Global analyses are well suited for generating hypothesis. For samples to be analyzed by global metabolomics and lipidomics, study design, and the quality of the samples, is of great importance in obtaining reliable data for interpretation.
Contact us to and we will help you evaluate if global metabolomics and/or global lipidomics is a suitable tool for answering your scientific question.
Services
- help in setting up study design
- sample preparation
- global metabolomics and global lipidomics analysis
- data processing
- interpretation of results
We have two liquid chromatography – high resolution mass spectrometry instruments (Dionex Ultimate 3000 HPLC-Q Exactive Orbitrap, and Vanquish Horizon HPLC-Orbitrap Fusion Tribrid). For sample preparation we have all equipment needed including a manual puncher for dried blood spots, centrifuges, tissue homogeniser, thermomixers and vortex.
We have competence in global metabolomics and global lipidomics analysis of dried blood spots, plasma, serum, urine, cerebrospinal fluid, tear fluid, bile.
For other types of sample material please contact us and we will find a way. All sample materials are analyzed using the same analytical method (global metabolomics or global lipidomics). This approach enables us to use our in-house library of about 1000 compounds for identification of compounds detected, regardless of type of material analyzed.
For identification of compounds not included in our steadily growing in-house library we have a number of different tools available.
To initiate a project with us, please send us (to the email below) the filled-out questionnaire, which allows us to understand your needs. We will evaluate your request and reply within a week.
Prices
| Service | Cost (not including VAT) |
| Evaluation of the project questionnaire | Free of charge |
| Pilot experiment | 30 000 NOK* |
| Method and sample preparation optimization (if needed) | To be individually discussed |
| Global metabolomic analysis | 3000 NOK per sample** |
| Global lipidomics analysis | 3000 NOK per sample** |
| Minimal cost of a standard project | 90 000 NOK (equals 30 samples analyzed by metabolomics or lipidomics). If fewer samples are analyzed the cost will not go below this price.** |
| Special requests for additional compounds of interest | The costs of the standards and additional work involving the identification of compounds not already present in our large in-house library will be charged upon agreement*** |
| Extensive involvement in the identification of compounds of interest, in-depth biochemical interpretation or advanced statistical analysis and visualisations. | Individually assessed costs**** |
* Pilot projects are aimed for feasibility testing or experimental conditions optimization. Results from the pilot projects are not intended for publication purposes.
** Services included in the standard project
- Help with the design of the experiment
- Sample preparation
- Analysis in the positive ionization mode
- Analysis in the negative ionization mode
- All consumables
- Data processing and statistical evaluation by the Compound Discoverer (3.5 or newer)
- Annotation of differently abundant compounds assigned with a level of confidence
- Investigation of pre-defined list of compounds (if requested)
- Report of the results
- Data tables and raw data (if requested)
- Deposition of raw data to repositories (if requested)
Services not included in the standard project
- Extensive annotation of compounds (e.g. efforts of annotating majority of signals, manual searching through online libraries, advanced in silico predictions).
- Advanced statistical evaluation and advanced visualisation of the data
- Advanced biochemical interpretation of the results
*** In the case of compounds of interest or differentially abundant compounds not included in our library, the standards of these analytes can be purchased, and these compounds can be included in the analysis or to confirm Level 1 annotation upon agreement.
**** If the customer requests extensive compound identification, biochemical interpretation, advanced statistical evaluation or visualisations of the metabolomic or lipidomic data, additional costs will have to be charged at the request of the customer (hourly rate of NOK 900 or a fixed price agreed). Co-authorship of subsequent scientific publications resulting from the extensive evaluation of the metabolomic or lipidomic analyses can be considered upon approval of the customer and the head of the Core facility for global metabolomics and lipidomics.
Contact
Core facility leader: Katja B P Elgstøen