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Electron Microscopy Laboratory

The electron microscopy core facility at IMB provides state of the art transmission electron microscopy (TEM) service. Our expertise dates back to 40 years of experience in implementing the use of electron microscopy techniques in biomedical research.

Left: Post-embedding electron microscopic immunogold cytochemistry showing aquaporin-4 (AQP4) immunogold labeling (black dots) along the glial lamellae in the subfornical organ of rat (Photo: Erlend Nagelhus, J neuroscience 1997) Right: Electron micrograph showing a brain capillary surrounded  by glial and neuronal compartments in a mouse brain section contrasted with uranyl acetate and lead citrate (Photo: Shreyas B. Rao, 2019)

Left: Post-embedding electron microscopic immunogold cytochemistry showing aquaporin-4 (AQP4) immunogold labeling (black dots) along the glial lamellae in the subfornical organ of rat (Photo: Erlend Nagelhus, J neuroscience 1997) Right: Electron micrograph showing a brain capillary surrounded  by glial and neuronal compartments in a mouse brain section contrasted with uranyl acetate and lead citrate (Photo: Shreyas B. Rao, 2019)

TEM enables detailed knowledge about the molecular localization, structure and morphology of biological material at nanometer scale. We have a long tradition of pre-and postembedding techniques, and our main expertise lies in obtaining excellent ultrastructure along with good quality staining and labelling of biological samples.

With advanced techniques such as immunogold cytochemistry, we are able to localize and quantify molecules within biological samples. For quantification of gold particles, we use a tailor-made software developed in house.

Equipment

The facility operates two TEMs from FEI Company

  • Tecnai G2 Spirit BioTwin
  • Tecnai 12 BioTwin (upgraded to G2)

Both electron microscopes are equipped with a fully motorized computer controlled stage and a side mounted Veleta 2kX2k CCD camera. The camera has iTEM software from Olympus Soft Imaging Solutions for acquiring digital images.

Available specimen holders:

  • Standard single tilt holder
  • Double tilt holder
  • Rotation holder
  • Multiple specimen holder
  • Gatan Cryo holder
  • Services and prices

Bashir Hakim, our service engineer, provides user training and assistance as well as full technical maintenance of the microscopes.

Our technical expertise in a vast range of different techniques can be adapted to individual projects. The methods include preparation of biological specimen in different resins such as Lowicryl® HM20, using a new automated freeze substitution system (Leica EM AFS2), and Durcupan. Additionally, we provide ultramicrotome sectioning, immunogold labelling and analysis as well as training in the techniques. For more information about services and prices, see our price list.

Our services are available to researchers at UiO, OUS, and Helse Sør-Øst, as well as external companies and research groups both nationally and internationally.

Location

You can find us at the Division of Anatomy, Domus Medica

Published Feb. 24, 2017 11:08 AM - Last modified Nov. 17, 2020 11:04 AM