Flow Cytometry Core Facility
The Flow Cytometry Core Facility (FCCF) provides flow cytometry analysis and cell sorting services.
Illustration: Idun Dale Rein
We offer a range of services, including sample analysis and cell sorting, as well as instrumentation, software, and technical expertise/advice for a variety of flow cytometric protocols.
We provide sorting of cells, bacteria, chromosomes, beads and other micro organisms based on light scatter and/or fluorescent markers. Cells can be sorted under sterile conditions and with high purity enabling the recovered cells to be cultured. It is possible to sort cells according to antigen/flourescent protein expression, DNA content or cell function, or a combination thereof.
Cells can also be sorted to retrieve sub-populations for RNA and protein extraction or for morphological analysis. Sorted cells can be collected into tubes (one to four tubes/populations) or deposited as single cells into multiwell plates or onto slides. This can be useful for cloning transfected cells, hybridomas, PCR, FISH, etc. The sorting can be optimized for purity, recovery or count accuracy.
We also provide training and assistance for flow cytometry analysis of samples as well as technical expertise and advice for experimental planning and design, cell preparation, analysis and data handling.
The facility is divided into three locations at the Oslo University Hospital Oslo: Montebello, Gaustad and Ullevål.
Funding and organization
The flow cytometry core facility is a non-profit facility and is supported by grants from Helse Sør-Øst, OUS and UiO, as well as nominal user fees. Services are provided primarily for academic users from the Helse Sør-Øst region. In addition, other interested national and international academic users as well as commercial actors are encouraged to contact FCCF management/staff to contract services.
More information can be found in the Flow Cytometry Core Facility web pages (OUS).