermidis. Effekten på biofilm blev undersökt genom att tillsätta furanoner till växtmediet eller genom att observera hur biofilm bildas på en ytadsorberad furanon. Furanonerna minskade effektivt bildandet av biofilm i båda applikationsmetoderna, men ytadsorberad furanon var mest effektiv. Koncentrationerna av furanon som användes hade ingen effekt på bakteriell tillväxt och koncentrationerna låg också långt under de fastställda MIC (Minimal Inhibitory Concentration)-värdena, vilket indikerade att effekten inte var ett resultat av en antimikrobiell effekt. För att kunna bestämma om den inhibitoriska effekten var associerad med furanonernas förmåga att påverka mikrobiell AI-2 kommunikation, inkluderades en mutant som saknar förmåga att kommunicera genom AI-2. Till skillnad från motsvarande vildtyp var mängden biofilm bildad av S. anginosus and S. intermedius mutanterna, som saknar förmåga att kommunicera genom AI-2, inte påverkad av furanoner tillsatta till växtmediet. Mängd bildad biofilm av S. anginosus and S. intermedius mutanterna på ytadsorberad furanon var oförändrad eller endast delvis påverkad hos de respektive mutanterna. Genom att tillsätta DPD, den syntetiska AI-2 kommunikationssignalen, till växtmediet kunde den inhibitoriska effekten av furanon upphävas. Vidare fann vi att furanon minskade bioluminescence hos AI-2 reporter-stammen. Tillsammans understöder resultaten antagandet att furanoner påverkar mikrobiell kommunikation utan att påverka mikrobiell tillväxt. Slutligen undersökte vi möjliga skadliga och genotoxiska effekter av furanoner. Först efter att ha använt 1000 gånger högre koncentrationer än de vi använde i biofilm-försöken såg vi en skadlig effekt. Vi såg inga signifikanta förändringar i våra genotoxiska tester hos möss som blivit behandlade med furanon. Möjligheten att kunna påverka bakteriell kommunikation kan därför representera en ny och lovande strategi för att kunna kontrollera bakteriella biofilmrelaterade infektioner.

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